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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. state key {2. of Freshwater Ecology and Biotechnology, {4. of Hydrobiology, Chinese academy of sciences, Wuhan 4.0072. china 2. Graduate School of Chinese academy of sciences, beijing 100039, china) state key {2. of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of Chinese academy of sciences Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4.amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334.aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4.bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4.month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4.month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4.month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

PARALLEL CHAOTIC MULTISPLITTING ITERATIVE METHODS FOR THE LARGE SPARSE LINEAR COMPLEMENTARITY PROBLEM

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Journal of Computational Mathematics 2001年第3期19卷 281-292页

作者： Zhong-zhi Bai (state key {2. of Scientific/Engineering Competing institute of Computational Mathematics and Scientific/Engineering Computing, {4. of Mathematics and Systems sciences, Chinese {4. of sciences, beijing 100080, china) 中科院计算数学与工程计算数学所科学与工程计算国家重点实验室北京 100080

Focuses on a study which presented a parallel chaotic multisplitting method for solving the large sparse linear complementarity problem. Preliminaries of the study; Equations of the parallel chaotic multisplitting met... 详细信息

关键词： linear complementarity problem matrix multisplitting chaotic iteration relaxed method convergence property

Relative importance of water, energy, and heterogeneity in determining regional pteridophyte and seed plant richness in china

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Journal of Systematics and Evolution 2011年第2期49卷 95-107页

作者： 1,2.3 Sheng-Bin CHEN § 1 Gao-Ming JIANG 3 Zhi-Yun OUYANG 3 Wei-Hua XU 3 Yi XIAO 1 (state key {2. of Vegetation and Environmental Change, {4. of Botany, Chinese academy of sciences, beijing 100093, china) 2.(Graduate University of the Chinese academy of sciences, beijing 10004., china) 3 (state key {2. of Urban and Regional Ecology, Research Center for Eco-Environmental sciences, Chinese academy of sciences, beijing 100085, china) (state key {2. of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate University of the Chinese academy of sciences Beijing 100049 China) (state key {2. of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China)

Environmental variables, such as ambient energy, water availability, and environmental heterogeneity have been frequently proposed to account for species diversity gradients. How taxon-specific functional traits define large-scale richness gradients is a fundamental issue in understanding spatial patterns of species diversity, but has not been well documented. Using a large dataset on the regional flora from china, we examine the contrast spatial patterns and environmental determinants between pteridophytes and seed plants which differ in dispersal capacity and environmental requirements. Pteridophyte richness shows more pronounced spatial variation and stronger environmental associations than seed plant richness. Water availability generally accounts for more spatial variance in species richness of pteridophytes and seed plants than energy and heterogeneity do, especially for pteridophytes which have high dependence on moist and shady environments. Thus, pteridophyte richness is disproportionally affected by water-related variables; this in turn results in a higher proportion of pteridophytes in regional vascular plant floras (pteridophyte proportion) in wet regions. Most of the variance in seed plant richness, pteridophyte richness, and pteridophyte proportion explained by energy is included in variation that water and heterogeneity account for, indicating the redundancy of energy in the study extent. However, heterogeneity is more important for determining seed plant distributions. Pteridophyte and seed plant richness is strongly correlated, even after the environmental effects have been removed, implying functional linkages between them. Our study highlights the importance of incorporating biological traits of different taxonomic groups into the studies of macroecology and global change biology.

关键词： habitat heterogeneity pteridophytes seed plants species richness water-energy hypothesis.

Spatial prediction and analysis of Himalayan marmot plague natural epidemic foci in china based on HJ-1 satellite data

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Science china Earth sciences 2010年第S1期53卷 8-15页

作者： GAO MengXu1,4.LI XiaoWen1,2.CAO ChunXiang1,ZHANG Hao1,LI Qun3,ZHOU Hang3 HE QiSheng1,4.XU Min1,4.ZHAO Jian1,4.ZHENG Sheng1,4.& CHEN Wei1,4.1state key {2. of {4. sensing Science,Jointly Sponsored by the institute of remote sensing applications of Chinese academy of sciences and beijing Normal University,beijing 100101,china 2.chool of Geography,beijing Normal University,beijing 100875,china 3Office for Disease Control and Emergency Response,Chinese Center for Disease Control and Prevention,beijing 102.06,china 4.raduate University of Chinese academy of sciences,beijing 10004.,china state key {2. of remote {4. Science Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University Beijing China Graduate University of Chinese academy of sciences Beijing China School of Geography Beijing Normal University Beijing China Office for Disease Control and Emergency Response Chinese Center for Disease Control and Prevention Beijing China

Plague,caused by the gram-negative bacterium Yersinia pestis,is a serious and rapidly progressing illness in humans that can be fatal if not treated *** Qinghai-Tibet Plateau is the largest area of natural Himalayan marmot(Marmota himalayana) plague foci in china and covers more than 630000 *** County in Gansu Province is a part of this natural focus of plague and was chosen as a study *** study used an ecological niche modeling(ENM) approach to predict the potential distribution of the Himalayan *** and Disaster Monitor Satellite(HJ-1) data was used to investigate environment factors that affect plague host animal *** animal point data from active surveillance was combined with environmental variables from the HJ-1 satellite and other databases,and the models of the potential distribution of Himalayan marmot were produced with the Genetic Algorithm for Rule-Set Production(GARP).The probability of marmot presence was divided into 0-5%,5%-2.%,2.%-4.%,4.%-80%,and 80%-100% *** with 80%-100% probability exhibited the greatest potential for the presence of Himalayan *** to the predicted potential distribution of Himalayan marmot in the study area,active surveillance of plague hosts and plague control and prevention could be more efficient.

关键词： Himalayan marmot plague spatial prediction GARP HJ-1 satellite

Tectono-chronologic constraints on a Mesozoic slip and thrust belt in the eastern Jiaodong Peninsula

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Science china Earth sciences 2007年第1期50卷 25-32页

作者： ZHANG HongYuan1,2. HOU QuanLin3 & CAO DaiYong 4.1 institute of Geology, Chinese academy of Geological {2., {4. 100037, china 2.state key laboratory of Geological Processes and Mineral Resources, china University of Geo{2., {4. 100083, china 3 Graduate School of china Acacemy of {2., {4. 100039, china 4.key laboratory of Coal Resources (CUMT), Ministry of Education, {4. 100083, china institute of Geology Chinese Academy of Geological Sciences Beijing China state key {2. of Geological Processes and Mineral Resources China University of Geosciences Beijing China Graduate School of china academy of {2. Beijing China key laboratory of Coal Resources (CUMT) Ministry of Education Beijing China

A major slip and thrust belt within the eastern Jiaodong Peninsula is located at the eastern terminal of the Qinling-Dabie-Sulu orogenic belt between the Sino-Korea Block and Yangtze Block. Although a lot of isotope chronologic data have been obtained regionally, little structural chronological research has been conducted in this region and this paper corrects that. Syn-deformational minerals were system-atically selected from samples of the NE-ENE trending transpressional shear zones and transpres-sional nappes and carefully analysed using 4.Ar/39Ar methods. Two tectonic events were defined with the first event resulting from early movement of transpressional nappes around 190 Ma ago. This ac-cords with the period of syn-orogenic sinistral slip of the Tan-Lu faults and clockwise shear in the Eastern Qinling-Tongbaishan part of the Qinling-Dabie-Sulu orogenic belt. The second event involved strikeslip thrust movement of deep shear zones between 130Ma and 12.Ma. This resulted from the onset of Mesozoic tectonic conversion in the eastern Jiaodong Peninsula. The sinistral strikeslip-thrusting in Jiaodong Peninsula and the extensional tectonism (toward ESE) in Liaodong Peninsula probably resulted in the clockwise rotation of Korea Peninsula in late Mesozoic.

关键词： the eastern Jiaodong Peninsula tectono-chronology 4.Ar/39Ar strikeslip and thrust belt.

Features and distribution patterns of Chinese endemic seed plant species

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Journal of Systematics and Evolution 2011年第2期49卷 81-94页

作者： 1,2.3 Ji-Hong HUANG 1,4.Jian-Hua CHEN 5 Jun-Sheng YING 1 Ke-Ping MA 1 (state key {2. of Vegetation and Environmental Change, {4. of Botany, Chinese academy of sciences, beijing 100093, china) 2.(Graduate University of Chinese academy of sciences, beijing 10004., china) 3 (Center for Documentation and Information, {4. of Botany, Chinese academy of sciences, beijing 100093, china) 4.(Zhejiang Normal University, Jinhua 32.004. china) 5 (Research Center of Systematic and Evolutionary Botany, {4. of Botany, Chinese academy of sciences, beijing 100093, china) (state key {2. of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate University of Chinese academy of sciences Beijing 100049 China) (Center for Documentation and Information Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Zhejiang Normal University Jinhua 321004 China) (Research Center of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China)

We compiled and identified a list of Chinese endemic seed plant species based on a large number of published references and expert reviews. The characters of these seed plant species and their distribution patterns were described at length. china is rich in endemic seed plants, with a total of 14.939 species (accounting for 52.1% of its total seed plant species) belonging to 1584.genera and 191 families. Temperate families and genera have a significantly higher proportion of endemism than cosmopolitan and tropical ones. The most primitive and derived groups have significantly higher endemism than the other groups. The endemism of tree, shrub, and liana or vine is higher than that of total species; in contrast, the endemism of herb is lower than that of total species. Geographically, these Chinese endemic plants are mainly distributed in Yunnan and Sichuan provinces, southwest china. Species richness and proportion of these endemic plants decrease with increased latitude and have a unimodal response to altitude. The peak value of proportion of endemism is at higher altitudes than that of total species and endemic species richness. The proportions of endemic shrub, liana or vine, and herb increase with altitude and have a clear unimodal curve. In contrast, the proportion of tree increases with altitude, with a sudden increase at ～4.00 m and has a completely different model. To date, our study provides the most comprehensive list of Chinese endemic seed plant species and their basic composition and distribution features.

关键词： biodiversity conservation distribution pattern endemism flora of china seed plants.

Effects of fundamental structure parameters on dynamic responses of submerged floating tunnel under hydrodynamic loads

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Acta Mechanica Sinica 2009年第3期25卷 335-344页

作者： Xu Long Fei Ge Lei Wang Youshi Hong state key {2. of Nonlinear Mechanics, institute of Mechanics, Chinese {4. of sciences, beijing 100190, china state key {2. of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences Beijing China

This paper investigates the effects of structure parameters on dynamic responses of submerged floating tunnel （SFT） under hydrodynamic loads. The structure parameters includes buoyancy-weight ratio （BWR）, stiffness coefficients of the cable systems, tunnel net buoyancy and tunnel length. First, the importance of structural damp in relation to the dynamic responses of SPT is demonstrated and the mechanism of structural damp effect is discussed. Thereafter, the fundamental structure parameters are investigated through the analysis of SFT dynamic responses under hydrodynamic loads. The results indicate that the BWR of SFT is a key structure parameter. When BWR is 1.2. there is a remarkable trend change in the vertical dynamic response of SFT under hydrodynamic loads. The results also indicate that the ratio of the tunnel net buoyancy to the cable stiffness coefficient is not a characteristic factor affecting the dynamic responses of SFT under hydrodynamic loads.

关键词： Submerged floating tunnel Structural dampBuoyancy-weight ratio Cable stiffness coefficient ,Tunnel net buoyancy - Hydrodynamic load

Investigation of fine and complex vortex circulation structures

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Science china Earth sciences 2010年第10期53卷 1552-1558页

作者： LUO ZheXian1,MA GeLan1 & PING Fan2.1 remote sensing College,Nanjing University of Information Science and Technology,Nanjing 2.004.,{2. 2.laboratory of Cloud-Precipitation Physics and Severe Storms(LACS),institute of Atmospheric Physics,Chinese academy of sciences,beijing 10002.,{2. remote sensing College Nanjing University of Information Science and Technology Nanjing China laboratory of Cloud-Precipitation Physics and Severe Storms (LACS) Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China

The formation and evolution of fine and complicated vortex circulation structures were investigated using a two-dimensional quasi-geostrophic barotropic model *** find that the highly localized asymmetric and complex configuration of energy transfer flux between large-and small-scale components is caused by the nonlinear interaction between a large-scale vortex with an initial axi-symmetric flow and four beta meso-scale *** complex structure is characterized by a fine pattern,which contains seven closed systems with spatial scales of less than 100 km,embedded in a positive flux wave train and a negative wave train,*** average wind speed decreased with time in the positive flux region,but was nearly unchanged in the negative flux *** pattern reveals the evolutionary asymmetry and localization of wind speed of the major *** track of the major vortex center has a trend toward the center of the negative flux center,indicating that there is a certain relation between the complex structure of the energy transfer flux and the motion of the major vortex *** results imply that the formation and evolution of the fine and complex structure should be attributed to the nonlinear interaction between the vortices at different spatial scales.

关键词： vortex complex structure non-linear interaction energy cascade

A fusion algorithm for remote sensing images based on nonsubsampled pyramids and bidimensional empirical decomposition

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Science china(Technological sciences) 2010年第S1期53卷 196-204页

作者： ZHANG XiaoDong 1,WANG WenBo 2.WANG DiFeng 3 & ZHANG Yu 4.1 state key {2. of Information Engineering in Surveying,Mapping and {4. sensing,Wuhan University,Wuhan 4.0079,china 2.College of sciences,Wuhan University of Science and Technology,Wuhan 4.0065,china 3state key {2. of Satellite Ocean Environment Dynamics,Second institute of Oceanography,state Oceanic Administration,Hangzhou 310012.china 4.Spatial Information Technology Application Research institute,Changjiang River Scientific Research institute,Wuhan 4.0010,china state key {2. of Information Engineering in Surveying Mapping and Remote Sensing Wuhan University Wuhan China College of sciences Wuhan University of Science and Technology Wuhan China state key {2. of Satellite Ocean Environment Dynamics Second Institute of Oceanography State Oceanic Administration Hangzhou China Spatial Information Technology Application Research institute Changjiang River Scientific Research Institute Wuhan China

In order to improve the quality of remote sensing image fusion,a new method combining nonsubsampled Laplacian pyramid (NLP)and bidimensional empirical mode decomposition(BEMD)is ***,the high resolution panchromatic image (PAN)is decomposed using NLP until the approximate component and the low resolution multispectral image(MS)contain features with a similar ***,the approximation component and the MS are decomposed by BEMD,resulting in a number of bidimensional intrinsic mode functions(BIMF)and a residue *** instantaneous frequency is computed in 4.directions of the *** the positive or negative coefficients in the corresponding position,a weighted algorithm is designed for fusing the high frequency details using the instantaneous frequency and the coefficient absolute value of the BIMFs as fusion *** fused image is then obtained through inverse BEMD and *** results have illustrated the advantage of this method over the IHS,DWT andà-Trous wavelet in both spectral and spatial detail qualities.

关键词： bidimensional empirical mode decomposition nonsubsampled pyramid instantaneous frequency remote sensing image fusion