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Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2. Zhou Xiuxia1,2. Huang Rong1,2.and Guo Qionglin1(1. Institute of Hydrobiololgy, Chinese Academy of Sciences, Wuhan 430072. china 2. graduate {2. of Chinese Academy of Sciences, Beijing 100039, china) Institute of Hydrobiololgy Chinese Academy of Sciences Wuhan 430072 China graduate university of Chinese Academy of Sciences Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352.amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2.recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

Relationship between surface subsidence factor and mining depth of strip pillar mining

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中国有色金属学会会刊：英文版 2011年第S3期21卷 594-598页

作者： GUO Wen-bing1, 2. HOU Quan-lin 1, ZOU You-feng2.1. College of Earth Science, graduate university of Chinese Academy of Sciences, Beijing 100049,{2. 2. School of Energy Science and Engineering, Henan Polytechnic university, Jiaozuo 454000,{2. Chinese Acad Sci Coll Earth Sci Grad Univ Beijing 100049 Peoples R China Henan Polytech Univ Sch Energy Sci & Engn Jiaozuo 454000 Peoples R China

Strip pillar mining is one of the most important technical measures to control mining subsidence in china. Mining depth is one of the most important factors that can affect the mining subsidence in strip pillar mining. Six numerical simulation models were set up to simulate the surface subsidence of strip pillar mining in different mining depths. The mining depth in these six models ranges from 300 to 800 m. The simulated results were compared and analyzed. The formula was established based on the simulated results. It demonstrated that the subsidence of strip pillar mining method is related to mining depth. While the other conditions are the same, the subsidence of strip pillar mining increases with the increase of mining depth by logarithmic relationship.

关键词： subsidence factor strip pillar mining mining depth numerical simulation

Microcosmic analysis of ductile shearing zones of coal seams of brittle deformation domain in superficial lithosphere

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Science china Earth Sciences 2004年第5期47卷 393-404页

作者： JU Yiwen1,2. WANG Guiliang2. JIANG Bo2.& HOU Quanlin1 1. graduate School, Chinese Academy of Sciences, Beijing 100039, china 2. College of Mineral Resources and Geosciences, china university of Mining & Technology, Xuzhou 2.1008, china Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China china Univ Min & Technol Coll Mineral Resources & Geosci Xuzhou 221008 Peoples R China

The ductile shearing zones of coal seams in a brittle deformation domain in super-ficial lithosphere are put forward based on the study on bedding shearing and ductile rheology of coal seams. The macrocosmic and microcosmic characteristics include wrinkle fold, mymonitized zones and ductile planar structure of coal seams, etc., while the microcosmic characteristics may also include different optic-axis fabrics and the anisotropy of vitrinite reflectance as well as the change of chemical structure and organic geochemistry components. The forming mechanism is analyzed and the strain environment of ductile shearing zones of coal seams discussed. The result indicates that, in the superficial brittle deformation domain, the coal seams are easy to be deformed, resulting in not only brittle deformation but also ductile shearing deformation under the action of force. Because of simple shearing stress, the interlayer gliding or ductile rheology may take place between coal seams and wall rocks. Therefore, many ductile shearing zones come into being in superficial lithosphere (<5 km). The research on ductile shearing zone of brittle de-formation domain in superficial lithosphere is significant not only theoretically for the study of ductile shearing and ductile rheology of the lithosphere but also practically for the structural movement of coal seams, the formation and accumulation of coal-bed methane, and the preven-tion and harness of gas burst in coal mine.

关键词： brittle deformation domain, interlayer gliding, ductile rheology, ductile shearing zones of coal seams, coal mymonite, vitrinite reflectance anisotropy.

A novel compact low-power direct conversion receiver for mobile UHF RFID reader

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Science china(Information Sciences) 2012年第10期55卷 2226-2233页

作者： LIU Shan 1*,WANG Xin'An 1*,SHEN JinPeng 1,WANG Bo 1,YE Tao 2.& HUANG Ru 3 1 Key Laboratory of Integrated Microsystems,Peking university shenzhen {2. School,shenzhen 518055,china 2.Hong Kong R&D Centre for Logistics and Supply Chain Management Enabling Technologies,HongKong,china 3 Institute of Microelectronics,Peking university,Beijing 100871,china 1. Key Laboratory of Integrated Microsystems Peking University Shenzhen Graduate School Shenzhen 518055 China 2. Hong Kong R&D Centre for Logistics and Supply Chain Management Enabling Technologies HongKong China 3. Institute of Microelectronics Peking University Beijing 100871 China

A novel direct conversion receiver with low cost and low power is implemented in a 0.18 μm 1P6M standard CMOS process for a Mobile UHF RFID reader.A highly linear active mixer with low flicker noise and low noise active load is *** efficient and low cost on-chip DC offset voltage canceling scheme is adopted with a high-input-impedance four-input OPAMP to buffer the output of the DC offset canceller(DCOC) *** receiver has a measured input 1 dB compression point of 2.dBm and a sensitivity of 72.dBm in the presence of the large leakage signal from the *** occupying a silicon area of 2.5 mm 2.and consuming 2. mA from a 1.8 V supply,the receiver makes the mobile UHF RFID reader to communicate with a transponder in a distance of 1 m conveniently.

关键词： direct conversion receiver mobile UHF RFID reader low power DCOC active mixer four-input OPAMP

Enhancing oxidative desulfurization of polyoxometalate by integrating with a self-reductive framework

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Science china Materials 2024年第9期67卷 2925-2933页

作者： Yonghui Lin Xiaojun Zhao shenzhen Chang Yixin Zhang Letian Gan Yuyang Tian Qinhe Pan Guangshan Zhu Key Laboratory of Advanced Materials of Tropical Island Resources Ministry of EducationSchool of Chemistry and Chemical EngineeringHainan UniversityHaikou570228China Faculty of Chemistry Northeast Normal UniversityChangchun130024China

With the strict control of sulfur content in fuels,oxidative desulfurization(ODS),a promising desulphurization technology,needs to be continuously *** this study,we integrated multiple approaches(fabricating a porous structure,increasing phosphomolybdic acid(PMo)loading,improving amphiphilicity,and enhancing the intrinsic activity of PMo using a reductive framework)into PAF-54 carriers to improve ODS catalytic *** catalytic performance suggested that PAF-54 was not simply used as a carrier for PMo by physical *** the binding process,electron transfer between PAF-54 and PMo formed Mo^(5+)with superior catalytic *** to the presence of PAF-54,the catalytic activity of PMo as the active component qualitatively improved to achieve rapid and efficient *** importantly,we found that other nitrogen-rich porous organic polymers can also reduce some of Mo^(6+)in PMo during loading,and its formation mechanism was *** work provides a feasible strategy for designing highly efficient DOS catalysts.

关键词： PAF-54 self-reduction oxidative desulfurization Mo^(5+)

Power minimization with derivative constraints for high dynamic GPS interference suppression

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Science china(Information Sciences) 2012年第4期55卷 857-866页

作者： WU RenBiao 1,2.LI Chan 1 & LU Dan 1 1 Tianjin Key Laboratory for Advanced Signal Processing,Civil Aviation university of china,Tianjin 300300,{2. 2.Intelligent Information Institute of ATR Lab,shenzhen university,shenzhen,518060,{2. 1. Tianjin Key Laboratory for Advanced Signal Processing Civil Aviation University of China Tianjin 300300 China2. Intelligent Information Institute of ATR Lab Shenzhen University Shenzhen 518060 China

In this paper,a novel adaptive interference suppression algorithm is proposed for high dynamic GPS (global positioning system) *** new method incorporates derivative constraints on the inter-ference DOA (direction of arrival) into power minimization approach,which could broaden the width of nulls in the interferences directions and adapt to the rapid change of the interference *** with the traditional wide nulling algorithm based on the derivative constraints,the new method does not have to know or estimate the DOAs of the desired signal and the *** new method is firstly discussed in the context of spatial processing,and then extended to space-time adaptive processing to mitigate wideband diffusive multipath *** of the new method is verified via simulation examples.

关键词： GPS interference cancellation adaptive arrays space-time adaptive processing high dynamic GPS receiver

Effect of Fluid Electrical Property Changes on Surface Transverse Wave Sensors

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稀有金属材料与工程 2006年第S3期 514-518页

作者： Chao Zhang~1 Wenyan Wang~2.Yonggui Dong~2.Yan Liu~1 Guanping Feng~1 (1.Research Institute of tsinghua university in shenzhen,shenzhen 518057,china) (2.Department of Precision Instruments and Mechanology,tsinghua university,Beijing 100084,china)

A fluid sensor based on the surface transverse wave (STW) delay line on ST-cut quartz has been developed and tested in a large number of fluids with different viscosity and permittivity *** of fluid mechanical and electrical properties on the sensor's response has been determined and the sensor's performance has been compared with a bulk acoustic wave (BAW) viscosity *** result shows that the viscosity sensitivity of the developed STW sensor represented by the signal to noise ratio is lower than that of a 5 MHz BAW *** of the sensor in detecting the quality of industrial fluids are discussed.

关键词： surface transverse wave surface acoustic wave fluid sensor electrical property

Properties and applications of cold supersonic gas jet

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 709-715页

作者： ZHANG Shaofeng1,2.MA Xinwen1,2.LIU Huiping2.LI Bin1,2.& ZHU Xiaolong1,2.1. Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,china 2. graduate {2. of Chinese Academy of Sciences,Beijing 100049,china 1. Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China 2. graduate university of Chinese Academy of Sciences Beijing 100049 China

By analyzing the formation mechanism of a supersonic gas jet,a set of equations which describe the atomic beam properties were established. The influence of initial temperature,initial pressure,background gas pressure and pumping speed was discussed in detail. A simulation program was developed based on the equations,and the results under different initial conditions were obtained. The results are in good agreement with the experimental data,and suggest that,in order to get much smaller transverse momentum in collision experiments,it is necessary to lower the initial temperature and the initial pressure of the supersonic gas jet,together with increasing the pumping speed. These results are very instructive for construction of a new generation of cold supersonic gas jets.

关键词： cold supersonic gas jet,zone of silence,nozzle,skimmer.

Exsolution of ilmenite and Cr-Ti magnetite from olivine of garnet-wehrlite

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Science china Earth Sciences 2005年第9期48卷 1368-1376页

作者： LIU Xiangwen1,2. JIN Zhenmin1,3, QU Jing2.& WANG Lu2.1. State Key Laboratory of Geological Processes and Mineral Resources, china university of Geosciences, Wuhan 430074, china 2. graduate School of china university of Geosciences, Wuhan 430074, china 3. Faculty of Earth Sciences, china university of Geosciences, Wuhan 430074, china 1. State Key Laboratory of Geological Processes and Mineral Resources China University of Geosciences 430074 Wuhan China 2. graduate School of china {2. of Geosciences 430074 Wuhan China 3. Faculty of Earth Sciences China University of Geosciences 430074 Wuhan China

Exsolution of rod-like ilmenite (Ilm) and Cr-Ti magnetite (Mt) have been found in olivine of garnet-wehrlite from the core of Chinese Continental Sciences Drilling (CCSD). Their composition, morphology, crystal structure and their topotaxies with host olivine have been studied in detail by the transmission electron microscopy (TEM) and electron probe microanaly- sis (EPMA) technique. It shows that rod-like Ilm exsolution reported in this paper has similar characteristic with that of Alpe Arami olivine, while Cr-Ti magnetite exsolution mentioned in this paper has large discrepancy with chromite exsolution in Alpe Arami olivine. These observations suggest that both of the exsolutions found in this paper should be solid solution phases in β-olivine at their first period, then experiencing decomposition of solid solution and therefore forming Ilm and Cr-Ti magnetite exsolution with the pressure decreasing. So, this garnet-wehrlite perhaps had been ever located in mantle transition zone with a minimum depth of 300 km.

关键词： garnet-wehrlite, ilmenite, magnetite, exsolution, CCSD.