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Application of RAPD technology for identification in three different stocks of Penaeus chinensis

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Acta Oceanologica Sinica 2003年第4期22卷 629-634页

作者： Liu Zhenhui1, Kong Jie 2. Liu Ping2. Deng Jingyao2.1. College of Marine Biology Science, Ocean University of china, qingdao 2.6003, china 2. Huanghai Sea Fisheries Research institute, Chinese academy of Fishery sciences, qingdao, 2.6071. china Ocean Univ china Coll Marine Biol Sci Qingdao 266003 Peoples R China Chinese Acad Fishery Sci Huanghai Sea Fisheries Res Inst Qingdao 266071 Peoples R China

The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coastal waters of the Korea Peninsula, H was from the Huanghai Sea-Bohai Sea stock and S was from the health cultured stock in Jimo, Shandong Province, china. Three genetic markers, F-02.540), OPN-13(800) and OPN-2.(2.00), which could differentiate the three different stocks, have been reported. The basic data for marker assisted selection (MAS) on genetic breeding of Penaeus chinensis are provided.

关键词： Penaeus chinensis RAPD technology genetic marker

Genesis of ^(2.0)Th excess in basalts from mid-ocean ridges and ocean islands:Constraints from the global U-series isotope database and major and rare earth element geochemistry

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Science china Earth sciences 2010年第10期53卷 1486-1494页

作者： ZHANG GuoLiang1,2.& ZENG ZhiGang1 1 Key Laboratory of Marine Geology and Environment,institute of oceanology,Chinese {2. of sciences,qingdao 2.6071.china 2.Graduate University of Chinese {2. of sciences,Beijing 100049,china 1. Key Laboratory of Marine Geology and Environment Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China

Based on 2.0Th-2.8U disequilibrium and major element data from mid-ocean ridge basalts(MORBs) and ocean island basalts(OIBs),this study calculates mantle melting parameters,and thereby investigates the origin of 2.0Th excess.(2.0Th/2.8U) in global MORBs shows a positive correlation with Fe8,Po,Na8,and Fmelt(Fe8 and Na8 are FeO and Na2. contents respectively after correction for crustal fractionation relative to MgO = 8 wt%,Po=pressure of initial melting and Fmelt=degree of melt),while 2.0Th excess in OIBs has no obvious correlation with either initial mantle melting depth or the average degree of mantle ***,compared with the MORBs,higher(2.0Th/2.8U) in OIBs actually corresponds to a lower melting *** suggests that the 2.0Th excess in MORBs is controlled by mantle melting conditions,while the 2.0Th excess in OIBs is more likely related to the deep garnet *** vast majority of calculated initial melting pressures of MORBs with excess 2.0Th are between 1.0 and 2.5 GPa,which is consistent with the conclusion from experiments in recent years that DU>DTh for Al-clinopyroxene at pressures of >1.0 *** initial melting pressure of OIBs is 2.2.3.5 GPa(around the spinel-garnet transition zone),with their low excess 2.6Ra compared to MORBs also suggesting a deeper mantle ***,excess 2.0Th in MORBs and OIBs may be formed respectively in the spinel and garnet stability *** addition,there is no obvious correlation of K2./TiO2.with(2.0Th/2.8U) and initial melting pressure(Po) of MORBs,so it is proposed that the melting depth producing excess 2.0Th does not tap the spinel-garnet transition *** and MORBs in both(2.0Th/2.8U) vs.K2./TiO2.and(2.0Th/2.8U) *** plots fall in two distinct areas,indicating that the mineral phases which dominate their excess 2.0Th are ***/Yb-Ce curves of fast and slow ridge MORBs are similar,while,in comparison,the Ce/Yb-Ce curve for OIBs shows more influence from *** mechanism

关键词： excess 2.0Th clinopyroxene garnet spinel-lherzolite mid-ocean ridge ocean island

Energy distributions of the large-scale horizontal currents caused by wind in the baroclinic ocean

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Science china Earth sciences 2005年第12期48卷 2267-2275页

作者： ZHOU Lei1, TIAN Jiwei1 & WANG Dongxiao2.1. Physical Oceanography Laboratory, Ocean University of china, qingdao 2.6003, china 2. Key Laboratory of Tropical Marine Environmental Dynamics, South china Sea institute of oceanology, Chinese academy of sciences, Guangdong 510301, china 1. Physical Oceanography Laboratory Ocean University of China 266003 Qingdao China 2. Key Laboratory of Tropical Marine Environmental Dynamics South China Sea Institute of Oceanology Chinese Academy of Sciences 510301 Guangdong China

Ocean current data for nearly 3 months in the South china Sea (SCS), combined with the NCEP/NCAR reanalysis wind data, are analyzed. The results indicate that the wind en-ergy enters the upper mixed layer in a wide continuous frequency band. In addition, the interac-tion between the low-frequency wind anomaly and the low-frequency current anomaly is the most ‘effective’ way for the energy input from the wind to the upper ocean. However, only the inertial and the near inertial energy propagate downwards through the upper mixed layer. The down-ward-propagating energy is distributed into the barotropic currents, the baroclinic currents and each mode of the baroclinic currents following the normal distributions. The energy change ratios between the barotropic motion to the baroclinic motion induced by the wind present a normal distribution of N (0.02.2. 0.3947). The energy change ratios of the first 4 baroclinic modes to the whole baroclinic currents also follow the normal distributions. The first baroclinic mode follows N (0.2.2., 0.1872., the second N (0.1979, 0.1504), the third N (0.1331, 0.1633), and the fourth N (0.0650, 0.1540), respectively.

关键词： wind anomaly, ocean current anomaly, energy transportation, wide frequency band, near-inertial fre-quency, energy distribution.

Sporopollen analysis of Core B10 in the southern Yellow Sea and the reflected characteristics of climate changes

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Chinese Science Bulletin 2003年第B06期48卷 42-48页

作者： FU Mingzuo~(1,2.3), LI Zhen~1, XU Xiaowei~1 & SHl Xuefa~1 1. Key Laboratory of Marine Sedimentology and Environmental Geology, First institute of Oceanography. State Oceanic Administration, qingdao 2.6061. {2. 2. institute of oceanology, Chinese academy of sciences, qingdao 2.6071. china 3. Graduate School, Chinese academy of sciences, Beijing 100039. {2. 1. Key Laboratory of Marine Sedimentology and Environmental Geology First institute of Oceanography State Oceanic Administration 266061 Qingdao China 2. institute of oceanology Chinese Academy of Sciences 266071 Qingdao China 3. Graduate School Chinese Academy of Sciences 100039 Beijing China

Eight sporopollen zones have been divided based on the results of high-resolution sporopollen analysis of Core B10 in the southern Yellow Sea. Based on the results along with ^14C datings and the subbottom profiling data,climatic and environmental changes since the last stage of late Pleistocene are discussed. The main conclusions are drawn as follows: (1) the vegetation evolved in the process of coniferous forest-grassland containing broad-leaved trees→coniferous and broad-leaved mixed forest→coniferons and broad-leaved mixed forest-grassland prevailed by coniferous trees→coniferous and broad-leaved mixed forest-grassland containing evergreen broad-leaved trees→coniferons and broad-leaved mixed forest-grassland prevailed by broadleaved trees→deciduous broad-leaved forest-meadow containing evergreen broad-leaved trees→coniferous and broadleaved mixed forest-grassland prevailed by broad-leaved trees→coniferous and broad-leaved mixed forest containing evergreen broad-leaved trees; (2. eight stages of climate changes are identified as the cold and dry stage, the temperate and wet stage, the cold and dry stage, the warm and dry stage, the temperate and wet stage, the hot and dry stage, the temperate and dry stage, then the warm and dry stage in turn; (3) the sedimentary environment developed from land,to littoral zone, to land again, then to shore-neritic zone; and (4) the Yellow Sea Warm Current formed during early-Holocene rather than Atlantic stage.

关键词：中国黄海气候变化碳14年代测定底基压型数据更新世沉积环境大西洋

SUSPENDED SEDIMENT TRANSPORT IN THE OFFSHORE NEAR YANGTZE ESTUARY

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Journal of Hydrodynamics 2008年第3期20卷 373-381页

作者： CHEN Bin WANG Kai institute of oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate University of the Chinese academy of sciences Beijing 100039 China

Based on the Estuarine, Coastal and Ocean Modeling System with Sediments （ECOMSED） model, a 3-D hydrodynamic-transport numerical model was established for the offshore area near the Yangtze Estuary in the East china Sea .The hydrodynamic module was driven by tide and wind. Sediment module included sediment resuspension, transport and deposition of cohesive and non-cohesive sediment. The settling of cohesive sediment in the water column was modeled as a fimction of aggregation （flocculation） and deposition. The numerical results were compared with observation data for August, 2.06. It shows that the sediment concentration reduces gradually from the seashore to the offshore area. Numerical results of concentration time series in the observation stations show two peaks and two valleys, according with the observation data. It is mainly affected by tidal current. The suspended sediment concentration is related to the tidal current during a tidal cycle, and the maximum concentration appears 1 h-4 h after the current maximum velocity has reached.

关键词： Yangtze Estuary sediment concentration sediment transport

Distribution of molluscan remains in the sediment of the Chukchi Sea and its vicinity, the Arctic

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Acta Oceanologica Sinica 2003年第4期22卷 607-619页

作者： Gao Aiguo 1,2. Xu Fengshan3, Sun Haiqing 1,2. Li Lon1 1. First institute of Oceanography, State Oceanic Administration, qingdao 2.6061,{2.. 2. Key Laboratory of Marine Sedimentology and Environmental Geology, State Oceanic Administration, qingdao 2.6061,{2. 3. institute of oceanology, Chinese academy of sciences, qingdao 2.6071.{2. State Ocean Adm Inst Oceanog 1 Qingdao 266061 Peoples R China State Ocean Adm Key Lab Marine Sedimentol & Environm Geol Qingdao 266061 Peoples R China Chinese Acad Sci Inst Oceanol Qingdao 266071 Peoples R China

The result of an analysis of mollusca remains collected from the Chukchi Sea, Beaufort Sea and Bering Sea in the First Chinese National Arctic Research Expedition, from July to September, 1999 is presented. Seventeen species of mollusca have been identified, which belong to two classes: Bivalvia and Gastropoda. The compositions of the mollusca are very simple. According to the distribution pattern two groups may be distinguished among molluscan species. The Pan-Arctic and circumboreal group comprises Nuculana pernula, ***, Nucula bellotii, Astarte montagui, Seripes groenlandicus, Macoma calcarea, M. moesta alaskana, Liocyrna fluctuosa, Mya pseudoarenaria and Turritella polaris. Three species, Cyclocardia crebricostata, Trichotrois coronata and Argobuccinum oregonense are components of the Pan-Arctic and Pacific boreal group. With regard to feeding habits, detritus feeders dominate. There are 7 species of detritus feeders, i.e., Nuculana pernula, N. radiata, Nucula bellotii, Macoma calcarea, M. moesta alaskana, Macoma sp. and Trichotropis coronata. Detritus feeders are dominant with regard to the numbers of species as well as to the frequency of occurrence. Macoma calcarea is the most abundant species.

关键词： Chukchi Sea Beaufort Sea Bering Sea molluscan remains

Effects of artificial regulations of rotifers n-3 HUFA content on the growth and survival of larval black sea bream ( Sparus macrocephalus)

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Chinese Science Bulletin 1997年第13期42卷 1130-1132页

作者： LIU Jingke and LEI Jilin1. institute of oceanology, Chinese {2. of sciences, qingdao 2.6071. china 2.Yellow Sea Fishery Research institute qingdao 2.6003, china institute of oceanology Chinese Academy of Sciences Qingdao 266071 China

STUDIES on the nutrition requirement of larval fish have made some progress abroad, but therehave been no reports on such studies in china. It was determined recently that n-3 HUFA (es-pecially 2.: 6(n-3) (DHA) and 2.:5 (n-3) (EPA)) is essential for larval seafish growth. Thehigh content of DHA in seafish eggs and the low content of DHA in rotifers show that rotifersas live food cannot satisfy the n-3 HUFA requirement of larval seafish. Some scientists ob-

关键词： rotifer (Brachionus phicatilis) highly unsaturated fatty acid (HUFA) larval black sea bream.

Some observational results of sea storm current

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Acta Oceanologica Sinica 2001年第2期20卷 299-308页

作者： Xiu Richen, Liu Aiju, Ye Hesong, Gu Yuhe, Xu Lanying (1. First institute of Oceanography, State Oceanic Administration, qingdao 2.6061, {2. 2. institute of oceanology, Chinese academy of sciences, qingdao 2.6071. china) First institute of Oceanography State Oceanic Administration Qingdao 266061 China

INTRODUCTIONDr. Hollister, a marine geologist of the Woods Hole Oceanographic Institution, first pointed out that there was ocean storm current in the ocean. He found out the wavy texture in the seabed core samples, and suggested that this wavy texture was caused by the high speed sea current in remote antiquity. He then suggested a bold hypothesis that there existed a benthic storm current near the ocean bottom, and presented this hypothesis at the IUGG conference held at San Francisco in 1963. Unfortunately, the attention was not drawn to the hypothesis at the conference, and the hypothesis was criticized as a sheer nonsense.

关键词： Sea storm current tidal storm current chaotic motion

Effects of diatoms on reproduction and growth of marine copepods

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Progress in Natural Science:Materials International 2004年第10期14卷 1-7页

作者： ZHANG Guangtao 1,2. and SUN Song 1 (1.Key Laboratory of Marine Ecology&Environment sciences,institute of {2.,Chinese academy of sciences,qingdao2.6071.china 2.Graduate School,Chinese academy of sciences,Beijing100039,china) Chinese Acad Sci Inst Oceanol Key Lab Marine Ecol & Environm Sci Qingdao 266071 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

Two hot spots in marine ecology,deleterious effects of diatoms and feeding selectivity of copepods,as well as new progress on these two issues achieved in the recent ten years,are *** two issues are considered correlated *** and their metabolites can induce deleterious effects on growth,reproduction and development of copepods,including increase of mortality and decrease of egg production,hatching and growth *** negative effects,resulting from either chemical toxin or nutritional defi-ciency,can be conquered in natural environments by diverse *** is therefore concluded that deleterious effects of diatoms observed in laboratory or during blooming period are only a special case that accommodation of feeding strategy of copepods is *** under-stand their feeding strategy in natural environments is a prerequisite to explaining the mechanisms of deleterious effects caused by diatoms,and makes it possible to re-evaluate the energy flow in marine ecosystems.

关键词： diatoms copepod feeding strategy reproduction deleterious effects