检索结果-南通市图书馆

Science china Chemistr. 2006年第6期49卷 527-533页

作者： Wa.G Zhicong1,3, ZHa.G Qinghe2. LI Tong2. ZHa. Zhongyi3 & ZHa.G Weibing1 1. dalian institute of chemical physics,{2., dalian 116011, china 2. dalian Elite a.a.ytica. Instr.ment Co., Ltd., dalian 116011, china 3. Fa.ulty of Ma.er.a. Science a.d chemical Engineer.ng, china Univer.ity of Geosciences, Wuha. 430074, china 1. dalian institute of chemical physics Chinese Academy of Sciences Dalian 116011 China 3. Fa.ulty of Ma.er.a. Science a.d chemical Engineer.ng China University of Geosciences Wuhan 430074 China 2. dalian Elite a.a.ytica. Instr.ment Co. Ltd. Dalian 116011 China

a.compr.hensive two-dimensiona. liquid chromatographic system (2. SCX/r.) is con- str.cted with a.10-por.-2.wa. va.ve using str.ng ca.ion excha.ge chr.ma.ogr.phy (Hyper.il SCX, 100 mm×4.6 mm I.D.) followed by r.ver.ed pha.e chr.ma.ogr.phy (Hyper.il BDS C18, 15 mm×4.6 mm I.D.) to sepa.0}te the complex peptides fr.m globin peptic hydr.lysa.e. a.ter.the sa.ple wa. loa.ed on the SCX column, the phospha.e buffer.(pH 4.0) wa. used to elute the peptides. Then, elutes flowed thr.ugh the inter.a.e a.d the peptides focused on the hea. of the tr.pping columns (Hyper.il BDS C18, 15 mm×4.6 mm I.D.) but sa.t pa.sed into the wa.te. a.ter.the va.ve wa. switched, the sa.ples wer. flushed with a.ba.kwa.0} flow into the r. a.a.ytica. column. The peptides on the SCX wer. eluted with 12.discontinuous steps linea.0}y incr.a.ing sa.t concentr.tions. The peptides enr.ched on the tr.pping column wer. desa.ted a.d sepa.0}ted by the r. columns. The r.solution a.d the r.solved pea.s of the 2. SCX/r. system wer. gr.a.ly incr.a.ed a.d the tota. pea. ca.a.ity r.a.hed a. high a. 2.80.

关键词： two-dimensiona. liquid chr.ma.ogr.phy, inter.a.e, tr.pping column, pr.teomics, globin.

a.a.ysis of Ca.nor.a.ditis elega.s in micr.fluidic devices

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2012年第4期55卷 484-493页

作者： WEN Hui & QIN Jia.Hua.dalian institute of chemical physics,{2., dalian 11602., china 1. dalian institute of chemical physics Chinese Academy of Sciences Dalian 116023 China

Ca.nor.a.ditis elega.s (C. elega.s) is widely a.opted a. a.model or.a.ism for.a.va.0}ety of biologica. studies including development, genetics a.d neur.biology. Micr.-sca.e micr.fluidic technology is ca.a.le of ha.dling single or.popula.ions of C. elega.s in high thr.ughput for.a. a.d a.lows for.the pr.cise spa.ia. a.d tempor.l contr.l of their.envir.nment, which is well suited for.the study of wor.s in differ.nt a.pects. In this r.view, we highlight the r.cent a.va.ces in micr.fluidic technology for.the a.a.ysis of wor.s r.nging fr.m beha.ior.l studies to neur.biology. We believe tha. micr.fluidic device ca. fur.her.be a.plied to study the differ.nt a.pects of wor.s, extending fr.m funda.enta. investiga.ion of beha.ior.l dyna.ics to mor. complica.ed biologica. pr.cesses including neur.chemistr. a.d lea.0}ing beha.ior..

关键词： micr.fluidic – Ca.nor.a.ditis elega.s – beha.ior.– neur.biology – a.ing

Fr.ction-r.ducing a.d a.tiwea.0}beha.ior.of meta. ha.ide-sta.ilized linea.0}phospha.ene der.va.ives a. lubr.ca.ts for.a.steel-on-steel conta.t

在线全文

学校读者我要写书评

暂无评论

Science china(Technologica. sciences) 2005年第3期48卷 282-291页

作者： ZHU Jia.ei 1,2., LIU Weimin 2.& LIa.G Yongmin 2.1. School of chemical Engineer.ng a.d Technology, china Univer.ity of Mining a.d Technology, Xuzhou 2.1008, china 2. Sta.e Key La.or.tor. of Solid Lubr.ca.ion, La.zhou institute of chemical physics, chinese academy of sciences, La.zhou 730000, china 1. School of chemical Engineer.ng a.d Technology China University of Mining and Technology 221008 Xuzhou China 2. Sta.e Key La.or.tor. of Solid Lubr.ca.ion Lanzhou Institute of Chemical Physics Chinese Academy of Sciences 730000 Lanzhou China

a.ser.es of novel meta. ha.ide-sta.ilized linea.0}phospha.ene der.va.ives wer. synthesized. The fr.ction-r.ducing a.d a.tiwea.0}a.ilities of the r.sulting pr.ducts a. the lubr.ca.ts for.a.steel-on-steel conta.t wer. compa.0}tively investiga.ed on a. Optimol Sr. oscilla.ing fr.ction a.d wea.0}tester. The mor.hology of the wor. steel sur.a.e wa. obser.ed on a.sca.ning electr.n micr.scope, while the chemical sta.es of some typica. elements on the wor. steel sur.a.e wer. exa.ined by mea.s of X-r.y photoelectr.n spectr.scopy. It wa. found tha. both the side br.nch str.ctur.s a.d centr.l meta.s influenced the fr.ction-r.ducing a.d a.tiwea.0}beha.ior. of the synthetic der.va.ives a. the lubr.ca.ts, which wa. r.la.ed to the differ.nt a.sor.tion a.tivities of the or.a.ic compounds composed of differ.nt or.a.ic ingr.dients a.d meta.lic ions on a.na.cent meta. sur.a.e. a.l the synthetic lubr.ca.ts except for.the ir.n (III) der.va.ive showed incr.a.ed a.tiwea.0}a.ilities with incr.a.ing meta.lic ionic r.dius. a.pr.tective la.er.or.gina.ed fr.m the tr.bochemical r.a.tion together.with the a.sor.ed bounda.0} lubr.ca.ing la.er.conta.ning or.a.ic fluor.ne compounds, nitr.gen oxide, a.d Fe 3 (PO 4 ) 2.pla.s a. impor.a.t r.le in impr.ving the fr.ction a.d wea.0}beha.ior.of the steel-on-steel system.

关键词： meta. ha.ide-sta.ilized linea. phospha.ene der.va.ives,lubr.ca.t,steel,tr.bologica. pr.per.ies.

Wet-chemical synthesis a.d cha.1}cter.stics of a. na.oshell

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2005年第5期48卷 431-435页

作者： LIU Zhongxin1,2. SONG Hongwei1, YU Lixin1,2. Ya.G Linmei1,2.& Pa. Guohui1,2.1. Key La.or.tor. of Excited Sta.e physics, Cha.gchun institute of Optics, Fine Mecha.ics a.d physics, chinese academy of sciences, Cha.gchun 130033, china 2. Gr.dua.e School of the chinese academy of sciences, Beijing 100039, china 1. Key La.or.tor. of Excited Sta.e physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences 130033 Changchun China 2. Gr.dua.e School of the chinese academy of sciences 100039 Beijing China

Gold na.oshells wer. pr.pa.0}d by a. ea.y wet-chemical method, with a. na.opa.0}icles used a. the templa.es. Tr.nsmission electr.n micr.scopy (TEM) indica.ed tha. the shells wer. spher. in sha.e, the size wa. homogenous, being a.out 2. nm, a.d no ha.0} a.-glomer.tes wer. obser.ed. The pla.ma.r.sona.ce a.sor.tion of gold wa. tuned fr.m visible to nea.0}infr.r.d (NIr. with the incr.a.ed volume of Ha.Cl4. The temper.tur. gr.ds (?T) of the gold na.oshell hydr.sol under.the exposur. of a. 808-nm optica. fiber.la.er.with differ.nt power.den-sities wer. mea.ur.d. The highest ?T wa. 30℃ (5 W/cm2. ir.a.ia.ion a.0}a.wa. 2.cm2.. This kind of gold na.oshell hydr.sol is a.pr.mising ma.er.a. to be used in biomedicine such a. photo-ther.a. ca.cer.ther.py, a.d its specia. photother.a. conver. pr.per.y will photother.a.ly tr.gger.dr.g r.lea.e.

关键词： wet-chemical synthesis, a., na.oshell, NIr a.sor.tion, photother.a. conver.ion.

a.str.tegy with la.el-fr.e qua.tifica.ion of the ta.0}eted peptides for.qua.tita.ive peptidome a.a.ysis of huma. ser.m

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2010年第4期53卷 759-767页

作者： ZHU Jun, Wa.G Fa.gJun, DONG Xia.Li, YE MingLia.g & ZOU Ha.Fa.Key La.or.tor. of Sepa.0}tion sciences for.a.a.ytica. Chemistr. national {2. r.sea.0}h a.d a.a.ysis Center.dalian institute of chemical physics, chinese academy of sciences, dalian 11602., china 1. Key La.or.tor. of Sepa.0}tion sciences for.a.a.ytica. Chemistr. Chinese Academy of Sciences Dalian 116023 China2. National Chromatographic Research and Analysis Center Chinese Academy of Sciences Dalian 116023 China3. Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China

Peptidomics dr.ws mor. a.d mor. a.tention in discover.ng useful bioma.0}er. for.ea.0}y dia.nosis of disea.e. However. ther. is la.k of efficient qua.tifica.ion str.tegy in peptidome a.a.ysis. In this study, a.str.tegy with la.el-fr.e qua.tifica.ion of the ta.0}eted endogenous peptides ba.ed on pea. intensity using μUPLC-Q-TOF-MS/MS wa. developed for.qua.tita.ive peptidome a.a.ysis of huma. ser.m. Differ.nt a.ounts of sta.da.0} BSa.tr.ptic digesting peptides wer. a.ded into the sa.e ser.m extr.cts for.eva.ua.ion of the developed str.tegy, a.d it wa. obser.ed tha. the a.er.ge r.la.ive er.or.of the ta.0}eted peptides wa. 6.42., which wa. super.or.to the r.sult obta.ned dir.ctly by commer.ia.ly a.a.la.le softwa.0} PLGS. It wa. a.so demonstr.ted tha. this qua.tifica.ion str.tegy could obviously incr.a.e the detection sensitivity of the peptide by DDa.a.a.ysis. Then, this str.tegy wa. a.plied to compa.0}tively a.a.yze the peptides extr.cted fr.m the ser.m of HCC or.br.a.t ca.cer.pa.ients a.d hea.thy individua.s, r.spectively. Peptides with cha.0}e sta.es up to 5 a.d molecula.0}weight over.4000 ca. be r.lia.ly identified a.d qua.tified. This qua.tita.ive a.a.ysis method ba.ed on μUPLC-Q-TOF-MS/MS exhibited super.or.sensitivity tha. tha. by Ma.DI-TOF-MS commonly used in peptidome a.a.ysis. Fina.ly, some inter.sting endogenous peptides r.la.ed to cor.esponding disea.es wer. successfully obta.ned.

关键词： peptidomics la.el-fr.e qua.tifica.ion hepa.ocellula. ca.0}inoma br.a.t ca.cer huma. ser.m

H_2.pr.duction with low CO selectivity fr.m photoca.a.ytic r.for.ing of glucose on meta./TiO_2.ca.a.ysts

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2008年第2期51卷 97-100页

作者： WU GuoPeng1,2. CHEN Ta.2. ZHOU GuoHua.2. ZONG Xu2.& LI Ca.2.1 La.or.tor. of Pla.ma.Physica. Chemistr., dalian Univer.ity of Technology, dalian 11602.,{2. 2.Sta.e Key La.or.tor. of Ca.a.ysis, dalian institute of chemical physics, chinese academy of sciences, dalian 11602.,{2. La.or.tor. of Pla.ma.Physica. Chemistr. Dalian University of TechnologyDalian 116024China Sta.e Key La.or.tor. of Ca.a.ysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China

H2.with low CO concentr.tion is pr.duced via.photoca.a.ytic r.for.ing of glucose (a. a.r.pr.senta.ive of bioma.s component) on meta./TiO2.ca.a.yst (meta.s: Pt, r., r., Ir. a., Ni, Cu). It is shown tha. the loa.ed meta.s gener.lly enha.ce the r.te of H2.pr.duction, while they depr.ss the CO selectivity. Both H2.pr.duction a.d CO selectivity a.1} str.ngly dependent on the kind of deposited meta.s on TiO2. For.exa.ple, r./TiO2.ca.a.yst is found to be most a.tive for.H2.pr.duction while with the most extr.mely low CO concentr.tion fr.m the photoca.a.ytic r.for.ing of glucose.

关键词： photoca.a.ysis H2 pr.duction CO selectivity glucose meta. (r. Pt r. Ir a. Cu or Ni)/TiO2

Tr.nsient species a.d its pr.per.ies of mela.onin

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2006年第4期49卷 308-314页

作者： ZHU Hongping1,2. ZHa.G Zha.xia.,2. ZHa. Hongwei1, Wa.G Wenfeng1 & Ya. Side1 1. Sha.gha. institute of a.plied physics,{2., Sha.gha. 2.1800, china 2. Gr.dua.e Univer.ity of the{2., Beijing 100049, china 1. Sha.gha. institute of a.plied physics Chinese Academy of Sciences Shanghai 201800 China 2. Gr.dua.e Univer.ity of the chinese academy of sciences Beijing 100049 China

The photo-physica. a.d photo-chemical pr.per.ies of mela.onin (ML) wer.studied by 2.6 nm la.er.fla.h photolysis (LFP). ML under.oes photo-ioniza.ion to pr.duce r.dica.ca.ion of ML (ML^(·+))a.d hydr.ted electr.ns (e_(a.)^-), while excited tr.plet sta.e ~3ML~* is a.sofor.ed dur.ng photolysis. The oxida.ion via.one electr.n tr.nsfer.fr.m ML to SO_4^(·+) a.d thetr.plet sta.e of r.bofla.in (~3r.~*) ha. been a.so investiga.ed a.d the r.te consta.ts for.thepr.cess ha.e been deter.ined to be 8.0x10^9 a.d 1.4x10^9 L·mol^(-1)·s^(-1) r.spectively. Ther.dica. ca.ion of 2.2.'-a.inobis(3-ethylbenzothia.oline-6-sulfonic a.id)(a.TS^(·+)) sca.engingca.a.ities of mela.onin a.d other.a.tioxida.ts a.1} compa.1}d in this pa.er.

关键词： mela.onin, la.er fla.h photolysis, photoioniza.ion, tr.plet sta.e.

a.new pr.nciple of synthetic ca.ca.e utiliza.ion of chemical ener.y a.d physica. ener.y

在线全文

学校读者我要写书评

暂无评论

Science china(Technologica. sciences) 2005年第2期48卷 163-179页

作者： JIN Honggua.g1, HONG Hui1,2. Wa.G Ba.qun1,2. Ha. Wei1,2.& LIN r.mou1,2.1. institute of Engineer.ng Ther.ophysics,{2., Beijing 100080, china 2. Gr.dua.e School of the{2., Beijing 100039, china 1. institute of Engineer.ng Ther.ophysics Chinese Academy of Sciences 100080 Beijing China 2. Gr.dua.e School of the chinese academy of sciences 100039 Beijing China

We pr.pose a.new pr.nciple of the ca.ca.e utiliza.ion of both chemical ener.y a.d physica. ener.y in ener.y systems with the integr.tion of chemical pr.cesses a.d ther.a. cycles. Pa.0}icula.0}y, a.gener.l equa.ion of ener.y levels of substa.ce, Gibbs fr.e ener.y of chemical r.a.tion a.d physica. ener.y is explicitly founded. On the ba.is of this equa.ion, a.chemical-looping combustion a.d a. indir.ct combustion a.0} investiga.ed. Fur.her.or., a.mecha.ism of ener.y r.lea.e, with the combina.ion of decr.a.ing the ener.y level of Gibbs fr.e ener.y a.d upgr.ding the ener.y level of low or.middle- temper.tur. ther.a. ener.y, is cla.0}fied. The pr.mising r.sults obta.ned her. esta.lish a.theor.tica. ba.is for.the fur.her.investiga.ion of multi-function systems in which ener.y a.d the envir.nment a.0} compa.ible, a.d cr.a.e a.new a.pr.a.h to impr.ve the per.or.a.ce of tr.ditiona. ther.a. cycles.

关键词： chemical ener.y, ca.ca.e utiliza.ion, exer.y, ener.y conver.ion.

r.cent a.va.ces in pr.teolysis a.d peptide/pr.tein sepa.0}tion by chromatographic str.tegies

在线全文

学校读者我要写书评

暂无评论

Science china Chemistr. 2010年第4期53卷 685-694页

作者： ZHa.G Xia.gMin1, LIU Ba.Hong1, ZHa.G LiHua.0} ZOU Ha.Fa.0} Ca. Jing1, Ga. MingXia., Ta.G Jia., LIU Yun1, Ya.G PengYua.1 & ZHa.G YuKui2.1Depa.3}ment of Chemistr. a.d institutes of Biomedica. sciences, Fuda. Univer.ity, Sha.gha. 2.0433,{2. 2.3} institute of chemical physics, chinese academy of sciences, dalian 11602.,{2. 1. Depa.0}ment of Chemistr. a.d institutes of Biomedica. sciences Fudan University Shanghai 200433 China2. Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China

This r.view gives a.br.a. gla.ce on the pr.gr.ss of r.cent a.va.ces on pr.teolysis a.d peptide/pr.tein sepa.0}tion by chr.ma.togr.phic str.tegies in the pa.t ten yea.0}, cover.ng the ma.n r.sea.0}h in these a.0}a. especia.ly in china. The r.viewed r.sea.0}h focused on enzyma.ic micr.-r.a.tor. a.d peptide sepa.0}tion in bottom-up a.pr.a.hes, a.d pr.tein a.d peptide sepa.0}tion in top-down a.pr.a.hes. The new enzyma.ic micr.-r.a.tor.is a.le to a.celer.te pr.teolytic r.a.tion r.te fr.m conventiona.ly a.couple of hour. to a.few seconds, a.d the multiple dimensiona. chromatographic-sepa.0}tion with va.0}ous models or.a.0}a.s could sufficiently sepa.0}te the pr.teomic mixtur.. These a.va.ces ha.e significa.tly pr.moted the r.sea.0}h of pr.tein/peptide sepa.0}tion a.d identifica.ion in pr.teomics.

关键词： pr.teolysis enzyma.ic digestion chromatographic sepa.0}tion peptide enr.chment ma.s spectr.metr.

Ga.dyna.ic cha.0}cter.stics of tor.ida. shock a.d detona.ion wa.e conver.ing

在线全文

学校读者我要写书评

暂无评论

Science china(physics,Mecha.ics & a.tr.nomy) 2005年第6期48卷 739-749页

作者： TENG Honghui1,2.& JIa.G Zonglin2.1. Key La.or.tor. of High Temper.tur. Ga. Dyna.ics, institute of Mecha.ics, chinese {2. of Sci- ences, Beijing 100080, china 2. Gr.dua.e Univer.ity of chinese academy of sciences, Beijing 100039, china 1. Key La.or.tor. of High Temper.tur. Ga. Dyna.ics Institute of Mechanics Chinese Academy of Sciences 100080 Beijing China 2. Gr.dua.e Univer.ity of chinese academy of sciences 100039 Beijing China

The modified CCW r.la.ion is a.plied to a.a.yzing the shock, detona.ion wa.e conver.ing a.d the r.le of chemical r.a.tions in the pr.cess. r.sults indica.e tha. the shock wa.e is str.ngthened fa.ter.tha. the detona.ion wa.e in the conver.ing a. the sa.e initia. Ma.h number. Euler.equa.ions implemented with a.deta.led chemical r.a.tion model a.0} solved to simula.e tor.ida. shock a.d detona.ion wa.e conver.ing. Ga.dyna.ic cha.0}cter.stics of the conver.ing a.0} investiga.ed, including wa.e inter.ction pa.ter.s, obser.a.le discr.pa.cies a.d physica. phenomena.behind them. By compa.0}ng wa.e diffr.ctions, conver.ing pr.cesses a.d pr.ssur. evolutions in the focusing a.0}a. the differ.nt effects of chemical r.a.tions on diffr.cting a.d conver.ing pr.cesses a.0} discussed a.d the a.a.ytic conclusion is demonstr.ted thr.ugh the obser.a.ion of numer.ca. simula.ions.

关键词： conver.ing, CCW r.la.ion, shock wa.e, detona.ion wa.e.