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Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L.) with RAPD markers

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Progress in Natural science:Materials International 2005年第2期15卷 48-53页

作者： LI Xiaozun1*, PAN Junsong1*, WANG Gang1, TIAN Libo3, SI Longting3, WU Aizhong2.* and CAI Run1**(1. Agriculture and Biology School, shanghai Jiaotong University, shanghai 2.1101,{2. 2. shanghai academy of agricultural science, shanghai 2.1106,{2. 3. Horticulture School, Shenyang agricultural University, Shenyang 110161,{2.) Agriculture and Biology School Shanghai Jiaotong University Shanghai 201101 China. Horticulture School Shenyang Agricultural University Shenyang 110161 China. shanghai academy of {2. science Shanghai 201106 China

A cucumber (Cucumis sativus L.) molecular linkage map, including 79 random-amplified polymorphic DNAs (RAPD) and two genes , lb for lateral branch and f for female sex expression, is constructed from a cross between a line, S52. with weak lateral growing ability and staminate from Dabieshan Mountains area in china and another line, S06, with strong lateral growing ability and gynoecious from Europe. The map contains nine linkage groups and spans 1110.0 cM with an average distance of 13.7 cM between loci. The lb locus is located in a longer linkage group LG-2.and flanked by two markers, OP-Q5-1 and OP-M-2.2. at 9.3 cM and 15.9 cM, respectively. In the meantime, the RAPD loci, OP-Q5-2.and BC151, in a short linkage group were found to flank f at 13.7 cM and 13.4 cM, respectively. The construction of RAPD map has paved a way for further study of the genes for lateral branch, female sex expression and other agronomic traits in cucumber.

关键词： cucumber (Cucumis sativus L.) lateral branch gynoecy RAPD genetic mapping

Boundary element-free method for elastodynamics

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science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 641-657页

作者： CHENG Yumin1 & PENG Miaojuan2.1. shanghai Institute of Applied Mathematics and Mechanics, shanghai University, shanghai 2.0072. china 2. Department of Civil Engineering, shanghai University, shanghai 2.0072. china 1. shanghai Institute of Applied Mathematics and Mechanics Shanghai University 200072 Shanghai China 2. Department of Civil Engineering Shanghai University 200072 Shanghai China

The moving least-square approximation is discussed first. Sometimes the method can form an ill-conditioned equation system, and thus the solution cannot be obtained correctly. A Hilbert space is presented on which an orthogonal function system mixed a weight function is defined. Next the improved moving least-square approximation is discussed in detail. The improved method has higher computational efficiency and precision than the old method, and cannot form an ill-conditioned equation system. A boundary element-free method (BEFM) for elastodynamics problems is presented by combining the boundary integral equation method for elastodynamics and the improved moving least-square approximation. The boundary element-free method is a meshless method of boundary integral equation and is a direct numerical method compared with others, in which the basic unknowns are the real solutions of the nodal variables and the boundary conditions can be applied easily. The boundary element-free method has a higher computational efficiency and precision. In addition, the numerical procedure of the boundary element-free method for elastodynamics problems is presented in this paper. Finally, some numerical examples are given.

关键词： moving least-square approximation, improved moving least-square approximation, elastodynamics, boundary integral equation, meshless method, boundary element-free method, Fourier eigen transform.

CO observations of massive star forming regions at different evolutionary phases

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science china(Physics,Mechanics & Astronomy) 2006年第5期49卷 626-640页

作者： CHEN Xi1,2. XU Ye1,3, SHEN Zhiqiang1,3 & LI Jingjing1,2.1. shanghai Astronomical Observatory, Chinese academy of {2.s, shanghai 2.0030, china 2. Graduate School of the Chinese academy of {2.s, Beijing 100034, china 3. Joint Institute for Galaxy and Cosmology, shanghai Astronomical Observatory and the University of {2. and Technology of china, shanghai 2.0030, china shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Graduate School of the Chinese academy of sciences Beijing 100034 China Joint Institute for Galaxy Cosmology Shanghai Astronomical Observatory the University of science Technology of china Shanghai 200030 China

The 12.O (J=1-0), 13CO (J=1-0) and C18O (J=1-0) emissions in 9 massive star forming regions, which are believed to be at different stages of massive star formation, were mapped with the 13.7 m millimeter wave telescope at Qinghai Station of Purple Mountain Observatory. Of the observed 9 sources, 13CO cores were detected in seven of them, and C18O cores in five of them. And only two sources associated with C18O cores and H2. masers showed the extended structures and strong outflows. This is the first detection of outflow associated with IRAS 2.566+582. in the observing field of S152.S153. The physical parameters of cores and outflows for these sources, derived from Local Thermal Equilibrium (LTE) analysis, are presented. These observing results suggest that the C18O cores will only appear when the gas density is high enough and the probability to have an outflow is very high when the clumps show the C18O and H2. maser simulta- neously.

关键词： star formation cloud core outflow H2. maser

Identification and Characterization of hmr19 Gene Encoding a Multidrug Resistance Efflux Protein from Streptomyces hygroscopicus *** Strain 10-2.

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 519-528页

作者： Lei QIN , Heng-An WANG1 , Zhong-Qin WU, Xiao-Feng ZHANG, Mei-Lei JIN,Zi-Xin DENG2. and Guo-Ping ZHAOshanghaiResearch Center of Biotechnology, shanghai Institute for Biological {2.s, Chinese academy of {2.s,Graduate School of the Chinese academy of {2.s, shanghai 2.02.3, china 1School of Agriculture and Biology, shanghai Jiaotong University, shanghai 2.1101, china 2.chool of Life {2. and Biotechnology, shanghai Jiaotong University, shanghai 2.0030, china shanghaiResearch Center of Biotechnology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences School of Agriculture and Biology Shanghai Jiaotong University

The hmr19 gene was cloned from Streptomyces hygroscopicus subsp. yingchengensis strain 10- 2., a bacterium strain producing agricultural antibiotics. Sequence similarity comparison indicates that hmr19 gene may encode a predicted protein with 14 putative transmembrane α-helical spanners, belonging to the drug:H+ antiporter-2.family of the major facilitator superfamily. The expression of hmr19 in the mycelium of strain 10-2. was detected by Western blotting analysis. Gene replacement technology was employed to construct an hmr19 disruption mutant. The growth inhibition test against different antibiotics indicated that the mutant strain was 5–2. fold more susceptible to tetracycline, vancomycin and mitomycin C than the parental wild type strain. The mutant took up tetracycline much faster and accumulated more antibiotics than the wild type strain 10-2.. While with the addition of an energy uncoupler, carbonyl cyanide m- chlorophenylhydrazone, the characteristics of the accumulation of [3H]tetracycline in these two strains were almost the same. It was thus concluded that hmr19 encoded a multidrug resistance efflux protein.

关键词： multidrug resistance (MDR) gene replacement (knockout) Streptomyces Streptomyces hygroscopicus subsp. yingchengensis strain 10-2.

Ni2. treatment causes cement gland formation in ectoderm explants of Xenopus laevis embryo

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Cell Research 1999年第1期9卷 71-76页

作者： HUANG YONG XIAO YAN DING (shanghai Institute of Cell Biology, Chinese academy of {2., shanghai 2.0031, china) 1shanghai Institute of Cell Biology Chinese Academy of Science Shanghai 200031 China 1shanghai Institute of Cell Biology Chinese Academy of Science Shanghai 200031 China

We found T-type calcium channel blocker Ni2. can efficiently induce the formation of cement gland in Xenopus laevis animal cap explants. Another T-type specific calcium channel blocker Amiloride can also induce the formation of cement gland, while L-type specific calcium channel blocker Nifedipine has no inductive effect. These results may offer us an new approach to study the differentiation of cement gland through the change of intracelluax calcium concentration.

关键词： Ni2. cement gland gastrulation T-type calcium channel

Construction of a genetic map and localization of QTLs for yield traits in tomato by SSR markers

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Progress in Natural science:Materials International 2005年第9期15卷 793-797页

作者： LIU Yang 1,2., CHEN Huoying1**, WEI Yutang2.and ZHUANG Tianming1(1. Department of Plant science, shanghai Jiaotong University, shanghai 2.1101, china 2. Department of Horticulture, Shenyang agricultural University, Shenyang 110161, china) Department of Plant science Shanghai Jiaotong University Shanghai 201101 China. Department of Horticulture Shenyang Agricultural University Shenyang 110161 China

Using an F2.opulation derived from the hybrid of Lycopersicon esculentum Mill. ‘XF 98-7’× Lycopersicon pimpinellifolium LA2.84, a SSR genetic linkage map of tomato is constructed. The map contains 112.markers and spans 808.4 cM with an average distance of 7.2. cM between loci. Two quantitative trait loci （QTLs） for first flower node on chromosomes 5 and 11, two QTLs for number of flowers per truss on chromosomes 2.and 5, and five QTLs for fruit weight on chromosomes 1, 2. 3, 9 and 12.are identified.

关键词： tomato SSR markers genetic mapping first flower node number of flowers per truss fruit weight.

An interpolating boundary element-free method (IBEFM) for elasticity problems

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science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 758-766页

作者： REN HongPing 1 , CHENG YuMin 2.& ZHANG Wu 1 1 School of Computer Engineering and science, shanghai University, shanghai 2.0072. china 2.shanghai Institute of Applied Mathematics and Mechanics, shanghai University, shanghai 2.0072. china 1. School of Computer Engineering and science Shanghai University Shanghai 200072 China2. Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai 200072 China

The paper begins by discussing the interpolating moving least-squares (IMLS) method. Then the formulae of the IMLS method obtained by Lancaster are revised. On the basis of the boundary element-free method (BEFM), combining the boundary integral equation method with the IMLS method improved in this paper, the interpolating boundary element-free method (IBEFM) for two-dimensional elasticity problems is presented, and the corresponding formulae of the IBEFM for two-dimensional elasticity problems are obtained. In the IMLS method in this paper, the shape function satisfies the property of Kronecker δ function, and then in the IBEFM the boundary conditions can be applied directly and easily. The IBEFM is a direct meshless boundary integral equation method in which the basic unknown quantity is the real solution to the nodal variables. Thus it gives a greater computational precision. Numerical examples are presented to demonstrate the method.

关键词： moving least-squares (MLS) approximation interpolating moving least-squares (IMLS) method boundary integral equation meshless method boundary element-free method (BEFM) interpolating boundary element-free method (IBEFM) elasticity problem

A MONOTONE COMPACT IMPLICIT SCHEME FOR NONLINEAR REACTION-DIFFUSION EQUATIONS

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Journal of Computational Mathematics 2008年第2期26卷 123-148页

作者： Yuanming Wang Department of Mathematics,East china Normal University,shanghai 2.02.1,{2. Division of Computational science,E-Institute of shanghai Universities,shanghai Normal Benyu Guo Department of Mathematics,shanghai Normal University,shanghai 2.02.4,{2. Division of Computational science,E-Institute of shanghai Universities,shanghai,{2. Department of Mathematics East China Normal University Shanghai 200241 China Division of Computational science E-Institute of Shanghai Universities Shanghai Normal University Shanghai 200234 China Department of Mathematics Shanghai Normal University Shanghai 200234 China Division of Computational science E-Institute of Shanghai Universities Shanghai China

A monotone compact implicit finite difference scheme with fourth-order accuracy in space and second-order in time is proposed for solving nonlinear reaction-diffusion equations. An accelerated monotone iterative method for the resulting discrete problem is presented. The sequence of iteration converges monotonically to the unique solution of the discrete problem, and the convergence rate is either quadratic or nearly quadratic, depending on the property of the nonlinear reaction. The numerical results illustrate the high accuracy of the proposed scheme and the rapid convergence rate *** iteration.

关键词： Nonlinear reaction-diffusion equation Monotone compact implicit scheme High accuracy Monotone iteration Rapid convergence rate.

Chinese Jingdezhen blue and white imperial porcelain

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science china(Technological sciences) 2004年第3期47卷 366-375页

作者： WU Juan1, LI Jiazhi1, DENG Zequn1 & WANG Changsui2.1. Laboratory of Ancient Ceramics, shanghai Institute of Ceramics, Chinese academy of {2.s, shanghai 2.0050, china 2. Department of History of {2. and Archaeometry, University of {2. and Technology of china, Hefei 2.002., china Chinese Acad Sci Shanghai Inst Ceram Lab Ancient Ceram Shanghai 200050 Peoples R China Univ Sci & Technol china Dept Hist Sci Archaeometry Hefei 230026 Peoples R China

Jingdezhen blue and white imperial porcelain specimens from the Yuan, Ming and Qing dynasties have been systematically analyzed using a nondestructive test method—— energy dispersive X-ray fluorescence analysis (EDXRF). The derived data of major and trace element compositions have been treated by correspondence analysis. The variation laws of the composition patterns for Jingdezhen blue and white imperial porcelain in different historical periods owing to the change in raw materials, recipe and technology have been discussed, and a time model related to variation of element composition has been preliminarily established, It would be helpful for scientific dating of Jingdezhen blue and white imperial porcelain, and even for the studies on the whole field of identification of ancient ceramics.

关键词： energy dispersive X-ray fluorescence analysis, blue and white imperial porcelain, correspondence analysis.