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Potential control of horseradish peroxidase immobilization on gold electrode

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中国科学：化学英文版 2004年第2期47卷 134-141页

作者： GE Cunwang1,2. YU Wei1, LI Changan1, WANG Nanping2.& GU Ning1 1. Key Laboratory of Molecular and Biomolecular Electronics (Southeast University), Ministry of Education, Research Center for Nano-scale Science and technology, Southeast University, Nanjing 2.0096, China 2. department of Chemistry, Nantong {2. college, Nantong 2.6007, China 1. Key Laboratory of Molecular and Biomolecular Electronics (Southeast University) Ministry of Education Research Center for Nano-scale Science and Technology Southeast University 210096 Nanjing China 2. department of Chemistry Nantong Teachers College 226007 Nantong China

A new approach based on potential control was firstly used for the immobilization of horseradish peroxidase (HRP) as the model protein. The self-assembly monolayer (SAM) was prepared with 2.aminoethanethiol (AET) on the gold electrode. The charge on HRP was adjusted by means of the acidity of the phosphate buffer solution (PBS) for dissolving the HRP. The in-fluence of electric potential on HRP immobilization was investigated by means of colorimetric immunoassay of enzyme-substrate interaction (CIESI) using an automatic plate reader. The HRP modified electrodes were characterized with X-ray photoelectron spectroscopy (XPS) as well as atomic force microscope (AFM) on template-stripped gold surface. The potential for maximum immobilization of HRP was near the zero charge potential. The result indicates that controlled potential can affect the course of HRP immobilization without the loss of enzymic activity. It is of great significance for the control of biomolecular self-assembly and the intrinsic electric device.

关键词： electric potential control, horseradish peroxidase, immobilization, gold electrode, self-assembly.

Tribological Properties of Dimpled Surface Alloying Layer on Carbon Steel

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Journal of Wuhan University of technology(Materials Science) 2009年第2期24卷 218-222页

作者：万轶 department of Materials Science & Engineering Nanjing University of Science and Technology department of Mechanical and Electrical Engineering Yingtian College

The effect of surface structure and coating on tribological properties of 45^# carbon steel disc was analyzed. A Nd：YAG laser was used to generate rnicrodirnples on steel surfaces. Dimples with diameter of 150 rn and depth of 50 rn were distributed in an orbicular array on disc surface. Then the alloying element Mo was sputtered to 45# carbon steel disc surface by means of double glow plasma technology. Diffusion Mo alloying layer with 30min thickness and high hardness up to 0.02. was formed on the disc surface. Tribological experiments of three types samples （smooth, texturing and texturing＋alloying） were conducted with a pin-on-disc tribometer. It is found that the dimpled-samples are most effective for reducing friction in comparison with smooth steel surthces, improving the lubricating state from boundary to hydrodynamic region.

关键词： laser surface texturing double glow plasma surface alloying technology carbon steel tribological performance lubricating state

BGP reflection functors in root categories

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Science China Mathematics 2005年第8期48卷 1033-1045页

作者： XIAO Jie, ZHANG Guanglian1’2.& ZHU Bin department of Mathematical Sciences, Tsinghua University, Beijing 100084, China department of Mathematics, Yancheng {2. college, Yancheng 2.4002. China 1. department of Mathematical Sciences Tsinghua University 100084 Beijing China 2. department of Mathematics Yancheng Teachers College 224002 Yancheng China

We define the BGP-reflection functors in the derived categories and the root categories. By Ringel's Hall algebra approach, the BGP-reflection functor is applicable to obtain the classical Weyl group action on the Lie... 详细信息

关键词： m-cycle complexes, derived categories, BGP-reflections, Kac-Moody algebras.

The construction of infinite families of any k-tight optimal and singular k-tight optimal directed double loop networks

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Science China Mathematics 2007年第7期50卷 1055-1064页

作者： Bao-xing CHEN~(1+) Xie-bin CHEN~2.Ji-xiang MENG~3 Wen-jun XIAO~4 1 department of Computer Science,Zhangzhou teachers {2.,Zhangzhou 363000,China 2.department of Mathematics and Information Science,Zhangzhou teachers {2.,Zhangzhou 363000,China 3 {2. of Mathematics & System Science,Xinjiang University,Urumqi 830046,China 4 department of Computer Science,South China University of technology,Guangzhou 510641,China 1. department of Computer Science Zhangzhou Teachers College Zhangzhou 363000 China 2. department of Mathematics and Information Science Zhangzhou Teachers College Zhangzhou 363000 China 3. college of Mathematics & System Science Xinjiang University Urumqi 830046 China 4. department of Computer Science South China University of Technology Guangzhou 510641 China

The double loop network(DLN)is a circulant digraph with n nodes and outdegree *** is an important topological structure of computer interconnection networks and has been widely used in the designing of local area networks and distributed *** the number n of nodes,how to construct a DLN which has minimum diameter?This problem has attracted great attention.A related and longtime unsolved problem is:for any given non-negative integer k,is there an infinite family of k-tight optimal DLN?In this paper,two main results are obtained:(1)for any k≥0,the infinite families of k-tight optimal DLN can be constructed,where the number n(k,e,c)of their nodes is a polynomial of degree 2.in e with integral coefficients containing a parameter c.(2.for any k≥0, an infinite family of singular k-tight optimal DLN can be constructed.

关键词： double loop network diameter k-tight optimal singular k-tight optimal 05C12 05C2. 05C85

Research of Sports Major Students Management Model in colleges Based on Humanism

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Research of Sports Major Students Management Model in Colleg...

Investigation of nano-talc as a filling material and a reinforcing agent in high density polyethylene (HDPE)

2.11 4th IEEE International Conference on Computer Science and Information technology(ICCSIT 2.11)

作者： Yao Bin-bin Physical Education department of anqing teachers college {2. China

With the social development and higher education reform, students of Anhui PE University have the complex ideas. They brought new management challenges, the traditional management concepts, methods and systems that were not suited to the current student management. To explore new method of management, this paper does a research through literature, expert interviews, questionnaires, statistics, comparative analysis and other methods for some students in physical education of 5 Anhui universities. This paper highlights "people-oriented" advancing with the times. In this paper, people-oriented management principles and details of the management combination with management are analyzed and summarized.

关键词： Sports major open management detailed management

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Rare Metals 2006年第z1期25卷 422-425页

作者： CHEN Nanchun MA Lei ZHANG Tao Center for Material Engineering Guilin Institute of Technology Guilin 541004 China Key Laboratory of Nonferrous Materials and Processing technology Ministry of Education Guilin Institute of Technology Guilin 541004 China Department of Physics and Information Technology Yulin Teachers'' College Yulin 537000 China Center for Material Engineering Guilin Institute of Technology Guilin 541004 China Key Laboratory of Nonferrous Materials and Processing technology Ministry of Education Guilin Institute of Technology Guilin 541004 China

An experiment of producing high density polyethylene (HDPE) nano-composite filled with 4wt.% talc was presented. Acting as filler and a reinforcing agent in the HDPE, talc powder, sized at around 5 μm, was surface-treated with aluminum diethylene glycol dinitrate coupling agent before adding to the HDPE. Analyses of the reinforced HDPE nano-composite show significant improvement in its mechanical properties including, tensile strength (>2. MPa), break elongation (2. MPa), and friction coefficients<0.11. The results demonstrate that, after surface-treated, talc can be used as a promising filling material and a reinforcing agent in making HDPE nano-composite.

关键词： HDPE talc filling material reinforcing agent nano-composite mechanical properties

Research on the coordinated utilization of land resource in the intergrated economic region of industry, farming and grazing-a case study of Baotou city in Inner Mongolia

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Journal of Geographical Sciences 2000年第4期10卷 135-139页

作者： Wu Xianhui1, Guan Zhuojin2. YANG Yu-mei3 (1. department of Resource and Environmental Science, Baotou teachers {2., 014030 2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110015 3.Baotou Medical {2., Baotou 014010, Chin

Baotou City is an industrial city developed in the farming-grazing transitional zone in northern China. The development of industry, farming and grazing is very fast. It has become a typical intergrated economic region of industry, farming and grazing. Based on the analysis of utilization of land resources, this paper points out the main conflicts and problems on the utilization of land resources in the city, and puts forward a corresponding proposal.

关键词： land resource coordinate utilization intergrated economic region Baotou City

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英国职业教育与学习型社会

外国教育研究 2005年第4期32卷 77-80页

作者：黄日强张霞江西科技师范学院职业教育研究所江西南昌330013 东华理工学院教育心理学部江西抚州344000

学习型社会是终身学习、全民学习、开放学习、多样化学习的社会。在这种社会中,职业知识和技能是学习的主要内容,职业教育较之传统社会在教育对象、教育时间和场所、教育形式方面都有了巨大的变化。因应这种时代潮流,英国职业教育作为... 详细信息

学习型社会是终身学习、全民学习、开放学习、多样化学习的社会。在这种社会中,职业知识和技能是学习的主要内容,职业教育较之传统社会在教育对象、教育时间和场所、教育形式方面都有了巨大的变化。因应这种时代潮流,英国职业教育作为学习型社会终身教育的大平台,在推广终身学习理念和实践终身学习理想方面做了一系列有益的尝试。

关键词：英国学习型社会职业教育终身教育

Adsorptive desulfurization on a heteroatoms Y zeolite prepared by secondary synthesis

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Science China Chemistry 2010年第1期53卷 281-286页

作者： TANG Ke1,HONG Xin1,ZHAO YongHua1,SONG LiJuan2.& SUN ZhaoLin2.1School of Material and Chemical Engineering,Liaoning Institute of technology,Jinzhou 12.001,China 2.1} of Petrochemical Engineering,Liaoning University of Petroleum & Chemical technology,Fushun 113001,China 1. School of Material and Chemical Engineering Liaoning Institute of Technology Jinzhou 121001 China2. College of Petrochemical Engineering Liaoning University of Petroleum & Chemical Technology Fushun 113001 China

Gallium atoms have been introduced into the framework of Y zeolite by treating the zeolite with an aqueous solution of ammonium hexafluoro *** of various model fuels containing about 500 μg/g sulfur has been studied over the synthesized Y zeolite(GaAlY).The sulfur adsorption capacity is 7.0,17.4,14.5,16.9,6.9 and 5.8 mg(S)/g adsorbent for thiophene,tetrahydrothiophene(THT),4,6-dimethyldibenzothiophene(4,6-DMDBT),dibenzophiophene(DBT),benzothiophene(BT) and 4-methyldibenzothiophene(4-MDBT),*** charges on the S atom in thiophene,THT,4,6DMDBT,DBT,BT and 4-MDBT,calculated using the density functional theory(DFT),are-0.159,-0.2.8,-0.2.4,-0.2.1,-0.193 and-0.188,respectively,implying that the S-M bond between the adsorption sites and thiophene is much weaker than that between the adsorption sites and THT,4,6-DMDBT or *** sulfur removal of FCC gasoline on GaAlY is 68%.

关键词： isomorphous substitution DFT model fuels adsorptive desulfurization