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A mutation in signal peptide of rat resistin gene inhibits differentiation of 3T3-L1 preadipocytes

Acta Pharmacologica Sinica 2004年第12期25卷 139-145页

作者： Xi-rong GUO2.3, Hai-xia GONG2. Yan-qin GAO4, Li FEI, Yu-hui NI, Rong-hua CHEN Department of Pediatrics, center of Human Functional Genomics, Nanjing Medical University, Nanjing 2.002., {2. 4Department of Pediatrics, the Third Affiliated Hospital of Nanjing Medical University, Yizheng 2.1900, {2. Department of Pediatrics Center of Human Functional Genomics Nanjing Medical University Department of Pediatrics the Third Affiliated Hospital of Nanjing Medical University

AIM: To detect the resistin expression of white adipose tissue in diet-induced obese (DIO) versus diet-resistant (DR)rats, andto investigatethe relationshipof mutatedresistin and 3T3-L1 preadipocytes differentiation. METHODS: RT-PCR and Western Blot were used to detect gene /protein expression. 3T3-L1 cells were cultured, transfected, and induced to differentiation using 0.5 mmol/L 3-isobutyl-1-methyxanthine (MIX), 1 mg/L insulin, and 1 μmol/L dexamethasone. Oil red O staining was applied to detect the degree of preadipocytes differentiation. RESULTS: Expression of resistin mRNA was upregulated in DIO rats and downregulated in DR rats. However, the expression levels varied greatly within the groups. Sequencing of the resistin genes from DIO and DR rats revealed a Leu9Val (C2.G) missense mutation within the signal peptide in one DR rat. The mutant resistin inhibited preadipocyte differentiation. Local experiments and Western blotting with tagged resistin fusion proteins identified both mutant and wild type proteins in the cytoplasm and secreted into the culture medium. Computer predictions using the Proscan and Subloc programs revealed four putative phosphorylation sites and a possible leucine zipper motif within the rat resistin protein. CONCLUSION: Resistin-increased differentiation may be inhibited by the mutation- containing precursor protein, or by the mutant non-secretory resistin isoform.

关键词： resistin mutation adipocyte differentiation obesity

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Biomimetic natural biomaterials for tissue engineering and regenerative medicine:new biosynthesis methods,recent advances,and emerging applications

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Military Medical Research 2024年第1期11卷 50-79页

作者： Shuai liu Jiang-Ming Yu Yan-Chang Gan Xiao-Zhong Qiu Zhe-Chen Gao Huan Wang Shi-Xuan Chen Yuan Xiong Guo-hui liu Si-En Lin Alec McCarthy Johnson V.John Dai-Xu Wei Hong-Hao Hou Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering the Fifth Affiliated HospitalSchool of Basic Medical ScienceSouthern Medical UniversityGuangzhou 510900China Department of Orthopedics Tongren HospitalShanghai Jiao Tong UniversityShanghai 200336China The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen 518033GuangdongChina Engineering Research center of Clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325011ZhejiangChina Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Orthopaedics and Traumatology Faculty of Medicinethe Chinese University of Hong KongHong Kong SAR 999077China Department of Functional Materials Terasaki Institute for Biomedical InnovationLos AngelesCA 90064USA Mary&Dick Holland Regenerative Medicine Program College of MedicineUniversity of Nebraska Medical CenterOmahaNE 68130USA Zigong Affiliated Hospital of Southwest Medical University Zigong Psychiatric Research CenterZigong Institute of Brain ScienceZigong 643002SichuanChina Key Laboratory of Resource Biology and Biotechnology in Western china Ministry of EducationSchool of MedicineDepartment of Life Sciences and MedicineNorthwest UniversityXi’an 710127China

Biomimetic materials have emerged as attractive and competitive alternatives for tissue engineering(TE)and regenerative *** contrast to conventional biomaterials or synthetic materials,biomimetic scaffolds based on natural biomaterial can offer cells a broad spectrum of biochemical and biophysical cues that mimic the in vivo extracellular matrix(ECM).Additionally,such materials have mechanical adaptability,micro-structure interconnectivity,and inherent bioactivity,making them ideal for the design of living implants for specific applications in TE and regenerative *** paper provides an overview for recent progress of biomimetic natural biomaterials(BNBMs),including advances in their preparation,functionality,potential applications and future *** highlight recent advances in the fabrication of BNBMs and outline general strategies for functionalizing and tailoring the BNBMs with various biological and physicochemical characteristics of native ***,we offer an overview of recent key advances in the functionalization and applications of versatile BNBMs for TE ***,we conclude by offering our perspective on open challenges and future developments in this rapidly-evolving field.

关键词： Biomimic Scaffold Biosynthesis Natural biomaterial Tissue engineering

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超声骨刀在根管外科中应用的研究进展

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国际口腔医学杂志 2016年第6期43卷 672-676页

作者：陈瑶申静南开大学医学院口腔医学系天津300071 天津市口腔医院国际诊疗中心天津300041

超声骨刀手术系统是指利用压电超声频率微振荡刀进行无损伤骨组织的一种新型的骨切割手术设备,可替代传统口腔手术中的机电设备。超声骨刀在口腔领域广泛应用,主要包括口腔颁面外科手术、上颌窦提升术,牙周手术等。近年来,超声骨刀也用... 详细信息

超声骨刀手术系统是指利用压电超声频率微振荡刀进行无损伤骨组织的一种新型的骨切割手术设备,可替代传统口腔手术中的机电设备。超声骨刀在口腔领域广泛应用,主要包括口腔颁面外科手术、上颌窦提升术,牙周手术等。近年来,超声骨刀也用于根管外科,其优势是降低了软组织损伤的风险,提供了清晰的手术视野且切割产热少。本文主要就超声骨刀手术系统的工作原理以及在根管外科中的应用作一综述。

关键词：根管外科根尖手术超声骨刀超声压电设备

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Effect of aerosols on visibility and radiation in tianjin,china

Effect of aerosols on visibility and radiation in Tianjin,Ch...

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强化科技基础推进气象现代化——第2.届中国气象学会年会

作者： Suqin Han 1,Hai Bian 1,Yufen Zhang 2.Jianhui Wu 2.Yimei Wang 2.Xuexi Tie 3,Yinghua Li 1 Xiangjin Li 1 Qing Yao 1 1 tianjin Institute of meteorological science,tianjin,300074 2.nankai University,tianjin,300071.3 National center for Atmospheric Research,Boulder,CO USA

Meteorological and aerosol data were measured at the atmospheric boundary layer observation station in tianjin,china,and were analyzed to study the effects of aerosol mass,composition,and size distributions on visibility and short-term radiation *** results show that fine particles played important roles in controlling visibility in *** major contributors to light extinction coefficients included sulfate(2..7%),particulate organic matter(2..6%),elemental carbon(19.2.),and nitrate(6.1%).In addition to the measurement of aerosol composition,the size distribution of aerosol number concentrations were also measured and classified between haze days and non-haze days during *** extinction characteristics of ambient aerosol in haze days and non-haze days were calculated using Mie theory *** average extinction coefficient and scattering coefficient of atmospheric aerosols were 0.2.3 km-1 and 0.2.3 km-1 in non-haze days,while 0.767 km-1 and 0.665 km-1 in haze days.A radiation transmission model LOWTRAN7 is also {2. in this *** model calculated radiant flux densities in haze days and non-haze days,which showed a fairly agreement with the observation results,showing that the heavy aerosol loadings in tianjin had significantly impact on atmospheric visibility and radiation fluxes.

关键词： aerosol visibility extinction coefficient refractive index radiation fluxes

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The Study on Produce and Properties ofF-Heparin Surface Modified IOLs

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Chinese Journal of Biomedical Engineering(English Edition) 2003年第1期12卷 1-16页

作者： GU Han qing 1,YUAN Jia qin 2.SUN hui min 2.ZHANG Hai wen 1,LU Mo gu 1,WANG Gui qin 3,GUO Hong yu 3,XU Yan shan 2.his work is funded in part by the National 10th Five Year Plan on Capture Key of Science and Technolog (2.01 BA706B17) 1 tianjin Institute of Urological Surgery,tianjin 3002.1,china,2.tianjin Medical University Eye center,tianjin 300070,china 3 tianjin Medical University,Tiangjin 300070,china

Objective:To study the biocompatibility of F heparin surface modified IOLs (F HSM IOLs) and provide theoretical and experimental basis for clinical use and new IOL development. Methods The technique of carrier energy ion beam combined with low temperature and low pressure plasma was used to modify the surface of PMMA IOLs. The physicochemical and optical properties of F HSM IOLs were examined. In vitro, the cultured cells adherence and growth on IOLs were studied. In vivo, F HSM IOLs were implanted into rabbits and Rhesus Monkeys eyes. The biocompatibility of F HSM IOLs, PMMA IOLs and HSM IOLs was compared. Results There were fewer adhered cells on the surface of F HSM IOLs with no toxicity to the cultured fibroblasts. F HSM IOLs caused less inflammatory reaction, broke BAB more slightly and decreased posterior capsular opacity. F HSM IOLs possessed better biocompatiblity. Our research also suggested that the brownish F HSM IOLs could prevent the short wave length light and ultraviolet rays from injuring the retina. Conclusion F HSM IOLs possessed better biocompatibility than conventional PMMA IOLs and can be used in clinical works after further inspection and approval.

关键词： F heparin surface modified IOLs Biocompatibility Preparation In vitro experiments In vivo experiments Rabbits Rhesus Monkeys

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Characteristics and genesis of groundwater salinization in coastal areas of the Lower Reaches of Oujiang Basin

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Journal of Groundwater Science and Engineering 2024年第2期12卷 190-204页

作者： Mei-hui Zhang Shi-yang Zhou Dan-dan liu Ying Zhang Yu-xi Zhang Xi Chen hui-wei Wang Bei Li Wei Kang Bing Yi Wan-peng Shi Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuang 050061China Fujian Provincial Key Laboratory of Water Cycling and Eco–Geological Processes Xiamen 361021FujianChina china University of Geosciences(Beijing) Beijing 100083China Changsha center of Natural Resources Comprehensive Survey China Geological SurveyChangsha 410600China Hydrogeological Environmental Geological Survey center of china Geological Survey Tianjin 071501China china University of Geosciences Wuhan 430074China

The coastal areas of the lower reaches of Oujiang River Basin are rich in groundwater ***,the unsustainable exploitation and utilization of groundwater have led to significant changes in the groundwater *** the characteristics and genesis of groundwater salinization is crucial for preventing its deterioration and ensuring sustainable *** this study,a comprehensive approach combining the ion ratio method,mineral saturation index method and multivariate statistical analysis was employed to investigate the hydrochemical characteristics and main controlling factors in the study *** findings reveal that:(1)Groundwater samples in study area exhibit a neutral to slightly alkaline *** predominant chemical types of unconfined water are HCO_(3)-Ca·Na,HCO_(3)·Cl-Na·Ca and HCO_(3)·SO_(4)-Ca·Na,while confined water mainly exhibits Cl·HCO_(3)-Na and Cl-Na types.(2.Salinity coefficients indicate an increase in salinity from unconfined to confined ***,Na^(+)and Cl^(–)concentrations show an increasing trend from mountainous to coastal areas in unconfined water,while confined water displays variability in TDS,Na^(+)and Cl^(–)concentrations.(3)Groundwater salinity is mainly influenced by water-rock interactions,including the dissolution of halite and gypsum,cation exchange,and seawater intrusion ***,human activities and carbonate dissolution contribute to salinity in unconfined *** intrusion is identified as the primary factor leading to higher salinity in confined water compared to unconfined water,with increasing cation exchange and seawater interaction observed from unconfined to confined water.

关键词： Hydrochemical Multivariate statistical analysis Seawater intrusion

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Spatial Distribution Feature Extraction Network for Open Set Recognition of Electromagnetic Signal

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Computer Modeling in Engineering & Sciences 2024年第4期139卷 279-296页

作者： hui Zhang Huaji Zhou Li Wang Feng Zhou The Ministry Key Laboratory of Electronic Information Countermeasure and Simulation Xidian UniversityXi’an710071China Science and Technology on Communication Information Security Control Laboratory Jiaxing314033China

This paper proposes a novel open set recognition method,the Spatial Distribution Feature Extraction Network(SDFEN),to address the problem of electromagnetic signal recognition in an open *** spatial distribution feature extraction layer in SDFEN replaces convolutional output neural networks with the spatial distribution features that focus more on inter-sample information by incorporating class center *** designed hybrid loss function considers both intra-class distance and inter-class distance,thereby enhancing the similarity among samples of the same class and increasing the dissimilarity between samples of different classes during ***,this method allows unknown classes to occupy a larger space in the feature *** reduces the possibility of overlap with known class samples and makes the boundaries between known and unknown samples more ***,the feature comparator threshold can be used to reject unknown *** signal open set recognition,seven methods,including the proposed method,are applied to two kinds of electromagnetic signal data:modulation signal and real-world *** experimental results demonstrate that the proposed method outperforms the other six methods overall in a simulated open ***,compared to the state-of-the-art Openmax method,the novel method achieves up to 8.87%and 5.2.%higher micro-F-measures,respectively.

关键词： Electromagnetic signal recognition deep learning feature extraction open set recognition

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CAH-X综合征研究进展

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中华实用儿科临床杂志 2024年第8期39卷 628-633页

作者：李震刘晖福建医科大学妇儿临床医学院福建省儿童医院(上海儿童医学中心福建医院)内分泌遗传代谢科福州350014

CAH-X综合征是指先天性肾上腺皮质增生症(CAH)患者中,合并肌腱蛋白X(TNX)缺陷而出现埃勒斯-当洛综合征表型的特殊亚群,占CAH患者的10%~15%。TNX缺陷可导致一系列结缔组织症状,包括全身性关节活动过度、皮肤过度伸展、反复关节脱位、慢... 详细信息

CAH-X综合征是指先天性肾上腺皮质增生症(CAH)患者中,合并肌腱蛋白X(TNX)缺陷而出现埃勒斯-当洛综合征表型的特殊亚群,占CAH患者的10%~15%。TNX缺陷可导致一系列结缔组织症状,包括全身性关节活动过度、皮肤过度伸展、反复关节脱位、慢性疼痛、心脏缺陷等,严重影响患者生存质量。CAH-X综合征的遗传学病因是CYP2.A2.TNXB基因的连续性缺陷,由于致病基因的复杂性,其分子诊断充满挑战。现对CAH-X综合征研究进展进行综述,以提高临床医师对于这一新发现疾病的认识。

关键词：先天性肾上腺皮质增生症 CAH-X综合征埃勒斯-当洛综合征 TNXB基因

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Digitalizing river aquatic ecosystems

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Journal of Environmental Sciences 2024年第3期137卷 677-680页

作者： Yaohui Bai hui Lin Chenchen Wang Qiaojuan Wang Jiuhui Qu Key Laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China School of Environmental and Municipal Engineering Tianjin Chengjian UniversityTianjin 300384China center for Water and Ecology Tsinghua UniversityBeijing 100084China

Traditional river health assessment relies on limited water quality indices and representative organism activity,but does not comprehensively obtain biotic and abiotic information of the ***,we propose a new approach to evaluate the ecological and health risks of river aquatic ***,detailed physicochemical and biological characterization of a river ecosystem can be obtained through pollutant determination(especially emerging pollutants)and DNA/RNA ***,supervised machine learning can be applied to perform classification analysis of characterization data and ascertain river ecosystem ecology and *** proposed methodology transforms river ecosystem health assessment and can be applied in river management.

关键词： Digitalizing River ecosystem health Emerging pollutants High throughput sequencing Machine learning

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Electromagnetic fields in ultra-peripheral relativistic heavy-ion collisions

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Nuclear Science and Techniques 2024年第2期35卷 103-109页

作者： Jie Zhao Jin-hui Chen Xu-Guang Huang Yu-Gang Ma Key Laboratory of Nuclear Physics and Ion-beam Application(MOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Shanghai Research center for Theoretical Nuclear Physics NSFC and Fudan UniversityShanghai 200438China Physics Department and center for Particle Physics and Field Theory Fudan UniversityShanghai 200438China

Ultra-peripheral heavy-ion collisions(UPCs)offer unique opportunities to study processes under strong electromagnetic *** these collisions,highly charged fast-moving ions carry strong electromagnetic fields that can be effectively treated as photon *** exchange of photons can induce photonuclear and two-photon interactions and excite *** excitation of the ions results in Coulomb dissociation with the emission of photons,neutrons,and other ***,the electromagnetic fields generated by the ions can be sufficiently strong to enforce mutual interactions between the two colliding ***,the two colliding ions experience an electromagnetic force that pushes them in opposite directions,causing a back-to-back correlation in the emitted *** a Monte Carlo simulation,we qualitatively demonstrate that the above electromagnetic effect is large enough to be observed in UPCs,which would provide a clear means to study strong electromagnetic fields and their effects.

关键词： Electromagnetic fields Neutrons Ultra-peripheral relativistic heavy-ion collisions(UPC)

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