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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

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Chinese science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2.3,4, WANG Kemin1,2.3,4, XIAO Zhiqiang5,6, WANG Wei1,3,4, TAN Weihong1,2.3,4, SUN Yi5,6, TANG Hongxing1,3,4 & YANG Xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan university, chang{2. 4.0082, china 2. life science and biotechnology institute, hunan university, chang- sha 4.0082, china 3. Chemistry and Chemical Engineer College, hunan university, chang{2. 4.0082, china 4. hunan Engineering Research Center for Bio-Nanotechnology, hunan university, chang{2. 4.0082, china 5. Medical Experiment Research Center, Xiangya Hospital, Central South university, chang{2. 410008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, chang{2. 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China life science and {2. institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China hunan Engineering Research Center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research Center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

Practical Synthesis of Valbenazine via 1,3-Dipolar Cycloaddition

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Chinese Journal of Chemistry 2024年第8期42卷 841-845页

作者： Yalan Peng Zuming Lin Lili Zhu Shiqing Han sha-Hua Huang Ran Hong College of biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjingJiangsu211816 China School of Environmental and Chemical Engineering Shanghai Institute of TechnologyShanghai201418 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032 China Innovation Research institute of Traditional Chinese Medicine(IRI) Shanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai201203 China

Comprehensive Summary,Valbenazine(Ingrezza),a potent and highly selective inhibitor of vesicular monoamine transporter type 2.VMAT2.through the active metabolite hydrotetrabenazine(HTBZ),has been approved for the treatment of tardive dyskinesia and,very recently,for chorea,which is associated with Huntington's *** numerous synthetic efforts dedicated to the synthesis of HTBZ,the industrial preparation of valbenazine uses dihydroisoquinoline as a starting material and the chiral resolution of racemic HTBZ derived from ketone ***,we present a practical synthesis of HTBZ and valbenazine featuring a highly stereoselective 1,3-dipolar cycloaddition and enzymatic kinetic *** cascade process includes cycloaddition,N—O bond cleavage,and lactamization,which proved to be operationally *** allure of the enzymatic resolution developed in this work offers a rapid access toward affording tetrahydroisoquinoline(THIQ)-fused piperidine in the production of medically significant compounds,such as yohimbine and reserpine.

关键词： Cycloaddition Chemoenzymatic Kinetic resolution Prodrugs VMAT2.inhibitor

Effect of Waterlogged and Aerobic Incubation on Enzyme Activities in Paddy Soil

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Pedosphere 2006年第4期16卷 532-539页

作者： WANG Xiao-chang LU Qin Department of Tea science College of Agriculture and Biotechnology Zhejiang University Hangzhou 31002g （China） institute of Soil science Chinese Academy of Sciences Nanjing 210008 （China）

An incubation experiment with soil water content treatments of 0.15 （W1）, 0.2. （W2., and 0.40 （W3） g g^-1 soil was carried out for two months to investigate the activities of important enzymes involved in C, N, P, and S cycling in a paddy soil from the Taihu Lake region, china, under waterlogged and aerobic conditions. Compared with air-dried soil, waterlogging resulted in a significant decrease （P ≤ 0.05） of fluorescein diacetate （FDA） and /3-D-glucosidase activities, and this effect was enhanced with increasing waterlogging time. Waterlogging also significantly inhibited （P ≤ 0.05） arylsulfatase as well as alkaline and acid phosphatase activities, but did not decrease the activities with the increase in waterlogging time. Short-term waterlogging did not affect urease activity, but prolonged waterlogging decreased it markedly. In contrast, the aerobic incubation （W1 and W2.treatments） significantly increased （P ≤ 0.05） FDA, alkaline phosphatase, and /3-D-glucosidase activities. With aerobic treatments the activities of FDA and alkaline phosphatase increased with incubation time, whereas /3-D-glucosidase activity decreased. A significant difference （P ≤ 0.05） was usually observed between the W1 and W2.treatments for the activities of FDA as well as alkaline and acid phosphatase; however,/3-D-glucosidase and urease were usually not significant （P ≤ 0.05）. No activity differences were observed between waterlogging and aerobic incubation for arylsulfatase and urease.

关键词： aerobic incubation enzyme activity paddy soil waterlogging

Ionic Liquid-Enhanced Assembly of Nanomaterials for Highly Stable Flexible Transparent Electrodes

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Nano-Micro Letters 2024年第7期16卷 441-455页

作者： Jianmin Yang Li chang Xiqi Zhang Ziquan Cao Lei Jiang Key Laboratory of Bio-Inspired Materials and Interfacial science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China university of Chinese Academy of sciences Beijing 100049People’s Republic of China College of Chemistry and Chemical Engineering Chongqing University of TechnologyChongqing 400054People’s Republic of China Binzhou institute of Technology Binzhou 256600People’s Republic of China Nanomics biotechnology Co. Ltd.HangzhouPeople’s Republic of China

The controlled assembly of nanomaterials has demon-strated significant potential in advancing technological ***,achieving highly efficient and low-loss assembly technique for nanomate-rials,enabling the creation of hierarchical structures with distinctive func-tionalities,remains a formidable ***,we present a method for nanomaterial assembly enhanced by ionic liquids,which enables the fabrication of highly stable,flexible,and transparent electrodes featuring an organized layered *** utilization of hydrophobic and non-volatile ionic liquids facilitates the production of stable interfaces with water,effectively preventing the sedimentation of 1D/2. nanomaterials assembled at the ***,the interfacially assembled nanomaterial monolayer exhibits an alternate self-climbing behavior,enabling layer-by-layer transfer and the formation of a well-ordered MXene-wrapped silver nanowire network *** resulting composite film not only demonstrates exceptional photoelectric performance with a sheet resistance of 9.4Ωsq^(-1) and 93%transmittance,but also showcases remarkable environmental stability and mechanical *** noteworthy is its application in transparent electromagnetic interference shielding materials and triboelectric nanogenerator *** research introduces an innovative approach to manufacture and tailor functional devices based on ordered nanomaterials.

关键词： Ionic liquids Assembly Silver nanowires MXene nanosheets Flexible transparent electrodes

Establishment of a transformation system in close relatives of wheat under the assistance of TaWOX5

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Journal of Integrative Agriculture 2024年第6期23卷 1839-1849页

作者： Yanan chang Junxian Liu chang Liu Huiyun Liu Huali Tang Yuliang Qiu Zhi{2.n Lin Ke Wang Yueming Yan Xingguo Ye institute of Crop sciences Chinese Academy of Agricultural SciencesBeijing 100081China Beijing Key Laboratory of Plant Gene Resources and biotechnology for Carbon Reduction and Environment Improvement/College of life {2. Capital Normal UniversityBeijing 100048China College of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome Engineering/institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

Species closely related to wheat are important genetic resources for agricultural production,functional genomics studies and wheat *** this study,a wheat gene related to regeneration,TaWOX5,was applied to establish the Agrobacterium-mediated transformation systems of Triticum monococcum,hexaploid triticale,and rye(Secale cereale L.)using their immature *** plants were efficiently *** the transformation process,the Agrobacterium infection efficiency was assessed by histochemical staining forβ-glucuronidase(GUS).Finally,the transgenic nature of regenerated plants was verified by polymerase chain reaction(PCR)-based genotyping for the presence of the GUS and bialaphos resistance(bar)genes,histochemical staining for GUS protein,and the QuickStix strip assay for bar *** transformation efficiency of *** genotype PI42.182.was 94.4%;the efficiencies of four hexaploid triticale genotypes Lin456,ZS32.7,ZS12.7,and ZS32.4 were 52.1,41.2.19.4,and 16.0%,respectively;and the transformation efficiency of rye cultivar Lanzhou Heimai was 7.8%.Fluorescence in situ hybridization(FISH)and genomic in situ hybridization(GISH)analyses indicated that the GUS transgenes were integrated into the distal or near centromere(proximal)regions of the chromosomes in transgenic *** and hexaploid triticale *** the transgenic hexaploid triticale plants,the foreign DNA fragment was randomly integrated into the AABB and RR ***,the transgene was almost stably inherited in the next generation by Mendel’s *** findings in this study will promote the genetic improvement of the three plant species for grain or forage production and the improvement of cereal species including wheat for functional genomics studies.

关键词： Triticum monococcum hexaploid triticale rye TaWOX5 Agrobacterium transformation efficiency

Nonuniform stretch processing for the range profile of a target with micro-motion

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Progress in Natural science:Materials International 2006年第11期16卷 1205-1213页

作者： CHEN Hangyong**, JIANG Weidong, LIU Yongxiang, LI Xiang and GUO Guirong(institute of Space Electronics Information Technology, National university of Defense Technology, {2. sha 410073, china) institute of Space Electronics Information Technology National University of Defense Technology Chang Sha 410073 China

Complex motions such as micro-motions induce complex range-Doppler coupling effect in radar imaging. Hence, it is a challenge to reconstruct a clear range profile of a target with micro-motion. Stretch processing with fine motion compensation cannot perform well while it ties to eliminate the distortion of range profile caused by Doppler effect, because it is difficult to accurately estimate each scattering center velocity during coherent processing interval. Based on nonuniform stretch processing (NSP), a new technology is proposed to decouple range, time and Doppler and then achieve range profile of target with micro-motion. The new technology uses a new synthetic wide-band waveform and has an innovative mathematic interpretation. Statistical properties of the new approach are primarily examined. Typical models of target with micro-motion for high resolution radar are presented. Monte Carlo simulations demonstrate the performance of the new technology and the simulation results confirm our expectations.

关键词： synthetic range profile micro-motion stretch processing Doppler effect.

Zinc glycine chelate ameliorates DSS‑induced intestinal barrier dysfunction via attenuating TLR4/NF‑κB pathway in meat ducks

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Journal of Animal science and biotechnology 2024年第2期15卷 765-778页

作者： Yaqi chang Ke Wang Guangmang Liu Hua Zhao Xiaoling Chen Jingyi Cai Gang Jia institute of Animal Nutrition Key Laboratory for Animal Disease‑Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural UniversityChengdu 611130SichuanChina

Background Zinc glycine chelate(Zn-Gly)has anti-inflammation and growth-promoting properties;however,the mechanism of Zn-Gly contribution to gut barrier function in Cherry Valley ducks during intestinal inflammation is ***-hundred 1-day-old ducks were divided into 5 groups(6 replicates and 10 ducks per replicate)in a completely randomized design:the control and dextran sulfate sodium(DSS)groups were fed a corn-soybean meal basal diet,and experimental groups received supplements of 70,12. or 170 mg/kg Zn in form of *** DSS and treatment groups were given 2.mL of 0.45 g/mL DSS daily during d 15–2.,and the control group received normal *** experiment lasted 2. *** Compared with DSS group,70,12. and 170 mg/kg Zn significantly increased body weight(BW),villus height and the ratio of villus to crypt,and significantly decreased the crypt depth of jejunum at 2. *** number of goblet cells in jejunal villi in the Zn-Gly group was significantly increased by periodic acid-Schiff *** with control,the content of intestinal permeability marker D-lactic acid(D-LA)and fluxes of fluorescein isothiocyanate(FITC-D)in plasma of DSS group significantly increased,and 170 mg/kg Zn supplementation significantly decreased the D-LA content and FITC-D *** with control,contents of plasma,jejunum endotoxin and jejunum pro-inflammatory factors IL-1β,IL-6 and TNF-αwere significantly increased in DSS group,and were significantly decreased by 170 mg/kg Zn *** Zn significantly increased the contents of anti-inflammatory factors IL-10,IL-2. and sIgA and IgG in ***-time PCR and Western blot results showed that 170 mg/kg Zn supplementation significantly increased mRNA expression levels of CLDN-1 and expression of OCLN protein in jejunum,and decreased gene and protein expression of CLDN-2.compared with DSS *** 12. mg/kg Zn significantly promoted the expressions of IL-2. and *** Zn-Gly supplementation sign

关键词： Cherry Valley ducks Growth performance Gut inflammation Intestinal barrier Zn-Gly

Population genomic data reveal low genetic diversity,divergence and local adaptation among threatened Reeves's Pheasant(Syrmaticus reevesii)

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Avian Research 2024年第1期15卷 1-11页

作者： Qi Lu Pengcheng Wang Jiang chang De Chen Shenghan Gao Jacob Hoglund Zhengwang Zhang Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijing100875China Jiangsu Key Laboratory for Biodiversity and biotechnology College of Life SciencesNanjing Normal UniversityNanjing210023China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing100012China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Department of Ecology and Genetics Animal EcologyUppsala UniversityUppsalaSweden

Population genomic data could provide valuable information for conservation efforts;however,limited studies have been conducted to investigate the genetic status of threatened ***’s Pheasant(Syrmaticus reevesii)is facing population decline,attributed to increases in habitat *** is a knowledge gap in understanding the genomic status and genetic basis underlying the local adaptation of this threatened ***,we used population genomic data to assess population structure,genetic diversity,inbreeding patterns,and genetic ***,we identified candidate genes linked with adaptation across the current distribution of Reeves’s *** present study assembled the first de novo genome sequence of Reeves’s Pheasant and annotated 19,458 *** also sequenced 30 individuals from three populations(Dabie Mountain,Shennongjia,Qinling Mountain)and found that there was clear population structure among those *** comparing with other threatened species,we found that Reeves’s Pheasants have low genetic *** of homozygosity suggest that the Shennongjia population has experienced serious *** demographic history results indicated that three populations experienced several declines during the glacial *** adaptative analysis among the populations identified 2.1 candidate genes under directional *** are involved in a large variety of processes,including the immune response and *** results suggest that the three populations should be considered as three different conservation *** current study provides genetic evidence for conserving the threatened Reeves’s Pheasant and provides genomic resources for global biodiversity management.

关键词： Conservation genetics Local adaptation Pheasant Whole-genome sequencing

Climate change drives flooding risk increases in the Yellow River Basin

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Geography and Sustainability 2024年第2期5卷 193-199页

作者： Hengxing Lan Zheng Zhao Langping Li Junhua Li Bojie Fu Naiman Tian Ruixun Lai sha Zhou Yanbo Zhu Fanyu Zhang Jianbing Peng John J.Clague State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China School of Geological Engineering and Geomatics Chang’an UniversityXi’an 710064China university of Chinese Academy of sciences Beijing 10049China Yellow River institute of Hydraulic Research Yellow River Conservancy CommissionZhengzhou 450003China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China State Key Laboratory of Earth Surface Processes and Resources Ecology Beijing Normal UniversityBeijing 100875China Key Laboratory of Mechanics on Disaster and Environment in Western china The Ministry of Education of ChinaSchool of Civil Engineering and MechanicsLanzhou UniversityLanzhou 730000China Department of Earth sciences Simon Fraser UniversityBurnabyCanada

The Yellow River Basin(YRB)has experienced severe floods and continuous riverbed elevation throughout *** climate change has been suggested to be driving a worldwide increase in flooding ***,owing to insufficient evidence,the quantitative correlation between flooding and climate change remains *** present a long time series of maximum flood discharge in the YRB dating back to 1843 compiled from historical documents and instrument *** in yearly maximum flood discharge show distinct periods:a dramatic decreasing period from 1843 to 1950,and an oscillating gentle decreasing from 1950 to 2.2.,with the latter period also showing increasing more extreme floods.A Mann-Kendall test analysis suggests that the latter period can be further split into two distinct sub-periods:an oscillating gentle decreasing period from 1950 to 2.00,and a clear recent increasing period from 2.00 to *** further predict that climate change will cause an ongoing remarkable increase in future flooding risk and an∼44.4 billion US dollars loss of floods in the YRB in 2.00.

关键词： Flooding risk Risk management Climate change Flood discharge Extreme precipitation