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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2. Xiulan CHENG1, and Shijun QIAN1* 1institute of Microbiology, chinese academy of sciences, {2. 100080, china 2.enter of Mass Spectrometry, institute of Chemistry, chinese academy of sciences, {2. 100080, china 3Graduate School of the chinese academy of sciences, {2. 100039, china institute of Microbiology Chinese Academy of SciencesBeijing 100080 China Graduate School of the chinese academy of sciencesbeijing 100039 China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

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Structural and microstructural transformations in Bi_2.r_2.a_(1-x)Y_xCu_2._(8+y) system

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chinese Science Bulletin 1997年第3期42卷 192-196页

作者： SONG Chengyu, WEN Shulin and CHE Guangcan1. Shanghai institute of Ceramics, chinese academy of sciences, Shanghai 2.0050,{2. 2. State Key Laboratory of Supercon-ductivity, institute of Physics, chinese academy of sciences, beijing 100080,{2. Shanghai institute of Ceramics Chinese Academy of Sciences Shanghai 200050 China

SINCE the discovery of high-Tc superconductors, experiments have revealed that the phase diagrams of La2.xSrxCuO4 and YBa2.u3O6+x resemble each other. The antiferromagnetic phase is commonly situated in the vicinity o... 详细信息

SINCE the discovery of high-Tc superconductors, experiments have revealed that the phase diagrams of La2.xSrxCuO4 and YBa2./sub>Cu3O6+x resemble each other. The antiferromagnetic phase is commonly situated in the vicinity of the superconductive phase. In Bi-2.12.sys-

关键词： Bi2.r2.a1-xYxCu2.y substitution structural transformation.

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Recent advances in the data analysis method of functional magnetic resonance imaging and its applications in neuroimaging

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Progress in Natural Science:Materials International 2006年第8期16卷 785-795页

作者： TIAN Jie, YANG Lei and HU Jin (institute of Automation, chinese academy of sciences, {2. 100080, china Graduate School of chinese academy of sciences, {2. 100039, china) institute of Automation Chinese Academy of Sciences Beijing 100080 China. Graduate School of chinese academy of sciences Beijing 100039 China

Functional magnetic resonance imaging (fMRI) has opened a new area to explore the human brain. The fMRI can reveal the deep insights of spatial and temporal changes underlying a broad range of brain function, such as motor, vision, memory and emotion, all of which are helpful in the clinical investigation. In this paper, we introduce some recent-developed algorithms for fMRI signal detection such as model-driven method (general linear model, deconvolution model, non-linear model, etc. ) and data-driven method (principle component analysis, independent component analysis, self-organization mapping, clustered constrained non-negative matrix factorization, etc. ). We also propose several important applications of neuroimaging and point out their shortcomings and future perspectives.

关键词： fMR1 data analysis neuroimaging.

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Compression behavior of Zr_(41)Ti_(14)Cu_(12.5)Ni_(10)Be_(2..5) bulk metallic glass up to 2. GPa

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Science china(Physics,Mechanics & Astronomy) 2005年第3期48卷 319-324页

作者： LI Gong 1, 2., ZHAN Zaiji 2., LIU Jing 3 , YUN Jinku 1 , SUN Liling 2., LIU Riping 1 , ZHANG Jun 2.& WANG Wenkui 1, 2.1. Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, china 2. Center for Condensed Matter Physics, institute of Physics,{2., beijing 100080, china 3. BSRF, institute of High Energy Physics,{2., beijing 100039, china Key Laboratory of Metastable Materials Science and Technology Yanshan University Qinhuangdao China Center for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing China BSRF Institute of High Energy Physics Chinese Academy of Sciences Beijing China

The compression of a Zr41Ti14Cu12.5Ni10Be2..5 bulk metallic glass (BMG) is investigated at room temperature up to 2. GPa using in-situ high pressure energy dispersive X-ray diffraction with a synchrotron radiation source. The pressure-induced structural relaxation is exhibited. It is found that below about 8 GPa, the existence of excess free volume contributes to the rapid structural relaxation, which gives rise to the rapid volumetric change, and the structural relaxation results in the structural stiffness under higher pressure.

关键词： bulk metallic glass, high pressure, structural relaxation, synchrotron radiation.

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Megaclasts from the Carboniferous-Permian Rocks in the Langkawi Islands, Malaysia, and Their Implications

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Acta Geologica Sinica(English Edition) 1999年第1期73卷 120-122页

作者： Chen Tingyu institute of geology 2. Baiwanzhuang Road,{2. academy of Geological sciences, beijing 100037,china chinese Acad Geol Sci Inst Geol Beijing 100037 Peoples R China

The Ninth Regional Congress on geology, Mineral andEnergy Resources of Southeast Asia was held in KualaLumpur of Malaysia in August of 1998. After the Con-gress, the author participated in the geological fieldexcursion and investigation in the Langkawi Islands,and noted that the Carboniferous-Permian rocks con-tain megaclasts of metamorphic and pegmatitic rocks,which indicate the existence of pre-Carboniferousmetamorphism and magmatism. The observation resultsare as follows:

关键词： Malaysia and Their Implications Megaclasts from the Carboniferous-Permian Rocks in the Langkawi Islands

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Spectral analysis of quasi-stationary sea surface topography from GRACE mission

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Science china Earth sciences 2005年第11期48卷 2040-2048页

作者： ZHANG Zizhan1,2.& LU Yang1,3 1. institute of Geodesy and Geophysics, chinese academy of sciences, Wuhan 430077,{2. 2. Graduate School of the chinese academy of sciences, beijing 100039,{2. 3. Unite Center for Astro-geodymics Research, chinese academy of sciences, Shanghai 2.0030,{2. institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan China Graduate School of the chinese academy of sciences Beijing China Unite Center for Astro-geodymics Research Chinese Academy of Sciences Shanghai China

During the last two decades satellite altimetry has offered an abundance of meas-urements of the sea surface resulting in the improvement of global mean sea surface height (MSSH) and marine geoid determination. On the other hand, with the launching of new genera-tion gravity satellites, some high accuracy long-wavelength gravity models are available. These breakthroughs give us a great opportunity for new estimation of quasi-stationary sea surface topography (QSST). In this paper, the new gravity model GGM01C derived from GRACE mission is briefly presented, and a new global high precision and high-resolution QSST is determined based on the GGM01C model and the global MSSH. The spectral features of the QSST esti-mated by GGM01C and EGM96 gravity model to degree/order 2.0 are discussed by spectral analysis. As a result, the QSST is mainly composed of long waves, medium waves partially and short waves scarcely, its power spectral structures are different between the zonal direction and the meridional direction, there are great differences between the two models, which maybe ex-plain why the ocean currents derived from the two gravity models by Tapley show different pat-terns.

关键词： quasi-stationary sea surface topography, mean sea surface height, GGM01, fast Fourier transform, spectral analysis

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“Tali Glaciation” on Massif Diancang

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Science china Earth sciences 2007年第11期50卷 1685-1692页

作者： YANG JianQiang1, CUI ZhiJiu2. YI ChaoLu1, SUN JiMin3 & YANG LiRong3 1 institute of Tibetan Plateau Research, chinese academy of sciences, {2. 100085, china 2.College of Environmental sciences, Peking University, {2. 100871, china 3 institute of geology and Geophysics, chinese academy of sciences, beijing 10002., china institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing China College of Environmental sciences Peking University Beijing China institute of geology and Geophysics Chinese Academy of Sciences Beijing China

The term of ''Tali Glaciation'' is nominated from Massif Diancang in Yunnan Province. The confusing process of the term''s being put forward is confirmed through literature checking. Based on several times of field trip, the glacial landforms in this region are studied in detail, and the magnitude of the glaciation was determined. According to the numerical ages from TL, AIMS ^(14)C and OSL dating, the earliest glacial advance on Massif Diancang occurred at 30—40 ka BP, followed by the advances at the last stage of the last glaciation, the late glacial and neoglations in turn, until glaciers vanished at 1.2.1.5 ca. a BP in this region. It can be concluded that glaciers were limited above 3600 m a.s.l., and no glaciers existed on the lower part and adjacent mountains since the last glaciation.

关键词： Massif Diancang,Tali Glaciation,glacial landform,glaciation sequence,numerical dating

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Gasdynamic characteristics of toroidal shock and detonation wave converging

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Science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 739-749页

作者： TENG Honghui1,2.& JIANG Zonglin2.1. Key Laboratory of High Temperature Gas Dynamics, institute of Mechanics, chinese {2. of Sci- ences, beijing 100080, china 2. Graduate University of chinese academy of sciences, beijing 100039, china Key Laboratory of High Temperature Gas Dynamics Institute of Mechanics Chinese Academy of Sciences Beijing China Graduate University of chinese academy of sciences Beijing China

The modified CCW relation is applied to analyzing the shock, detonation wave converging and the role of chemical reactions in the process. Results indicate that the shock wave is strengthened faster than the detonation wave in the converging at the same initial Mach number. Euler equations implemented with a detailed chemical reaction model are solved to simulate toroidal shock and detonation wave converging. Gasdynamic characteristics of the converging are investigated, including wave interaction patterns, observable discrepancies and physical phenomena behind them. By comparing wave diffractions, converging processes and pressure evolutions in the focusing area, the different effects of chemical reactions on diffracting and converging processes are discussed and the analytic conclusion is demonstrated through the observation of numerical simulations.

关键词： converging, CCW relation, shock wave, detonation wave.

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Simulation of aerodynamic performance affected by vortex generators on blunt trailing-edge airfoils

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Science china(Technological sciences) 2010年第1期53卷 1-7页

作者： YANG Ke1,2.ZHANG Lei1,3 & XU JianZhong1,2.1 institute of Engineering Thermophysics,chinese {2. of sciences,beijing 100190,china 2.Key Laboratory of Wind Energy Utilization,chinese {2. of sciences,beijing 100190,china 3 Graduate School of chinese academy of sciences,beijing 100190,china institute of Engineering Thermophysics Chinese Academy of Sciences Beijing China Key Laboratory of Wind Energy Utilization Chinese Academy of Sciences Beijing China Graduate School of chinese academy of sciences Beijing China

An investigation was carried out by numerical simulation on a wind turbine airfoil and a blunt trailing-edge airfoil with and without vortex generators (VGs), and the performance of the airfoils was analyzed. By the simulation of airfoil DU 91-W2.2.0 it was verified that the numerical method and model were credible. Based on this airfoil, a new one with a blunt trailing edge of 6% chord was blended by symmetrically adding thickness, and its characteristics curves were obtained through computing at key angles of attack. Additionally, the pressure distribution on blended airfoil was analyzed by comparing to the airfoil without blend. The interaction of streamwise vortices induced by VGs with trailing vortex or separation vortex was considered, followed by the uncovery of how VGs can suppress the boundary layer separation.

关键词： vortex generators airfoil blunt trailing-edge

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Estimating possible impact of human activity on the warm season temperature in beijing since the industrial era

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chinese Science Bulletin 2010年第16期55卷 1590-1593页

作者： QIN Li1,2.& ZHOU Xin1,2.1 institute of geology and Geophysics,{2. academy of sciences,beijing 10002.,china 2.Graduate University of chinese academy of sciences,beijing 100049,china institute of geology and Geophysics Chinese Academy of Sciences Beijing China Graduate University of chinese academy of sciences Beijing China

Global surface temperature shows an increasing trend over the past 100 *** and natural factors have been invoked to explain this warming, but their relative contribution and the spatial variability remain *** on a 2.50-year temperature record from a stalagmite near beijing, we explore the possible impacts of human activity on the warm season temperature in beijing since the industrial era using the method of singular spectrum analysis (SSA).The results suggest that the warm season temperature in beijing under the natural forcing should have an overall decreasing trend for the past 130 years (1870 to 2.00 AD) with an amplitude of 0.45±0.84°C, while the instrumental record and stalagmite data show an increase of 1.08°*** imply that human activity would have caused an increase in the warm season temperature of beijing for about 1.53±0.84°C compared to its natural trends, from 1870 AD to 2.00 *** results also suggest that anthropogenic forcing began to significantly influence the temperature since 1870 AD, and the effect further intensified since 1915 AD.

关键词：人类活动北京温度工业时代季节温暖估计地表气温

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