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Protein phosphorylation is involved in the water stress-induced ABA accumulation in the roots of Malus hupehensis Rehd

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Chinese Science Bulletin 2001年第10期46卷 855-858页

作者： ZHANG Dapeng, YANG Hongqiang, JIA Wensuo & HUANG Conglin1. laboratory of Molecular Developmental Biology of Fruit Trees, china Agricultural {2., beijing 100094, china 2. The key laboratory of plant physiology and biochemistry of Agricultural ministry, china Agricultural {2., beijing 100094, china laboratory of Molecular Developmental Biology of Fruit Trees China Agricultural University Beijing China The key laboratory of {2. physiology and biochemistry of Agricultural ministry China Agricultural University Beijing China

Water stress-induced ABA accumulation plays a key role in the root to shoot communication and/or the cell to cell signaling under the soil stresses. The signaling of the water stress itself that leads to the accumulation, however, is less known. In this study, we subjected the roots of Malus hupehensis seedlings to water stress treatment and investigated the ABA accumulation in relation to protein phos-phorylation. Our results showed that ABA accumulation could be substantially triggered in 40 min and reached 4 folds in 100 min after treatment with 30% PEG 6000 (polyethylene glycol). The water stress treatment also led to a substantial enhancement of total kinase activity, assessed with histone-111 as substrate, in 15 min and a maximum enhancement in 30 min before it declined to initial level. The Ca2.-dependent kinase activity showed a similar, if not more sensitive, trend. When the roots were fed with labeled 32.-ATP, water stress enhanced the labeling of proteins, which showed a maximum labeling at

关键词： ABA (abscisic acid) water stress protein kinases Malus hupehensis Rehd.

Ammonium improves iron nutrition by decreasing leaf apoplastic pH of sunflower plants(Helianthus annuus ***)

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Chinese Science Bulletin 2003年第20期48卷 2215-2220页

作者： ZOU Chunqin & ZHANG Fusuo key laboratory of {2.-Soil Interactions, ministry of Education key laboratory of {2. Nutrition, ministry of agriculture of china Department of {2. Nutrition, china Agricultural {12., beijing 100094, china Correspondence should be addressed to Zhang Fusuo (e-mail: zhangfs@ ***) key laboratory of {2.-Soil Interaction Ministry of Education Key Laboratory of Plant Nutrition Ministry of Agriculture of China Department of Plant Nutrition China Agricultural University Beijing China

The effect of nitrogen form on pH and concentration of soluble iron (Fe) in leaf apoplast was investigated in hydrophonically grown sunflower plants (Helianthus annuus L. cv. Frankasol), and the mechanism underlying the improved Fe nutrition by ammonium (NH4) supply was also elucidated. Ammonium supply ameliorated Fe nutrition of plants grown without Fe through decreasing apoplastic pH and increasing soluble Fe concentration in apoplastic fluid of young leaves. The soluble Fe concentration in apoplastic fluid and cell sap of young leaves, and xylem exudates of NH4 fed-plants was higher than that of nitrate (NO3) fed-plants, and no typical Fe-deficiency chlorosis in young leaves was observed in NH4 fed plant without Fe supply. The apoplastic pH was 6.15 and 5.94 in young leaves of Fe-deficient plants fed respectively with NO3 and NH4, while in Fe-sufficient plants, the apoplastic pH was 6.43 with NO3, and 5.50 with NH4 supply. In primary leaves, the apoplastic pH was around 6.2. irrespective of nitrogen form and Fe supply. The pH of xylem exudate was 5.72.in Fe-deficient plants fed with NO3 and 5.49 with NH4. Iron nutrition increased the pH of xylem exudate by 0.2. and 0.16 unit under NO3 and NH4 supply respectively.

关键词：向日葵铁营养学非原质体 pH值氮铵赋存形态植物生理学

Cloning and characterization of an up-regulated GA 2.-oxidase gene in hybrid maize

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Progress in Natural Science:Materials International 2009年第2期19卷 161-166页

作者： Jinkun Du,Yingyin Yao,Zhongfu Ni,Qixin Sun Department of plant Genetics & Breeding and State key {2. for Agrobiotechnology,key {2. of Crop Genomics and Genetic Improvement,ministry of agriculture/beijing key {2. of Crop Genetic Improvement,china Agricultural university,beijing 100094,china [a]Department of plant Genetics & Breeding and State key {2. for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Previous studies revealed that GA content and metabolism are positively correlated with a faster shoot growth rate of hybrid,and recently,genes participating in both GA biosynthesis and GA response pathways were also found to be differentially expressed between wheat hybrid and its parental inbreds. In this study,an up-regulated GA 2.-oxidase gene in a maize hybrid,designated ZmGA2.,was cloned. ZmGA2. contains an open reading frame (ORF) encoding 391 amino acid residues. BLASTX searches in GenBank revealed that the ZmGA2. is homologous to the sequences of GA2.ox proteins from different species,and analysis also indicated that ZmGA2. had typical features of GA 2.-oxidase proteins,including a "LPWKET" sequence. Semi-quantitative RT-PCR analysis showed that ZmGA2. was expressed in different tissues and/or organs. The expression level of ZmGA2. in the hybrid was higher than that in two parents (in roots,leaves,stems and embryo,and ears). The abundance of ZmGA2. transcript was equal to that of the highly expressed parents,which provided molecular evidence for the observed GA content heterosis in maize hybrids.

关键词： GA 2.-oxidase Gene expression Maize Heterosis

Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.)

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Progress in Natural Science:Materials International 2008年第6期18卷 697-705页

作者： Hualing Wu,Zhongfu Ni,Yingyin Yao,Ganggang Guo,Qixin Sun* Department of plant Genetics&Breeding and State key {2. for Agrobiotechnology,key {2. of Crop Heterosis and Utilization,ministry of Education,key {2. of Crop Genomics and Genetic Improvement,ministry of agriculture/beijing key {2. of Crop Genetic Improvement,china Agricultural university,beijing 100094,china Department of plant Genetics & Breeding and State key {2. for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization Ministry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

WRKY proteins are involved in various physiological processes,including biotic and abiotic stress responses,hormone responses and ***,no systematic identification,expression and function analysis of WRKY genes in wheat were *** this study,we isolated 15 wheat cDNAs with complete open reading frame(ORF)encoding putative WRKY proteins using in silico *** analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY *** analysis revealed that most genes expressed drastically in leaf,except TaWRKY10 which expressed in crown *** genes were strongly up-reg-ulated with the senescence of *** genes were responsive to low temperature,high temperature,NaCl or PEG ***-over,dierential expression patterns were also observed between wheat hybrid and its parents,and some genes were more responsive to PEG treatment in the *** results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic *** the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids.

关键词： Wheat WRKY transcription factor Gene cloning Gene expression Heterosis

Isolation and expression of an aquaporin-like gene VfPIP1 in Vicia faba

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Progress in Natural Science:Materials International 2005年第6期15卷 496-501页

作者： CUI Xianghuan, HAO Fushun, CHEN Hui, CAI Jinghui, CHEN Jia and WANG Xuechen*(State key laboratory for {2. physiology and biochemistry, College of Biological Science, china Agricultural university, beijing 100094, china) State key laboratory for {2. physiology and biochemistry College of Biological Science China Agricultural University Beijing 100094 China

To explore the effects of aquaporins on stomatal movement, we isolated a full length cDNA of aquaporin-like gene VfPIP1 (Vicia faba plasma membrane intrinsic protein gene, GenBank accession number: AY667436), which encodes for a 2.0-amino-acid polypeptide, from Vicia faba leaf epidermis by 5′/3′ RACE (Rapid Amplification of cDNA Ends). The analyses of VfPIP1 transmembrane regions and amino acid sequence show that VfPIP1 owns six membrane-spanning domains and the special plasma membrane signature sequences GGGANXXXXGY and TGI/TNPARSL/FGAAI/VI/VF/YN, and it should be a member of PIP1 subfamily. The results of in situ hybridization and Northern blot indicate that VfPIP1 is strongly expressed in guard cells and induced by ABA. Hereby, VfPIP1 may be involved in the water-transmembrane movement of guard cells.

关键词： ABA aquaporin gene cloning Vicia faba guard cell.

Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)

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Cell Research 2004年第5期14卷 407-414页

作者： Ai Xiao LIU*, Shao Bin ZHANG*, Xiao Jing XU, Dong Tao REN, Guo Qin LIU** State key laboratory of {2. physiology and biochemistry, College of Biological Science, china Agricultural university, beijing 100094, china. State key laboratory of {2. physiology and biochemistry College of Biological Science China Agricultural University Beijing 100094 China.

A pea actin isoform PEAc1 with green fluorescent protein (GFP) fusion to its C-terminus and His-tag to its N- terminus, was expressed in prokaryotic cells in soluble form, and highly purified with Ni-Chelating SepharoseTM Fast Flow column. The purified fusion protein (PEAc1-GFP) efficiently inhibited DNase I activities before polymerization, and activated the myosin Mg-ATPase activities after polymerization. The PEAc1-GFP also polymerized into green fluorescent filamentous structures with a critical concentration of 0.75 μM. These filamentous structures were labeled by TRITC-phalloidin, a specific agent for staining actin microfilaments, and identified as having 9 nm diameters by negative staining. These results indicated that PEAc1 preserved the essential characteristics of actin even with His-tag and GFP fusion, suggesting a promising potential to use GFP fusion protein in obtainning soluble plant actin isoform to analyze its physical and biochemical properties in vitro. The PEAc1-GFP was also expressed in tobacco BY2.cells, which offers a new pathway for further studying its distribution and function in vivo.

关键词： actin isoform polymerization DNase I inhibition myosin Mg-ATPase activation expression.

Involvement of NADPH oxidase NtrbohD in the rapid production of H2.2.induced by ABA in cultured tobacco cell line BY-2

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Progress in Natural Science:Materials International 2008年第3期18卷 267-271页

作者： Fushun Hao a,b, Jinguang Zhang a, Zhonglian Yu a, Jia Chen a, a State key laboratory of {2. physiology and biochemistry, College of Biological Science, china Agricultural university, beijing 100094, china b Henan key laboratory of {2. Stress Biology, College of Life Science, Henan university, Kaifeng 475004, china State key laboratory of {2. physiology and biochemistry College of Biological Science China Agricultural University Beijing 100094 China Henan key laboratory of {2. Stress Biology College of Life Science Henan University Kaifeng 475004 China

The mechanisms for the production of hydrogen peroxide (H2.2. induced by abscisic acid (ABA) were investigated in suspension culture cells of tobacco BY-2.cells. The results showed that the immediate generation of H2.2. which was mainly derived from super- oxide dismutase-catalyzed dismutation of superoxide radical, was significantly induced by ABA. Furthermore, treatment of the cultured tobacco cells with ABA resulted in a time-dependent quick increase in plasma membrane (PM) NADPH oxidase activity, which coin- cided on time and magnitude with the elevation in ABA-induced accumulation of H2.2. Moreover, these enhanced effects were pro- nouncedly inhibited by two NADPH oxidase inhibitors, diphenylene iodonium and imidazole, suggesting that PM NADPH oxidase is involved in the rapid accumulation of H2.2.in cultured tobacco cells. In addition, analysis of the expression level of NtrbohD, a PM NADPH oxidase gene in tobacco, by RT-PCR and protein gel blot revealed that the gene at both mRNA and protein levels was upregulated by ABA, indicating that NtrbohD participates in the ABA-stimulated rapid production of H2.2.in tobacco culture cells. Taken together, these findings suggest that ABA induces the rapid accumulation of reactive oxygen species via NADPH oxidase in sus-pension culture cells of tobacco, and that NADPH oxidase and H2.2.appear to be important components in ABA signal transduction pathway in plants.

关键词： ABA Cultured tobacco cells H2.2 NADPH oxidase NtrbohD

Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages

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Chinese Science Bulletin 2003年第19期48卷 2068-2071页

作者： LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & WANG Xiangkun Department of plant Genetics and Breeding, china Agricultural {2., National key laboratory of Agrobiology, key laboratory of Crop Genetic Improvement and Genome of the ministry of agriculture, beijing 100094, china Department of plant Genetics and Breeding China Agricultural University National Key Laboratory of Agrobiology Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture China

An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2.(GC2., was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5.

关键词：耐寒性水稻开花期播种设备回交杂种 QTL分析数量特点轨迹

Analysis of genome-wide gene expression in root of wheat hybrid and its parents using Barleyl GeneChip

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Progress in Natural Science:Materials International 2006年第7期16卷 712-720页

作者： ZHANG Yinhong, NI Zhongfu, YAO Yingyin, ZHAO Jun and SUN Qixin (Department of plant Genetics & Breeding and State key {2. for Agrobiotechnology, key {2. of Crop Genomics and Genetic Improvement, ministry of agriculture / beijing key {2. of Crop Genetic Improvement, china Agricultural university,{12., china) [a]Department of plant Genetics & Breeding and State key {2. and Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing China

Previous studies indicated that differential gene expression between hybrids and their parents is responsible for the hct-crosis. In this paper, we report genome-wide gene expression analysis in roots of a highly heterotic inter-specific hybrid 3338/2.63 and its parental inbreds using Barley GeneChip. A total of 1187 genes displayed difference in gene expressions between hybrid 3338/2.63 and its parents, and they can be clustered into eight differential expression patterns. Further analysis revealed that among these 1187 genes, 975 genes showed high sequence similarity to the GenBank entries, and represented diverse functional categories, such as metabolism, cell growth and maintenance, signal transduction, response to stress, transcription regulation and others. Fourteen genes were selected for RT-PCR analysis and expression patterns of 9 (64. 2.% ) genes were confirmed. Remarkably, 380 differentially expressed genes could be mapped on the Chinese Spring deletion bins, and with the number of genes in seven homoeologous groups being 158, 148, 12., 140, 132. 94 and 12. respectively. It is concluded that a combination of systematic identification of differentially expressed genes with comparative mapping would provide further insight into understanding of molecular basis of heterosis.

关键词： GeneChip gene expression heterosis hybrid wheat.