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Cloning of UGT1A9 cDNA from liver tissues and its expression in CHL cells

World Journal of Gastroenterology 2001年第6期7卷 841-845页

作者： Xin Li~(1,2. Ying-Nian Yu~1 Ge-Jian Zhu~1 Yu-Li Qian~1 1 Department of Pathophysiology,school of Medicine,Zhejiang university,{2.,china2.College of Pharmcy,Zhejiang university,{2.,china Department of Pathophysiology School of Medicine Zhejiang University Hangzhou China.

AIM: To clone the cDNA of UGT1A9 from a Chinese human liver and establish the Chinese hamster lung (CHL) cell line expressing human UGT1A9. METHODS: cDNA of UGT1 A9 was transcripted from mRNA by reverse transcriptase-ploymerase chain reaction, and was cloned into the pGEM-T vector which was amplified in the host bacteric *** DH5(alpha). The inserted fragment, verified by DNA sequencing, was subcloned into the Hind III /Not I site of a mammalian expression vector pREP9 to construct the plasmid termed pREP9-UGT1A9. CHL cells were transfected with the resultant recombinants, pREP9-UGT1A9, and selected by G418 (400 mg x L(-1)) for one month. The surviving clone (CHL-UGT1A9) was harvested as a pool and sub-cultured in medium containing G418 to obtain samples forUGT1A9 assays. The enzyme activity of CHL-UGT1A9 towards propranolol in S9 protein of the cell was determined by HPLC. RESULTS: The sequence of the cDNA segment cloned, which was 1666 bp in length, was identical to that released by Gene Bank (GenBank accession number: AF056188) in coding region. The recombinant constructed, pREP9-UGT1A9, contains the entire coding region, along with 18 bp of the 5' and 55 bp of the 3' untranslated region of theUGT1A9 cDNA, respectively. The cell lines established expressed the protein of UGT1A9, and the enzyme activity towards propranolol in S9 protein was found to be 101+/- 2. pmol x min(-1) x mg(-1) protein (n=3), but was not detectable in parental CHL cells. CONCLUSION: The cDNA of UGT1A9 was successfully cloned from a Chinese human liver and transfected into CHL cells. The CHL-UGT1 A9 cell lines established efficiently expressed the protein ofUGT1A9 for the further enzyme study of drug glucuronidation.

关键词： Cloning, Molecular Gene Expression Animals Cell Line Cricetinae Cricetulus DNA, Complementary Glucuronosyltransferase Humans Liver Molecular Sequence Data Research Support, Non-U.S. Gov't

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A new method for adaptive color image filtering

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Chinese science Bulletin 2000年第13期45卷 1230-1235页

作者： JIANG Gangyi, YU Mei & YU Bokang1. Department, of Information & Electronic Engineering, Hang/hou university of Commerce, hangzhou 310035, {2. 2. Department, of Electronic Engineering, hangzhou institute of Electronics Engineering, hangzhou 310037, {2. 3. State Key Laboratory on Machine Perception, Peking university, Beijing 100871, {2. Correspondence should be addressed to Yu Mei Department of Information & Electronic Engineering Hangzhou University of Commerce Hangzhou China State Key Laboratory on Machine Perception Peking University Beijing China Department of Electronic Engineering Hangzhou Institute of Electronics Engineering Hangzhou China

An adaptive color image filter (ACIF) is proposed in this note. Through analyzing noise corruption of color image, efficient locally adaptive filters are chosen for image enhancement. The proposed adaptive color image filter combines advantages of both nonlinear vector filters and linear filters, it attenuates noise and preserves edges and details very well. Experimental results show that the proposed filter performs better than vector median filter, directional-distance filter, directional-magnitude vector filter, adaptive nearest-neighbor filter, and α-trimmed mean filter.

关键词： color image noise analysis locally adaptive filters adaptive color image filter (ACIF).

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Inhibitory effect of acetamide-45 on airway inflammation and phosphodiesterase 4 in allergic rats

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Acta Pharmacologica Sinica 2005年第12期26卷 1492-1496页

作者： Kai WANG~2.Hua-hao SHEN~(2.4) Jun-chun CHEN~2.Zhong CHEN~3 2.Respiratory Department,the Second Affiliated Hospital,Medical school of Zhejiang university,{2. 310009 3 Department of Pharmacology,Medical school of Zhejiang university,{2. 310031,china Respiratory Department the Second Affiliated Hospital Medical School of Zhejiang University Hangzhou China Department of Pharmacology Medical School of Zhejiang University Hangzhou China

Aim:To determine the effects of acetamide-45 on respiratory function,airwayinflammation,and the activity of phosphodiesterase 4（PDE4）in allergic ***:Rats were sensitized by a single intramuscular injection with ovalbumin（OVA）and were challenged with ovalbumin applied by using an aerosol repeat-edly for 7 d after 2.***-45 at concentrations of 5,10,or 30 mg/kg wasthen administered by intraperitoneal *** in dynamic lung compli-ance and lung resistance,the accumulation of inflammatory cells in bronchoalveolarlavage,PDE4 activity,and the concentration of interleukin-4 in rat lung tissuewere ***:Seven days of treatment with acetamide-45 preventedeosinophil accumulation in allergic *** doses of 5,10,and 30 mg/kg,aceta-mide-45 decreased lung resistance to 0.2.±0.04,0.2.±0.07,and 0.2.±0.05cmH2.·s-1·mL-1,respectively（P2.0.09 mL/cmH2.（P2.1±55,2.3±57,and 2.8±36 nmol·g-1·min-1（P2.±1.13,5.95±1.2.,and 5.68±2.2. μtg/g protein（P<0.05 vs OVA）.Conclusions:Acetamide-45 wasfound to improve respiratory function and inhibit airway inflammation in thisanimal model,and the PDE4 activity of lung tissue was obviously ***-45 was an effective anti-inflammatory agent in respiratory inflammation,and the mechanism of its action might depend on inhibition of PDE4.

关键词： acetamide-45 3’,5’-cyclic nucleotide phosphodiesterase respiratory function tests eosinophils asthma

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Ordinal scheduling problem and its asymptotically optimal algorithms on parallel machine system

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science in china(Series F) 2004年第2期47卷 161-169页

作者： TAN Zhiyi1,2.& HE Yong1 1. Department of Mathematics, Zhejiang university, hangzhou 31002., {2. (email: tanzy@math.zju. *** heyong@math.zju.***) 2. State Key Laboratory of CAD & CG, Zhejiang university, hangzhou 31002., {2. Department of Mathematics Zhejiang University Hangzhou China State Key Laboratory of CAD & CG Zhejiang University Hangzhou China

Focusing on the ordinal scheduling problem on a parallel machine system, we discuss the background of ordinal scheduling and the motivation of ordinal algorithms. In addition, for the ordinal scheduling problem on identical parallel machines with the objective to maximize the minimum machine load, we then give two asymptotically optimal algorithm classes which have worst-case ratios very close to the upper bound of the problem for any given m. These results greatly improve the results proposed by He Yong and Tan Zhiyi in 2.02.

关键词： scheduling design and analysis of algorithm worst-case ratio.

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Bernstein theorem for harmonic morphisms from (N + 1)-sphere to N- manifold

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Chinese science Bulletin 1997年第1期42卷 11-14页

作者： DONG YuxinDepartment of Mathematics, hangzhou university, hangzhou 31002., china Department of Mathematics Hangzhou University Hangzhou China

morphism. Harmonic morphisms may be characterized as harmonic mappings which are horizontally weakly conformal. In recent years a substantial progress has been made in the field of harmonic morphism, for example, refs. [ 3—7]. In ref. [4], Baird and Wood proved the following: (ⅰ) Any non-constant harmonic morphism from (S^3, g_(can)) to a Riemann

关键词： harmonic morphism Hopf fibration geodesic.

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Turbulence modulation model for gas–particle flow based on probability density function approach

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Chinese Physics B 2017年第8期26卷 235-241页

作者：王路徐江荣 school of Energy and Power Engineering Nanjing University of Science and Technology Department of Physics Hangzhou Dianzi University

he paper focuses on the turbulence modulation problem in gas–particle flow with the use of probability density function（PDF） approach. By means of the PDF method, a general statistical moment turbulence modulation model without considering the trajectory difference between two phases is derived from the Navier–Stokes equations. A new turbulence production term induced by the dispersed-phase is analyzed and considered. Furthermore, the trajectory difference between two media is taken into account. Subsequently, a new k–ε turbulence modulation model in dilute particle-laden flow is successfully set up. Then, the changes to several terms, including the turbulence production, dissipation, and diffusion terms, are well described consequently. The promoted model provides a more probable explanation for the modification of particles on the turbulence. Finally, we applied the model to simulate a gas–particle turbulence flow case in a wall jet, and found that the simulation results agree well with the experimental data.

关键词： turbulence modulation model PDF approach gas-particle flow turbulence flow

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Organobentonites　as　adsorbents　for　some　organic　pollutants　and　its　application　in　wastewater　treatment

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Journal of Environmental sciences 1996年第3期8卷 378-383页

作者： Zhu Lizhong Zhang Jianying Li Yimin Shen Xueyou Qi Wenbin(Department of Environmental science. hangzhou {2., hangzhou 31002., china) Department of Environmental science Hangzhou University Hangzhou 310028 China

Two organobentonites were synthesized by placing quaternary ammonium cationscetyltrimethylammonium bromide (CTMAB) and cetylpyridinium chloride (CPC) on bentonite bycation exchange. Their ability to adsorb phenol, aniline. nitrobenzene and p-nitrophenol were *** optimal conditions for organobentonites to remove the organic pollutants from waterwere studied. The removal rates for organobentonites to treat the organic compounds in water werefound to be over 8 times for the original mineral (untreated bentonite).The removal rates of organic pollutants and COD of wastewater were further improved by organobentonites in the presence of aluminum sulfate. The structure of organobentonites and the mechanism for their adsorption were investigated by X-ray diffraction (XRD) analysis, infrared spectra and BET surface area.

关键词： organobentonites adsorbents organic pollutants wastewater treatment.

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Effects of Ca substitution and surface treatment on electrochemical performances of Ml_(1.0-x) Ca_xNi_(4.0) Co_(0.6) Al_(0.4) hydrogen storage alloy electrodes

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中国有色金属学会会刊：英文版 1999年第3期9卷 487-492页

作者： Chen Weixiang(陈卫祥) 1, Qi Jianqin(祁建琴) 1, Chen Changpin(陈长聘) 2.1. Department of Physics 2. Department of Materials science and Engineering, Zhejiang university, {2. 31002., P. R. china 浙江大学物理系浙江杭州浙江大学材料科学与工程系浙江杭州

The influences of Ca substitution for Ml and surface treatment on electrochemical performances of Ml 1.0- x Ca x Ni 4.0 Co 0.6 Al 0.4 hydrogen storage alloy electrode were investigated. Ca substitution with x 2.for Ml could improve its discharge capacity, but Ca substitution with x >0.2.could decrease the capacity. It was also found that Ca substitution deteriorates the electrocatalytic activity, high rate dischargeability and cycling durability of the alloy electrode. In order to improve these properties of Ml 1.0- x Ca x Ni 4.0 Co 0.6 Al 0.4 alloy electrode, the alloy was treated in 6 mol/L KOH+0.02.mol/L KBH 4 solution. The results showed that the surface treatment improves the electrochemical performances such as the electrocatalytic activity, high rate dischargeability and cycling durability of the alloy electrode. :The influences of Ca substitution for Ml and surface treatment on electrochemical performances of Ml 1.0- x Ca x Ni 4.0 Co 0.6 Al 0.4 hydrogen storage alloy electrode were investigated. Ca substitution with x 2.for Ml could improve its discharge capacity, but Ca substitution with x >0.2.could decrease the capacity. It was also found that Ca substitution deteriorates the electrocatalytic activity, high rate dischargeability and cycling durability of the alloy electrode. In order to improve these properties of Ml 1.0- x Ca x Ni 4.0 Co 0.6 Al 0.4 alloy electrode, the alloy was treated in 6 mol/L KOH+0.02.mol/L KBH 4 solution. The results showed that the surface treatment improves the electrochemical performances such as the electrocatalytic activity, high rate dischargeability and cycling durability of the alloy electrode.

关键词： hydrogen storage alloy electrode Ca substitution surface modification

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Convergence on the iteration of Halley family in weak conditions

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Chinese science Bulletin 1997年第7期42卷 552-555页

作者： WANG XinghuaDepartment of Mathematics, hangzhou university, hangzhou 31002., china Department of Mathematics Hangzhou University Hangzhou China

WE have obtained the convergence theorems of the iteration of Halley family by the point estimate of Smale. In the point estimate, map f which is desired to be solved is presumed to be analytic in some proper neighbor... 详细信息

关键词： operator equations Banuch space the iteration of Halley family convergence weak conditions.

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The best quadrature based on given Hermite information for the Sobolev class KW^r[a,b]

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science china Mathematics 2006年第8期49卷 1146-1152页

作者： WANG Xinghua & YANG Shijun Department of Mathematics, Zhejiang university, hangzhou 31002., {2. Department of Mathematics, hangzhou Normal College, hangzhou 310036, {2. Department of Mathematics Zhejiang University Hangzhou China Department of Mathematics Hangzhou Normal College Hangzhou China

As usual, denote by KWr[a,b] the Sobolev class consisting of every function whose (r-1)th derivative is absolutely continuous on the interval [a,b] and rth derivative is bounded by K a.e. In [a,b]. For a function f ∈ KWr[a,b], its values and derivatives up to r-1 order at a set of nodes x are known. These values are said to be the given Hermite *** work reports the results on the best quadrature based on the given Hermite information for the class KWr[a,b]. Existence and concrete construction issue of the best quadrature are settled down by a perfect spline interpolation. It turns out that the best quadrature depends on a system of algebraic equations satisfied by a set of free nodes of the interpolation perfect spline. From our another new result, it is shown that the system can be converted in a closed form to two single-variable polynomial equations, each being of degree approximately r/2. As a by-product,the best interpolation formula for the class KWr[a,b] is also obtained.

关键词： Sobolev class, Hermite information, perfect spline, optimal recovery, best quadrature.

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