检索结果-南通市图书馆

Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure

Science china(Life Sciences) 2006年第2期49卷 97-104页

作者： SHEN Sile1,3, WANG Zhenwei3, SHAN Xiaohui2. WANG Hua3, LI Ling3, LIN Xuyun2. LONG Likun2. WENG Kenan4, LIU Bao2.& ZOU Guangtian1 1. National Laboratory of Superhard Materials, Jilin university, changchun 130012.{2. 2. institute of genetics and cytology, northeast normal university, changchun 130024,{2. 3. College of Life Science, Jilin university, changchun 130012.{2. 4. Guangzhou institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640,{2. National Laboratory of Superhard Materials Jilin University Changchun China College of Life Science Jilin University Changchun China institute of genetics and {2. Northeast Normal University Changchun China Guangzhou institute of Geochemistry Chinese Academy of Sciences Guangzhou China

By using high-pressure treatment, two mutant lines were obtained from agenetically stable japonica rice cultivar Bijing38. Genomic DNA of the mutant lines, together withthe original line (Bijing38), was either undigested or digested by Hpa ⅡIMspⅠ, and then subjectedto molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCRamplification profiles of both markers are apparent in the two mutant lines compared with theoriginal rice cultivar, suggesting that there had been both sequence changes and DNA methylationmodifications in the mutant lines. Southern blot analysis using diverse sequences, including twocellular genes (S2.and S3), a set of retrotransposons (Osr7, Osr36, Tos19 and more), and a MITEtransposon family (mPing and Pong), confirmed the results, and indicated that changes in DNAmethylation pattern, genomic structure, and possible activation of some transposons indeed occurredin the mutant lines. Moreover, these changes are stably maintained through selfed generations and indifferent organs. Thus, our results indicate that it is possible to obtain stable mutants in riceby high pressure treatments, and the molecular basis of the mutants may include both genetic andepigenetic changes. Therefore, high hydrostatic pressure seems a promising approach for plantmutagenesis.

关键词： DNA methylation, genomic changes, high pressure, mutagenesis, rice.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Foreign peptides displayed on the major coat protein of filamentous bacteriophage

引用

Chinese Science Bulletin 1998年第14期43卷 1158-1162页

作者： WANG Li institute of genetics and {2., northeast normal university, changchun 130024, china institute of genetics and {2. Northeast Normal University Changchun China

The genome of filamentous bacteriophage can be engineered to display foreign peptides on the surface of the major coat protein. This display system offers an effective approach to researching the immunological recognition of protein. Studies show that this system has several advantages: 1) the specificity of the immune response; 2. the ability to recruit helper T cells; 3) needs no external adjuvants; 4) the structural mimicry of peptide epitopes. These suggest that this technology could be developed into a simple and inexpensive means to produce new biological reagents and vaccines. The development of this technology and the main property of filamentous bacteriophage are introduced in this paper. Some novel results about foreign peptides display using the main coat protein of filamentous bacteriophage are also summarized.

关键词： filamentous bacteriophage major coat protein epitope.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Function of c-Fos-like and c-Jun-like Proteins on Trichostatin A-induced G2.M Arrest in Physarum polycephalum

引用

Acta Biochimica et Biophysica Sinica 2005年第11期37卷 767-772页

作者： Xiao-Xue LI Jun LU Yan-Mei ZHAO Bai-Qu HUANG institute of genetics and {2.,northeast normal university,changchun 130024,china institute of genetics and {2. Northeast Normal UniversityChangchun 130024 China

The homologs of transcription factors c-Fos and c-Jun have been detected in slime moldPhysarum polycephalum during progression of the synchronous cell *** we demonstrated that c-Fos-like and c-Jun-like proteins participated in G2.M transition by the regulation of the level of Cyclin B1protein in *** study of antibody neutralization revealed that interruption of the functions ofc-Fos-like and c-Jun-like proteins resulted in G2.M transition arrest,implicating their functional roles in cellcycle *** G2.M transition was blocked by histone deacetylase inhibitor trichostatin A,changes inc-Fos- and c-Jun-like protein levels,and hyperacetylation of c-Jun-like protein,were *** datasuggest that in ***,c-Fos- and c-Jun-like proteins may be the key factors in the regulation ofhistone acetylation-related G2.M transition,involving the coordinated expression and hyperacetylation ofthese proteins.

关键词： c-Fos-like protein c-Jun-like protein trichostatin A G2.M transition Physarum polycephalum

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Construction of the Seed-Coat cDNA Microarray and Screening of Differentially Expressed Genes in Barley

引用

Acta Biochimica et Biophysica Sinica 2004年第10期36卷 695-700页

作者： Jin-Song PANG*, Meng-Yuan HE, and Bao LIU Laboratory of Molecular Epigenetics, institute of genetics and cytology, northeast normal university, changchun 130024, china Laboratory of Molecular Epigenetics Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152. that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, to study this question. Six-thousand and forty-eight clones from the wild type Morex testa+pericarp cDNA library were amplified using PCR, and the DNA fragments were spotted on commercial amino-modified glass slide as microarray. The mRNAs from the developing seed coat (8–15 days) of both the mutant and the wild-type barley plants were isolated, and labeled respectively with Cy3-dUTP and Cy5-dUTP when reversely trans- cribed to cDNAs. The labeled cDNAs were used as probes, mixed at the same molar concentration, and hybridized with the DNA fragments on the slide. Seventy clones exhibiting marked differential expression (ratio>4) were identified from the microarray. All the 2. cDNA clones that showed an over-expression in wild type in comparison to the mutant ant13-152.were sequenced. It was found that most of these over- expressing clones were transcription/translation and hordein-associated genes. These results have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants.

关键词： barley seed coat proanthocyanidin cDNA microarray

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Immunolcocalization of actin in intact and DNA-and histone-depleted nuclei and chromosomes of allium cepa

引用

Cell Research 1998年第1期8卷 51-62页

作者： WAN LI HONG MIAO XING (institute of genetics and {2., northeast normal university, changchun 130024, china) institute of genetics and {2. Northeast Normal University China

The presence of actin in eukaryotic nuclei and chromosomes, and especially in higher plant nuclei and chromosomes, has not been well established. We detected actin in meristematic cells of Allium cepa with indirect immunofluorescence technique and observed bright fluorescence in the intact nuclei and chromosomes, indicating that actin is present in the nuclei and chromosomes of the higher plant. We labeled sections of the meristematic cells of A. cepa with immunogold technique, gold particles were found over the whole nuclei and a number of gold particles were concentrated in condensed chromatin and nucleoli, confirming the results of the immunofluoresence observations. We treated the nuclei and chromosomes of *** with DNase I and 2. NaCl and obtained DNA- and histone-depleted nuclei and chromosomes. Indirect immunofluorescence tests showed that the DNA- and histonedepleted nuclei and chromosomes reacted positively with the anti-actin antibodies. These results demonstrate that actin exists not only in intact nuclei and chromosomes,but also in DNA- and histone-depleted nuclei and chrmosomes of the plant. In addition, our immuno-fluorescence tests indicate that tropomyosin is present in the nuclei and chromosomes of A. cepa.

关键词： Actin Allium cepa chromosomes nuclei

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

hELP3 Subunit of the Elongator Complex Regulates the Transcription of HSP70 Gene in Human Cells

引用

Acta Biochimica et Biophysica Sinica 2007年第6期39卷 453-461页

作者： Qiuju HAN, Xiaozhe HOU, Dongmei SU, Lina PAN, Jizhou DUAN, Liguo CUI, Baiqu HUANG, and Jun LU~* institute of genetics and {2., northeast normal university, changchun 130024, china institute of genetics and {2. Northeast Normal UniversityChangchun 130024 China

The human Elongator complex is remarkably similar to its yeast counterpart in several aspects. In a previous study, we analyzed the functions of the human elongation protein 3 (hELP3) subunit of the human Elongator by using an in vivo yeast complementation system. However, direct evidence for hELP3 functions in regulating gene expression in human cells was not obtained. In this study, we used hELP3 antisense oligonucleotide inhibitors to knock down hELP3 gene expression to investigate its function in human 2.3T cells. The results showed that specific reduction of hELP3 mRNA and protein caused a significant suppression of HSP70-2.gene expression, and this was accompanied by histone H3 hypoacetylation and decreased RNA polymerase Ⅱ density at the HSP70-2.gene. Moreover, the data also showed that hELP3 exerted the transcriptional regulatory function directly through its presence on the HSP70-2.gene. Data presented in this report provide further insight and direct evidence of the functions of hELP3 in HSP70-2.gene transcriptional elongation in human cells.

关键词： hELP3 histone acetylation transcription elongation antisense

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

引用

Cell Research 1999年第1期9卷 61-69页

作者： ZENG XIAN LU MING DA JIAO MIAO XING XIAO GUANG WANG SHUI HAO(institute of genetics and {2., northeast normal university, changchun 130024, china)(Department of Biology, changchun normal College,changchun 130032. china) institute of genetics and {2. Northeast Normal University Changchun China Department of Biology Changchun Normal College Changchun China

The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2.M NaCl. SDS-PAGE analyses revealed that the nuclear matrir and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in *** results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic obserwtions further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.

关键词： Chromosome scaffold nuclear matrix Physarum polycephalum tropomyosin

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Transcription Factors Ets2.and Sp1 Act Synergistically with Histone Acetyltrans-ferase p300 in Activating Human Interleukin-12.p40 Promoter

引用

Acta Biochimica et Biophysica Sinica 2006年第3期38卷 194-200页

作者： Hai-Jing SUN~(1,2. Xin XU~1 Xiu-Li WANG~1 Liang WEI~1 Fen LI~1 Jun LU~1* Bai-Qu HUANG~11 institute of genetics and {2.,northeast normal university,changchun 130024,china2.Beijing normal university,Beijing 100875,china institute of genetics and {2. Northeast Normal UniversityChangchun 130024 China Beijing normal university Beijing 100875 China

There has been considerable interest in researching the regulatory mechanisms that control thesynthesis of interleukin （IL）-12.which plays a central role in the differentiation of T-helper-1 *** thisstudy,we performed a series of transient transfection experiments designed to elucidate the functional rela-tionship between the IL-12.promoter-specific transcription factors （Ets2.and Sp1） and histone acetylationmodification in IL-12.regulation mediated by p300 and various histone deacetylases （HDACs）.Results pre-sented in this report demonstrated that the transcription factors Ets2.and Sp1 acted synergistically with p300to activate the human IL-12.*** histone acetyltransferase （HAT） activity of p300 was required forthis synergic effect,because the adenovirus E1A protein inhibited the ***,HDACs re-pressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12.whilep300 was able to rectify *** data indicated that Ets2.and Sp1 worked concertedly and synergisticallywith p300 in the regulation of human IL-12.expression.

关键词： Ets2 Sp1 histone acetyltransferase p300 interleukin-12

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A PSTAIRE CDK-like protein localizes in nuclei and cytoplasm of Physarum polycephalum and functions in the mitosis

引用

Cell Research 2004年第2期14卷 169-175页

作者： Gui Ying LI~(1,2., Miao XING~(1,*), Bo HU~(1,3) ~1College of Life Science, Shenzhen Key Laboratory of Microbial Gene Engineering, Shenzhen university, Shenzhen 518060, china. ~2.epartment of Immunology, School of Basic Medical Sciences, Jilin university, changchun 13002., china ~3institute of genetics and cytology, northeast normal university, changchun 130024, china. College of Life Science Shenzhen Key Laboratory of Microbial Gene Engineering Shenzhen University Shenzhen China Department of Immunology School of Basic Medical Sciences Jilin University Changchun China institute of genetics and {2. Northeast Normal University Changchun China

CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present, among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot, immunoelectron microscopy and antibody treatment with an anti-PSTAIRE monoclonal antibody to explore the subcellular localization and functions of PSTAIRE CDKs in Physarum polycephalum. The results of Western blot and immunoelectron microscopy showed that in P. polycephalum, a PSTAIRE CDK-like protein was 34 kD in molecular weight and located in both nuclei and cytoplasm. In nuclei, the protein was mainly associated with chromosomes and nucleoli. The expression of the PSTAIRE CDK-like protein in both the plasmodia and nuclei showed little fluctuation through the whole cell cycle. When treated with an anti-PSTAIRE monoclonal antibody at early S phase, the cells were arrested in S phase, and the mitotic onset of P. polycephalum was blocked for about 1 h when treated at early G2.phase. Our dat

关键词： PSTAIRE CDK-like protein Physarum polycephalum mitosis immunoelectron microscopy.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

引用

Acta Biochimica et Biophysica Sinica 2005年第7期37卷 440-446页

作者： Li-Hui ZHAO~(1,2. Xue-Qing BA~1 Xiao-Guang WANG~3 Xiao-Juan ZHU~1 Li WANG~1 Xian-Lu ZENG~1 1 institute of genetics and {2.,northeast normal university,changchun 130024,china 2.College of Agronomy,Jilin Agricultural university,changchun 130118,china 3 Department of Biology,changchun normal College,changchun 130032.china institute of genetics and {2. Northeast Normal UniversityChangchun 130024 China College of Agronomy Jilin Agricultural UniversityChangchun 130118 China Department of Biology Changchun Normal CollegeChangchun 130032 China

Brg-or hBrm-associated factor (BAF) complexes,a chromatin-remodeling complex family ofmammalian cells,facilitate transcriptional activity by remodeling nucleosome ***1 is the coresubunit of Brg-associated factor *** the present study,we investigated the spatial relationshipbetween Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptionalactivation in vivo by employing immuno-gold *** data showed that Brg1 was closely co-localizedwith NF1/CTF and RNAP Ⅱ in HeLa ***,the co-immunoprecipitation assay further revealedthat Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated,but not the resting,T *** combined results suggested that BAF complexes can interact with NF1/CTF and RNAPⅡ,and this interaction is closely dependent on the activation of gene transcription.

关键词： BAF complex NF1/CTF RNAP Ⅱ gene transcription

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：