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High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2., Libin DENG1#, and Changqing ZENG1,2. The institute of Genetic and Developmental Biology, the chinese {2. of sciences, beijing 100101, China 2.4} Genomics institute, the chinese {2. of sciences, beijing 101300, China The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing Genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 2.–30 bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3 were genotyped in a sample of 90 individuals, 4792.sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

Discovery of low grade metamorphic volcanic rock sheets within UHP in Dabie Mts. and its implications

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chinese Science Bulletin 1997年第14期42卷 1199-1203页

作者： DONG Shuwen, WANG Xiaofeng and HUANG Dezhi1. chinese academy of geological sciences (CAGS), beijing 10003., China 2. {2. of Geomechanics of CAGS, beijing 100081 China 3. Anhui {2. of geological sciences, Hefei 2.0001 chinese academy of {2. sciences (CAGS) Beijing 100037 China

THE ultrahigh-pressure metamorphic zone (UHP), outcropping in the central and southernDabie Mts. with an area of about 1000 km^2. may be the largest one in the world. The forma-tion and exhumation mechanism of the UHP rocks become a hot point in the study of continen-tal orogen. The first finding of the Shiqiao structural window, which is composed of low

关键词： ultrahigh-pressure metamorphic zone (UHP) low grade metamorphic rock sheets Dabie Mts.

Effective solutions to a global 3D visual system in networking environments

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Science China Earth sciences 2005年第11期48卷 2032-2039页

作者： ZHANG Liqiang1,2. ZHANG Yan3, YANG Chongjun2. LIU Suhong1, REN Yingchao2. RUI Xiaoping2.& LIU Donglin2.1. State Key Laboratory of Remote Sensing sciences, School of Geography and Remote Sensing, beijing Normal University, beijing 100875, China 2. State Key Laboratory of Remote Sensing sciences, institute of Remote Sensing Applications, chinese academy of sciences, beijing 101001, China 3. institute of Geographical sciences and Natural Resources Research, chinese academy of sciences, beijing 101001, China 1. State Key Laboratory of Remote Sensing sciences School of Geography and Remote Sensing Beijing Normal University 100875 Beijing China 2. chinese academy of {2. State Key Laboratory of Remote Sensing SciencesInstitute of Remote Sensing Applications 101001 Beijing China 3. institute of Geographical sciences and Natural Resources Research Chinese Academy of Sciences 101001 Beijing China

The development of Web-based global 3D visual system has made progresses. However, there exist few mature and effective ways for managing, transmitting and visualizing massive spatial data. Based on the related work, the paper illustrates the ellipsoidal quadtree technique for rapid access multi-scale and multiple level geographical data, integrates the streaming with level-of-detail rendering method for transmitting the data on the network, and im-plements large-scale terrain surface simplification using M-band wavelet transforms and mul-tiresolution triangulations. We fulfil a web-based global terrain visual system using COM com-ponents on the basis of the above techniques. The system has a good prospect in the military simulation and city plans.

关键词： massive spatial data, streaming transmission, system architecture, M-band wavelet

Debris Flow Hazard Assessment Based on Support Vector Machine

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Wuhan University Journal of Natural sciences 2006年第4期11卷 897-900页

作者： YUAN Lifeng 1, 2., ZHANG Youshui 3 1. institute of Mountain Hazards and Environment, chinese {2. of sciences, Chengdu 610041, Sichuan, China 2. Graduate University of chinese {2. of sciences, beijing 100049, China 3. institute of Geographical sciences and Natural Resources Research, chinese {2. of sciences, beijing 100101, China institute of Mountain Hazards Environment Chinese Academy of Sciences 610041 Chengdu Sichuan China Graduate University of chinese academy of {2. 100049 Beijing China institute of Geographical sciences Natural Resources Research Chinese Academy of Sciences 100101 Beijing China

Seven factors, including the maximum volume of once flow , occurrence frequency of debris flow , watershed area , main channel length , watershed relative height difference , valley incision density and the length ratio of sediment supplement are chosen as evaluation factors of debris flow hazard degree. Using support vector machine （SVM） theory, we selected 2.9 basic data of 37 debris flow channels in Yunnan Province as learning samples in this study. We create a debris flow hazard assessment model based on SVM. The model was validated though instance applications and showed encouraging results.

关键词： debris flow hazard assessment support vector machine (SVM)

Isotopic feature and uranium dating of the volcanic rocks in the Okinawa Trough

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中国科学：地球科学英文版 2006年第4期49卷 375-383页

作者： HUANG Peng1,2. LI Anchun1, HU Ningjing2.3, FU Yongtao1& MA Zhibang4 1. institute of Oceanology, chinese {2. of sciences, Qingdao 2.6071, China 2. Graduate School of the chinese {2. of sciences, beijing 10003., China 3. institute of Soil Science, chinese {2. of sciences, Nanjing 2.0008, China 4. institute of geology and Geophysics, chinese {2. of sciences, beijing 10002., China 1. institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 2. Graduate School of the chinese academy of {2. Beijing 100039 China 3. institute of Soil Science Chinese Academy of Sciences Nanjing 210008 China 4. institute of geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

Volcanic rocks from the northern and middle Okinawa Trough were dated by ura- nium-series dating method. Differential fractions using magnetic procedure were designed to separate samples. New report on the ages and isotopic data of rocks in the northern trough (especially black pumice) was discussed. Based on the uranium dates and Sr-Nd isotopic ratio, magmatic evolution process of the Okinawa Trough was noted. Firstly, there have been wide silicic volcanic activities in the Okinawa Trough from late Pleistocene to present, and the volcanic rocks can be divided into three subgroups. Secondly, magma generally came from PREMA source area under the Okinawa Trough. Magmatic evolution in the northern trough was similar to the middle, but different to the south. Finally, volcanic activities indicated that opening of the southern Okinawa Trough did not happen due to the collision between Luson Arc and Eurasian Plate until the early Pleistocene.

关键词： Okinawa Trough, black pumice, uranium-series dating, Sr-Nd isotopic ratio, magmatic evolution.

Si-and alkali-rich melt inclusions in minerals of mantle peridotites from eastern China:Implication for lithospheric evolution

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Science China Earth sciences 2006年第1期49卷 43-49页

作者： FAN Qicheng1, SUI Jianli2. XU Ping2. LI Ni1, SUN Qian1 & WANG Tuanhua1 1. institute of geology, China Seismological Bureau, {2. 10002., China 2. institute of geology and Geophysics, chinese academy of sciences, {2. 10002., China 1. institute of geology China Seismological Bureau Beijing 100029 China 2. institute of geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

Minerals of spinel- and garnet-facies mantle xenoliths entrained in Cenozoicbasalts from eastern China (North China, Northeastern China and Southeastern China coastal area)contains lots of melt inclusions. Studies on these melt inclusions show that the glass inclusionsare rich in SiO_2.(60%—68%) and alkalis (K_2.+Na_2. = 5%—11%, especially for K_2.) as well asvolatiles such as H_2. and CO_2.(2.—7%), which belong to dacites and andesites of the high-K calcicalkali series rocks with few shoshonites. High Al and Ca diopside in melt inclusion is the productof melt crystallization at high temperature and pressure, rather than the product ofdevitrification. Results show that these K-rich (in general K_2. > 3%) intermediate-acidic silicatemelt inclusions have characteristics of continent without a genetical link to host basalts and theirphenocrystic minerals. Thus, these trapped melt inclusions represent melts of Mesozoic lithosphericmantle-crust interaction and imply that the continental lithospheric mantle beneath eastern Chinahad undergone fragmentation and recreation processes during the Mesozoic and Cenozoic periods. Thisresult undoubtly provides important implication for the evolution of sub-continental lithospherebeneath eastern China. We propose that these Si- and alkalis-rich melts should be responsible forthe mantle chemical heterogeneity underneath eastern China.

关键词： Si- and alkali-rich melt inclusion, mantle peridotite, lithospheric evolution, eastern China.

Research progress in the inner surface modification of metal tubes by plasma processing

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Progress in Natural Science:Materials International 2006年第11期16卷 1119-1126页

作者： ZHANG Guling~ 1,2.**, WU Xingfang~1, GU Weichao~2. CHEN Guangliang~2. FENG Wenran~2. NIU Erwu~2. LI Li~2. L Guohua~2. CHEN Huan~2. FAN Songhua~2. LIU Chizi~2.and YANG Size~2.1. School of Material Science and Engineering, University of Science and Technology beijing, beijing 100083, China 2. beijing National Laboratory for Condensed Matter Physics, institute of Physics, chinese academy of sciences, beijing 100080, China) School of Material Science and Engineering University of Science and Technology Beijing Beijing 100083 China beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The recent progress in inner surface hardening of tubes by plasma processing is summerized. Several techniques of inner surface plasma source ion implantation and deposition are introduced, and their advantages and disadvantages are discussed. The basic principles, technical features and new progress of inner surface plasma source ion implantation methods for metal tubes, which were developed in our laboratory, are described in detail. And perspectives of the future technical development for inner surface ion implantation of tubes are presented.

关键词： PSII metal tubes inner surface surface modification.

Plus cupping degrees do not form an ideal

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Science in China(Series F) 2004年第5期47卷 635-654页

作者： LI Angsheng1,2.& ZHAO Yicheng1 1. institute of Software,chinese {2. of sciences,beijing 100080,China 2. School of Information sciences,beijing Normal University,beijing 100871,China 1. institute of Software Chinese Academy of Sciences 100080 Beijing China 2. School of Information sciences Beijing Normal University 100871 Beijing China

A computably enumerable (c.e.,for short)degree a is called plus cupping,if every c.e. degree x with 0 详细信息

A computably enumerable (c.e.,for short)degree a is called plus cupping,if every c.e. degree x with 0

关键词： computably enumerable set Turing degree definability.

Northernmost paleo-tethyan oceanic basin in Tibet:Geo-chronological evidence from ^(40)Ar/^(39)Ar age dating of Dur'ngoi ophiolite

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chinese Science Bulletin 2001年第14期46卷 1203-1205页

作者： CHEN Liang, SUN Yong, PEI Xianzhi, GAO Ming, FENG Tao, ZHANG Zhongqing & CHEN Wen1. Laboratory of Continental Dynamics, Northwest University, Xi’an 710069, China 2. Department of geology, Northwest University, Xi’an, 710069, China 3. institute of geology, chinese academy of geological sciences, beijing, 10003., China Laboratory of Continental Dynamics Northwest University Xi’an China Department of geology Northwest University Xi’an China institute of geology Chinese Academy of Geological Sciences Beijing China

Whole rock 40Ar/39Ar age dating has been conducted on a basalt sample from Dur’ngoi ophiolite, Qinghai Province, which was reported to be the northernmost pa-leo-tethyan oceanic basin in Tibet. A high temperature pla-teau age (345.3±7.9 Ma) with an isochorn age (336.6±7.1 Ma) has been obtained, representing the eruption time of oceanic crust Considering related geological settings, the new age provides constraints on the northernmost pa-leo-tethyan suture zone in Tibet and the tectonic evolution of Paleo-tethys in Northeast Tibet and adjacent areas.

关键词： Dur’ngoi ophlolite 40Ar -39Ar age Paleo-tethys.