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Identification of glutamate transporters and receptors in mouse testis

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Acta Pharmacologica Sinica 2004年第03期25卷 112-117页

作者： Jia-hua HU,Na YANG2.Ying-hua MA,Jie JIANG,Jin-fu ZHANG3,Jian FEI4,Li-he GUO4 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0031 3{2. of Urology,tongji hospital,tongji university,shanghai 200065,china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences department of Urology Tongji Hospital Tongji University

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.2.) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2., GluR6 and KA2. were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake

Mid-ventricular hypertrophic obstructive cardiomyopathy(MVHOCM) complicated with coronary artery disease:a case report

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Journal of Geriatric Cardiology 2008年第3期5卷 190-192页

作者： Haoming Song Cuimei Zhao Jinfa Jiang Yang Liu Yihan Chen department of Cardiology,tongji {2., tongji university, shanghai, 200065, P.R.china Dept. of Cardiology Tongji Hospital Tongji University 389 Xincun Rd. Shanghai 200065 China

Mid-ventricular hypertrophic obstructive cardiomyopathy (MVHOCM) is a rare type ofcardiomyopathy that can be accompanied by apical *** presented here a case report of MVHOCM with cornary artery *** sixty-four years old man was sent to hospital because ofventricular *** inversion T wave was showed on electrocardiography in the presence of abnormal coronary arteries and normal cardiac *** showed an hourglass appearance of the left ventricle with an aneurysm in the apex and a pressure gradient between the outflow tract of left ventricle and the middle of the left ventricle was revealed by left-heart catheterization.

关键词： hypertrophic obstructive cardiomyopathy coronary artery disease echocardiography

Influence of L-methionine-deprived total parenteral nutrition with 5-fluorouracil on gastric cancer and host metabolism

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World Journal of Gastroenterology 2001年第5期7卷 698-701页

作者： Hong-Bing Xiao~1 Wei-Xin Cao~2.Hao-Ran Yin~2.Yan-Zhen Lin~2.Shi-Hui Ye~1 1 department of Surgery,Affiliated Railway hospital,{2. university,shanghai 2.0072.china2.department of Surgery,Affiliated Ruijin hospital,shanghai Second Medical university,shanghai 2.002.,china department of Surgery Affiliated Railway Hospital Tongji University Shanghai 200072 China. xhawbin@***

AIM: To investigate the influence of L-methionine-deprived total parenteral nutrition with 5-FU on gastric cancer and host metabolism. METHODS: N-methyl-N'-nitro-nitrosoguanidine (MNNG) induced gastric cancer rats were randomly divided into four groups: Met-containing TPN group (n=11), Met-deprived TPN group (n =12., Met-containing TPN+5-FU group (n=11) and Met-deprived TPN+5-FU group (n=12.. Five rats in each group were sacrificed after 7 days of treatment and the samples were taken for examination. The remaining rats in each group were then fed separately with normal diet after the treatment until death, the life span was noted. RESULTS: The tumors were enlarged in Met-containing group and shrank in Met-deprived group markedly after the treatment. The DNA index (DI) of tumor cells and the body weight (BW) of rats had no significant change in the two groups, however, the ratio of tumor cells'S phase was increased. The ratio of G2. phase went up in Met-containing group, but down in Met-deprived group. In the other two groups that 5-FU was added, the BW of rats, and the diameter of tumors, the DI of tumor cells, the S and G2. phase ratio of tumor cells were all decreased, particularly in Met-deprived plus 5-FU group. Pathological examination revealed that the necrotic foci of the tumor tissue increased after Met-deprived TPN treatment, and the nucleoli of tumor cells enlarged. In MetTPN+5-FU group, severe nuclear damage was also found by karyopyknosis and karyorrhexis, meanwhile there was slight degeneration in some liver and kidney cells. The serum free Met and Cysteine decreased markedly (P2..5 days in Met-containing TPN+5-FU group, and 43 days in Met-deprived TPN+5-FU group (P&

关键词： Parenteral Nutrition Animals Antimetabolites, Antineoplastic Body Weight DNA, Neoplasm Fluorouracil Male Methionine Rats Rats, Wistar Research Support, Non-U.S. Gov't S Phase Stomach Neoplasms

Resting state brain activity and functional brain mapping

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Progress in Natural Science:Materials International 2007年第5期17卷 511-519页

作者： Zhao Xiaohu , Wang Peijun and Tang Xiaowei (College of life Sciences in Zhejiang university, Hangzhou 310058, china {2. department of Tong Ji hospital of Tong Ji university, shanghai 200065, china Bio-X Lab, department of Physics, Zhejiang university, Hangzhou 31002., china) [a]College of life Sciences in Zhejiang university Hangzhou China [b]imaging department of Tong Ji {2. of Tong Ji university Shanghai China [c]Bio-X Lab Department of Physics Zhejiang University Hangzhou China

Functional brain imaging studies commonly use either resting or passive task states as their control conditions, and typically identify the activation brain region associated with a specific task by subtracting the resting from the active task conditions. Numerous studies now suggest, however, that the resting state may not reflect true mental "rest" conditions. The mental activity that occurs during "rest" might therefore greatly influence the functional neuroimaging observations that are collected through the usual subtracting analysis strategies. Exploring the ongoing mental processes that occur during resting conditions is thus of particular importance for deciphering functional brain mapping results and obtaining a more comprehensive understanding of human brain functions. In this review article, we will mainly focus on the discussion of the current research background of functional brain mapping at resting state and the physiological significance of the available neuroimaging data.

关键词： resting state brain activity default mode brain networks BOLD deactivation OEF.

Histological Measurem ent of Hum an RetinalThickness

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Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 246-248页

作者： YANG Zhikuan, DU Shuhua department of Ophthalmology, tongji {2., tongji Medical university, Wuhan 430030 department of Ophthalmology Tongji Hospital Tongji Medical University Wuhan 430030

The fresh eye balls from the accidental death adults were placed on low temperature slicer to cut into slices. The fresh sections was subjected to the direct measurement of retinal thickness by using CMIAS multi function real color pathologic imaging analysis system. The results showed that in the total of 6 eye balls measured, the mean retinal thickness was 310.92.34.14 μm. It was concluded that it was simple and had high confidence for the fresh eye ball low temperature frozen sections to directly measure the retinal thickness, which provided a histological method for the measurement of retinal thickness and the reference of retinal thickness of Chinese adults.

关键词： retinal thickness histology

Influence of norcantharidin on proliferation,proliferation-related gene proteins prolifera-ting cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 603-607页

作者： Yue-Zu Fan, Jin-Ye Fu, Ze-Ming Zhao and Cun-Qiu Chen shanghai, china {2. of Surgery, tongji hospital of tongji U- mversity, shanghai 200065, china {2. of Surgery, Pudong People’ s hospital, shanghai 2.12.0 , Chi- na department of Surgery Tongji Hospital of Tongji University 389 Xincun Road Shanghai 200065 China. fanyuezu_shtj@***

BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932.±0.031 vs. 0.318 ±0.02., P2.vs. 0.2.7 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.

关键词： gallbladder neoplasm norcantharidin cell culture immunohistochemistry cell proliferation proliferating cell nuclear antigen, Ki-67

Challenges in the diagnosis and treatment of transsexualism in contemporary china

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上海精神医学 2014年第1期26卷 49-50页

作者： Na LIU Zheng LU shanghai Mental Health Center, shanghai Jiao Tong {2. School of Medicine department of Psychiatry, tongji {2., tongji hospital shanghai Mental Health Center Shanghai Jiao Tong University School of Medicine Shanghai China. shanghai Mental Health Center Shanghai Jiao Tong University School of Medicine Shanghai China Department of Psychiatry Tongji University Tongji Hospital Shanghai China.

According to the International Classification of Diseases10th Edition(ICD-10),[1]transsexualism refers to thecondition where an individual desires to live and to beaccepted as someone of the gender which is oppositetheir biological *** is usually accompanied withdiscomfort or distress about their anatomic *** somecases,individuals with this condition modify their bodyfeatures using laser or plastic surgeries to

关键词：当代中国诊断性欲治疗物理特性整形手术精神障碍生物性

CABYR RNAi plasmid construction and NF-κB signal transduction pathway

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World Journal of Gastroenterology 2010年第39期16卷 4980-4985页

作者： Lin-Xiang Shi,Yao-Ming He,Lin Fang,Hong-Bo Meng,Li-Jun Zheng,department of General Surgery,shanghai Tenth People’s {2.,tongji university,shanghai 2.0072.china department of General Surgery Shanghai Tenth People's Hospital Tongji University Shanghai 200072 China. ajijinwei@***

AIM:To construct the CABYR RNAi plasmid and study its relation with the nuclear factor(NF)-κB signal transduction ***:Human CABYR mRNA sequence was obtained from *** structure of cDNA sequence for the short hairpin RNA was BbsⅠ+sense+loop+ antisense+transcription terminator+KpnⅠ+Bam HⅠ.A CABYR silencing plasmid was constructed and transfected into the human embryo cell line *** real-time polymerase chain reaction was used to analyze CABYR and NF-κB gene ***:The CABYR and NF-κB expressions were detected in 2.3T *** oligonucleotide(5'-GCT-CAGATGTTAGGTAAAG-3')efficiently silenced the expression of *** expression of NF-κB was not significantly affected by silencing CABYR(P=0.743).CONCLUSION:CABYR can be found in the human embryo cell line *** 2.can efficiently silence its target,CABYR,indicating that CABYR is not related with the NF-κB signal transduction pathway.

关键词： CABYR Plasmid Nuclear factor-κB Signal transduction RNAi Cabyrmid 2

Gum mastic increases maspin expression in prostate cancer cells

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Acta Pharmacologica Sinica 2007年第4期28卷 567-572页

作者： Mei-lan HE~(2.3,6),Wei-wen CHEN~(2.6),Peng-ju ZHANG~2.An-li JIANG~2.Wei FAN~4,Hui-qing YUAN~2.Wen-wen LIU~2.Jian-yeZHANG~(2.5)~2.0} of Biochemistry,Medical School,Shandong university,Ji-nan 2.0012.{2. ~3Institute of Cancer Research,Life Science andTechnology School,tongji university shanghai 2.0092.{2. ~4Gastric Intestine department,Affiliated Qi-Lu hospital,Shandong university,Ji-nan 2.0012.{2. department of Biochemistry Medical School Shandong University Ji-nan China Institute of Cancer Research Life Science and Technology School Tongji University Shanghai China Gastric Intestine department Affiliated Qi-Lu Hospital Shandong University Ji-nan China

Aim:To study whether gum mastic,a natural resin,can regulate maspin expres-sion in prostate cancer cells,and further investigate the mechanisms involved inthis regulatory ***:RT-PCR and Western blotting were used todetect maspin expression at the transcriptional and translational *** assay was used to investigate the effect of gum mastic on the maspin *** binding activity of negative androgen-responsive element (ARE) and posi-tive Spl element in the maspin promoter were studied by electrophoretic mobilityshift ***:Gum mastic induced maspin mRNA and protein expression,and the maspin promoter activity was enhanced with gum mastic ***,gum mastic inhibited the ARE binding activity and increased the Sp 1 bindingactivity in the maspin ***:Gum mastic enhances maspin pro-moter activity by suppressing ARE binding activity and enhancing Spl bindingactivity,and the increased activity in the maspin promoter finally leads to the up-regulation of both its mRNA and protein levels.

关键词： gum mastic maspin expression ARE binding activity Spl binding activity