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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, institute of Hydrobiology, chinese academy of sciences, Wuhan 430072. {2. 2. Graduate School of chinese academy of sciences, Beijing 100039, {2.) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of chinese academy of sciences Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

Experimental study of electron capture excitation in collisions between Li-like N^(4+) ions and Na atoms

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chinese Science Bulletin 1997年第5期42卷 368-371页

作者： LIU Zhanwen, ZHOU Sixin, ZHANG Wen, ZHANG Xuezhen GUOXiaohong, WANG Youde, MA Xinwen, YANGZhihu, LIU Huiping, ZHAO Mengchun, YANG Feng, PAN Guangyan and LI Dawan1. institute of Modern Physics, chinese academy of sciences, Lanzhou 730000, {2. 2. institute of Physics, chinese academy of sciences, Beijing 100080, {2. institute of Modern Physics Chinese Academy of Sciences Lamhou China institute of Physia Chinese Academy of Sciences Beijing China

WITH the rapid development of the technique of ionized atoms, the charge states of atoms that can be obtained now are so high that the H-like U91+ ions and naked U92. ions

关键词： single electron capture double electron capture emission cross section excited states.

Novel gaseous transient species:generation and characterization

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Science china chemistry 2007年第2期50卷 145-169页

作者： ZENG XiaoQing1,2.& WANG DianXun11 State Key Laboratory for Structural chemistry of Unstable and Stable Species,institute of chemistry,chinese academy of Sci-ences,Beijing 100080,china 2.Graduate School of chinese academy of sciences,Beijing 100039,china State Key Laboratory for Structural chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing China Graduate School of chinese academy of sciences Beijing China

Due to special properties of transient species, such as short-lived, unstable, reactive, and even explosive, the generation and subsequent characterization is a great challenge for experimental chemists. In our laboratory, systematic researches have been carried out to investigate novel transient species: reactive halogen species (RHS), short-lived free radicals, and metastable pseudohalogen compounds, based on the successive technical improvements on the Hel Photoelectron spectroscopy (PES). In this review, the topic mainly focuses on innovative methods of generating novel transient species, and subsequent geometric and electronic structure characterizations of these species combined with quantum chemical calculations.

关键词： reactive halogen species radicals pseudohalogen compounds Hel Photoelectron Spectroscopy (PES) quantum chemical calculations

Properties and applications of cold supersonic gas jet

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 709-715页

作者： ZHANG Shaofeng1,2.MA Xinwen1,2.LIU Huiping2.LI Bin1,2.& ZHU Xiaolong1,2.1. institute of Modern Physics,chinese academy of sciences,Lanzhou 730000,{2. 2. Graduate University of chinese academy of sciences,Beijing 100049,{2. institute of Modern Physics Chinese Academy of Sciences Lanzhou China Graduate University of chinese academy of sciences Beijing China

By analyzing the formation mechanism of a supersonic gas jet,a set of equations which describe the atomic beam properties were established. The influence of initial temperature,initial pressure,background gas pressure and pumping speed was discussed in detail. A simulation program was developed based on the equations,and the results under different initial conditions were obtained. The results are in good agreement with the experimental data,and suggest that,in order to get much smaller transverse momentum in collision experiments,it is necessary to lower the initial temperature and the initial pressure of the supersonic gas jet,together with increasing the pumping speed. These results are very instructive for construction of a new generation of cold supersonic gas jets.

关键词： cold supersonic gas jet,zone of silence,nozzle,skimmer.

Research progress in the inner surface modification of metal tubes by plasma processing

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Progress in Natural Science:Materials International 2006年第11期16卷 1119-1126页

作者： ZHANG Guling~ 1,2.**, WU Xingfang~1, GU Weichao~2. CHEN Guangliang~2. FENG Wenran~2. NIU Erwu~2. LI Li~2. L Guohua~2. CHEN Huan~2. FAN Songhua~2. LIU Chizi~2.and YANG Size~2.1. School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, china 2. Beijing National Laboratory for Condensed Matter Physics, institute of Physics, {2., Beijing 100080, china) School of Material Science and Engineering University of Science and Technology Beijing Beijing 100083 China Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The recent progress in inner surface hardening of tubes by plasma processing is summerized. Several techniques of inner surface plasma source ion implantation and deposition are introduced, and their advantages and disadvantages are discussed. The basic principles, technical features and new progress of inner surface plasma source ion implantation methods for metal tubes, which were developed in our laboratory, are described in detail. And perspectives of the future technical development for inner surface ion implantation of tubes are presented.

关键词： PSII metal tubes inner surface surface modification.

Adjoint method for the optimum planning of industrial pollutant sources

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Science china Earth sciences 2005年第8期48卷 1270-1279页

作者： LIU Feng1,2. HU Fei2.& ZHU Jiang2.1. College of Environmental sciences, Peking University, Beijing 100871, china 2. LAPC, {2. of Atmospheric Physics, chinese academy of sciences, Beijing 10002., china College of Environmental sciences Peking University Beijing China LAPC Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China

The optimum planning of industrial pollutant sources, which optimizes the economic object without violating environmental constraints, is an important and hard task to be conquered. In this paper, an adjoint method is developed to solve the problem. The penalty function is in-troduced to deal with the environmental inequality constraints, and Lagrange function is con-structed to derive the adjoint equation and the gradient of the object function. In this means, the gradient of the object function can be calculated by solving the adjoint equation, and the infor-mation from the gradient is used to make the object function descend and approach to an optimal solution after some iterations. A two-dimensional, simplified model is used for numerical experi-ments. The theoretical derivations are verified by the results of the experiments. Furthermore, the adjoint method is shown to be of excellent convergence and efficiency, which is adaptive to the fast development of air quality numerical models and super computers.

关键词： optimum planning, industrial pollutant sources, adjoint equation, penalty function, gradient.

Effect of rock mass structure and block size on the slope stability——Physical modeling and discrete element simulation

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Science china(Technological sciences) 2005年第Z1期48卷 1-17页

作者： LI Shihai1, LIAN Zhenzhong1,2.& J. G. Wang3 1. institute of Mechanics, chinese academy of sciences, Beijing {2., china 2. Graduate School, chinese academy of sciences, Beijing 100060, china 3. Tropical Marine Science institute, National University of Singapore, Singapore 1192.0 institute of Mechanics Chinese Academy of Sciences Beijing 100080 China Graduate School Chinese Academy of Sciences Beijing 100060 China Tropical Marine Science institute National University of Singapore Singapore 119260 Singapore

This paper studies the stability of jointed rock slopes by using our improved three-dimensional discrete element methods (DEM) and physical modeling. Results show that the DEM can simulate all failure modes of rock slopes with different joint configurations. The stress in each rock block is not homogeneous and blocks rotate in failure development. Failure modes depend on the configuration of joints. Toppling failure is observed for the slope with straight joints and sliding failure is observed for the slope with staged joints. The DEM results are also compared with those of limit equilibrium method (LEM). Without considering the joints in rock masses, the LEM predicts much higher factor of safety than physical modeling and DEM. The failure mode and factor of safety predicted by the DEM are in good agreement with laboratory tests for any jointed rock slope.

关键词： rock masses, slope stability, DEM simulation, block size effect, joints configuration effect, limit equilibrium method.

Wet-chemical synthesis and characteristics of Au nanoshell

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Science china chemistry 2005年第5期48卷 431-435页

作者： LIU Zhongxin1,2. SONG Hongwei1, YU Lixin1,2. YANG Linmei1,2.& PAN Guohui1,2.1. Key Laboratory of Excited State Physics, Changchun institute of Optics, Fine Mechanics and Physics, chinese academy of sciences, Changchun 130033, {2. 2. Graduate School of the chinese academy of sciences, Beijing 100039, {2. Key Laboratory of Excited State Physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun China Graduate School of the chinese academy of sciences Beijing China

Gold nanoshells were prepared by an easy wet-chemical method, with Ag nanoparticles used as the templates. Transmission electron microscopy (TEM) indicated that the shells were sphere in shape, the size was homogenous, being about 2. nm, and no hard ag-glomerates were observed. The plasma resonance absorption of gold was tuned from visible to near-infrared (NIR) with the increased volume of HAuCl4. The temperature grads (?T) of the gold nanoshell hydrosol under the exposure of an 808-nm optical fiber laser with different power den-sities were measured. The highest ?T was 30℃ (5 W/cm2. irradiation area was 2.cm2.. This kind of gold nanoshell hydrosol is a promising material to be used in biomedicine such as photo-thermal cancer therapy, and its special photothermal convert property will photothermally trigger drug release.

关键词： wet-chemical synthesis, Au, nanoshell, NIR absorption, photothermal conversion.

Boundedness of solutions and existence of invariant tori for generalized pendulum type equation

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chinese Science Bulletin 1997年第20期42卷 1673-1675页

作者： HUANG Hai and YUAN Rong1. institute of Mathematics, chinese academy of sciences, Beijing {2., china 2. Department of Mathematics, Beijing Normal University, Beijing 100875, china institute of Mathematics Chinese Academy of Sciences Beijing China Department of Mathematics Beijing Normal University Beijing China

MOTIVATED by various significant applications to non-Newtonian fluid theory, diffusion offlows in porous media, nonlinear elasticity, and theory of capillary surfaces, several authors(see refs.[1,2. and references cited therein ) have recently studied the existence of periodicsolutions and other properties for the following differential equation:

关键词： boundeduess of solutions invariant tori quasiperlodic solutions Moser’s small twist theorem.