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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, institute of Hydrobiology, chinese {2. of Sciences, Wuhan 430072. china 2. Graduate School of chinese {2. of Sciences, beijing 100039, china) [a]State key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]Graduate School of chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

A new principle of synthetic cascade utilization of chemical energy and physical energy

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Science china(Technological Sciences) 2005年第2期48卷 163-179页

作者： JIN Hongguang1, HONG Hui1,2. WANG Baoqun1,2. HAN Wei1,2.& LIN Rumou1,2.1. institute of Engineering Thermophysics, {2. academy of Sciences, beijing 100080, china 2. Graduate School of the {2. academy of Sciences, beijing 100039, china 1. institute of Engineering Thermophysics Chinese Academy of Sciences 100080 Beijing China 2. Graduate School of the chinese academy of Sciences 100039 Beijing China

We propose a new principle of the cascade utilization of both chemical energy and physical energy in energy systems with the integration of chemical processes and thermal cycles. Particularly, a general equation of energy levels of substance, Gibbs free energy of chemical reaction and physical energy is explicitly founded. On the basis of this equation, a chemical-looping combustion and an indirect combustion are investigated. Furthermore, a mechanism of energy release, with the combination of decreasing the energy level of Gibbs free energy and upgrading the energy level of low or middle- temperature thermal energy, is clarified. The promising results obtained here establish a theoretical basis for the further investigation of multi-function systems in which energy and the environment are compatible, and create a new approach to improve the performance of traditional thermal cycles.

关键词： chemical energy, cascade utilization, exergy, energy conversion.

Linear relationship between the interdecadal and interannual variabilities of North china rainfall in rainy season

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chinese Science Bulletin 2003年第10期48卷 1040-1044页

作者： LU Riyu institute of atmospheric physics, chinese {2. of Sciences, beijing 100080, china institute of atmospheric {2. Chinese Academy of Sciences Beijing 100080 China

Interdecadal and interannual timescales are dominant in the North china rainfall in rainy season (July and August). On the interdecadal timescale, the North china rainfall exhibited an abrupt decrease at the end of 1970s. In this study, we examined the effect of this abrupt rainfall decrease on the association between rainfall and circulation on the interannual timescale, and found that the interdecadal variation does not change the physical mechanism responsible for the interannual variation of North china rainfall. There is a linear relationship between the interdecadal and interannual variabilities of North china rainfall in rainy season.

关键词：线性关系中国北部降雨量大气环流

Approaches to improving critical current density of YBCO superconducting thick films deposited by electrophoresis

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Science china(physics,Mechanics & Astronomy) 2004年第2期47卷 165-172页

作者： ZHU Yabin1,2. ZHOU Yueliang2. LIU Zhen2. WANG Shufang2. CHEN Zhenghao2. L?Huibin2. YANG Guozhen2. XIAO Ling3, REN Hongtao3, JIAO Yulei3 & ZHENG Minghui3 1. Department of physics, College of Science, beijing Jiaotong University, beijing 100044, china 2. Laboratory of Optical physics, institute of physics and Center for Condensed Matter physics, chinese academy of Sciences, beijing 100080, china 3. General Research institute for Nonferrous Metals, beijing 100088, china Correspondence should be addressed to Zhu Yabin (email: ybzhu@***) 1. Department of physics College of Science Beijing Jiaotong University 100044 Beijing China 2. Laboratory of Optical physics institute of physics and Center for Condensed Matter physics Chinese Academy of Sciences 100080 Beijing China 3. General Research institute for Nonferrous Metals 100088 Beijing China

Fabrication of YBCO superconducting thick films by electrophoresis has been widely reported. However, the value of critical current density (J c) is far from what the practical application requires. The superconducting thick films are obtained using top-seeded melt growth method with different proportional Y2.aCuO5 (Y2.1) addition. High-pressure oxygen has been used in the annealing process to improve the uniformity of the oxygen content in the superconducting thick films. When 40mol% Y2.1 powder is added to the YBa2.u3O7–5 (Y12.) powder,J c for the superconducting thick film by approaches described above reaches 7.008×103 A/cm2.(77 K, 0 T), which is greater than the value ever reported.

关键词： YBCO thick films, electrophoretic deposition, melt-textured.

Simulating the impacts of climate variation and land-cover changes on basin hydrology: A case study of the Suomo basin

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Science china Earth Sciences 2005年第9期48卷 1501-1509页

作者： CHEN Junfeng1,2. LI Xiubin2.& ZHANG Ming2.1. institute of Remote Sensing & GIS, Peking University, beijing 100871,{2. 2. institute of Geographical Sciences and Natural Resources Research, chinese academy of Sciences, beijing 100101,{2. 1. institute of Remote Sensing & GIS Peking University 100871 Beijing China 2. institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences 100101 Beijing Chin

Impacts of land cover changes on watershed hydrology have been a long-term academic concern with acute dispute. But little attention has been paid to such effects on mesoscale river basins, where the society has a closer link to river hydrology. The present study focuses on a mesoscale river basin, the Suomo Basin that is located on the upper reaches of the Yangtze River. Land covers in the basin in the years 1970, 1986 and 1999 were mapped. A lumped hydrologic model, CHARM, and a distributed hydrologic model, SWAT, were used to model the impacts of both land-cover change and climate variation on river runoff during the past four decades. The results show that the contribution of climate variation to the change of runoff regime makes up 60%―80%, while that of land cover changes only 2.%.

关键词： climate variation, land-cover changes, Suomo Basin, CHARM model, SWAT model.

High-temperature phases of pyrophyllite and their evolutionary characteristics

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chinese Science Bulletin 1997年第2期42卷 140-144页

作者： WANG Ling and ZHANG Zhenyu1. Changsha institute of Geotectonics, chinese {2. of Sciences, Changsha 410013, china 2. institute of Geology, chinese {2. of Sciences, beijing 10002., china Changsha institute of Ceotectonics Chinese Academy of Sciences Changsha China institute of Geology Chinese Academy of Sciences Beijing China

WITH the increase of heating temperature, phase of pyrophyllite will undergo a series of transformations. Former scholars have done some work in this respect; the temperature spans,however, were normally not more than 1150℃ and no relatively comprehensive results have been reported. Thus, the features of the phases from indoor temperature to melting point (1710℃±) for pyrophyllite and the entire evolutionary process of the phases are uncertain.

关键词： pyrophyllite high-temperature phase phase tranformation pyrophyllite anhydrite mullite cristobalite.

Human remains and the environment of Early Pleistocene in the Nihewan Basin

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Science china Earth Sciences 2004年第5期47卷 437-444页

作者： CAI Baoquan1 & LI Qiang2.1. Department of History, Xiamen University, Fujian 361005, china 2. institute of Vertebrate Palaeontology and Palaeoanthropology, {2. academy of Sciences, beijing 100044, china Xiamen Univ Dept Hist Fujian 361005 Peoples R China chinese Acad Sci Inst Vertebrate Paleontol & Paleoanthropol Beijing 100044 Peoples R China

A new Early Pleistocene Paleolithic site was found in July 2.01 in the Nihewan Ba-sin, Hebei Province. Totally 500 mammal specimens assigned to 2. species and 5 lithic artifacts were collected. The coexistence of Allophaiomys deucalion, Borsodia chinensis and Yangia tingi provides important evidence of chronology. On the basis of the comparison of mammalian fauna, the date of Paleolithic artifacts is probably earlier than 1.8 MaBP, possibly 2.0 MaBP. This is the earliest evidence of hominid activity found so far in North china. The hominid at that time in the Nihewan Basin lived in an environment of arid grasslands with scattered trees of temperate zone. This discovery is significant to the study of human origin and cultural development.

关键词： human remains, environment, Early Pleistocene, Nihewan.

Simulation of the future change of East Asian monsoon climate using the IPCC SRES A2.and B2.scenarios

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chinese Science Bulletin 2003年第10期48卷 1024-1030页

作者： BUEH Cholaw LASG, institute of atmospheric physics, chinese {2. of Sciences, beijing 10002., china LASG Institute of Atmospheric Physics Chinese Academy of Sciences China

In this paper, we applied the newest emission scenarios of the sulfur and greenhouse gases, i.e. Intergovernmental Panel on Climate Change (IPCC) Special Report on Emission Scenarios (SRES) A2.and B2.scenarios, to investigating the change of the East Asian climate in the last three decades of the 2.st century with an atmosphere-ocean coupled general circulation model. The global warming enlarges the land-sea thermal contrast and, hence, enhances (reduces) the East Asian summer (winter) monsoon circulation. The precipitation from the Yangtze and Huaihe river valley to North china increases significantly. In particular, the strong rainfall increase over North china implies that the East Asian rainy area would expand northward. In addition, from the southeastern coastal area to North china, the rainfall would increase significantly in September, implying that the rainy period of the East Asian monsoon would be prolonged about one month. In July, August and September, the interannual variability of the precipitation enhances evidently over North china, meaning a risk of flooding in the future.

关键词：模拟亚洲东方季风气侯变化大气循环模型

Effect of concentration on surfactant micelle shapes——A molecular dynamics study

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Science china Chemistry 2005年第5期48卷 470-475页

作者： GAO Jian1,2. GE Wei1 & LI Jinghai1 1. Multiphase Reaction Laboratory, institute of Process Engineering, chinese {2. of Sciences, beijing 100080, china 2. Graduate School of The chinese {2. of Sciences, beijing 100085, china 1. Multiphase Reaction Laboratory Institute of Process Engineering Chinese Academy of Sciences 100080 Beijing China 2. Graduate School of The chinese academy of Sciences 100085 Beijing China

Many aspects of the behavior of surfactants have not been well understood due to the coupling of many different mechanisms. Computer simulation is, therefore, attractive in the sense that it can explore the effect of different mechanisms separately. In this paper, the shapes, structures and sizes of sodium dodecylbenzenesulfonate (SDBS) micelles under different con-centrations in an oil/water mixture were studied via molecular dynamics (MD) simulations using a simplified atomistic model which basically maintains the hydrophile and lipophile properties of the surfactant molecules. Above the critical micellar concentration (cmc), surfactant molecules ag-gregate spontaneously to form a wide variety of assemblies, from spherical to rodlike, wormlike and bilayer micelles. Changes in their ratios of the principle moments of inertia (g1/g3, g2.g3) in-dicated the transition of micelle shapes at different concentrations. The aggregation number of micelle is found to have a power-law dependence on surfactant concentration.

关键词： surfactant, MD simulation, sodium dodecylbenzenesulfonate, micelle shape.