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Deguelin regulates nuclear pore complex proteins Nup98 and Nup88 in U937 cells in vitro1

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Acta Pharmacologica Sinica 2005年第10期26卷 1265-1273页

作者： Hong-li LIU~2. Yan CHEN~(2.3), Guo-hui CUI~2. Qiu-ling WU~2. Jing HE~2. Wei-hua CHEN~2. Jian-feng ZHOU~4 ~2.department of Hematology, Union Hospital, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 43002. ~4 department of Hematology, Tongji Hospital, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, china department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro. Methods: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. Results: The prolif eration of U937 cells was inhibited in the deguelin-treated group, with a 2.-h IC50value of 2..61 nmol/L and a 36-h IC50value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G0/G1phase, whereas cells accumulated in the S and G2.M phases. Nup88 and Nup98 were found on both the nuclar and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. Conclusion: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G2.M phases, so that the proportion of cells in the G0/G1phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.

关键词： deguelin U937 cells nucleoporin Nup98 Nup88

Study on Syntheses and Characterizations of Polymethylphenethylsilane, Polymethylcyclohexylsilane and their Copolymers

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Journal of Central South University 2000年第2期12卷 92-96页

作者： HU Hui ping 1,HUANG Ke long 1,PAN Chun yue 1,CHEN De ben 2.(1 college of chemistry and Chemical Engineering, Central South University of Technology, Changsha 410083,{2. 2.department of chemistry, Sichuan University, Chengdu 610064,{2. college of chemistry and Chemical Engineering Central South University of Technology Changsha China department of chemistry Sichuan University Chengdu China

The effects of adding cosolvents of diglyme and 15-crown-5 to the reaction mixture of Wurtz-type coupling of dichlorosilanes on the yield and relative molecular mass dispersity of polymethylphenethylsilane(PMPES) were discussed. The results show that addition of 10%(volume ratio of diglyme to toluene) diglyme as a cosolvent to the reaction mixture leads to the yield increase of PMPES with a monomodal distribution of relative molecular masses. Adding 10%diglyme to the reaction mixtures, the yields of polymethylcyclohexylsilane(PMCS) and copolymers (polymethylphenethylsilane-co-methylcyclohexylsilane), (the molar ratios of methylphenethyldichlorosilane to methylcyclohexyldichlorosilane were 2.0, 1.0 and 0.5, and the copolymers were abbreviated by Copolymers I, II, III, respectively) are 47%, 52., 54%, 53%, respectively. Their relative molecular masses ([`(M)]w )(\bar M_w ) almost reach 105. These polysilanes were characterized by 1H-NMR, IR and UV absorption spectrum.

关键词： polysilane cosolvent synthesis characterization

Distinguishing malignant from normal stomach tissues and its in vivo, in situ measurement in operating process using FTIR Fiber-Optic techniques

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Science china chemistry 2005年第2期48卷 167-175页

作者： XU Yizhuang1, YANG Limin1, XU Zhi3, ZHAO Ying2. LING Xiaofeng3, LI Qingbo1, WANG Jiansheng4, ZHANG Nengwei3, ZHANG Yuanfu1 & WU Jinguang1 1. The State Key Laboratory of Rare Earth Materials and Applications, college of chemistry and Molecular Engineering, Peking University, Beijing 100871,{2. 2. Institute of chemistry, Chinese Academy of Sciences, Beijing 100080,{2. 3. department of Surgery, Third Hospital, Peking University, Beijing 100083,{2. 4. department of Oncology Surgery, the First Hospital of Xi’an Jiaotong University, Xi’an 710061,{2. Correspondence should be addressed to Wu Jinguang (email: wujg@***) The State Key Laboratory of Rare Earth Materials and Applications College of Chemistry and Molecular Engineering Peking University Beijing China department of Surgery Third Hospital Peking University Beijing China Institute of chemistry Chinese Academy of Sciences Beijing China department of Oncology Surgery the First Hospital of Xi’an Jiaotong University Xi’an China

Gastric tissue samples were studied using mid-IR fiber-optic attenuated total reflectance (ATR) spectroscopy. FTIR spectra of 90 tissue samples from 48 patients, including 32.normal and 58 malignant tissue samples, were chosen as examples. Malignancy was usually characterized by the absence of CH and C=O bands, a weak amide II band near 1545 cm-1, a shift of the amide I band to lower wave number, a decrease in the ～1450 cm-1 peak to less than the～1400 cm-1 peak. Subtraction spectra indicate that the amide I and amide II bands of normal and malignant tissues have larger differences in peak positions and relative intensities. The statistical analysis results confirm this conclusion. The results indicate that FTIR fiber optic techniques provide important information about cancerous tissue of the stomach, which can be used to differentiate the malignant tissue from the normal tissue. Based on the above results we successfully realize the detection of the tumor tissues of digestive tract in vivo and in situ. And the results of detection cancer near operating room and in vivo and in situ in the operating room are consistent with the conclusions for the samples stored in liquid N2. which is the basis for the clinical application.

关键词： FTIR, Fiber-Optics, stomach tissues, cancer, diagnosis.

X-ray spectra induced by slow highly charged Ar^(q+) ions in collision with Nb surface

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Science china(Physics,Mechanics & Astronomy) 2008年第10期51卷 1463-1469页

作者： YANG ZhiHu1, CUI Ying2. CHEN XiMeng2. SONG ZhangYong1, SHAO JianXiong2. RUAN FangFang1, ZHANG HongQiang2. DU Juan2. LIU YuWen2. GAO ZhiMing2. ZHANG XiaoAn3, ZHU KeXin1, YU DeYang1 & CAI XiaoHong1 1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, china 2.department of Modern Physics, Lanzhou University, Lanzhou 730000, china 3 department of Physics, Xianyang Normal college, Xianyang 712.00, china Institute of Modern Physics Chinese Academy of Sciences Lanzhou China department of Modern Physics Lanzhou University Lanzhou China department of Physics Xianyang Normal College Xianyang China

The X-ray spectra of Nb surface induced by Arq+ (q =16,17) ions with the energy range from 10 to 2. keV/q were studied by the optical spectrum technology. The experimental results indicate that the multi-electron excitation occurred as a highly charged Ar16+ ion was neutralized below the metal surface. The K shell electron of Ar16+ was excited and then de-excited cascadly to emit K X-ray. The intensity of the X-ray emitted from K shell of the hollow Ar atom decreased with the increase of projectile kinetic energy. The intensity of the X-ray emitted from L shell of the target atom Nb increased with the increase of projectile kinetic energy. The X-ray yield of Ar17+ is three magnitude orders larger than that of Ar16+.

关键词： highly charged ion hollow atom multi-electron X-ray

Complex variable method for plane elasticity of icosahedral quasicrystals and elliptic notch problem

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Science china(Physics,Mechanics & Astronomy) 2008年第7期51卷 773-780页

作者： LI LianHe1,2.& FAN TianYou1 1 department of Physics, Beijing Institute of Technology, Beijing 100081, china 2.{2. of Mathematics Science, Inner Mongolia Normal University, Hohhot 01002., china department of Physics Beijing Institute of Technology Beijing China college of Mathematics Science Inner Mongolia Normal University Hohhot China

The complex variable method for the plane elasticity theory of icosahedral quasicrystals is developed. Based on the general solution obtained previously, complex representations of stress and displacement components of phonon and phason fields in the quasicrystals are given. With the help of conformal transformation, an analytic solution for the elliptic notch problem of the material is presented. The solution of the Griffith crack problem can be observed as a special case of the results. The stress intensity factor and energy release rate of the crack are also obtained.

关键词： icosahedral quasicrystals plane elasticity elliptic notch complex variable method

Gastroesophageal manometry and 2.-hour double pH monitoring in neonates with birth asphyxia

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World Journal of Gastroenterology 2001年第5期7卷 695-697页

作者： Mei Sun~1 Wei-Lin Wang~2.Wei Wang~2.De-Liang Wen~1 Hui Zhang~1 Yu-Kun Han~1 1 Pediatric department,2.Pediatric Surgery department,Second Clinical {2.,china Medical University,Shenyang 110003,china Pediatric department Second Clinical College China Medical University Shenyang 110003 China. meis@***

INTRODUCTIONBirth asphyxia may lead to disturbances of gastroenteric motility of newborn infants[1.2. . The change of gut pressure and reflux are the major manifestations of the motor disturbance [3-9] . To evaluate the effects of perinatal asphyxia on the gastroenteric motility, gastric and esophageal pressure and double pH were measured in a group of asphyxiated newborns. And. their pathophysiological and anatomical effects on gastroenteric function were discussed.

关键词： Asphyxia Neonatorum Esophagus Gastroesophageal Reflux Humans Hydrogen-Ion Concentration Infant, Newborn Manometry Monitoring, Physiologic Research Support, Non-U.S. Gov't Stomach

Effects of extract F of red-rooted Salvia on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock-reperfusion in rats

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World Journal of Gastroenterology 2001年第5期7卷 672-677页

作者： Li-Hong Zhang~1 Chang-Bai Yao~2.He-Quan Li~3 1 department of Anesthesiology,Second Clinical college,{2. Medical University,Shenyang 110003,Liaoning Province,{2.2.department of General Surgery,Second Clinical college,{2. Medical University3 department of Pathophysiology,{2. medical University department of Anesthesiology Second Clinical College China Medical University Liaoning Province China. cumzhanglihong@***

AIM: To compare the effects of extract F of red-rooted Salvia (EFRRS) on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock and reperfusion in rats. METHODS: The rats were subject to hemorrhagic shock and followed by reperfusion, and were divided randomly into two groups. Group 1 received saline, and group 2.received EFRRS intravenously. The index of gastric mucosal lesions (IGML) was expressed as the percentage of lesional area in the corpus or antrum. The degree of gastric mucosal lesions (DGML) was catalogued grade 0,1,2.and 3. The concentrations of prostaglandins (PGs) were measured by radioimmunoassay. The concentration of MDA was measured according to the procedures of Asakawa. The activity of SOD was measured by the biochemical way. The growth rates or inhibitory rates of above-mentioned parameters were calculated. RESULTS: As compared with IGML (%), grade 3 damage (%) and MDA content (nmol/g tissue) of gastric antrum which were respectively 7.96 +/- 0.59, 34.86 +/- 4.96 and 156.98 +/- 16.12. those of gastric corpus which were respectively 2..18 +/- 6.82. 58.44 +/- 9.07 and 2.0.56 +/- 19.37 increased markedly (P 2. and PGI(2.(pg/mg tissue), the ratio of PGI(2./TXA(2. and the activity of SOD (U/g tissue) of corpus which were respectively 3.01 +/- 1.01, 8.35 +/- 1.95, 540.48 +/- 182.78, 714.38 +/- 12..74, 17.38 +/- 5.93 and 134.2. +/- 13.35 were markedly lower than those of antrum which were respectively 13.92.+/- 2.2., 2..78 +/- 6.06, 2.18.56 +/- 433.12. 2.31.76 +/- 492.35, 43.46 +/- 8.51 and 187.45 +/- 17.67 (P2. and PGI(2., the ratio of PGI(2./TXA(2. and the activity of SOD of corpus which were respectively 632.56, 308.62. 40.75, 74.75, 92.2. and 12..2. were higher than those in antrum which were respe

关键词： Phytotherapy Salvia Alprostadil derivatives Animals Dinoprostone Gastric Mucosa Hydroxyl Radical Male Malondialdehyde Plant Extracts Pyloric Antrum Rats Rats, Wistar Reperfusion Injury Research Support, Non-U.S. Gov't Shock, Hemorrhagic Superoxide Dismutase Thromboxane B2

Autoantibody against Cardiac β1-Adrenoceptor Induces Apoptosis in Cultured Neonatal Rat Cardiomyocytes

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 443-449页

作者： Yan GAO~1 Hui-Rong LIU~1 Rong-Rui ZHAO~1 Jian-Ming ZHI~(2.) 1 department of Cardiovascular Physiology Shanxi Medical University,Taiyuan 030001,china 2.department of Physiology,Shanghai Jiaotong University,college of Basic Medicine,Shanghai 2.002.,china department of Cardiovascular Physiology Shanxi Medical UniversityTaiyuan 030001 China department of Physiology Shanghai Jiaotong University College of Basic MedicineShanghai 200025 China

To clarify whether apoptosis is involved in the injury processes induced by autoantibodyagainst cardiac β1-adrenoceptor,we investigated the biological and apoptotic effects of antibodies on culturedneonatal rat *** rats were immunized with peptides corresponding to the second extra-cellular loop of the β1-adrenoceptor to induce the production of anti-β1-adrenoceptor antibodies in the ***（Ig）G in the sera was detected using synthetic antigen enzyme-linked immunosorbentassay and purified using the diethylaminoethyl cellulose ion exchange *** of cardiomyo-cytes was evaluated using agarose gel electrophoresis and flow *** results showed that thepositive serum IgG greatly increased the beating rates of cardiomyocytes and showed an"agonist-like"***,positive serum IgG induced cardiomyocyte apoptosis after treatment with β1-adrenoceptor overstimulation for *** effects of monoclonal antibody against β-adrenoceptor werealso found to be similar to those of positive serum *** was suggested that the autoantibody could inducecardiomyocyte apoptosis by excessive stimulation of β1-adrenoceptor.

关键词： β1-adrenoceptor rat cardiomyocyte autoantibody apoptosis

Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin F2.

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Acta Pharmacologica Sinica 2007年第8期28卷 1149-1154页

作者： Qing-song JIANG~2.Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2.6) ~2.0} of Pharmacology,Chongqing Medical University,Chongqing 400016,china ~3department of Pharmacology,Zunyi Medical college,Zunyi 563003,china ~4department of Biochemistry Zunyi Medical college,Zunyi 563003,china ~5Chengdu Vocational college of Agricultural Science and Technology,Chengdu 611100,china department of Pharmacology Chongqing Medical University Chongqing China department of Pharmacology Zunyi Medical College Zunyi China department of Biochemistry Zunyi Medical College Zunyi China Chengdu Vocational college of Agricultural Science and Technology Chengdu China

Aim:To examine the antihypertrophic effect of ginsenoside Rb1（Rb1）induced byprostaglandin F2.(PGF2.)in vitro and to investigate the possible mechanismsinvolved in the calcineurin（CAN）signal transduction ***:Thecardiomyocyte hypertrophy induced by PGF2.and the antihypertrophic effect ofRbiwere evaluated in primary culture by measuring the cell diameter,proteincontent,and atrial natriuretic peptide（ANP）mRNA *** and CaNmRNA expressions,CaN and its downstream effectors NFAT3and GATA4 proteinexpressions,and the intracellular free Ca2.concentration([Ca2.]i)were assayedby RT-PCR,Western blot,and fluorescent determination using Fura 2.AM,***:PGF2.（100 nmol/L）significantly increased the cardiomyo-cyte diameter,protein content and[Ca2.]i,and promoted ANP,CaN mRNA,andCaN/NFAT3/GATA4protein expressions,which were inhibited by either Rb1in aconcentration-dependent manner（50,100,and 2.0μg/mL）or L-arginine（1 mmol/L）.NG-nitro-L-arginine-methyl ester,a nitric oxide synthase inhibitor,could abolishthe effects of L-arginine,but failed to change the effects of Rb1in the ***:The present data implicate that Rb1attenuates cardiachypertrophy,the underlying mechanism may be involved in the inhibition of theCa2.-CaN signal transduction pathway.

关键词： Rb1 prostaglandin F2. cardiac hypertrophy calcineurin