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Molecular identification of species in Prunus sect. Persica (Rosaceae), with emphasis on evaluation of candidate barcodes for plants

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Journal of Systematics and Evolution 2011年第2期49卷 138-145页

作者： 1,2.Xu QUAN 1 Shi-Liang ZHOU 1 (State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, chinese academy of {2., beijing 100093, china) 2.(graduate School of the chinese academy of {2., beijing 100049, china) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (graduate School of the chinese {2. of sciences Beijing 100049 China)

Species of Prunus L. sect. Persica are not only important fruit trees, but also popular ornamental and medicinal plants. Correct identification of seedlings, barks, or fruit kernels is sometimes required, but no reliable morphological characters are available. Nowadays, the technique of DNA barcoding has the potential to meet such requirements. In this study, we evaluated the suitability of 11 DNA loci (atpB-rbcL, trnH-psbA, trnL-F, trnS-G, atpF-H, rbcL, matK, rpoB, rpoC1, nad1, and internal transcribed spacer [ITS]) as candidate DNA barcodes for peaches, using samples from 38 populations, covering all the species in sect. Persica. On the whole, the primers worked well in this group and sequencing difficulties were met only in the case of ITS locus. Five loci (rbcL, matK, rpoB, rpoC, and nad1) have very low variation rates, whereas atpB-rbcL, atpF-H, trnH-psbA, trnL-F and trnS-G show more variability. The most variable loci, atpB-rbcL and trnH-psbA, can distinguish three of the five species. Two two-locus combinations, atpB-rbcL+trnL-F and atpB-rbcL+atpF-H, can resolve all five species. We also find that identification powers of the loci are method-dependent. The NeighborNet method shows higher species identification power than maximum parsimony, neighbor joining, and unweighted pair group method with arithmetic mean methods.

关键词： atpB-rbcL DNA barcode Prunus sect. Persica trnL-F.

Application of the moist vorticity vector in the analysis of a heavy rainfall event in North china

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Progress in Natural Science:Materials International 2007年第12期17卷 1457-1465页

作者： Zhao Yu1,2.3, Wang Jianguo4 and Xue Deqiang2.1. Laboratory of Cloud-Precipitation Physics and Severe Storms, Institute of Atmospheric Physics, chinese academy of {2., beijing 10002., china 2. Shandong Provincial Meteorological Observatory, Jinan 2.0031, china 3. graduate university of chinese academy of {2., beijing 100039, china 4. Shandong Provincial Meteorological Bureau, Jinan 2.0031, china) Laboratory of Cloud-Precipitation Physics and Severe Storms Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Shandong Provincial Meteorological Observatory Jinan 250031 China graduate university Chinese Academy of Sciences Beijing 100039 China Shandong Provincial Meteorological Bureau Jinan 250031 China

The moist vorticity vector (MVV), defined as (ζa×▽qv)/ρ introduced by Gao et al. is used to study a heavy rainfall event in North china. The MVV has zonal, meridional and vertical components in a three-dimensional framework. Analysis of domain-mean and mass-integrated quantities shows that the variation of the vertical component of the MVV closely follows the variation of the cloud hydrometeors, with a linear correlation coefficient of 0.95, indicating that the vertical component of the MVV is strongly associated with midlatitude convection. Further analysis shows that the vertical component of the MVV reflects the interaction between the horizontal vorticity and the horizontal specific humidity gradient, which can associate dynamic and thermodynamic processes with cloud microphysical processes. Thus, this study can help to understand how the interaction between circulations and clouds aids the development of convection, and the MVV can be used to trace the development and evolution of heavy rainfall.

关键词： moist vorticity vector heavy rainfall modeling study.

Overexpression of a tobacco small G protein gene NtRop1 causes salt sensitivity and hydrogen peroxide production in transgenic plants

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Science china(Life sciences) 2008年第5期51卷 383-390页

作者： CAO YangRong1,2. LI ZhiGang1,2. CHEN Tao1,2. ZHANG ZhiGang1, ZHANG JinSong1 & CHEN ShouYi1 1 National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, chinese academy of {2., beijing 100101, china 2.graduate School of chinese academy of {2., beijing 100039, china National Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China graduate School of chinese {2. of sciences Beijing China

The small GTPases of Rop/Rho family is central regulators of important cellular processes in plants. Tobacco small G protein gene NtRop1 has been isolated; however, its roles in stress responses were unknown. In the present study, the genomic sequence of NtRop1 was cloned, which has seven exons and six introns, similar to the Rop gene structure from Arabidopsis. The NtRop1 gene was constitutively expressed in the different organs whereas the other six Rop genes from tobacco had differential expression patterns. The expression of the NtRop1 gene was moderately induced by methyl viologen, NaCl, and ACC treatments, but slightly inhibited by ABA treatment, with no significant induction by NAA treatment. The transgenic Arabidopsis plants overexpressing the NtRop1 showed increased salt sensitivity as can be seen from the reduced root growth and elevated relative electrolyte leakage. The hydrogen peroxide production was also promoted in the NtRop1-trangenic plants in comparison with wild type plants. These results imply that the NtRop1 may confer salt sensitivity through activation of H2.2.production during plant response to salt stress.

关键词： small G protein NtRop1 salt sensitive hydrogen peroxide

Polynomial regression calculation of the Earth's position based on millisecond pulsar timing

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Research in Astronomy and Astrophysics 2012年第2期12卷 219-234页

作者： Feng Tian 1,2.Zheng-Hong Tang 1,Qing-Zeng Yan 1,2.and Yong Yu 11 Shanghai Astronomical Observatory,chinese academy of {2.,Shanghai 2.0030,china 2.graduate university of chinese academy of {2.,beijing 100049,china Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China graduate university of {2. academy of sciences Beijing 100049 China

Prior to achieving high precision navigation of a spacecraft using X-ray observations, a pulsar rotation model must be built and analysis of the precise posi- tion of the Earth should be performed using ground pulsar timing observations. We can simulate time-of-arrival ground observation data close to actual observed values before using pulsar timing observation data. Considering the correlation between the Earth＇s position and its short arc section of an orbit, we use polynomial regression to build the correlation. Regression coefficients can be calculated using the least square method, and a coordinate component series can also be obtained; that is, we can calcu- late Earth＇s position in the Barycentric Celestial Reference System according to pulse arrival time data and a precise pulsar rotation model. In order to set appropriate param- eters before the actual timing observations for Earth positioning, we can calculate the influence of the spatial distribution of pulsars on errors in the positioning result and the influence of error source variation on positioning by simulation. It is significant that the threshold values of the observation and systematic errors can be established before an actual observation occurs; namely, we can determine the observation mode with small errors and reject the observed data with big errors, thus improving the positioning result.

关键词： autonomous positioning - millisecond pulsar - pulsar timing - re-gression analysis

SAR IMAGING SIMULATION OF HORIZONTAL FULLY TWO-DIMENSIONAL INTERNAL WAVES

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Journal of Hydrodynamics 2006年第3期18卷 294-302页

作者： SHEN Hui HE Yi-Jun Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China graduate School of the chinese {2. of sciences Beijing 100039 China

Based on the research of Lynett and Liu, a new horizontal fully two-dimensional internal wave propagation model with rotation effect was deduced, which can be used to simulate the characteristics of internal waves in a horizontal fully two-dimensional plane. By combining the imaging mechanism of Synthetic Aperture Radar（SAR）, a simulation procedure was fatherly acquired, which can simulate the propagation characteristics of oceanic internal waves into SAR images. In order to evaluate the validity of the proposed simulation procedure, case studies are performed in South china Sea and results from simulation procedure are analyzed in detail. A very good consistency was found between the simulation results and satellite images. The proposed simulation procedure will be a possible foundation for the quantitative interpretation of internal waves from fully two-dimensional satellite images.

关键词： oceanic internal waves imaging simulation,Synthetic Aperture Radar (SAR)

Influence of the earth rotation on the interfacial wave solutions and wave-induced tangential stress in a two-layer fluid system

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Acta Oceanologica Sinica 2009年第2期28卷 115-120页

作者： SONG Jinbao LIU Yongjun Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Key Laboratory of Ocean Circulation and Waves （KLOCAW） Chinese Academy of Sciences Qingdao 266071 China graduate university of {2. academy of sciences Beijing 100039 China

Interfacial waves and wave-induced tangential stress are studied for geostrophic small amplitude waves of two-layer fluid with a top free surface and a fiat bottom. The solutions were deduced from the general form of linear fluid dynamic equations of two-layer fluid under the f-plane approximation, and wave-induced tangential stress were estimated based on the solutions obtained. As expected, the solutions derived from the present work include as special cases those obtained by Sun et al. （2.04. Science in china, Ser. D, 47（12.： 1147-1154） for geostrophic small amplitude surface wave solutions and wave-induced tangential stress if the density of the upper layer is much smaller than that of the lower layer. The results show that the interface and the surface will oscillate synchronously, and the influence of the earth＇s rotation both on the surface wave solutions and the interfacial wave solutions should be considered.

关键词： two-layer fluid interfacial waves wave-induced tangential stress earth rotation

Aridity/humidity status of land surface in china during the last three decades

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Science china Earth sciences 2005年第9期48卷 1510-1518页

作者： WU Shaohong1, YIN Yunhe1,2. ZHENG Du1 & YANG Qinye1 1. Institute of Geographical sciences and Natural Resources Research, chinese {2. of sciences (CAS), beijing 100101, china 2. graduate School of the CAS, beijing 100039, china Institute of Geographical sciences and Natural Resources Research Chinese Academy of Sciences (CAS) Beijing China graduate School of the CAS Beijing China

To clarify aridity/humidity status of land surface is helpful for studying environmental background and regional differences, seeking causes of environmental change, and providing a scientific basis for researches on climate change in the future. In this paper, the authors calcu- lated potential evapotranspiration of china using data from 616 meteorological stations during the period of 1971―2.00 with the Penman-Monteith model recommanded by FAO in 1998. Vy- sothkii’s model was used to calculate aridity/humidity index. Then the calculated results of sta- tions were interpolated to land surface using ArcGIS. Results show that the annual average po- tential evapotranspiration is 400―1500 mm in the whole country, 600―800 mm in most parts of it; and 350―1400 mm in growing season (April―Octobor), which is nearly 2.0 mm less than the annual average. According to the aridity/humidity indexes of 1.0, 1.5 and 4.0, the aridity/humidity status is categorized to four types, namely, humid, subhumid, semiarid and arid. A majority of stations (76%) are more humid in growing season than the annual average. Results of com- prisons between the distribution map of aridity/humidity index with that of precipitation and vegetation indicate a good consistence of aridity/humidity status with natural environment. Therefore potential evapotranspiration calculated with modified FAO’s Penman-Monteith model in combination with aridity/humidity index that considers water balance can more reasonably explain the actual land surface aridity/humidity status of china.

关键词： potential evapotranspiration, aridity/humidity status, land surface, regional differences, Penman-Monteith.

A new approach of cascade utilization of the chemical energy of fuel

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Progress in Natural Science:Materials International 2006年第5期16卷 518-523页

作者： HAN Wei, JIN Hongguang and LIN Rumou ( Institute of Engineering Thermophysics, chinese academy of {2., beijing 100080, china graduate School of the chinese academy of {2., beijing 100080, china) Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing 100080 China graduate School of the chinese {2. of sciences Beijing 100080 China

The indirect release of chemical energy of fuel is investigated, and a new mechanism is proposed to identify the cascade utilization of chemical energy of fuel more clearly. Based on the concept of energy level, the internal phenomenon of the indirect chemical energy release is disclosed, and the equations of energy level describing the utilization of chemical energy and thermal energy during the indirect chemical energy release process are obtained. From theoretical analysis, we find that the superiority of the indirect chemical energy release of fuel comes from the cascade utilization of the fuel's chemical energy. Moreover, the cascade utilization of chemical energy is verified by the investigation of CRGT (chemically recuperated gas turbine). As a result, the thermal exergy obtained from the chemical energy release of fuel increases by 2.% -3 % . The results obtained here may help a deeper understanding of indirect chemical energy release of fuel and provide a theoretical basis for the synthesis of innovative energy systems.

关键词： chemically recuperated gas turbine methane/steam reforming chemical energy level cascade utilization.

Estimation of forest volumes by integrating Landsat TM imagery and forest inventory data

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Science china(Technological sciences) 2006年第Z1期49卷 54-62页

作者： GU Huiyan, DAI Limin, WU Gang, XU Dong, WANG Shunzhong &WANG Hui Institute of Applied Ecology, chinese academy of {2., Shenyang 110016, china School of Forestry, Northeast Forestry university, Harbin 150040, china State Key Lab of Systems Ecology, Research Center for Eco-Environmental {2., chinese academy of {2., beijing 100085, china {12. university of chinese academy of {2., beijing 100039, china Institute of Applied Ecology Chinese Academy of Sciences Shenyang China School of Forestry Northeast Forestry University Harbin China State Key Lab of Systems Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China graduate university of {2. academy of sciences Beijing China

Accurate information about forest volumes is essential for forest management planning. The survey interval of the Forest Resource Inventory of china (FRIC) is too long to meet the demand for timely decision-making required for forest protection, management, and utilization. Analysis of satellite imagery provides good potential for more frequent reporting of forest parameters. In this study, we describe an application of the k-nearest neighbors (kNN) method to Landsat TM imagery for improving estimation of forest volumes. Several spectral features were tested and compared in forest volume estimations, including normalized difference vegetation index, environmental vegetation index, and the combination of the spectral features. The combined index resulted in the most accurate volume estimations. The kNN estimator and the combined index were then used in forest volume estimation. The estimation error (RMSE) of the total volume was44.2., much lower than those for Larix forest (the RMSE was 51.7%) and those for the Korean pine and broadleaved forests (the estimation errors were over 71.7% and 88.19%,respectively). This preliminary study demonstrates the potential of forest volume estimations with remote sensing data to provide useful information for forest management if only limited ground information is available.

关键词： forest inventory, landsat TM, k-nearest neighbors, spectral extraction.