检索结果-南通市图书馆

Expression and Purification of the DNA Binding Domain of the Epstein-Barr Virus Nuclear Antigen 1

在线全文

学校读者我要写书评

暂无评论

生物技术通报 2008年第S1期24卷 405-410页

作者： Meiru Hu~1 Ru Wei~(1,2.Lu Qian~1 Ming Yu~1 Ming Shi~1 Kun He~3 Jie Wang~3 Beifen Shen~1 Ning Guo~1 (1 Institute of Basic Medical Sciences,beijing 100850 2.North china Coal Medical College 3 National {2. of biomedical Analysis,beijing 100071)

To overexpress EBNA-1 in *** and generate the specific antibody,in this study,the antigenicity,epitope and hydrolysis of EBNA-1 were analyzed using the computer design software *** on the prediction by computer analysis,the sequence encoding EBNA-1385-557 was amplified by PCR with the specific *** expression vector containing EBNA-1385-557 coding sequence was ***-tagged EBNA-1385-557 was expressed in *** soluble recombinant protein was purified using Ni-NTA *** purified protein was used as antigens to immunize mice and screen the antibodies,which will serve as an important tool for further studies.

关键词： EBNA-1 Expression Purification E.coli

在线全文

学校读者我要写书评

暂无评论

基于GIS的流量倾向线的自动绘制与研究

计算机工程与应用 2004年第3期40卷 185-187页

作者：奚宽武石建军北京工业大学交通研究中心北京100022

该文首先介绍了道路规划中的OD矩阵合并技术,而后运用地理信息系统技术的二次开发实现了OD流量倾向线的自动绘制,提高了道路规划中流量倾向线的可视化、直观化及可操作化,并给出一些实例分析。

关键词：地理信息系统流量倾向线道路规划 GIS 自动绘制

Molecular biology evaluation of Polyacrylamide Hydrogel using real time PCR

在线全文

学校读者我要写书评

暂无评论

Molecular biology evaluation of Polyacrylamide Hydrogel usin...

Synthesis,Characterization, and Antibacterial Activity of Quaternized of N-aromatic Chitosan Derivatives

第2.世界华人生物医学工程研讨会

作者： Chengxiang Fan1 Tingfei Xi1 a Ziyi Wan2.Xixiong Kang3 Yajie Wang3 Chunren Wang1 1. center for Medical Devices National Institute for the Control of Pharmaceutical & Biological Products beijing 100050 P.R.{2. 2. School of Materials Science and engineering beijing Institute of Technology beijing 100081 P.R.{2. 3. Titan Hospital Capital univer.ity of Medical Science beijing 100050 P.R.{2. a

Polyacrylamide Hydrogel, which is widely used in ophthalmic operations, drug treatment, food packaging products, and water purification etc, is a watery gel consisting of approximately 2.5% cross-lin

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Synthesis,Characterization, and Antibacterial Activity of Qu...

STUDY OF DIELECTRIC PROPERTIES OF HUMANRED BLOOD CELL SUSPENSION FROM 2.3HEALTHY PERSONS AT RADIO FREQUENCIES

2.10 3rd International Conference on Future biomedical Information engineering (FBIE 2.10)

作者： Han Yongping Bio-chemical engineering college of beijing union {2. 10002. china Lin Qiang Bio-chemical engineering college of beijing union {2. 10002. china

The quaternized of N-aryl chitosan （CTS） derivatives containing different aromatic moieties were synthesized by two steps, N-aromatic Schiff and the quaternization. The chemical structures of all chitosan derivatives, N- vanillin Schiff’s base chitosan （NVCh）, N- cinnamaldehyde Schiff’s base chitosan （NCCh）, N- benzaldehyde Schiff’s base CTS （NBCh）, and their quaternized of N- aromatic CTS derivatives were characterized by ATR-FTIR. The water solubility of the N-aromatic Schiff’s base CTS derivatives had very poor water solubility. But after quaternized, the water solubility of CTS derivatives was obviously improved. The antibacterial studies of these CTS derivatives were carried out by the inhibition zone diameters methods against *** （Gram-negative）, *** and *** （Gram-positive） bacteria, and against *** niger （fungal）. It was found that the quaternized NVSCTS showed higher antibacterial activity than quaternized NCCh and quaternized NVBCh at the almost same DQ and ES level. In comparison to each of the chemical structure, it was found that the phenol hydroxyl group effect on antibacterial activity was even higher than that of positive charge density of CTS.

关键词： chitosan Antibacterial activity Quaternized N- aromatic chitosan derivatives

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Journal of biomedical engineering(English Edition) 1994年第1期3卷 20-27页

作者： Lu Yongjun,Yu Jue,Ren Yinhua(Dept. BME, Institute of Basic Medical Sciences, beijing, 100005,china)Zhang Zhixin,Sun Yanmei,Zhi Hong,Yuan Qinzhen(Blood {2. of beijing Red Cross Society,beijing, 100086,china)

Dielectric properties or kuamn red blood cell (RBC) suspension (ltematocrit 50%) from 2.3 healthy persons (12. males, 12. remales) were measured at 2.Cin the frequency range rrom 1 to 500MHz, and analysed statistically. The measuringsystem controlled by an IBM-PC computer was composed or a network analyzer(HP4195A), coaxial line sensor and a temperature controlling set. The results showedthat the dielectric properties did not depend on either sex or blood group. However, agehad definite erfect. There was a critical age around 49 years when the dielectric data ofhuman RBC suspensions varied signiricantly.

关键词： Human Red Blood Cell Suspension Cell Suspension Dielectric properties Heaalthy volunteer Biodielectric Measurement

The Weekly Characteristics of Adolescent Behavioral Autononmy And the Relationship betweem Behavioral Autonomy and Attachment

在线全文

学校读者我要写书评

暂无评论

The Weekly Characteristics of Adolescent Behavioral Autononm...

Prestin forms tetramer with each subunit being mechanically independent

2.10 IEEE 2.d Symposium on Web Society

作者： Ting Hu~(a,*),Na Wen~b a,Mental health Education and Mental Counseling center,Guangzhou City {2.,china b,Department of Psychology,Capital Normal univer.ity,beijing,china

＜正＞To attain autonomy is an important task for adolescent,as well as a key to adolescent normal psychological social *** the same time, the harmonious relationship with parents is good for the heathy development of adolescent behavioral autonomy,and also promote adolescent social *** explore the weekly characteristics of adolescent behavioral autonomy and the relationship between behavioral autonomy and adolescent-parents attachment,this research used the methord of day-level measures,154 junior high school students as participants provided daily reports for 14 days on behavioral autonomy and the Inventory of Parent and Peer *** result indicated that the fluctuate trend of junior high school students’ daily behavioral autonomy mainly embodying in ***, the relationship with parents also has effect on their behavioral autonom,,the autonomy of adolsecents with secure attachment is significant higher than insecure attachmen,especially the relationship with fathers.

关键词： Adolescent Behavioral Autonomy Attachment

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Otology 2009年第2期4卷 86-97页

作者： WANG Xiang 1, GUO Wei-wei 2. HE David Zhi-Zhou1, YANG Shi-Ming 2.1 Department of biomedical Sciences, Creighton univer.ity School of Medicine, Omaha, NE 68178, USA 2.Institute of Otolaryngology, Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, {2. 100853 Department of biomedical Sciences Creighton University School of Medicine Omaha NE 68178 USA Institute of Otolaryngology Department of Otolaryngology Head and Neck Surgery Chinese PLA General Hospital Beijing 100853

Prestin is the motor protein of cochlear outer hair cells （OHCs）. It is able to perform rapid and reciprocal electromechanical conversion that underlies OHC electromotility. Due to the inadequate size of a single prestin molecule to form the2.nm intramembraneous protein particles （IMPs） in the OHC lateral membrane （LM）, the possibility of prestin oligomerization has been proposed. It has been suggested that prestin molecules form highorder oligomers, most likely as the tetramer, in heterologous systems. In OHCs, however, the oligomeric structure of prestin remains unclear. Here we calculated the prestin-related charge density in both gerbil and guinea pig OHCs through measuring their nonlinear capacitance （NLC） and LM surface area, showing that the average charge density （2., 608 μm-2.in gerbils; 19, 460 μm-2.in guinea pigs） is statistically 4 times the average density of IMPs （5,686 μm-2.in gerbils; 5, 000 μm-2.in guinea pigs）. This suggests that each IMP contains four prestin molecules based upon the notion that each prestin transfers a single elementary charge, implying that prestin forms tetramers in OHCs. To determine whether the prestin tetramer functions as a mechanical unit, we subsequently compared the slope factors （α） of electromotility and NLC simultaneously measured from the same OHC, showing that the α values of the two are statistically the same. This suggests that each prestin molecule in the tetramer is mechanically independent and equally contributes to OHC electromotility.

关键词： Prestin oligomer outer hair cells electromotility nonlinear capacitance gerbil

980nm InGaAs应变量子阱激光器及组合件

在线全文

学校读者我要写书评

暂无评论

中国激光 1997年第10期24卷 873-876页

作者：徐遵图徐俊英杨国文张敬明肖建伟陈良惠沈光地中国科学院半导体所国家光电子工程研究中心北京100083 北京工业大学光电子技术实验室北京100022

利用分子束外延技术研制出了高质量InGsAs/GaAs应变量子阱材料及量子阱激光器.脊形波导窄条形量子阱激光器的阈值电流和微分量子效率分别为15mA和0.8 W/A,线性输出功率大于150mW,基横模输出功率可达***应变量子阱激光器和单模光纤进行... 详细信息

利用分子束外延技术研制出了高质量InGsAs/GaAs应变量子阱材料及量子阱激光器.脊形波导窄条形量子阱激光器的阈值电流和微分量子效率分别为15mA和0.8 W/A,线性输出功率大于150mW,基横模输出功率可达***应变量子阱激光器和单模光纤进行了耦合,其组合件出纤光功率典型值为40mW,最大值可达60mW.显示出了高的基横模输出功率和高的耦合效率.其组合件在40～60mW下,中心发射波长在977nm.满足了对掺铒光纤高效率泵浦的波长要求,成功地研制出适于掺铒光纤放大器用的应变量子阱激光器.

关键词：应变层量子阱半导体激光器

在线全文

学校读者我要写书评

暂无评论

基于迭代自学习的操纵子结构预测

生物化学与生物物理进展 2011年第7期38卷 642-651页

作者：吴文琪郑晓斌刘永初汤凯朱怀球北京大学湍流与复杂系统国家重点实验室工学院生物医学工程系理论生物中心蛋白质科学中心北京100871

原核生物操纵子结构的准确注释对基因功能和基因调控网络的研究具有重要意义,通过生物信息学方法计算预测是当前基因组操纵子结构注释的最主要来源.当前的预测算法大都需要实验确认的操纵子作为训练集,但实验确认的操纵子数据的缺乏一... 详细信息

原核生物操纵子结构的准确注释对基因功能和基因调控网络的研究具有重要意义,通过生物信息学方法计算预测是当前基因组操纵子结构注释的最主要来源.当前的预测算法大都需要实验确认的操纵子作为训练集,但实验确认的操纵子数据的缺乏一直成为发展算法的瓶颈.基于对操纵子结构的认识,从基因间距离、转录翻译相关的调控信号以及COG功能注释等特征出发,建立了描述操纵子复杂结构的概率模型,并提出了不依赖于特定物种操纵子数据作为训练集的迭代自学习算法.通过对实验验证的操纵子数据集的测试比较,结果表明算法对于预测操纵子结构非常有效.在不依赖于任何已知操纵子信息的情况下,算法在总体预测水平上超过了目前最好的操纵子预测方法,而且这种自学习的预测算法要优于依赖特定物种进行训练的算法.这些特点使得该算法能够适用于新测序的物种,有别于当前常用的操纵子预测方法.对细菌和古细菌的基因组进行大规模比较分析,进一步提高了对基因组操纵子结构的普遍特征和物种特异性的认识.

关键词：基因组分析操纵子迭代自学习预测评价