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中国化学会第六届全国结构化学学术会议

作者： Bo Xu~(,*),Guang-Ning Liu,Qi-Sheng Liu,Cun-Cheng Li college of chemistry and Chemical Engineering,{2. of Jinan,Jiwei Road 106,Jinan, 2.002.

The designed synthesis of new coordination polymers with interesting topological structures has attracted great attention in recent *** have long been focusing on the research of entangled networks based on isophthalic acids and bipyridyl *** we report a new coordination polymer formulated as[Zn(mip)(bpe)]·HO(1)(Hmip = 5-methylisophthalic acid,bpe = 1,2. bis-(4-pyridyl) ethane).X-ray diffraction analyses show that 1 holds a 3D three-fold interpenetrating network based on dmp units.

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Isolation of a cellulase-producing bacterium,cloning and expression of its novel β-glucosidase gene

Isolation of a cellulase-producing bacterium,cloning and exp...

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重庆微生物学会第九届会员代表大会暨学术年会

作者： Ma Zhengang~1 Tang Jing~1 Lan Xiqian~1 Xiao Jie~1 Pan Guoqing~1 Zhou Zeyang~(1,2.) (The key Sericultural Laboratory of Agricultural Ministry.Southwest university.Chongqing 400716,china) ({2. of Bioscience of Chongqing Normal university.Chongqing 40047,china)

[Objective]In order to get a cellulase-high-yielding bacterium,and try to obtain some novel cellulase genes.[Methods]CMCNa plate screening method was used to screen cellulose-producing bacteria strains,and one obtained strain was identified according to its 16S rDNA sequence,physiological and biochemical ***,a gene encoding a novel cellulolytic enzyme was cloned from this strain, and its sequence characters were ***,the heterologous expression of this gene was tested in a prokaryotic expression system.[Results]A facultative anaerobic strain which produced cellulase was obtained by the CMCNa plate *** strain was identified as Klebsiella sp..By a shotgun cloning method and with Escherichia coli as the host cell,we isolated a novelβ-glucosidase gene,nglu02.from the *** nglu02.was then successfully expressed in transformant *** strain BL2.,and the recombinant enzyme was partially characterized.β-glucosidase activity could be detected in the supernatant of the transformant ***’s worth noting that the recombinant enzyme had certain alkali resistance and could keep its activity with the exist of Cu,Fe,EDTA and urea.[Conclusion]We successfully cloned a novelβ-glucosidase gene,nglu02.from the cellulase-producing *** also expressed the gene in prokaryotic expression system and testified that the produced protein was *** study offered some useful information for the endeavor to understand the molecular structure and mechanism of cellulolytic enzymes.

关键词： Cellulase Novelβ-Glucosidase Screen Identification Activity

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Expression of a Hybrid Human Superoxide Dismutase with a High Affinity for Heparin

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Chemical Research in Chinese Universities 2002年第1期18卷 20-24页

作者： GOU Xiao-jun, YIN Shao-man, KONG Xiang-duo, ZHU Shi-zhen WANG Xiao-ping, ZHANG Hong-ying and ZHANG Jin Key Laboratory for molecular Enzymology and Engineering of Ministry of Education, Jilin university, Changchun 13002., P. R.{2.ZHU HongThe Jilin Provi Jilin university Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Changchun 130023 The Jilin Province People's Hospital Changchun 130031 college of chemistry Jilin University Changchun 130023

A designed heparin-affinity of human Cu, Zn-SOD is described. The natural leader peptide of *** Cu, Zn-SOD and a heparin-binding peptide containing a stretch of 7 Arg were fused to the N-terminal and the C-terminal of human Cu, Zn-SOD respectively. The resulted hybrid enzyme had not only a normal SOD activity but also a high affinity for heparin eluted on the heparin-Sepharose column at 0.4 mol/L NaCl. Some properties, such as the optimum pH, the thermostability and the half-life in the circulation of rats, were also analyzed.

关键词： Human superoxide dismutase Heparin affinity Gene expression

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molecular and morphological evidence indicate that Pseudorhabdosynochus lantauensis (Monogenea:Diplectanidae) represents two species

Molecular and morphological evidence indicate that Pseudorha...

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中国畜牧兽医学会家畜寄生虫学分会第五次代表大会暨第八次学术研讨会

作者： X.Y.WU~1 N.B.CHILTON~2.X.Q.ZHU~3 M.Q.XIE~1 A.X.LI~(1*) 1Center for Parasitic Organisms,School of Life sciences,Sun Yat-sen university,135 Xingang West Street,Haizhu District,Guangzhou 5102.5,Guangdong Province,The People’s Republic of{2. 2.epartment of Biology,university of Saskatchewan,112.Science Place,Saskatoon,SK,STN 5E2.Canada 3Laboratory of Parasitology,college of Veterinary Medicine,South{2. Agricultural university,Guangzhou 510642.Guangdong Province,The People’s Republic of{2.

Sequences of the first internal transcribed spacer(ITS-1)and the D1-D3 domains of the largesubunit(LSU)of the ribosomal DNA(rDNA)were determined for multiple specimens of fouroperational taxonomic units(OTUs)of the monogenean,Pseudorhabdosynchous *** defined based on their collecting localities,host and/or morphological *** *** specimens of one group(OTUs 1 and 3)differed in their sequences of the ITS-1 and partialLSU rDNA when compared with specimens of a second group(OTUs 2.and 4)by 12. and 2.,*** of the phylogenetic analyses of the LSU rDNA sequence data showed total(100%)bootstrap support for the separation of *** into two distinct *** least 11 of the 18nucleotide differences in the LSU sequence between the two *** elades were derived(***)characters when the morphologically distinct species,*** and ***,were used as ***,there were several ftxed autapomorphic character states for each *** *** provides sufficient evidence to reject the null hypothesis that *** a single *** and morphometric differences between these two cladesprovided additional strong support for the separation of *** into two *** twoparasite species were found to co-exist in one of the two species of serranid fish(***)examined in the South china Sea(Guangdong Province,china).

关键词： Pseudorhabdosynchous lantauensis first internal transcribed spacer large subunit rDNA DNA Sequencing Monogenea Epinephelus

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Cloning and Sequence Analysis of EF1αGene of the Giardia lamblia from Dogs in Guangzhou

Cloning and Sequence Analysis of EF1αGene of the Giardia la...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Li Jie,Wang Peiyuan,Zhang Ping,Liu Yuanjia,Guo Jianchao,Meng XiangIong,Li GuoQing~* college of Veterinary Medicine,South china Agricultural {2.,Guangzhou 510642.china

In order to amplify the EF1αgene of Giardia lamblia isolated from dogs in Guangzhou,one pair of primers EF/ER were designed based on the published sequence of the EF1αgene in GenBank and the amplicons were cloned into pGEM-T Easy vector *** recombinant plasmids were identified by PCR and the products were sequenced and *** results show that the EF1αof the dog-derived Giardia strain is 2.8 bp in *** phylogenetic tree revealed that the dog-derived Giardia strain was Giardia lamblia Assemblage *** results provided a foundation for further studies of molecular identification and molecular genetics of Giardia lamblia.

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用两种方法合成长余辉发光玻璃的对比研究

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中国稀土学报 2002年第6期20卷 625-629页

作者：朱爱玲廉世勋李承志毛向辉湖南师范大学化学化工学院长沙410081

在还原性气氛下高温固相反应法(一步法)合成了长余辉发光玻璃GA;用SrAl2.4:Eu,Dy荧光粉(SAED)与玻璃粉混合高温熔融(二步法)合成了长余辉发光玻璃GB和GC。通过XRD、发光光谱和磷光衰减曲线对样品进行了表征。荧光粉SAED和玻璃GA,GB,GC... 详细信息

在还原性气氛下高温固相反应法(一步法)合成了长余辉发光玻璃GA;用SrAl2.4:Eu,Dy荧光粉(SAED)与玻璃粉混合高温熔融(二步法)合成了长余辉发光玻璃GB和GC。通过XRD、发光光谱和磷光衰减曲线对样品进行了表征。荧光粉SAED和玻璃GA,GB,GC的发射峰值依次是52.,493,462.516nm,发光强度和余辉时间都是SAED>GC>GB>GA。玻璃的发射光谱峰随玻璃组成不同发生位移。两种方法合成的夜光玻璃发光性质存在着明显的差异。同一合成方法下,玻璃基料决定着玻璃网络结构,同时也影响着夜光玻璃的发光性质。

关键词：稀土长余辉发光玻璃合成固相反应高温熔融发光性质

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Facile Three-component Synthesis of Spirooxindoiepyrroloiine Ring Systems via 1,3-dipolar Cycloaddition with 1,4-Naphthoquinone

Facile Three-component Synthesis of Spirooxindoiepyrroloiine...

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中国化学会第四届有机化学学术会议

作者： WANG, Shun-Yi LIU, Bing JI, Shun-Jun (Key Laboratory of Organic Synthesis of Jiangsu Province, college of chemistry and Chemical Engineering, Suzhou {2., Suzhou 2.5006)

＜正＞The 1,3-dipolar cycloaddition reactions have attracted continuous attention as an efficient method to the preparation of heterocycles and natural products. With that, 1,3-dipolar cycloadditions involving 1,4-naphthoquinone and an azomethine ylide prepared from α-amino acids and isatins, which have been used in this reaction to afford the pyrrolidine-2.spiro-3’-oxindole 4 with

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molecular Diversity and Differential Expression of Starch-Synthesis Genes in Developing Kernels of three Maize Inbreds

Molecular Diversity and Differential Expression of Starch-Sy...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Xiang-Zhen Ding1,2., Bei-Guo Wang1*, Qing-Hua Gao3, Qiong Zhang1, Gui-Qin Yan2. Ke Duan1*, Jian-Hua Huang1* 1Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Biological Technique, Shanghai Academy of Agricultural sciences (SAAS), Shanghai, 2.1106, china 2.2. of Life sciences, Shanxi Normal university, Linfen, 041104, china 3Shanghai Key Laboratory of Protected Horticultural Technology, Forest & Fruit Research Institute of SAAS, Shanghai, 2.1106, china.

＜正＞Maize （Zea mays L.） is one of the most important agricultural crops in the grass family, and also is a source of tremendous phenotypic and molecular diversity[1,2.. Starch composition （the ratio of amylose/am... 详细信息

关键词： Zea mays starch-synthesis gene expression pattern nucleic polymorphism mis-splicing event mutation

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