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Rapid Detection ofK-ras Gene Point Mutation at Codon 12.by PCR-SSPin Pancreatic Adenocarcinom a

The Journal of Biomedical research 1999年第2期23卷 78-80页

作者： Liu Xunliang (刘训良) Dai Cuncai (戴存才) Du Jinghui (杜竞辉) Miao Yi (苗毅) Zhang Zhaosong (张兆松) 1 Cheng Shuzhen (陈淑贞) 1 Wang Xiang (王翔) 1 Department of general surgery, the First Affiliated {2. of {3. Medical University, {3. 2.002., P.R. China 1Molecular Biology research Center, {3. Medical University, {3. 2.002., P.R. China

To evaluate the feasibility and clinical significance of the PCR SSP technique in detecting K ras gene mutation at codon 12.in pancreatic adenocarcinoma tissues. 80 specimens of surgical resection or biopsy samples were tested at our hospital from January 1994 to September 1995. Three different special sequence primers (SSP) synthesized according to mutation styles of CGT, GTT, GAT were respectively prepared. Three amplification reactions were performed for each sample. The amplification products were analyzed by conventional polyacrylamide gel electrophoresis, stained with ethidium bromide and observed under UV transillumination. Results: All of the 3. pancreatic adenocarcinoma samples had positive PCR results with the mutation rate 100%. 7 cases were CGT mutation, 18 GGT and 17 GAT mutation, in which 2.types of mutation existed in 8 cases. No mutation appeared in 13.normal pancreatic tissues, 6 insulinomas, 6 chronic pancreatitis, 5 benign pancreatic cysts, 7 bile duct carcinoma, 5 ampulla carcinoma and 4 carcinomas of duodenal papilla. Conclusion: Pancreatic adenocarcinoma is one of the commonly encounted tumors and is still very difficult to diagnose at the early stage and to distinguish from other lesions preoperatively. Our study indicates that PCR SSP is an ideal assay in comparison with other methods to detect K ras gene mutation. It is simple, rapid, specific, sensitive and easily {3.ized for clinical application on preoperative diagnosis.

关键词： pancreatic adenocarcinoma RCP SSP K ras gene point mutation

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高脂血症及肝脏脂肪变性调节相关因素的研究

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胃肠病学和肝病学杂志 2010年第4期19卷 355-357页

作者：施慧刘炯汪芳裕南京军区南京总医院消化内科江苏南京210002

目的探讨雌激素缺乏、高脂饮食、运动等因素对高脂血症形成及肝脏脂肪变性的调节作用。方法本实验采用C57BL/6J雌性小鼠,随机分为:正常对照组,OVX(卵巢切除后雌激素缺乏)组、OVX+高脂组、OVX+运动组、OVX+高脂+运动组,观察和分析雌激素... 详细信息

目的探讨雌激素缺乏、高脂饮食、运动等因素对高脂血症形成及肝脏脂肪变性的调节作用。方法本实验采用C57BL/6J雌性小鼠,随机分为:正常对照组,OVX(卵巢切除后雌激素缺乏)组、OVX+高脂组、OVX+运动组、OVX+高脂+运动组,观察和分析雌激素缺乏、高脂饮食、运动等因素对小鼠血脂及相关的肝脏脂肪变性的影响。结果各组小鼠血脂水平及肝脏组织学改变的结果显示:OVX组较正常对照组血脂升高明显(P<0.01),而OVX+高脂组又较OVX组血脂升高明显(P<0.05),OVX+运动组较OVX组血脂降低明显(P<0.05),其脂肪肝变性程度也较轻;OVX+高脂+运动组较OVX+高脂组血脂明显降低(P<0.01),较OVX+运动组血脂明显升高(P<0.05),OVX+高脂组、OVX+高脂+运动组小鼠脂肪肝变性程度较重。血脂水平与肝脏脂肪变性程度有相关的趋势。结论雌激素缺乏、高脂饮食增加小鼠血脂水平及加重肝细胞脂肪沉积,运动可以降低去势小鼠血脂水平及肝脏脂肪沉积。

关键词：脂肪肝血脂运动雌激素

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Work-family conflict and job burn-out among Chinese doctors:the mediating role of coping styles

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general Psychiatry 2018年第4期31卷 24-30页

作者： Ling Chen Junjun Liu Hailong Yang Hui Ma Hui Wang Yun Huang Hao Cheng Dianzhen Tang Miao Liu Houyuan Luo Haitao Qu Diwen Shen Ning Zhang Department of Clinical Psychology Jiangsu Province Geriatric InstituteNanjingChina Department of Psychiatry Nanjing Meishan HospitalNanjingChina Department of Clinical Psychology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical UniversityNanjingChina Department of Clinical Psychology Nanjing Brain Hospital Affliated to Nanjing Medical UniversityNanjingChina Department of Neurology Lianyungang hospital of Traditional Chinese Medicine LianyungangChina Medical Insurance Audit Department Medical Insurance Management and Service BureauHangzhouChina Educational and Counseling Department of Mental Health Nanjing University of Posts and TelecommunicationsNanjingChina Customer Service Department Tuniu CorporationNanjingChina College of Education Weifang University of Science and TechnologyHuaifangChina Department of Educational Psychology University of AlbertaEdmontonAlbertaCanada institute of Health Management Jiangsu Jiankang Vocational CollegeNanjingChina

Background Burn-out among doctors threatens their own health, and that of their patients. To identify risk factors of the doctor burn-out is vital to improving their health and increasing the quality of healthcare services. This study aims to explore the relationship between work-family confict （WFC） and burn-out among Chinese doctors and the mediating role of coping styles in this *** A cross-sectional survey was conducted in China, with a questionnaire packet which consisted of the Chinese Maslach Burnout Inventory （CMBI）, WFC Scale and the Simplifed Coping Style Questionnaire （SCSQ）. A total of 2.3. doctors participated in the survey. Correlation analysis was performed to explore the relationship between CMBI, WFC and SCSQ scores. A linear regression model was set to determine the mediating role of coping styles on the relationship between WFC and *** Doctors who had higher scores on work interfering with family confict, reported more emotional exhaustion （r=0.514, P〈0.001） and had a sense of accomplishment （r= 0.149, P〈0.001）. Simultaneously, family interfering with work （FIW） was positively associated with all dimensions of burn-out （r=0.2.3. 0.504, 0.088, respectively, P〈0.001）. Coping styles had partial, complete and even mediating effects on the relationship between WFC and burn-out among Chinese *** WFC was correlated with burn-out, and coping style was a mediator in this relationship among Chinese doctors. Coping style was a positive resource against burn-out.

关键词： Work-family confict and job burn-out among Chinese doctors the mediating role of coping styles

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Early Percutaneous Transhepatic Gallbladder Drainage Compared with ERCP and Papiliotomy Treatment for Severe Gallstones-Associated Acute Pancreatitis

Early Percutaneous Transhepatic Gallbladder Drainage Compare...

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中华医学会第十一届全国胰腺外科学术研讨会

作者： Yu Wen-Kui Li Wei-Qin Wang Zhi-Ming Ye Xiang-Hong Ning Li Jie-Shou Li.Medical College of nanjing University,research {2. of {3. surgery,Jinling hospital,Naniing People''s Republic of China

＜正＞Objective:There is general agreement that early endoscopic retrograde cholangio-pancreatography with or without endoscopic sphincterotomy has been advocated to reduce complications in patients presenting with a severe attack of gallstone-associated acute pancreatitis（GAP）.However,some patients cannot suffer for endoscopic intervention, especially in those early severe acute pancretitis who developed organ failure within 72.hours after the onset of *** compared early Percutaneous transhepatic gallbladder drainage（PTGD）with endoscopic treatment（within 72.hours after the onset of symptoms）in patients with severe biliary panereatitis,to estimate curative effect of PTGD to prevent bile track complications in these ***:Eligible patients were randomized by a blinded pharmacist to receive early treatment with PTGD or *** the initially emergency endoscopic or PTGD treatment was defeated,patients were received another drainage within 2. *** November 2.01 to August 2.05,101 patients were randomly assigned to either early PTGD（51 patients）or ERCP management（50）.Overall mortality,mortality due to pancreatitis,and complications were compared in the two ***:In the first-treatment step,early ERCP was failure in 4 of the 50 patients,and all of the 4 patients were successful in instead of PTGD ***,early PTGD treatment was failure in 2.of the 51 patients,and 2.patients were successful in instead of endoscopic ***,48 of 52.patients were successful in ERCP treatment,53.of 55 were successful in PTGD *** paitients（6.9 percent）-3.in the endoscopic-treatment group and 4 in the PTGD group-died within four months after the onset of pancreatitis（P=0.798）;3.patients in the endoscopic group and 3.in the PTGD group died from acute biliary *** overall rate of complications was similar in the two groups,both the incidence of local complication and systemic complication were no major *** PT

关键词： Percutaneous gallbladder drainage Gallstone-associated acute pancreatitis Endoscopic treatment Complication and outcome

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基于案例推理的ICU应急诊断系统

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系统工程理论与实践 2002年第3期22卷 137-142页

作者：于跃海郑瑞强何建敏东南大学系统工程研究所江苏南京210096 南京铁道医学院附属医院ICU 江苏南京210009

依据应急治疗的医学知识和实践经验 ,综合运用案例推理、模糊推理和缺省推理等方法 ,对重症监护病房中的多种危险病症采取病因和病症相结合的诊治思路进行推理机制的设置 ,建立基于案例推理、模糊推理和缺省推理相结合的 ,能迅速确定重... 详细信息

依据应急治疗的医学知识和实践经验 ,综合运用案例推理、模糊推理和缺省推理等方法 ,对重症监护病房中的多种危险病症采取病因和病症相结合的诊治思路进行推理机制的设置 ,建立基于案例推理、模糊推理和缺省推理相结合的 ,能迅速确定重症监护治疗方案的推理模型 .建立了某医院应急治疗系统的原形系统 .

关键词：案例推理 ICU 应急诊断系统不确定推理重症监护病房医学

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Inhibition of G9a by a small molecule inhibitor, UNC0642.induces apoptosis of human bladder cancer cells

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Acta Pharmacologica Sinica 2019年第8期40卷 1076-1084页

作者： Yue-peng Cao Jing-ya Sun Mei-qian Li Yu Dong Yuan-heng Zhang Jun Yan Rui-min Huang Xiang Yan Department of Urology Nanjing Drum Tower HospitalNanjing University Medical School Shanghai institute of Materia Medica Chinese Academy of Sciences University of Chinese Academy of Sciences MOE Key Laboratory of Model Animals for Disease Study Model Animal Research Center of Nanjing University Shanghai University

Urinary bladder cancer（UBC）is characterized by frequent recurrence and metastasis despite the standard chemotherapy with gemcitabine and cisplatin *** modifiers are often dysregulated in cancer development,thus they can serve as an excellent drug targets for cancer ***,we investigated whether G9a,one of the histone H3.methyltransferases,was associated with UBC *** first analyzed clinical data from public databases and found that G9a was significantly overexpressed in UBC *** TCGA Provisional dataset showed that the average expression level of G9a in primary UBC samples（n = 408）was 1.6-fold as much as that in normal bladder samples（n = 19;P2. and J82.cell lines in vitro,and observed that the cell viability was significantly decreased and cell apoptosis ***,we choosed UNC0642.a small molecule inhibitor targeting G9a,with low cytotoxicity,and excellent in vivo pharmacokinetic properties,to test its anticancer effects against UBC cells in vitro and in *** with UNC0642.dose-dependently decreased the viability of T2.,J82.and 563. cells with the IC50 values of 9.85±0.41,13.15±1.72.and 9.57±0.3. μM,***,treatment with UNC0642.1-2. μM）dose-dependently decreased the levels of histone H3.K9 me2.the downstream target of G9a,and increased apoptosis in T2. and J82.*** nude mice bearing J82.engrafts,administration of UNC0642.5 mg/kg,every other day,i.p.,for 6 times）exerted significant suppressive effect on tumor growth without loss of mouse body ***,administration of UNC0642.significantly reduced Ki67 expression and increased the level of cleaved Caspase 3.and BIM protein in J82.xenografts evidenced by immunohistochemistry and western blot analysis,*** together,our data demonstrated that G9a may be a promising therapeutic target for UBC,and an epigeneticsbased therapy by UNC0642.is suggested.

关键词： human urinary bladder cancer G9a UNC0642 apoptosis

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医用高压氧舱新技术在旧舱改造中的应用

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医学研究生学报 2003年第6期16卷 429-432页

作者：曹锦泉柯文海南京军区南京总医院高压氧科江苏南京210002 芜湖潜水装备厂安徽芜湖241001

目的 :根据我院使用近 3.年的旧氧舱结构特点和安全性能、使用现状 ,科学合理地应用新技术进行改造 ,使改造后的氧舱在安全性和使用性方面更具有先进性 ,技术含量高 ,继续保持国内氧舱的一流水平。　方法 :正确理解现行国家标准和规定 ... 详细信息

目的 :根据我院使用近 3.年的旧氧舱结构特点和安全性能、使用现状 ,科学合理地应用新技术进行改造 ,使改造后的氧舱在安全性和使用性方面更具有先进性 ,技术含量高 ,继续保持国内氧舱的一流水平。　方法 :正确理解现行国家标准和规定 ,充分调研论证 ,精心设计改造方案 ,并经地方主管部门批准后 ,采用招、投标方法 ,选定改造施工单位。施工中邀请地方有关主管部门和本院专业人员实地监督检查。改造结束后 ,邀请省市主管部门和高压氧技术专家们进行验收。　结果 :验收报告指出 ,该氧舱修理改造方案设计合理 ,工艺先进 ,增加了多项先进技术。经改造后的氧舱具有很高的安全性、舒适性 ,有些技术指标已超过国内现有标准 ,达到了国内大型医用氧舱的先进水平。　结论 :旧氧舱完全可改造成为技术先进。

关键词：高压氧舱改造新技术应用

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Expression and characterization of biologically active recombinant human nerve growth factor

Expression and characterization of biologically active recom...

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医药生物技术国际研讨会

作者： Li Su-Qin,Pan Ming-Jie,Pan Ying,Li Yue-Xi~(**) (institute of Military Medicine,Key Laboratory of Medical Biotechniques of Jiangsu Province, nanjing Jinling {2.,nanjing,210002,China)

DNA fragment encoding proNGF was amplified by PCR from genomic DNA isolated from term placental tissue and was cloned into *** sequencing indicated that the cloned DNA fragment was identical as previously *** cells were then tranfected with the recombinant *** cultured in the selection medium containing G418 for 3. *** expressed product in the culture supernatant was *** culture supernatant of the cells harboring recombinant plasmid showed strong ability to promote the outgrowth of nerve fiber from the chicken dorsal root *** results of ELISA showed that the concentration of the recombinant human NGF in the culture supernatant was about 0.5 mg/*** specific activitiy was about 4×10 units per *** conclusion,the proNGF gene was successfully cloned and the protein was correctly expressed and processed in the CHO *** expressed rhNGF has a high specific bioactivity.

关键词： recombinant human NGF PCR CHO cells eukaryotic expression

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重症急性胰腺炎腹腔镜下置双套管引流的观察与护理

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中国实用护理杂志 2005年第8期21卷 4-6页

作者：叶向红嵇武虞文魁李维勤彭南海南京军区南京总医院普通外科研究所ICU 210002

目的探讨重症急性胰腺炎(SAP)早期腹腔镜下置双套灌洗引流管(LPLD)的护理难点及护理措施,逐步形成护理常规。方法摸索出一套规范的腹腔镜下置双套引流管的护理方法与技巧,对2.例腹腔镜下置双套引流管的SAP患者进行观察与护理。结果术后... 详细信息

目的探讨重症急性胰腺炎(SAP)早期腹腔镜下置双套灌洗引流管(LPLD)的护理难点及护理措施,逐步形成护理常规。方法摸索出一套规范的腹腔镜下置双套引流管的护理方法与技巧,对2.例腹腔镜下置双套引流管的SAP患者进行观察与护理。结果术后双套管引流时间(11.3.8.6)d,术后住院时间(3.0}5±14.6)d,2.例治愈出院,1例术后4d死于多器官功能障碍综合征(MODS)。2.例患者均达到预期引流效果。结论LPLD是SAP早期治疗中一种安全可行的方法,它用微创的方法达到引流的目的。

关键词：重症急性胰腺炎腹腔镜双套管引流技术病情观察护理技术

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医院虚拟学科利益分配模型研究

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中国卫生经济 2011年第3期30卷 63-64页

作者：徐幻刘玉秀杨国斌易学明南京军区南京总医院南京210002

虚拟学科的利益分配机制是影响虚拟学科运作的重要条件。缺少有效的利益分配方案极易导致合作伙伴之间的冲突。文章提出了基于贡献和风险的利益收入分配模型, 指出了在不同的合作模式下,应有不同的利益分配模型与之对应,从而为虚拟学科... 详细信息

虚拟学科的利益分配机制是影响虚拟学科运作的重要条件。缺少有效的利益分配方案极易导致合作伙伴之间的冲突。文章提出了基于贡献和风险的利益收入分配模型, 指出了在不同的合作模式下,应有不同的利益分配模型与之对应,从而为虚拟学科合理利益分配提供了参考依据。、

关键词：虚拟学科医院利益分配

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