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Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2.cells

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Progress in Natural Science:Materials International 2007年第2期17卷 156-162页

作者： CHEN Zhiling, YU Yi, LIU Lina and XIA Guixian ( College of Life Science, Capital normal university, Beijing 100037,{2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080,{2.) [a]College of Life Science Capital Normal University Beijing China [b]Institute of Microbiology Chinese Academy of Sciences Beijing China

The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2.cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48 . The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 2.5-374 and aa 518-646) . Overexpression of NtCDC48 in tobacco BY-2.cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through Gl to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2.cells.

关键词： tobacco BY-2.suspension cells NtCDC48 spindle cell cycle.

A LINEAR THEORY FOR DISTURBANCE OF COHERENT STRUCTURE AND MECHANISM OF SAND WAVES IN OPEN-CHANNEL FLOW

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International Journal of Sediment Research 2001年第2期16卷 234-243页

作者： Yuchuan BAI1, Andreas MALCHEREK2.and Changbo JIANG 1 Institute for Sedimentation on River and Coast Engineering, tianjin university, tianjin 300072. china. E-Mail: Ychbai@*** 2.Federal Waterways Engineering and Research Institute Institute for Sedimentation on River and Coast Engineering Tianjin University Tianjin 300072 China.E-Mail: Ychbai@*** Federal Waterways Engineering and Research Institute Coastal department D-22559 Hamburg Germany.

The formation of sand wave is such a process in which the roughness and discontinuity of the original bed surface cause the disturbance of the bottom laminar flow in an open channel, and the development of the disturbance in turn leads to instability of the flow and the appearance of the coherent structure. The evolution and development of the coherent structure further promote the undulations of bed surface and sand waves rise finally. The sand wave is explained as a result of action that the bed sediment particles are disturbed by the coherent structure. This study shows that the sand wave formation is the result of disturbance action of neutral coherent structure, and the sand wave shape obtained in computations is identical to the practical one.

关键词： Coherent structure Sand wave Open channel flows

SPATIO-TEMPORAL CHANGE OF DROUGHT DISASTER IN china IN RECENT FIFTY YEARS

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Natural Disaster Reduction in china 2003年第1期 11-17页

作者： Wang Jingai 1,2., Sun Heng 1, Xu Wei 1 and Zhou Jinju 1 (1. department of Resource & Environment Science, Beijing normal {2., Beijing 100875, china 2. Key Laboratory of Environmental Change and Natural Disaster, Ministry of Education of china, Beijing normal {2., Beijing 100875, china) SunHui

In this paper, a drought disaster database, based on information from Chinese provincial newspaper, is established. And the spatio temporal pattern of drought disaster from 1949 to 2.00 is reconstructed by using technology of digital mapping. The results show that the spatial pattern of drought disaster takes on discrepancy between the east and west of china, the serious disaster area lies in the north, especially in the west of Heilongjiang, the center of Inner Mongolia, the north of Hebei and Shaanxi, Ningxia; in the south of the country, drought disaster area lies in Anhui, Hubei, Hunan, Jiangxi, Henan, and Guizhou, the east of Sichuan, the center～east of Yunnan. The distribution of drought disaster in china has the tendency of extension to the west. Dynamic change of drought disaster takes on discrepancy between the south and north of the country. The amount of the drought disaster in the north of the Yangtze River is increasing, while the south is decreasing generally or changing invisibly.

关键词： drought disaster amount of the disaster spatio temporal change

Aligned Solidification Structure of the MnBi Phase in Semisolidified Bi-Mn Alloy with a Static Magnetic Field

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Journal of Materials Science & Technology 2004年第3期20卷 311-314页

作者： Zhongming REN Hui WANG Kang DENG Kuangdi XU department of Materials Science and Engineerng,Shanghai university,Shanghai 2.0072.{2. Dept. of Mat. Sci. and Eng. Shanghai Univ. Shanghai 200072 China

The solidification structure of Bi-3 wt pct Mn alloy grown up in the semisolid zone under the influence of a staticmagnetic field (up to 1.0 T) and the relation of the magnetic property with the solidification structure have beeninvestigated experimentally. It was shown that the primary phase MnBi crystals in the alloy aligned and oriented alongthe direction of the applied magnetic field. The orientating tendency and the average length of the elongated MnBicrystals increased with the increase of the applied field and the solidification time. Moreover, the remanence of thealloy along the aligned direction of the MnBi phase in the case of solidification with a magnetic field was apparentlyanisotropic and nearly double of that without the magnetic field. This indicated that the MnBi crystals orientedand aligned along their easy magnetization axis. A model was proposed to explain the alignment and orientationgrowth of the MnBi crystals in a magnetic field in terms of the magnetic anisotropy of the crystals and the magneticinteraction between them.

关键词： Magnetic field Bi-Mn alloy MnBi Solidification

Vibration Analysis of Beams by Spline Finite Element

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沈阳建筑大学学报（自然科学版） 2011年第6期27卷 1005-1012页

作者：杨浩 department of Civil Engineering The University of Hong Kong

In this paper,the spline finite element method is developed to investigate free vibration problems of *** cubic B-spline functions are used to construct the displacement *** assembly of elements and the introduction of boundary conditions follow the standard finite element *** results under various boundary conditions are compared with those obtained by the exact method and the finite difference *** shows that the results are in excellent agreement with the analytical results and much more accurate than the results obtained by the finite difference method,especially for higher order modes.

关键词： vibration analysis beam spline finite element method

DMM:A dynamic memory mapping model for virtual machines

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Science china(Information Sciences) 2010年第6期53卷 1097-1108页

作者： CHEN HaoGang1,WANG XiaoLin1,WANG ZhenLin2.ZHANG BinBin1,LUO YingWei1* & LI XiaoMing11department of Computer Science,Peking university,Beijing 100871,{2. 2.0} of Computer Science,Michigan Technological university,Houghton,MI 49931,USA 1. department of Computer Science Peking University Beijing 100871 China2. Department of Computer Science Michigan Technological University Houghton MI 49931 USA

Memory virtualization is an important part in the design of virtual machine monitors(VMM).In this paper,we proposed dynamic memory mapping(DMM) model,a mechanism that allows the VMM to change the mapping between a virtual machine's physical memory and the underlying hardware resource while the virtual machine is *** utilizing DMM,the VMM can implement many novel memory management policies,such as Demand Paging,Swapping,Ballooning,Memory Sharing and Copy-On-Write,while preserving compatibility with various *** modular and hierarchical property of the DMM model efficiently incorporates the high-level policies and the low-level implementations,leading to a feature-adjustable VMM *** presented the principle of the DMM model,and explained the procedures of various memory management policies under this ***,we implement the DMM model in KVM,an open source *** evaluation shows that the DMM model is efficient enough to provide the benefits of dynamic memory resource management with little performance impact.

关键词： virtual machine monitor Xen virtual machine memory virtualization dynamic memory mapping

Recent developments of nanoparticle-based enrichment methods for mass spectrometric analysis in proteomics

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Science china Chemistry 2010年第4期53卷 695-703页

作者： ZHANG LiJuan, LU HaoJie & YANG PengYuan department of Chemistry and Institutes of Biomedical Sciences, Fudan university, Shanghai 2.0433,{2. 1. department of Chemistry and Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.

关键词： enrichment nanoparticles mass spectrometry proteomics phosphorylation glycosylation

Role of Apolipoprotein A-I in Protecting against Endotoxin Toxicity

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 419-424页

作者： Juan MA, Xue-Ling LIAO, Bin LOU, and Man-Ping WU* department of Biochemistry, School of Pharmacy, Fudan university, Shanghai 2.0032.{2. department of Biochemistry School of Pharmacy Fudan UniversityShanghai 200032 China

High density lipoprotein (HDL) binds lipopolysaccharide (LPS or endotoxin) and neutralizes its toxicity. We investigated the function of Apolipoprotein A-I (ApoA-I), a major apolipoprotein in HDL, in this process. Mouse macrophages were incubated with LPS, LPS+ApoA-I, LPS+ApoA-I+LFF (lipoprotein-free plasma fraction d>1.2.0 g/ml), LPS+HDL, LPS+HDL+LFF, respectively. MTT method was used to detect the mortality of L-92. cells which were attacked by the release-out cytokines in LPS-activated macrophages. It was found that ApoA-I significantly decreased L-92. cells mortality caused by LPS treatment (LPS vs. LPS+ApoA-I, P2.%) and in LPS+ApoA-I+LFF group (10%) was signifi- cantly lower than that in LPS group (80%) (P2. ± 10.13) h in LPS+ApoA-I group and (46.80 ± 3.79) h in LPS+ApoA-I+LFF group, which were significantly longer than that in LPS group (16.2. ± 17.2.) h (P2..35 ± 3.70) (P2. LFF enhances the effect of ApoA-I; (3) ApoA-I is the major contributor for HDL anti-endotoxin function.

关键词： Apolipoprotein-I lipopolysaccharide (LPS) macrophages FITC-lipopolysaccharide (FITC-LPS) L-92. cells

THE CHARACTERISTIC OF MARINE ENVIRONMENT IN LINGDINGYANG ESTUARY

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Chinese Geographical Science 2001年第2期11卷 155-162页

作者： CHEN Bing-lu1, ZHANG Yun-ni2. CHEN Xin-geng1, WANG Zhi-gang1, YANG Guang-xing1 (1.Institute of Environmental Science, Zhongshan university, Guangzhou 5102.5, P. R. china 2. {2. of Environmental Science, Zhongshan university, Guangzhou 5102.5 Institute of Environmental Science Zhongshan University Guangzhou P. R. China department of Environmental Science Zhongshan University Guangzhou P. R. China

Hydrologic features, load of main pollutants and current condition of water quality in Lingdingyang Estuary are discussed by references and monitoring data. Affected by topography, runoff and tide, its dynamic condition is very complicated. Different water areas have different hydrologic features. The topography under the water of Lingdingyang Estuary is higher in the northwest than that in the southeast. The shoal alternates with the deep trough within the bay .The distribution of the salinity and the contents of the mud and the main pollutants tally with the topography tendency. Change in water quality goes through four stages. Current condition of water quality is fairly good. Inorganic nitrogen is the primary pollutant, and then phosphorus. Rich in nutrition of nitrogen and phosphorus become a more and more prominent problem.

关键词： Lingdingyang Estuary hydrologic features load of pollutant water quantity