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Determination of cell volume during equilibrium freezing process

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Science Bulletin 2003年第15期48卷 1551-1554页

作者： ZHAO Gang1, HE Liqun1, WANG Peitao2. DING Weiping1, XIE Xiaojian1, LIU Zhong3, ZHANG Haifeng1, SHU Zhiquan1, LUO Dawei1 & GAO Dayong1,4 1. Cryobiomedical Engineering Research Institute, Department of Thermal Science and Energy Engineering, University of Science and Technology of china, hefei 2.002., china 2. School of Life Sciences, University of Science and Technology of china, hefei 2.002., china 3. red cross and blood center of hefei, hefei 230022, china 4. Department of Mechanical Engineering, University of Kentucky, Lexington, KY 40506-0108, USA Correspondence should be addressed to Zhao Gang (e-mail: cooky@ ***) Cryobiomedical Engineering Research Institute Department of Thermal Science and Energy Engineering University of Science and Technology of China Hefei 230027 China School of Life Sciences University of Science and Technology of China Hefei 230027 China red cross and {2. center of hefei Hefei 230022 China Department of Mechanical Engineering University of Kentucky Lexington KY 40506-0108 United States

A new type electronic particle counter (EPC, MultisizerTM 3, Beckman Coulter Inc., USA) was used to determine the volumes of human red blood cells (RBCs) in NaCl solutions of different osmolalities. The thermodynamics model describing cell response during freezing process was used to simulate the volume change of RBC in 0.9% NaCl solution during equilibrium freezing process. It was assumed that the effect of temperature on cell volume can be neglected compared to that of osmolality, then by using the phase diagram for the binary system sodium chloride/water, the osmolalities of the NaCl solution under different sub-zero temperatures can be obtained (converted from mass concentration), then the calculated values of RBC volumes can be validated by the experiments.

关键词： EPC osmolality RBC Van抰 Hoff cell volume.

Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

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Neural Regeneration Research 2006年第7期1卷 618-621页

作者： Yong Man1, Jianbin Li2. Bo Yang3, Ji Ma1 1Department of Cerebral Surgery, the Second People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, china 2.1} {2. blood center of Henan Province, Zhengzhou 450000, Henan Province, china 3Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, china Department of Cerebral Surgery The Second People's Hospital of Zhengzhou Zhengzhou 450000 Henan Province China red cross {2. center of Henan Province Zhengzhou 450000 Henan Province China Department of Neurosurgery Hospital of Zhengzhou University Zhengzhou 450000 Henan Province China

BACKGROUND: Transplanted mononuclear cell (MNC) of umbilical blood can survive in central nervous system (CNS) of host through blood brain barrier, differentiate into nerve cells, migrate to damaged site and integrate morphological structure and function with nerve cells of host so as to improve deficiencies of sensatory function, motor function and cognitive function and influence on stroke sequela. OBJECTIVE: To observe the vein transplantation of human umbilical cord blood stem cells (HUCBSC) for improving neurological function, limb function and activity of daily living of patients with stroke and evaluate the reliability. DESIGN: Self-controlled study. SETTING: Department of Neurosurgery, the Second People's Hospital of Zhengzhou City; red-crossed blood center of Henan Province; Department of Neurosurgery, the Fist Affiliated Hospital of Zhengzhou University. PARTICIPANTS: A total of 10 patients with stroke sequela were selected from Department of Cerebral Surgery, the Second People's Hospital of Zhengzhou City from April to December 2.05. There were 9 males and 1 female aged from 35 to 75 years with the mean age of 56 years. All of them were diagnosed with CT and MRI examination and coincidence with diagnostic criteria of stroke established by the Fourth National Academic Meeting for Cerebrovascular Disease. All patients provided informed consent. METHODS: 80-140 mL umbilical blood of term birth of newborn was selected hermetically and maintained in sterile plastic bag. And then, the blood was centrifugated at the speed of 1 500 r/min for 30 minutes at 2. ℃ in order to separate MNC, i.e., HUCBSC. In addition, after final diagnosis during hospitalization, stroke patients were perfused with HUCBSC through superficial vein of back of the hand. Each patient was averagely perfused with 6 portions of HUCBSC (cellular numbers ≥ 1×108/portion) and the interval between each portion was 1-7 days with the mean interval of 4 days. MAIN OUTCOME MEASURES: ① Neurological fun

关键词： cell stem Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

Non-freeze-drying injuries of freeze dried living cells

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Non-freeze-drying injuries of freeze dried living cells

胶束薄层荧光法同时测定体液中诺氟沙星和氟罗沙星含量的研究

第六届全国低温生物医学及器械学术大会

作者： Zhiquan Shu~(1,2.,Liu He~1,Dayong Gao~2.Haifeng Zhang~1,Zhong Liu~3,and Liqun He~(*1) 1 Dept.of Thermal Science and Energy Engineering,University of Science and Technology of china,hefei, 2.002.,{2. 2.Department of Mechanical Engineering,University of Washington,Seattle,98195-2.00,USA. 3 The red cross Transfusion center of hefei,hefei,2.0000,{2.

The understanding of cell injuries in the process of freeze drying is crucial to raise survival rates of living *** reality,the injuries of freeze-dried cells are not all made in the sole process of freeze drying,and some are also made in other processes,including injuries during addition or removal of protective agents as well as injuries caused by *** subdividing the injures into freeze drying ones(only caused in freezing and drying process) and non-freeze-drying ones,the freeze dried damages can be reduced also by eliminating the non-freeze-drying ***,we propose that the non-freeze-drying damages are mainly made by osmotic *** red {2. cells as model,we propose some ways to reduce these damages in the non-freeze-drying processes by properly adjusting the osmotic pressure.

关键词： freeze drying red blood cell rehydration trehalose injury

来源： cnki会议评论

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分析测试学报 2004年第2期23卷 81-83页

作者：周源冯育林太原市红十字血液中心山西太原030024 山西大学现代化学研究所山西太原030006

研究了胶束薄层色谱法测定体液中诺氟沙星(norfloxacin)和氟罗沙星(fleroxacin)含量的方法 ;以聚酰胺层析板为固定相 ,使用0.01mol/L的十二烷基磺酸钠(SDS)水溶液作为展开剂 ,并将EDTA溶液加入到展开剂中避免了氟哌酸 -金属离子络合物... 详细信息

研究了胶束薄层色谱法测定体液中诺氟沙星(norfloxacin)和氟罗沙星(fleroxacin)含量的方法 ;以聚酰胺层析板为固定相 ,使用0.01mol/L的十二烷基磺酸钠(SDS)水溶液作为展开剂 ,并将EDTA溶液加入到展开剂中避免了氟哌酸 -金属离子络合物的生成 ,改善了分离条件 ;在此条件下 ,分离后的NFLX和FLX其Rf值分别为0.62.0.80 ;试样斑点经薄层色谱扫描仪用荧光法扫描定量 ,激发波长为2.8nm(NFLX)和2.5nm(FLX) ;两个化合物的线性范围分别是0～90ng/斑点 (NFLX)和0～80ng/斑点 (FLX) ;回归方程为c=4.96×10-3 A -2.94(NFLX)和c=2.98×10-3 A-3.54(FLX) ,相关系数均在0.998以上 ;在血样和尿样中回收率为98%～102. ,RSD2.。

关键词：体液诺氟沙星氟罗沙星含量测定同时测定胶束薄层荧光法十二烷基磺酸钠

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地市级建立血站的模式思考

天水师范学院学报 1991年第4期11卷 83-85页

作者：乔之宏合肥市红十字中心血站

本文根据国情,用系统方法(System Methool,SM)和功能模拟方法(Function Simulation Methool,FSM)的现代思维方法论的观点提出了快速、节约、高效地建立地市级血站的模式,论证了建立自动运转机制的观点,对建立地市级血站的决策有现实指... 详细信息

本文根据国情,用系统方法(System Methool,SM)和功能模拟方法(Function Simulation Methool,FSM)的现代思维方法论的观点提出了快速、节约、高效地建立地市级血站的模式,论证了建立自动运转机制的观点,对建立地市级血站的决策有现实指导意义,对县血站建立亦具有现实意义.

关键词：血站模式系统方法功能模拟方法

STUDY OF DIELECTRIC PROPERTIES OF HUMANred blood CELL SUSPENSION FROM 2.3HEALTHY PERSONS AT RADIO FREQUENCIES

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Chinese Journal of Biomedical Engineering(English Edition) 1994年第1期3卷 20-27页

作者： Lu Yongjun,Yu Jue,Ren Yinhua(Dept. BME, Institute of Basic Medical Sciences, Beijing, 100005,china)Zhang Zhixin,Sun Yanmei,Zhi Hong,Yuan Qinzhen(blood {2. of Beijing red cross Society,Beijing, 100086,china)

Dielectric properties or kuamn red blood cell (RBC) suspension (ltematocrit 50%) from 2.3 healthy persons (12. males, 12. remales) were measured at 2.Cin the frequency range rrom 1 to 500MHz, and analysed statistically. The measuringsystem controlled by an IBM-PC computer was composed or a network analyzer(HP4195A), coaxial line sensor and a temperature controlling set. The results showedthat the dielectric properties did not depend on either sex or blood group. However, agehad definite erfect. There was a critical age around 49 years when the dielectric data ofhuman RBC suspensions varied signiricantly.

关键词： Human red blood Cell Suspension Cell Suspension Dielectric properties Heaalthy volunteer Biodielectric Measurement

Rapid Identification of Pathogenic Bacteria by means of TwoConservative Gene Loci′ Specific PCR-CE-RFLP

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Journal of Microbiology and Immunology 2003年第1期1卷 38-43页

作者：高鹏张卓然徐维家安万新张晓慧戴兵范艳萍王运铎李萍温杰于卫健Dalian red cross {2. center Dalian 116001 china 高向仪谢凡迪王永海 Dalian Municipal center Hospital Dalian 116033 China Dalian Medical University Dalian 116027China Dalian center of Clinical Laboratory Dalian 116033 China Dalian red cross {2. center Dalian 116001 China Zhejiang Senior Medical School Zhejiang 310052 China Dalian University Affiliated Hospital Dalian 116021 China Zhuanghe No.1 people's Hospital Zhuanghe 116400 China

To establish a rapid identification method for common pathogenic bacteria on the basis of molecular biology and to construct a preliminary Polymerase Chain Reaction-Capillary Electrophoresis - Restriction Fragment Length Polymorphism (PCR-CE-RFLP) database of bacteria isolated from clinical specimens frequently, 183 strains collected from clinical samples belonging to 12.genera and 19 species whose biochemical characterizations corresponded to the typical ones were examined. The genomic DNAs were amplified by two pairs of fluorescence labeled primers aiming at 16S rRNA gene and 16S-2.S rRNA spacer region gene respectively at the same time. PCR products were then digested by restriction endonuclease HaeⅢ incompletely before taking capillary electrophoresis. The results with the PCR-CE-RFLP patterns of 16S rRNA genes were just alike within some genera, but when it comes to 16S-2.S rRNA spacer region genes, each bacterium showed a unique pattern, which can be distinguished from each other easily. It seems that PCR-CE-RFLP patterns of 16S rRNA gene could only be used to classify the bacteria into family level, whereas the data of 16S-2.S rRNA spacer region gene could be utilized to identify the whole microorganisms as precisely as the species level. In spite of the data of the spacer region gene alone can be sufficiently to verify the whole bacteria, we insist that the 16S rRNA gene could be of some assistant in case that there should be lots of families of bacteria, in which some similar ones, with the same RFLP data of 16S-2.S rRNA spacer region gene, may coexist. This study proves that the utility of PCR-CE-RFLP is a convenient, rapid method to identify pathogenic bacteria, and is also a quick diagnosis measure for application to clinical use.

关键词： S rRNA gene 16S-2.S rRNA gene spacer region Polymerase chain reaction Pathogenic bacteria, RFLP

IL-7对PHA-抗CD3 LAK细胞抑制效应的研究

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上海免疫学杂志 2003年第2期23卷 108-110,127页

作者：叶萍程一櫂王艳范亚欣郭连英钱振超大连市红十字血液中心大连116011 大连医科大学大连116027

文章将IL 7与IL 2.,PHA及αCD3联用诱生PHA αCD3LAK细胞 ,应用活细胞计数、MTT和APAAP等方法测定了细胞增殖活性、mIL 2.的表达、外源性IL 2.消耗、细胞毒活性和效应细胞表型等指标 ,并分析比较了IL 7的加入对以上指标的影响。结果表... 详细信息

文章将IL 7与IL 2.,PHA及αCD3联用诱生PHA αCD3LAK细胞 ,应用活细胞计数、MTT和APAAP等方法测定了细胞增殖活性、mIL 2.的表达、外源性IL 2.消耗、细胞毒活性和效应细胞表型等指标 ,并分析比较了IL 7的加入对以上指标的影响。结果表明IL 7的加入使PHA αCD3LAK细胞消耗IL 2.量、mIL 2.表达的量和细胞的增殖能力降低 ,细胞毒活性减弱 ,并伴有CD8+ 细胞百分率的下降。这一结果呈现出的IL 7的抑制作用尚未见报道 ,有进一步研究的价值。

关键词：白细胞介素7 抗CD3单克隆抗体淋巴因子激活的杀伤细胞

L-精氨酸对大鼠妊高征的治疗作用及其机制的探讨

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上海实验动物科学 2003年第3期23卷 148-150页

作者：王忠民王波叶萍山东大学齐鲁医院济南250012 大连市红十字血液中心大连116001

为了研究L-精氨酸治疗孕鼠妊高征效果及机制,利用亚硝基左旋精氨酸甲酯建立孕鼠妊高征模型,并利用L-精氨酸治疗,观察其对孕鼠妊高征治疗效果及一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素(ET)、血管紧张素II(ANGII)、血栓烷(TXA2.、前列环... 详细信息

为了研究L-精氨酸治疗孕鼠妊高征效果及机制,利用亚硝基左旋精氨酸甲酯建立孕鼠妊高征模型,并利用L-精氨酸治疗,观察其对孕鼠妊高征治疗效果及一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素(ET)、血管紧张素II(ANGII)、血栓烷(TXA2.、前列环素(PGI2.等指标的影响。结果发现L-精氨酸可明显改善孕鼠妊高征症状、体征。模型组与对照组、低、高剂量L-精氨酸组比较血清NO、ANGII、PGI2.平、NOS活性均显著降低,而血清ET、TXA2.平明显增高。结果提示NO浓度、NOS活性降低及血管调节因子失衡,可能为妊高征的发病机制;L-精氨酸对大鼠妊高征有明显的治疗作用。这一结果可能为妊高征的预防和治疗提供新的合理方法及手段。

关键词： L-精氨酸大鼠妊高征治疗作用机制一氧化氮一氧化氮合酶