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science china({2. sciences) 2022年第5期65卷 1046-1049页

作者： Pan Gong Siwen Zhao Hui Liu Zhaoyang chang Fangfang Li Xueping Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Dear Editor,Plant viruses spread from the initially infected cells to adjacent cells through plasmodesmata(PD) to complete their successful infections in host *** cell-to-cell movement requires specific viral protein(s),known as movement protein(s)(MP).Generally,virus MPs can bind to nucleic acids and modify PD size exclusion limit(Schoelz et al.,2.11).Geminiviruses are DNA viruses that cause devastating losses to food crops worldwide,and encode 4–8 classical viral proteins(Gong et al.,2.2.;Hu et al.,2.2..

关键词： movement yellow

Structure and Quantum Chemistry Study on Hexaacetyl D-Mannose Hydrazine

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Chinese Journal of Structural Chemistry 2007年第8期26卷 911-916页

作者： CAO Rui LIU Xiao-Hong CHENG chang-Mei WANG Ru-Ji ZHAO Yu-Fen WANG Lai-Xi Key Laboratory of Bioorganic Phosphorus Chemistry ＆ Chemical Biology （Ministry of Education） Department of Chemistry Tsinghua University Beijing 100084 China institute of Human Virology university of Maryland {2. institute university of Maryland USA

Hexaacetyl D-mannose hydrazine is one type of important intermediates in saccharide chemistry. In this paper, its single crystal was obtained and furthermore, X-ray diffraction and quantum chemistry calculation were performed. It belongs to orthorhombic system, space group P2.2.2., with a=16.2.7（3）, b=19.2.3（3）, c=7.1948（12.A, Mr=446.41, Dc=1.315 g/cm^3, V=2.54.5（6）A^3 and Z=4. Meanwhile, the experimental results also provide information for designing a kind of molecular switch based on the mannose nitrogenous derivatives.

关键词： hexaaeetyl D-mannose hydrazine crystal structure calculation of quantum chemistry molecular switch

Phytochromes Regulate SA and JA Signaling Pathways in Rice and Are Required for Developmentally Controlled Resistance to Magnaporthe grisea

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Molecular Plant 2011年第4期4卷 688-696页

作者： Xian-Zhi Xie Yan-Jiu Xue Jin-Jun Zhou Bin Zhang Hong chang Makoto Takano High-Tech Research Center Shandong Academy of Agricultural Sciences Jinan 250100 PR China Laboratory of Crop Genetic Improvement and biotechnology Huanghuaihai Ministry of Agriculture Jinan 250100 PR China College of life science Shanxi Agricultural University TaiguShanxi 030801 PR China National institute of Agrobiological sciences Tsukuba Ibaraki 305-8602 Japan

Old leaves of wild-type rice plants （Oryza sativa L. cv. Nipponbare） are more resistant to blast fungus （Magnaporthe grisea） than new leaves. In contrast, both old and new leaves of the rice phytochrome triple mutant （phyAphyBphyC） are susceptible to blast fungus. We demonstrate that pathogenesis-related class 1 （PR1） proteins are rapidly and strongly induced during M. grisea infection and following exogenous jasmonate （JA） or salicylic acid （SA） exposure in the old leaves, but not in the new leaves of the wild-type. In contrast, the accumulation of PR1 proteins was significantly attenuated in old and new leaves of the phyAphyBphyC mutant. These results suggest that phytochromes are required for the induction of PR1 proteins in rice. Basal transcription levels of PRla and PRlb were substantially higher in the wildtype as compared to the phyAphyBphyC mutant, suggesting that phytochromes also are required for basal expression of PR1 genes. Moreover, the transcript levels of genes known to function in SA- or JA-dependent defense pathways were regulated by leaf age and functional phytochromes. Taken together, our findings demonstrate that phytochromes are required in rice for age-related resistance to M. grisea and may indirectly increase PR1 gene expression by regulating SA- and JA-dependent defense pathways.

关键词： Rice phytochrome jasmonate salicylic acid defense.

Metabonomic analysis of hepatitis B virus-induced liver failure:identification of potential diagnostic biomarkers by fuzzy support vector machine

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Journal of Zhejiang university-science B(Biomedicine & {2.) 2008年第6期9卷 474-481页

作者： Yong MAO Xin HUANG Ke YU Hai-bin QU chang-xiao LIU Yi-yu CHENG Pharmaceutical lnformatics institute College of Pharmaceutical Sciences Zhejiang University Hangzhou 310027 China Tianjin Pharmaceutical Research institute National Key Lab Pharmacokinetics and Pharmacodynamics Tianjin 300193 China

Hepatitis B virus （HBV）-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 2. HBV-indueed liver failure patients and 2. healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry （GC-MS） to generate metabolite profiles. The 2. patients were further grouped into two classes according to the severity of liver failure. Twenty-five eommensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor （KNN） and fuzzy support vector machine （FSVM） combined with exhaustive search （ES）, were employed to identify a subset of metabolites （biomarkers） that best predict liver failure. Based on the achieved experimental dataset, 93.62. predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyeerie acid, cis-aeonitie acid and citric acid, are identified as potential diagnostic biomarkers.

关键词： Metabolite profile analysis Potential diagnostic biomarker identification k-nearest neighbor (KNN) Fuzzy supportvector machine (FSVM) Exhaustive search (ES) Gas chromatography-mass spectrometry (GC-MS) Hepatitis B virus (HBV)-induced liver failure

Aerial ammonia exposure induces the perturbation of the interorgan ammonia disposal and branched-chain amino acid catabolism in growing pigs

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Animal Nutrition 2021年第4期7卷 947-958页

作者： shanlong Tang chang Yin Jingjing Xie Jinglin Jiao Liang Chen Lei Liu Sheng Zhang Hongfu Zhang State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China institute of biotechnology Cornell UniversityIthacaNY14853USA

Aerial ammonia exposure leads to tissue damage and metabolic ***,it is unclear how different organs are coordinated to defend against aerial ammonia ***-four pigs were randomly divided into 4 groups,exposed to 0,10,2. or 35 mg/m^(3) ammonia respectively for 2. *** above 2. mg/m^(3) ammonia exposure,decreased aspartate(P=0.016),glutamate(P=0.030)and increased ornithine(P=0.002.were found in the ammonia-removing liver,and after high ammonia(35 mg/m^(3))exposure,glutamine synthetase(GS)expression was increased(P=0.012..An increased glutamate(P=0.004)and decreased glutaminase(GLS)expression(P=0.083)were observed in the lungs after high ammonia *** was also an increasing trend of glutamine in the kidneys after high ammonia exposure(P=0.066).For branched-chain amino acid(BCAA)catabolism,high ammonia exposure increased BCAA content in both the lungs and muscle(P2. mg/m^(3) ammonia exposure increased BCAA only in the lungs(P2.and dehydrogenase complex(BCKDHA/B and DBT)were inhibited to a varying degree in the liver,lungs and muscle after above 2. mg/m^(3) ammonia exposure,especially high ammonia *** expression of BCKDH complex and glutamate-glutamine metabolism-related genes were highly expressed in the liver,followed by the lungs and muscle(P2.expression was highest in the lungs(P=0.002..Altogether,low ammonia exposure sufficed to evoke the urea cycle to detoxify ammonia in the *** process of ammonia removal in the liver and potential ability of the lungs to detoxify ammonia were enhanced with increasing ***,high ammonia exposure impaired the BCAA catabolism and decreased the transcripts of the BCAA catabolism-related enzymes,resulting in high BCAA content in extrahepatic ***,with aerial ammonia increasing,an increased urea cycle and glutamine synthesis were ammonia defensive strategies,and high

关键词： Targeted metabolomics Ammonia exposure Ammonia disposal BCAA catabolism Pig

Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis

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Rice science 2020年第4期27卷 289-301页

作者： YUAN Shuting XU Chunjue YAN Wei chang Zhenyi DENG Xingwang CHEN Zhufeng WU Jianxin TANG Xiaoyan Guangdong Provincial Key Laboratory of biotechnology for Plant Development School of Life SciencesSouth China Normal UniversityGuangzhou 510631China Shenzhen institute of Molecular Crop Design Shenzhen 518107China School of life sciences Southern University of Science and TechnologyShenzhen 518055China

Alternative splicing can generate multiple mRNAs that differ in their untranslated regions or coding sequences,and these differences might affect mRNA stability or result in different protein isoforms with diverse functions and/or *** this study,we isolated a sterile mutant in rice with abnormal meiosis of microspore mother cells and megaspore mother cells that carried a point mutation in OsRAD1 *** of OsRAD1 cDNAs revealed three transcript variants,named as OsRAD1.1,OsRAD1.2.and OsRAD1.3,respectively,which were derived from alternative splicing of the last *** derived from the three transcripts were mostly identical except the difference in the very C-terminal *** three transcripts exhibited similar expression patterns in various tissues,but the expression level of OsRAD1.1 was the *** knockout of OsRAD1.1 led to sterility,while knockout of OsRAD1.2.and OsRAD1.3 together did not change the plant *** of OsRAD1.2.and OsRAD1.3 cDNAs in OsRAD1.1-specific mutant did not complement the plant *** two-hybrid assay showed that OsRAD1.1,but not OsRAD1.2.and OsRAD1.3,interacted with the three other meiosis proteins OsHUS1,OsRAD9 and OsRAD17,suggesting that the C-terminal domain of OsRAD1.1 is critical for the protein function.

关键词： Oryza sativa OsRAD1 alternative splicing meiosis protein interaction fertility sterility

Review:Lipids changes in liver cancer

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Journal of Zhejiang university-science B(Biomedicine & {2.) 2007年第6期8卷 398-409页

作者： JIANG Jing-ting XU Ning ZHANG Xiao-ying WU chang-ping Department of Tumor Biological Treatment. the Third Affiliated Hospital S.zhou University Changzhou 213003. China institute of Laboratory Medicine Lund University S-221 85 Lund. Sweden

Liver is one of the most important organs in energy metabolism. Most plasma apolipoproteins and endogenous lipids and lipoproteins are synthesized in the liver. It depends on the integrity of liver cellular function, which ensures homeostasis of lipid and lipoprotein metabolism. When liver cancer occurs, these processes are impaired and the plasma lipid and lipoprotein patterns may be changed. Liver cancer is the fifth common malignant tumor worldwide, and is closely related to the infections of hepatitis B virus (HBV) and hepatitis C virus (HCV). HBV and HCV infections are quite common in china and other Southeast Asian countries. In addition, liver cancer is often followed by a procession of chronic hepatitis or cirrhosis, so that hepatic function is damaged obviously on these bases, which may significantly influence lipid and lipoprotein metabolism in vivo. In this review we summarize the clinical significance of lipid and lipoprotein metabolism under liver cancer.

关键词： Lipids Lipoprotein Liver cancer

2.猪链球菌磷酸甘油酸激酶基因的克隆表达及酶活性测定

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中国生物工程杂志 2018年第3期38卷 16-23页

作者：郭晓璐龚秀芳陈家锋丁晨曦胡丹潘秀珍王长军中国药科大学生命科学与技术学院南京211198 南京军区疾病预防控制中心南京210002

目的：克隆表达2.猪链球菌中磷酸甘油酸激酶（PGK）并对其酶学特性进行测定。方法：采用PCR方法从05ZYH33基因组中扩增出pgk片段,构建重组表达质粒p ET2.a：pgk,经双酶切及测序验证正确的质粒转化入*** BL2.（DE3）中进行IPTG诱导表达... 详细信息

目的：克隆表达2.猪链球菌中磷酸甘油酸激酶（PGK）并对其酶学特性进行测定。方法：采用PCR方法从05ZYH33基因组中扩增出pgk片段,构建重组表达质粒p ET2.a：pgk,经双酶切及测序验证正确的质粒转化入*** BL2.（DE3）中进行IPTG诱导表达,重组PGK蛋白经SDSPAGE和质谱鉴定并测定其酶学活性。结果：PGK在大肠杆菌中可溶性表达,纯化后得到约43k Da的重组PGK蛋白,其酶促反应最适温度为2.℃,最适pH为7.5,2.猪链球菌PGK的酶活性为75U/ml,PGK相对于3-PGA的Km值为1.744mmol/L,Vmax为0.143mmol/（L·min）,相对于ATP的Km值为2.2.6mmol/L,Vmax为0.318mmol/（L·min）。结论：利用原核表达系统成功地表达了2.猪链球菌中的PGK,并获得了活性较好的重组PGK,酶学检测发现纯化的PGK具有良好的体外活性,为进一步研究该病在2.猪链球菌致病及代谢机制奠定了基础。

关键词： 2.猪链球菌磷酸甘油酸激酶克隆表达酶活性

Coupled aerobic and anoxic biodegradation for quinoline and nitrogen removals

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Frontiers of Environmental science & Engineering 2015年第4期9卷 738-744页

作者： Ning YAN Lu WANG Ling chang Cuiyi ZHANG Yang ZHOU Yongming ZHANG Bruce E. RITTMANN Department of Environmental science and Engineering College of Life and Environmental Science Shanghai Normal University Shanghai 200234 China Swette Center for Environmental biotechnology Biodesign Institute Arizona State University Tempe AZ 85287-5701 USA

Quinoline （C9H7N） commonly occurs in wastewaters from the chemical, pharmaceutical, and dyeing industries. As quinoline is biodegraded, nitrogen is released as ammonium. Total-N removal requires that the ammonium-N be nitrified and then denitrified. The objective of this study was to couple quinoline biodegradation with total-N removal. In a proof-of-concept step, activated sludge was sequenced from aerobic to anoxic stages. The ammonium nitrogen released from quinoline biodegradation in the aerobic stage was nitrified to nitrate in parallel. Anoxic biodegradation of the aerobic effluent then brought about nitrogen and COD removals through denitrification. Then, simultaneous quinoline biodegradation and total-N removal were demonstrated in a novel airlift internal loop biofilm reactor （AILBR） having aerobic and anoxic zones. Experimental results showed that the AILBR could achieve complete removal of quinoline, 91% COD removal, and 85% total-N removal when glucose added as a supplemental electron donor once nitrate was formed.

关键词： Quinoline biofilm reactor biodegradation,denitrification

Reliable protocols for whole-mount fluorescent in situ hybridization （FISH） in the pea aphid Acyrthosiphon pisum： A comprehensive survey and analysis

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Insect science 2014年第3期21卷 265-277页

作者： Chen-yo Chung Charles E. Cook Gee-way Lin Ting-Yu Huang Chun-che chang Laboratory for Genetics and Development Department ofEntomologyllnstitute of Biotechnology College ofBioresources andAgriculture National Taiwan University Taipei Taiwan European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI) Wellcome Trust Genome Campus Hinxton Cambridge UK Research Center for Developmental Biology and Regenerative Medicine National Taiwan university Taipei Taiwan institute of Biochemical sciences College of Life Science National Taiwan University Taipei Taiwan Genome and Systems Biology Degree Program National Taiwan University Taipei Taiwan China

RNA in situ hybridization （ISH）, including chromogenic ISH （CISH） and fluorescent ISH （FISH）, has become a powerful tool for revealing the spatial distribution ofgene transcripts in model organisms. Previously, we developed a robust protocol for wholemount RNA CISH in the pea aphid Acyrthosiphon pisum, an emerging insect genomicmodel. In order to improve the resolving capacity of gene detection, we comprehensively surveyed current protocols of whole-mount RNA-FISH and developed protocols that allow,using confocal microscopy, clearer visualization of target messenger RNAs （mRNAs） - including those subcellularly localized and those with spatially overlapping expression. Wefind that Fast dye-based substrate fluorescence （SF）, tyramide signal amplification （TSA）, and TSA Plus all enable identifying gene expression thanks to multiplex amplificationof fluorescent signals. By contrast, methods of direct fluorescence （DF） do not allow visualizing signals. Detection of a single gene target was achieved with SF and TSA Plusfor most mRNAs, whereas TSA only allowed visualization of abundant transcripts such as Apvasl andAppiwi2.in the germ cells. For detection of multiple gene targets using doubleFISH, we recommend：（i） TSA/TSA, rather than TSA Plus/TSA Plus for colocalized mRNAs abundantly expressed in germ ceils, as proteinase K treatment can be omitted;and （ii） SF/TSA Plus for other gene targets such as Apenl and Apen2.as inactivation of enzyme conjugates is not required. SF/SF is not ideal for double FISH experiments due tosignal blurring. Based on these new conditions for RNA-FISH, we have obtained a better understanding of germline specification and embryonic segmentation in the pea *** anticipate that the RNA-FISH protocols for the pea aphid may also be used for other aphids and possibly other insect species, thus expanding the range of species from whichuseful insights into development and evolution may be obtained.

关键词： aphid fluorescence gene expression in situ hybridization model organism probe detection