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High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2., Libin DENG1#, and Changqing ZENG1,2. The institute of Genetic and Developmental Biology, the chinese {2. of sciences, beijing 100101, china 2.4} Genomics institute, the chinese {2. of sciences, beijing 101300, china The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing Genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 2.–30 bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3 were genotyped in a sample of 90 individuals, 4792.sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

MODELLING AND NUMERICAL SOLUTIONS OF A GAUGE PERIODIC TIME DEPENDENT GINZBURG-LANDAU MODEL FOR TYPE-Ⅱ SUPERCONDUCTORS

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Journal of Computational Mathematics 1997年第4期15卷 365-384页

作者： Zhi-ming Chen (institute of Mathematics, chinese {2. of sciences, beijing, china) 中科院数学所北京

In this paper we seek the solutions of the time dependent Ginzburg-Landau model for type-Ⅱ superconductors such that the associated physical observables are spatially periodic with respect to some lattice whose basic lattice cell is not necessarily rectangular. After appropriately foring the gange, the model can be formulated as a system of nonlinear parabolic partial differential equations with quasi-periodic boundary conditions. We first give some results concerning the existence, uniqueness and regularity of solutions and then we propose a semiimplicit finite element scheme solving the system of nonlinear partial dmerential equations and show the optimal error estimates both in the L2.and energy *** also report on some numerical results at the end of the paper.

关键词： Math QT MODELLING AND NUMERICAL SOLUTIONS OF A GAUGE PERIODIC TIME DEPENDENT GINZBURG-LANDAU MODEL FOR TYPE SUPERCONDUCTORS

Negative Haloes and Geochemical Field Systems of Hydrothermal deposits

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Acta Geologica Sinica(English Edition) 1993年第2期67卷 195-209页

作者： Ji Kejian, Wang Liben, Lu Fengxiang, Wang Wuyi and Zhang Jianhua institute of mineral {2., chinese academy of geological sciences, beijing institute of mineral {2. Chinese Academy of Geological Sciences Beijing

Primary haloes of hydrothermal deposits were previously considered as positive haloes. Although it has been recently suggested that primary haloes incude positive and negative ones, almost all the research work on primary haloes has still focused on positive haloes for various reasons. In order to understand the source of ore substances a number of large hydrothermal deposits related to magmatism including porphyry, skarn and vein type deposits have been studied. It has been found that negative haloes exist on the peripheries of positive haloes of a mineral deposit. On the basis of the study the authors propose the concept of the geochemical field system of ore-forming elements of hydrotherreal deposits, i. e., with the orebody as the centre outwards there occur the mineralized field, positive halo field, depleted field and background field successively.

关键词： hydrothermal deposit negative halo geochemical field system

mineralogy and Mode of Occurrence of Gold, Silver and Bismuth of the Caijiaying Lead-Zine-Silver Deposit, Hebei Province

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Acta Geologica Sinica(English Edition) 1991年第4期65卷 371-385,461页

作者： Huang Dianhao Ding Xiaoshi Wu Chengyu Zhang Changjiang institute of mineral {2., chinese academy of geological sciences, beijing geological Party No.3, Hebei Bureau of Geology and mineral Resources, Zhang jiakou, Hebei Fei Zhenbi institute of mineral {2. Chinese Academy of Geological Sciences Beijing China

The deposit under study is a hydrothermal filling-metasomatic vein type lead-zinc-silver deposit, in whichgold and silver can be recovered as by-products. These metals mainly occur as microgranular native gold,electrum, stephanite, acanthite, pyraragyrite, freibergite, and native silver. Gold minerals tend to be associatedwith galenobismutite, native bismuth and unnamed Bi_2.e. They are either enclosed in pyrite, marmatite,iron-bearing sphalerite and galens or fill the microfissures of these minerals. Silver minerals usually occur incleavages or fissures of galena, marmatite and pyrite, but are not associated with gold and bismuth minerals.Gold and silver mineralizations occurred later than lead and zinc, while the silver mineralization was precededby that of gold.

关键词： mineralogy and Mode of Occurrence of Gold Hebei Province

Zijinshan-Bitian Cu-Au-Mo orefield, SW Fujian——An example of gold-rich PCS zoning

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chinese Science Bulletin 1999年第S2期44卷 134-135页

作者： QI Jianzhong~1, LI Zhaonai~2. ZHANG Dequan~3, FANG Xiheng~4 and XIAO Huiliang~1 1. Nanjing institute of Geology and mineral Resources,{2., Nanjing 2.0016, china 2. institute of Geology,{2., beijing 10003., china 3. institute of mineral deposits,{2., beijing 10003., china 4. institute of Geology, Ministry of Nuclear Industry, beijing 100013, china Nanjing institute of Geology and mineral Resources Chinese Academy of Geological Sciences Nanjing 210016 China institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China institute of mineral {2. Chinese Academy of Geological Sciences Beijing 100037 China institute of Geology Ministry of Nuclear Industry Beijing 100013 China

THE Zijinshan-Bitian Cu-Au-Mo orefield is spread to north of the Shanghang County, SW *** geological structure it is located at the plunging end of NE-trending Xuanhe anticlinorium, in the NE rim ofthe NW trending Shanghang volcano-sedimentary basin. Orefield basement is composed of the Archean-Middle Proterozoic low-grade metamorphic rock formations, containing marine basic metavolcanic sequenses. Lower Cretaceous subaerial volcanic rocks of andesite-trachy andesite-rhyolite composition fill inthe volcano-sedimentary basin. Pre-mineralization granitic batholith dated 145Ma（Rb-Sr）and 157Ma（U-Pb） occupies the axial part of the plunging anticlinorium, which was regarded as crust-remelting typegranitoids. mineralization-affiliated subvolcanic dacite porphyrite, co-volcanic granodiorite and quartzdiorite porphyrite （102.2.Ma（Rb-Sr）, Sr1 = 0.708 9） belong to syntactic type in genesis and

关键词： gold-rich porphyre copper system zoning Fujian Zijinshan.

The possible contribution of a mantle-derived fluid to the Ningwu porphyry iron deposits—Evidence from carbon and strontium isotopes of apatites

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Progress in Natural Science:Materials International 2008年第2期18卷 167-172页

作者： Jinjie Yu ,Jingwen Mao,Changqing Zhang institute of mineral Resources,{2. academy of geological Science,beijing 10003.,china institute of mineral Resources Chinese Academy of Geological Science Beijing 100037 China

Apatite is a characteristic mineral for the iron deposits in the Ningwu region. Apatite shows a range of d13CPDB values from à9.6& to à0.6&,and most of them with the d13CPDB values distributed in a range of mantle carbon (i.e.,à2. to à9&),except for samples T-06-1 and J-06-3. Apatites in the Ningwu porphyry iron deposits yield a narrow variation range of 87Sr/86Sr values from 0.70632. to 0.707577,similar to those of the volcanic and subvolcanic rocks and higher than that of a typical mantle-derived magmatic apatite (i.e.,about 0.7040),indicating that formation of the apatites was involved with crust material contamination. d13CPDB and 87Sr/86Sr values of the apatite indicate a possible contribution of mantle-derived fluid and material to formation of the Ningwu porphyry iron deposits,supported by regional geology,regional gravities and geochemistry of the volcanic and subvolcanic rocks in the Ningwu region.

关键词： Apatite 87Sr/86Sr ratios Fluid inclusion d13C Mantle fluid and material

Isotopic compositions of small shelly fossil Anabarites from Lower Cambrian in Yangtze Platform of South china: Implications for palaeocean temperature

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Progress in Natural Science:Materials International 2007年第10期17卷 1185-1191页

作者： Chen Yongquan1,2. Jiang Shaoyong1, Ling Hongfei1, Yang Jinghong1 and Wan Defan3(1. State Key Laboatory for mineral deposits Research and Center for Marine Geochemistry Research, Department of Earth {2., Nanjing University, Nanjing 2.0093, china 2. Tarim Oilfield Company Exploration & Development Research institute, Ku’erle 841000, china 3. institute of mineral Resources, chinese academy of geological sciences, beijing 10003., china) State Key Laboatory for mineral deposits Research and Center for Marine Geochemistry Research Department of Earth Sciences Nanjing University Nanjing 210093 China arim Oilfield Company Exploration & Development Research institute Ku’erle 841000 China institute of mineral Resources Chinese Academy of Geological Sciences Beijing 100037 China

Anabarites belong to small shelly fossils (SSF), which occur widely in the Lower Cambrian strata of Yangtze Platform in South china. They are phosphate shell in composition and represent the earliest stage of the Cambrian bioradiation of Bilateria, the so-called "Cambrian Explosion". In this study, we attempted to separate Anabarites fossils from Lower Cambrian dolostones, and we obtained samples of both the fossils (SSF) and the granular phosphates (GP). Isotopic analyses were performed on samples of SSF, GP, and matrix dolostone (DH-2.). The results showed that the δ30Si values of the quartz filling in fossils celoms, and the siliceous materials in granular phosphates are -0.6‰ and -0.7‰, which is different from normal sedimentary siliceous rocks from the Lower Cambrian strata (0—0.7‰) as reported by Li et al., but is consistent with the data for siliceous rocks and cherts of submarine hydrothermal origin. It is likely that a later hydrothermal replacement could have taken place in the SSF-bearing sedimentary rocks. The oxygen isotope values of the phosphate of SSF and GP are 16.8‰ and 17.0‰, respectively. These are significantly higher than the Neoproterozoic phosphate ores (10.9‰—13.9‰) as reported by Ling et al., hence, late diagenesis and hydrothermal replacement may not have caused a significant change in the oxygen isotope compositions of the small shelly fossils, and the calculated temperatures (2..4—2..3℃) for palaeo-seawater using a SSF phosphate oxygen isotope thermometer are therefore considered here as the upper limit of seawater temperature in the Early Cambrian ocean of the Yangtze Platform.

关键词： Anabarites oxygen isotope silicon isotope paleo-temperature.

Carbon and Oxygen Stable Isotope Values and Microfossils at 41.4-4.5ka BP in Tai Co,Tibet,china,and Their Paleoclimatic Significance

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Acta Geologica Sinica(English Edition) 2011年第5期85卷 1036-1050+1052-1056+1051页

作者： ZHENG Mianping~(1,2,*),LIU Junying~(1,3),MA Zhibang~(1,4),WANG Hailei~(1,2) and MA Nina~(1,2) 1 MLR Key Laboratory of Saline Lake Resources and Environment,institute of mineral Resources,CAGS, beijing 10003.,china 2 Research and Development Center for Saline Lakes and Epithermal deposits,chinese academy of geological sciences,beijing 10003.,china 3 institute of Geology,chinese academy of geological sciences,beijing 10003.,china 4 Key Laboratory of Cenozoic Geology and Environment,chinese academy of sciences,beijing 100029, china MLR Key Laboratory of Saline Lake Resources and Environment Institute of Mineral Resources CAGS Beijing 100037 China Research and Development Center for Saline Lakes and Epithermal deposits Chinese Academy of Geological Sciences Beijing 100037 China institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Key Laboratory of Cenozoic Geology and Environment Chinese Academy of Sciences Beijing 100029 China *Correspondence: Mianping ZHENG *Correspondence: Corresponding author. E-mail: zhengmp2010@***

Two sedimentary sections were measured at Tai Co(Co means lake in Tibetan) in western Tibet,*** two sections are almost all composed of clay carbonate beds except in their lower parts where there are carbonized plants at>10 cm depths and dark-colored carbonate clay and clay at 50-70 cm depths,yielding abundant gastropods,ostracods,and *** carbon and oxygen stable isotope values of carbonate,ostracods,and charophytes and ecological features of microfossil communities indicate the following climatic change in the area from 41.4 to 4.5 ka BP:at 41.4-2..2.ka BP,the climate was relatively wet;at 2..2.2..5 ka BP,it was slightly warm-dry;at 2..5-2..5 ka BP,it was warm-wet;at 2..5-2..0 ka BP,it was slightly cold-wet;at 2..5-17.5 ka BP,it became cold abruptly and slightly wet,implying the climate of the last glacial maximum;at 17.5-16.0 ka BP,it was slightly cold-dry;at 16.0-11.8 ka BP,it was slightly warm-wet;at 11.8-10.4 ka BP,it was relatively cold-dry, roughly equivalent to the climate of the Younger Dryas,and at 10.4 ka BP,the temperatures began to rise again;at 10.4-9.4 ka BP,it was slightly warm-wet;at 9.4-8.5 ka BP,there occurred short warm-wet oscillations;at 8.5-7.9 ka BP,it was slightly dry-cold,representing a strong temperature-lowering and drying event in the postglacial stage;at 7.8-6.3 ka BP,it was slightly warm-wet;at 6.3-4.5 ka BP,the climate tended to be cold-dry.4.5 ka BP recorded the maximum aridity since the late part of the late Pleistocene.

关键词： sedimentary environment index paleoclimatic changes late Pleistocene-Holocene(41.4-4.5 ka BP) Tai Co Tibet

REE Geochemistry and mineralization Characteristics of the Zudong and Gnanxi Granites, Jiangxi Province

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Acta Geologica Sinica(English Edition) 1989年第2期63卷 139-157页

作者： Huang Dianhao, Wu Chengyu institute of mineral {2., chinese academy of geological sciences, beijing and Han Jiuzhu Southern Jiangxi geological Survey Party, Jiangxi Bureau of Geology and mineral Resources, Ganzhou Zhang Zhong min institute of mineral {2. Chinese Academy of Geological Sciences Beijing China

The Zudong and Guanxi granites are original rocks of the ion adsorption-type HREE and LREE deposits in weathering crust of granites. The ΣREE value and LREE/HREE ratio of the Zudong granite are 2.4 ppm and 0.81-0.2. respectively, and the average Y/ΣREE ratio is 35.8-54.5%. This is mainly due to magmatic crystallization and evolution and deuteric metasomatism (albitization, muscovitization and fluorite-doveritization). These alterations resulted in endogenic mineralizations of yttrium-group REE fluorine carbonates, silicates and arsenates. The Guanxi granite is characterized by LREE enrichment (the average LREE/HREE ratio is 2.43).

关键词： REE Geochemistry and mineralization Characteristics of the Zudong and Gnanxi Granites Jiangxi Province