检索结果-南通市图书馆

Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

Chinese science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2.3,4, WANG Kemin1,2.3,4, XIAO Zhiqiang5,6, WANG Wei1,3,4, TAN Weihong1,2.3,4, SUN Yi5,6, TANG Hongxing1,3,4 & YANG Xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan university, chang{2. 410082, china 2. life science and biotechnology institute, hunan university, chang- sha 410082, china 3. Chemistry and Chemical Engineer College, hunan university, chang{2. 410082, china 4. hunan Engineering Research Center for Bio-Nanotechnology, hunan university, chang{2. 410082, china 5. Medical Experiment Research Center, Xiangya Hospital, Central South university, chang{2. 410008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, chang{2. 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China life science and {2. institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China hunan Engineering Research Center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research Center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Efficient electroreduction of nitrate via enriched active phases on copper-cobalt oxides

引用

Chinese Chemical Letters 2024年第4期35卷 204-209页

作者： Ling Fang sha Wang Shun Lu Fengjun Yin Yujie Dai Lin chang Hong Liu Chongqing institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714China university of Chinese Academy of sciences Beijing 100049China

Electrochemical conversion of nitrate(NO_(3)~-) to ammonia(NH_(3)) can target two birds with one stone well, in NO_(3)^(-)-containing sewage remediation and sustainable NH_(3) production. However, single metalbased catalysts are difficult to drive high-efficient NO_(3)~- removal due to the multi-electron transfer ***, we present a tandem catalyst with simple structure, Cu-Co binary metal oxides(Cu-Co-O), by engineering intermediate phases as catalytic active species for NO_(3)~- conversion. Electrochemical evaluation,X-ray photoelectron spectroscopy, and in situ Raman spectra together suggest that the newly-generated Cu-based phases was prone to NO_(3)~- to NO_(2.~- conversion, then NO_(2.~- was reduced to NH_(3) on Co-based species. At an applied potential of -1.1 V vs. saturated calomel electrode, the Cu-Co-O catalyst achieved NO_(3)~- -N removal of 90% and NH_(3) faradaic efficiency of 81% for 12. min in 100 m L of 50 mg/L NO_(3)~- -N,consuming only 0.69 k Wh/mol in a two-electrode system. This study provides a facile and efficient engineering strategy for developing high-performance catalysts for electrocatalytic nitrate conversion.

关键词： NO_(3)^(-)-containing sewage remediation Electrocatalytic reduction Copper-cobalt oxides Tandem reactivity Enriched active phases

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Practical Synthesis of Valbenazine via 1,3-Dipolar Cycloaddition

引用

Chinese Journal of Chemistry 2024年第8期42卷 841-845页

作者： Yalan Peng Zuming Lin Lili Zhu Shiqing Han sha-Hua Huang Ran Hong College of biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjingJiangsu211816 China School of Environmental and Chemical Engineering Shanghai Institute of TechnologyShanghai201418 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032 China Innovation Research institute of Traditional Chinese Medicine(IRI) Shanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai201203 China

Comprehensive Summary,Valbenazine(Ingrezza),a potent and highly selective inhibitor of vesicular monoamine transporter type 2.VMAT2.through the active metabolite hydrotetrabenazine(HTBZ),has been approved for the treatment of tardive dyskinesia and,very recently,for chorea,which is associated with Huntington's *** numerous synthetic efforts dedicated to the synthesis of HTBZ,the industrial preparation of valbenazine uses dihydroisoquinoline as a starting material and the chiral resolution of racemic HTBZ derived from ketone ***,we present a practical synthesis of HTBZ and valbenazine featuring a highly stereoselective 1,3-dipolar cycloaddition and enzymatic kinetic *** cascade process includes cycloaddition,N—O bond cleavage,and lactamization,which proved to be operationally *** allure of the enzymatic resolution developed in this work offers a rapid access toward affording tetrahydroisoquinoline(THIQ)-fused piperidine in the production of medically significant compounds,such as yohimbine and reserpine.

关键词： Cycloaddition Chemoenzymatic Kinetic resolution Prodrugs VMAT2.inhibitor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Waterlogged and Aerobic Incubation on Enzyme Activities in Paddy Soil

引用

Pedosphere 2006年第4期16卷 532-539页

作者： WANG Xiao-chang LU Qin Department of Tea science College of Agriculture and Biotechnology Zhejiang University Hangzhou 31002g （China） institute of Soil science Chinese Academy of Sciences Nanjing 210008 （China）

An incubation experiment with soil water content treatments of 0.15 （W1）, 0.2. （W2., and 0.40 （W3） g g^-1 soil was carried out for two months to investigate the activities of important enzymes involved in C, N, P, and S cycling in a paddy soil from the Taihu Lake region, china, under waterlogged and aerobic conditions. Compared with air-dried soil, waterlogging resulted in a significant decrease （P ≤ 0.05） of fluorescein diacetate （FDA） and /3-D-glucosidase activities, and this effect was enhanced with increasing waterlogging time. Waterlogging also significantly inhibited （P ≤ 0.05） arylsulfatase as well as alkaline and acid phosphatase activities, but did not decrease the activities with the increase in waterlogging time. Short-term waterlogging did not affect urease activity, but prolonged waterlogging decreased it markedly. In contrast, the aerobic incubation （W1 and W2.treatments） significantly increased （P ≤ 0.05） FDA, alkaline phosphatase, and /3-D-glucosidase activities. With aerobic treatments the activities of FDA and alkaline phosphatase increased with incubation time, whereas /3-D-glucosidase activity decreased. A significant difference （P ≤ 0.05） was usually observed between the W1 and W2.treatments for the activities of FDA as well as alkaline and acid phosphatase; however,/3-D-glucosidase and urease were usually not significant （P ≤ 0.05）. No activity differences were observed between waterlogging and aerobic incubation for arylsulfatase and urease.

关键词： aerobic incubation enzyme activity paddy soil waterlogging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Purification and characterization of a novel cholesterol-lowering protein from the seeds of Senna obtusifolia

引用

science china({2. sciences) 2008年第11期51卷 1020-1024页

作者： LI ChuHua1, LI Mei2. chang WenRui2.& GUO BaoJiang1 1 College of life {2.s, South china Normal university, Guangdong Provincial Key Lab of biotechnology of Plant Development, Guang-zhou 510631, china 2.institute of Biophysics, Chinese Academy of {2.s, Beijing 100101, china College of life sciences South China Normal University Guangdong Provincial Key Lab of Biotechnology of Plant Development Guangzhou China institute of Biophysics Chinese Academy of Sciences Beijing China

"Juemingzi", a source of traditional Chinese herbal medicine, has been demonstrated to play a role in decreasing serum cholesterol concentration. In this study, a novel protein, which has shown an in-hibitory effect on cholesterol biosynthesis, was isolated from Senna obtusifolia L. seed by gel filtration and ion exchange chromatography. The novel protein’s molecular mass was 19.7 kD and its pI was 4.80. Both SDS-PAGE and isoelectric-focusing (IEF) revealed a single Coomassie brilliant blue stained band, indicating that the novel protein was a single peptide. The N-terminal amino acid sequence of the pro-tein was IPYISASFPLNIEFLPSE, which had no similarity with any other protein sequences in the NCBI protein database. Circular dichroism (CD) signals indicated that S. obtusifolia seed protein contained 12.5% α-helix, 55.6% β-sheet, and 31.9% random coil.

关键词： Senna obtusifolia L. seed novel protein purification biochemical properties cholesterol biosynthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

引用

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2. chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3) 1 Key Laboratory of Tea Biochemistry and biotechnology,Ministry of Agriculture,Anhui Agricultural {2.,Hefei 2.0036,china 2.College Of Horticulture,Nanjing Agriculture {2.,Nanjing 2.0095,china 3 Auhui institute of Education,Hefei 2.0036,china Key Laboratory of Tea Biochemistry and biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China College Of Horticulture Nanjing Agriculture UniversityNanjing 210095 China Auhui institute of EducationHefei 2.0036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32.expression system and expressed in Escherichia coli BL2.trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Ionic Liquid-Enhanced Assembly of Nanomaterials for Highly Stable Flexible Transparent Electrodes

引用

Nano-Micro Letters 2024年第7期16卷 441-455页

作者： Jianmin Yang Li chang Xiqi Zhang Ziquan Cao Lei Jiang Key Laboratory of Bio-Inspired Materials and Interfacial science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China university of Chinese Academy of sciences Beijing 100049People’s Republic of China College of Chemistry and Chemical Engineering Chongqing University of TechnologyChongqing 400054People’s Republic of China Binzhou institute of Technology Binzhou 256600People’s Republic of China Nanomics biotechnology Co. Ltd.HangzhouPeople’s Republic of China

The controlled assembly of nanomaterials has demon-strated significant potential in advancing technological ***,achieving highly efficient and low-loss assembly technique for nanomate-rials,enabling the creation of hierarchical structures with distinctive func-tionalities,remains a formidable ***,we present a method for nanomaterial assembly enhanced by ionic liquids,which enables the fabrication of highly stable,flexible,and transparent electrodes featuring an organized layered *** utilization of hydrophobic and non-volatile ionic liquids facilitates the production of stable interfaces with water,effectively preventing the sedimentation of 1D/2. nanomaterials assembled at the ***,the interfacially assembled nanomaterial monolayer exhibits an alternate self-climbing behavior,enabling layer-by-layer transfer and the formation of a well-ordered MXene-wrapped silver nanowire network *** resulting composite film not only demonstrates exceptional photoelectric performance with a sheet resistance of 9.4Ωsq^(-1) and 93%transmittance,but also showcases remarkable environmental stability and mechanical *** noteworthy is its application in transparent electromagnetic interference shielding materials and triboelectric nanogenerator *** research introduces an innovative approach to manufacture and tailor functional devices based on ordered nanomaterials.

关键词： Ionic liquids Assembly Silver nanowires MXene nanosheets Flexible transparent electrodes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Establishment of a transformation system in close relatives of wheat under the assistance of TaWOX5

引用

Journal of Integrative Agriculture 2024年第6期23卷 1839-1849页

作者： Yanan chang Junxian Liu chang Liu Huiyun Liu Huali Tang Yuliang Qiu Zhi{2.n Lin Ke Wang Yueming Yan Xingguo Ye institute of Crop sciences Chinese Academy of Agricultural SciencesBeijing 100081China Beijing Key Laboratory of Plant Gene Resources and biotechnology for Carbon Reduction and Environment Improvement/College of life {2. Capital Normal UniversityBeijing 100048China College of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome Engineering/institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

Species closely related to wheat are important genetic resources for agricultural production,functional genomics studies and wheat *** this study,a wheat gene related to regeneration,TaWOX5,was applied to establish the Agrobacterium-mediated transformation systems of Triticum monococcum,hexaploid triticale,and rye(Secale cereale L.)using their immature *** plants were efficiently *** the transformation process,the Agrobacterium infection efficiency was assessed by histochemical staining forβ-glucuronidase(GUS).Finally,the transgenic nature of regenerated plants was verified by polymerase chain reaction(PCR)-based genotyping for the presence of the GUS and bialaphos resistance(bar)genes,histochemical staining for GUS protein,and the QuickStix strip assay for bar *** transformation efficiency of *** genotype PI42.182.was 94.4%;the efficiencies of four hexaploid triticale genotypes Lin456,ZS32.7,ZS12.7,and ZS32.4 were 52.1,41.2.19.4,and 16.0%,respectively;and the transformation efficiency of rye cultivar Lanzhou Heimai was 7.8%.Fluorescence in situ hybridization(FISH)and genomic in situ hybridization(GISH)analyses indicated that the GUS transgenes were integrated into the distal or near centromere(proximal)regions of the chromosomes in transgenic *** and hexaploid triticale *** the transgenic hexaploid triticale plants,the foreign DNA fragment was randomly integrated into the AABB and RR ***,the transgene was almost stably inherited in the next generation by Mendel’s *** findings in this study will promote the genetic improvement of the three plant species for grain or forage production and the improvement of cereal species including wheat for functional genomics studies.

关键词： Triticum monococcum hexaploid triticale rye TaWOX5 Agrobacterium transformation efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nonuniform stretch processing for the range profile of a target with micro-motion

引用

Progress in Natural science:Materials International 2006年第11期16卷 1205-1213页

作者： CHEN Hangyong**, JIANG Weidong, LIU Yongxiang, LI Xiang and GUO Guirong(institute of Space Electronics Information Technology, National university of Defense Technology, {2. sha 410073, china) institute of Space Electronics Information Technology National University of Defense Technology Chang Sha 410073 China

Complex motions such as micro-motions induce complex range-Doppler coupling effect in radar imaging. Hence, it is a challenge to reconstruct a clear range profile of a target with micro-motion. Stretch processing with fine motion compensation cannot perform well while it ties to eliminate the distortion of range profile caused by Doppler effect, because it is difficult to accurately estimate each scattering center velocity during coherent processing interval. Based on nonuniform stretch processing (NSP), a new technology is proposed to decouple range, time and Doppler and then achieve range profile of target with micro-motion. The new technology uses a new synthetic wide-band waveform and has an innovative mathematic interpretation. Statistical properties of the new approach are primarily examined. Typical models of target with micro-motion for high resolution radar are presented. Monte Carlo simulations demonstrate the performance of the new technology and the simulation results confirm our expectations.

关键词： synthetic range profile micro-motion stretch processing Doppler effect.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synergistic PM_(2.5)and O_(3)control to address the emerging global PM_(2.5)-O_(3)compound pollution challenges

引用

Eco-Environment & Health 2024年第3期3卷 325-337页

作者： Chao He Jianhua Liu Yiqi Zhou Jingwei Zhou Lu Zhang Yifei Wang Lu Liu sha Peng College of Resources and Environment Yangtze UniversityWuhan 430100China Hubei Key Laboratory of Petroleum Geochemistry and Environment Yangtze UniversityWuhan 430100China School of Geography and Ocean science Nanjing UniversityNanjing 210023China Hydrology and Environmental Hydraulics Group Wageningen University and ResearchWageningen 6700 HBthe Netherlands State Key Laboratory of Freshwater Ecology and biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China State Key Joint Laboratory for Environmental Simulation and Pollution Control School of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China State Key Laboratory of Pollution Control and Resource Reuse School of EnvironmentNanjing UniversityNanjing 210023China Collaborative Innovation Center for Emissions Trading System Co-constructed by the Province and Ministry Hubei University of EconomicsWuhan 430205China

In recent years,the issue of PM_(2.5)-O_(3)compound pollution has become a significant global environmental *** study examines the spatial and temporal patterns of global PM_(2.5)-O_(3)compound pollution and exposure risks,firstly at the global and urban scale,using spatial statistical regression,exposure risk assessment,and trend analyses based on the datasets of daily PM_(2.5)and surface O_(3)concentrations monitored in 12. cities around the world from 2.19 to ***,on the basis of the common emission sources,spatial heterogeneity,interacting chemical mechanisms,and synergistic exposure risk levels between PM_(2.5)and O_(3)pollution,we proposed a synergistic PM_(2.5)-O_(3)control framework for the joint control of PM_(2.5)and *** results indicated that:(1)Nearly 50%of cities worldwide were affected by PM_(2.5)-O_(3)compound pollution,with china,South Korea,Japan,and India being the global hotspots for PM2.5-O3 compound pollution;(2.Cities with PM_(2.5)-O_(3)compound pollution have exposure risk levels dominated by ST t ST(Stabilization)and ST t HR(High Risk).Exposure risk levels of compound pollution in developing countries are significantly higher than those in developed countries,with unequal exposure characteristics;(3)The selected cities showed significant positive spatial correlations between PM_(2.5)and O_(3)concentrations,which were consistent with the spatial distribution of the precursors NOx and VOCs;(4)During the study period,52.5%of cities worldwide achieved synergistic reductions in annual average PM_(2.5)and O_(3)*** average PM_(2.5)concentration in these cities decreased by 13.97%,while the average O_(3)concentration decreased by 19.18%.This new solution offers the opportunity to construct intelligent and healthy cities in the upcoming low–carbon transition.

关键词： PM_(2.5)-O_(3)compound pollution Population exposure risk Spatial correlation Synergistic treatment potential

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：