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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2.WANG Xiuliang 2.GUO Baotai 3,QIAO Lixian 3,DUAN Delin 2.WANG Bin 1 1 The State key laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of {2.s,beijing 100101,china 2.The State key laboratory of Marine Biology,Institute of Oceanology,Chinese Academy of {2.s,Qingdao 2.6071,china 3 College of Life {2.s,Qingdao Agricultural university,Qingdao 2.6109,china 4 {12. of biological {2. and Engineering,School of Chemical and biological Engineering,university of {2. and Technology beijing,beijing 100083,china 1. The State key laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. department of biological {2. and Engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The State key laboratory of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. College of Life sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2.10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942.2. to *** cDNA library was screened with a α-32.p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)

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Cell Research 2004年第5期14卷 407-414页

作者： Ai Xiao LIU*, Shao Bin ZHANG*, Xiao Jing XU, Dong Tao REN, Guo Qin LIU** State key laboratory of Plant Physiology and Biochemistry, College of {2. science, china Agricultural university, beijing 100094, china. State key laboratory of Plant Physiology and Biochemistry College of Biological Science China Agricultural University Beijing 100094 China.

A pea actin isoform PEAc1 with green fluorescent protein (GFP) fusion to its C-terminus and His-tag to its N- terminus, was expressed in prokaryotic cells in soluble form, and highly purified with Ni-Chelating SepharoseTM Fast Flow column. The purified fusion protein (PEAc1-GFP) efficiently inhibited DNase I activities before polymerization, and activated the myosin Mg-ATPase activities after polymerization. The PEAc1-GFP also polymerized into green fluorescent filamentous structures with a critical concentration of 0.75 μM. These filamentous structures were labeled by TRITC-phalloidin, a specific agent for staining actin microfilaments, and identified as having 9 nm diameters by negative staining. These results indicated that PEAc1 preserved the essential characteristics of actin even with His-tag and GFP fusion, suggesting a promising potential to use GFP fusion protein in obtainning soluble plant actin isoform to analyze its physical and biochemical properties in vitro. The PEAc1-GFP was also expressed in tobacco BY2.cells, which offers a new pathway for further studying its distribution and function in vivo.

关键词： actin isoform polymerization DNase I inhibition myosin Mg-ATPase activation expression.

Eliminate indeterminacies of independent component analysis for chemometrics

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Progress in Natural science:Materials International 2008年第8期18卷 1009-1014页

作者： Zhixiang Yao a,b, Kai Zhang c,*, Huanbin Liu b, Hui Su a a department of biological and Chemical Engineering, Guangxi {2. of Technology, Liuzhou 545006, china b State key laboratory of Pulp and Paper-making Engineering, South china {2. of Technology, Guangzhou 510640, china c State key laboratory of Heavy Oil Processing, china {2. of Petroleum, beijing 102.49, china department of biological and Chemical Engineering Guangxi University of Technology Liuzhou 545006 China State key laboratory of Pulp and Paper-making Engineering South China University of Technology Guangzhou 510640 China State key laboratory of Heavy Oil Processing China University of Petroleum Beijing 102249 China

An improved method has been proposed to eliminate the indeterminacies of independent component analysis (ICA) for chemometrics. Following the arrangement of principal components analysis (PCA), the ICA mixing matrix is selected as signal content indexes, and ICA output are sorted and directed. After many times reputations, independent components (ICs) are paired according to the maximum correlation coefficient, and then the mean values of each IC substitutes the original ICs. This indicates that the ICA indeterminacies are eliminated. A simulation example is tested to validate this improvement. Finally, a set of experimental LC-MS data is processed without any prior knowledge or specific limitation and the results show that the improved ICA can directly separate the mixed signals in chemometrics, and it is simpler and more reasonable than the simple to use interactive self-modeling mixture analysis (SIMPLISMA).

关键词： Independent component analysis Indeterminacy in ICA Chemometrics

Detecting robust gene signature through integrated analysis of multiple types of high-throughput data in liver cancer

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Acta Pharmacologica Sinica 2007年第12期28卷 2005-2010页

作者： Xin-yu ZHANG~(2.3,4,6),Tian-tian LI~(5,6),Xiang-jun LIU~(2.3,4,7)~2.department of biological {2. and biotechnology,~3 School of Biomedicine and ~4 Ministry of Education key laboratory of Bioinformatics,tsinghua university beijing 100084,china ~5 laboratory of Medical Genetics,department of Biology,Harbin Medical university,Harbin150086.china department of biological {2. and biotechnology School of Biomedicine and Ministry of Education Key Laboratory of BioinformaticsTsinghua UniversityBeijing 100084China laboratory of Medical Genetics Department of BiologyHarbin Medical Universit

Aim:To investigate the robust gene signature in liver cancer,we applied anintegrated approach to perform a joint analysis of a highly diverse collection ofliver cancer genome-wide datasets,including genomic alterations and transcrip-tion ***:1-class Significance Analysis of Microarrays coupled withranking score method were used to identify the robust gene signature in livertumor ***:In total,1 62. 051 gene expression measurements from 16public microarrays,2.pairs of serial analyses of gene expression experiments,and2.2.loss of heterozygosity reports obtained from 568 publications were used inthis integrated *** resulting robust gene signatures included 90 genes,which may be of great importance to liver cancer research.A system assessmentanalysis revealed that our integrative method had an accuracy of 92. and acorrelation coefficient value of ***:The system assessment resultsindicated that our method had the ability of integrating the datasets from varioustypes of sources,and eliciting more accurate results,as can be very useful in thestudy of liver cancer.

关键词： liver cancer integrated analysis robust gene signature gene expression map microarray SAGE

smad2.and smad3 are required for mesendoderm induction and neural patterning in zebrafish

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smad2 and smad3 are required for mesendoderm induction and n...

Effect of daidzin,genistin,and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts

2.10·第一届斑马鱼学术研讨会

作者： Shunji Jia~1,Di Wu~1,Cencan Xing~1,Anming Meng~(1,2.*) 1.department of biological {2.s and biotechnology,tsinghua university,beijing 100084, china 2.State key laboratory of biomembrane and {12. Engineering,Institute of Zoology, Chinese Academy of {2.s,beijing 100101,china

The *** Nodal,Activin,and Vg1 play important roles in mesendoderm induction and patterning during vertebrate *** ligands are believed to transduce the signal through the receptor-activated transcription factors Smad2....

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Acta Pharmacologica Sinica 2005年第9期26卷 1081-1086页

作者： Xiang-hui LI~1 Jin-chao ZHANG~(2.3) Sen-fang SUI~(1,4) Meng-su YANG~(3,4)1 State key laboratory of {2. department of biological sciences and biotechnology,tsinghua university,beijing 100084,china 2.department of Chemistry College of Chemistry and Environmental science,Hebei university,Baoding 071002.china 3 department of Biology and Chemistry,City university of Hong Kong,Hong Kong,china Statekeylaboratoryof{2. DepartmentofBiologicalSciencesandBiotechnologyTsinghuaUniversityBeijing10008

Aim:To examine the effect of daidzin,genistin,and glycitin on the osteogenic andadipogenic differentiation of bone marrow stromal cells（MSC）and the adipogenictransdifferentiation of ***:MTT test,alkaline phosphatase（ALP）activity measurement,Oil Red O stain and measurement were ***:Daidzin,genistin,and glycitin 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L all promoted the proliferation of primary mouse bone MSC and *** 5×10-7mol/L and genistin 1×10-6mol/L promoted the osteogenesis *** 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L and glycitin 1×10-8,1×10-6,and 1×10-5mol/L inhibited the adipogenesis of ***,genistin,and glycitin 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L all inhibited the adipocytictransdifferentiation of ***:Daidzin,genistin,and glycitinmay modulate differentiation of MSC to cause a lineage shift toward the osteo-blast and away from the adipocytes,and could inhibit adipocytic transdifferen-tiation of *** could also be helpful in preventing the developmentof osteonecrosis.

关键词： daidzin genistin glycitin bone marrow stro-real cells osteogenic differentiation adipogenic differentiation osteoblasts adipocytic transdifferentiation

Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons

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Acta Pharmacologica Sinica 2003年第12期24卷 74-78页

作者： ZHANG Guang-Qin, HAO Xue-Mei, CHEN Shi-Zhong~1, ZHOU Pei-Ai, CHENG He-Ping, WU Cai-Hong~2.State key laboratory of {2. and membrane biotechnology, College of Life sciences, Peking university, beijing 100871 ~1department of Traditional Chinese Medicine, School of Pharmaceutical science, Peking university, beijing 100083,{12. State key laboratory of {2. and membrane biotechnology College of Life Sciences Peking University Beijing 100871

AIM: To study the blockade of paeoniflorin (Pae) on INain the acutely isolated hippocampus neurons of mice. METHODS: The whole-cell patch clamp technique was used. RESULTS: Pae inhibited INain frequency-dependent and concentration-dependent manners, with an IC50of 2.1μmol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal INafrom -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2.mV, respectively, and postponed the recovery of INainactivation state from (4.2.0.7) ms to (9.8±1.2. ms. CONCLUSION: Pae inhibited INain mouse hippocampus neurons.

关键词： hippocampus patch-clamp techniques paeoniflorin sodium channels

Effects of chitosan/collagen substrates on the behavior of rat neural stem cells

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science china(Life sciences) 2010年第2期53卷 215-222页

作者： YANG ZhaoYang1,MO LinHong2.DUAN HongMei2.& Li XiaoGuang1,2.1 School of biological science and Medical Engineering,Beihang {2.,beijing 100191,china 2.beijing Institute for Neuroscience,Capital Medical {2.,beijing 100069,china 1. School of biological science and Medical Engineering Beihang University Beijing 100191 China2. Beijing Institute for Neuroscience Capital Medical University Beijing 100069 China

Spinal cord and brain injuries usually lead to cavity *** transplantation by combining stem cells and tissue engineering scaffolds has the potential to fill the cavities and replace the lost neural *** chitosan and collagen have their unique *** this study,the effects of chitosan and collagen on the behavior of rat neural stem cells (at the neurosphere level) were tested in vitro in terms of cytotoxicity and supporting ability for stem cell survival,proliferation and *** the serum-free condition,both chitosan membranes and collagen gels had low cytotoxicity to *** is,cells migrated from neurospheres,and processes extended out from these neurospheres and the differentiated *** with the above two materials,chitosan-collagen membranes were more suitable for the co-culture with rat neural stem cells,because,except for low cytotoxicity and supporting ability for the cell survival,in this group,a large number of cells were observed to migrate out from neurospheres,and the differentiating percentage from neurospheres into neurons was significantly *** modification of chitosan-collagen membranes may shed light on in vivo nerve regeneration by transplanting neural stem cells.

关键词： chitosan collagen neural stem cells biocompatibility differentiation

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界面对 HeLa 细胞生长与分裂的影响

清华大学学报（自然科学版） 1998年第6期38卷 68-70页

作者：汤青董岩冯辉邢荣志张志诚公衍道张秀芳赵南明清华大学生物科学与技术系

植入体内的生物材料与人体细胞发生作用，这种作用对细胞的生长、分裂呈明显的周期性，其周期又受到胞内胞外诸多因素的影响。一般对细胞生长、分裂的影响作为一个整体看待。ＨｅＬａ细胞接种在有机玻璃（ＰＭＭＡ）、成都科大Ⅱ型聚氨... 详细信息

植入体内的生物材料与人体细胞发生作用，这种作用对细胞的生长、分裂呈明显的周期性，其周期又受到胞内胞外诸多因素的影响。一般对细胞生长、分裂的影响作为一个整体看待。ＨｅＬａ细胞接种在有机玻璃（ＰＭＭＡ）、成都科大Ⅱ型聚氨酯、Ｐｅｌｅｔｈａｎｅ２３６３－５５Ｄ型聚氨酯等材料表面上生长的形态和数量等呈显著差异，而在培养基中加入阿糖胞苷抑制ＤＮＡ合成后，细胞在形态上虽有差异，但数量上趋于相同。结果提示，三种材料对细胞的着床、贴壁和存活生长作用相似，而分裂周期则不同，从而导致细胞生长、分裂整体情况的不同。

关键词：生物材料 HeLa细胞界面细胞生长细胞分裂

维普期刊数据库