检索结果-南通市图书馆

The susceptibility of ventricular arrhythmia to aconitine in conscious Lyon hypertensive rats

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第2期28卷 211-215页

作者： Min LI~(2.3) Jin WANG~2.He-hui XIE~2.Fu-ming SHEN~2.Ding-feng SU~(2.4)2.department of Pharmacology,Second Military.Medical university,{2. 2.0433,China 3 department of Pediatrics,{2. Tenth People's hospital,tongji university,{2. 2.0072.China department of Pharmacology Second Military Medical University Shanghai China department of Pediatrics Shanghai Tenth People's Hospital Tongji University Shanghai China

Aim:The present work was designed to investigate the relationship betweenhemodynamic parameters and the susceptibility of ventricular arrhythmia toaconitine in conscious Lyon hypertensive rats(LH).Methods:Male LH and Lyonlow blood pressure rats(LL)were *** the determination of baroreflexsensitivity(BRS),ventricular arrhythmia was induced by aconitine infusion inconscious *** pressure(BP)was recorded during the period of ***:Compared with the LL rats,the LH rats possessed significantly higherBP,blood pressure variability and lower *** threshold of aconitine requiredfor ventricular fibrillation and cardiac arrest in the LH rats were significantly lowerthan those in the LL *** was found that all the hemodynamic parametersstudied were not correlated with the threshold of aconitine required for arrhythmia,with the exception of BRS,which was positively related to the threshold ofaconitine required for ventricular premature ***:The LH rats pos-sessed greater susceptibility to aconitine-induced ventricular arrhythmias whencompared to the LL *** greater susceptibility could not be attributed to anyone of the hemodynamic parameters alone studied in the LH *** is proposedthat various hypertension-associated abnormalities,including the abnormalhemodynamics,may co-contribute to this vulnerability to ventricular arrhythmias.

关键词： arrhythmia aconitine baroreflex sensitivity hypertension Lyon hypertensive rats

Identification of glutamate transporters and receptors in mouse testis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第3期25卷 112-117页

作者： Jia-hua HU,Na YANG2.Ying-hua MA,Jie JIANG,Jin-fu ZHANG3,Jian FEI4,Li-he GUO4 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0031 3{2. of Urology,tongji hospital,tongji university,shanghai 2.0065,China 中科院上海生命科学院 Laboratory of Molecular Cell Biology CAS 上海 200031 department of Urology Tongji Hospital Tongji University 上海 200065

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.2.) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2., GluR6 and KA2. were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake

Influence of L-methionine-deprived total parenteral nutrition with 5-fluorouracil on gastric cancer and host metabolism

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2001年第5期7卷 698-701页

作者： Hong-Bing Xiao~1 Wei-Xin Cao~2.Hao-Ran Yin~2.Yan-Zhen Lin~2.Shi-Hui Ye~1 1 department of Surgery,Affiliated Railway hospital,{2. university,shanghai 2.0072.China2.department of Surgery,Affiliated Ruijin hospital,shanghai Second Medical university,shanghai 2.002.,China department of Surgery Affiliated Railway Hospital Tongji University Shanghai 200072 China. xhawbin@***

AIM: To investigate the influence of L-methionine-deprived total parenteral nutrition with 5-FU on gastric cancer and host metabolism. METHODS: N-methyl-N'-nitro-nitrosoguanidine (MNNG) induced gastric cancer rats were randomly divided into four groups: Met-containing TPN group (n=11), Met-deprived TPN group (n =12., Met-containing TPN+5-FU group (n=11) and Met-deprived TPN+5-FU group (n=12.. Five rats in each group were sacrificed after 7 days of treatment and the samples were taken for examination. The remaining rats in each group were then fed separately with normal diet after the treatment until death, the life span was noted. RESULTS: The tumors were enlarged in Met-containing group and shrank in Met-deprived group markedly after the treatment. The DNA index (DI) of tumor cells and the body weight (BW) of rats had no significant change in the two groups, however, the ratio of tumor cells'S phase was increased. The ratio of G2. phase went up in Met-containing group, but down in Met-deprived group. In the other two groups that 5-FU was added, the BW of rats, and the diameter of tumors, the DI of tumor cells, the S and G2. phase ratio of tumor cells were all decreased, particularly in Met-deprived plus 5-FU group. Pathological examination revealed that the necrotic foci of the tumor tissue increased after Met-deprived TPN treatment, and the nucleoli of tumor cells enlarged. In MetTPN+5-FU group, severe nuclear damage was also found by karyopyknosis and karyorrhexis, meanwhile there was slight degeneration in some liver and kidney cells. The serum free Met and Cysteine decreased markedly (P2..5 days in Met-containing TPN+5-FU group, and 43 days in Met-deprived TPN+5-FU group (P&

关键词： Parenteral Nutrition Animals Antimetabolites, Antineoplastic Body Weight DNA, Neoplasm Fluorouracil Male Methionine Rats Rats, Wistar Research Support, Non-U.S. Gov't S Phase Stomach Neoplasms

Establishment of a Screening System for Selection of siRNA Target Sites Directed against Hepatitis B Virus Surface Gene

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第5期37卷 310-316页

作者： Xiu-Min ZHOU~(1.3) Ju-Sheng LIN~1 Yi SHI~3 De-An TIAN~2.Huan-Jun HUANG~2.He-Jun ZHOU~1 You-Xin JIN~3 1 Institute of Liver Diseases. tongji hospital, tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, China: 2.department of Gastroenterology, tongji hospital, tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, China 3 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, shanghai 2.0031, China Institute of Liver Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China department of Gastroenterology Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China

To study the inhibitory effects of plasmid-derived small interfering RNA(siRNA)andsynthetic siRNA on the expression of the hepatitis B virus surface(HBs)gene,three plasmid-derived siRNAsand one synthetic siRNA that complement the coding region of the HBs gene were *** HBsexpression plasmid pHBs-EGFP was also *** cells were co-transfected with pHBs-EGFPand the above *** HBs mRNA quantities were measured by reverse-transcription PCR,and thelevel of HBs-EGFP fusion protein was quantified by fluorescent *** concentrations of thehepatitis B virus surface antigen(HBsAg)derived from the culture supernatant of transfected HepG2.2.15cells were measured by an enzyme-linked immunosorbent assay(ELISA)*** results showed that thethree plasmid-derived siRNAs and the synthetic siRNA can effectively reduce the quantities of HBs mRNAand *** plasmid-derived siRNA psiRNA1 was found to be the most effective inhibitor of *** can inhibit HBs-EGFP expression by 63.3% and suppress HBs mRNA by 75.6%.To furthersubstantiate the above observations,psiRNA1 was transfected into HepG2.2.15 cells(an HBV secreting cellline).The transfections resulted in almost complete blockage of HBsAg production,whereas control vector-transfected cells secreted high levels of HBsAg 7 days *** conclusion,our data suggeststhat RNA interference(RNAi)is an efficient approach for reducing the level of HBs transcripts and proteinsand for suppressing HBsAg production.

关键词： RNA interference(RNAi) small interference RNA(siRNA) hepatitis B virus surface gene(HBs gene)

Histological Measurem ent of Hum an RetinalThickness

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 246-248页

作者： YANG Zhikuan, DU Shuhua department of Ophthalmology, tongji{2., tongji Medical university, Wuhan 430030 department of Ophthalmology Tongji Hospital Tongji Medical University Wuhan

The fresh eye balls from the accidental death adults were placed on low temperature slicer to cut into slices. The fresh sections was subjected to the direct measurement of retinal thickness by using CMIAS multi function real color pathologic imaging analysis system. The results showed that in the total of 6 eye balls measured, the mean retinal thickness was 310.92.34.14 μm. It was concluded that it was simple and had high confidence for the fresh eye ball low temperature frozen sections to directly measure the retinal thickness, which provided a histological method for the measurement of retinal thickness and the reference of retinal thickness of Chinese adults.

关键词： retinal thickness histology

Gum mastic increases maspin expression in prostate cancer cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第4期28卷 567-572页

作者： Mei-lan HE~(2.3,6),Wei-wen CHEN~(2.6),Peng-ju ZHANG~2.An-li JIANG~2.Wei FAN~4,Hui-qing YUAN~2.Wen-wen LIU~2.Jian-yeZHANG~(2.5)~2.0} of Biochemistry,Medical School,Shandong university,Ji-nan 2.0012.China ~3Institute of Cancer Research,Life Science andTechnology School,{2. university shanghai 2.0092.China ~4Gastric Intestine department,Affiliated Qi-Lu hospital,Shandong university,Ji-nan 2.0012.China department of Biochemistry Medical School Shandong University Ji-nan China Institute of Cancer Research Life Science and Technology School Tongji University Shanghai China Gastric Intestine department Affiliated Qi-Lu Hospital Shandong University Ji-nan China

Aim:To study whether gum mastic,a natural resin,can regulate maspin expres-sion in prostate cancer cells,and further investigate the mechanisms involved inthis regulatory ***:RT-PCR and Western blotting were used todetect maspin expression at the transcriptional and translational *** assay was used to investigate the effect of gum mastic on the maspin *** binding activity of negative androgen-responsive element (ARE) and posi-tive Spl element in the maspin promoter were studied by electrophoretic mobilityshift ***:Gum mastic induced maspin mRNA and protein expression,and the maspin promoter activity was enhanced with gum mastic ***,gum mastic inhibited the ARE binding activity and increased the Sp 1 bindingactivity in the maspin ***:Gum mastic enhances maspin pro-moter activity by suppressing ARE binding activity and enhancing Spl bindingactivity,and the increased activity in the maspin promoter finally leads to the up-regulation of both its mRNA and protein levels.

关键词： gum mastic maspin expression ARE binding activity Spl binding activity

Challenges in the diagnosis and treatment of transsexualism in contemporary China

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

上海精神医学 2014年第1期26卷 49-50页

作者： Na LIU Zheng LU shanghai Mental Health Center, shanghai Jiao Tong{2. School of Medicine department of Psychiatry, tongji{2., tongji hospital shanghai Mental Health Center Shanghai Jiao Tong University School of Medicine Shanghai China. shanghai Mental Health Center Shanghai Jiao Tong University School of Medicine Shanghai China Department of Psychiatry Tongji University Tongji Hospital Shanghai China.

According to the International Classification of Diseases10th Edition(ICD-10),[1]transsexualism refers to thecondition where an individual desires to live and to beaccepted as someone of the gender which is oppositetheir biological *** is usually accompanied withdiscomfort or distress about their anatomic *** somecases,individuals with this condition modify their bodyfeatures using laser or plastic surgeries to

关键词：当代中国诊断性欲治疗物理特性整形手术精神障碍生物性

Mid-ventricular hypertrophic obstructive cardiomyopathy(MVHOCM) complicated with coronary artery disease:a case report

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2008年第3期5卷 190-192页

作者： Haoming Song Cuimei Zhao Jinfa Jiang Yang Liu Yihan Chen department of Cardiology,tongji{2., tongji university, shanghai, 2.0065, P.R.China Dept. of Cardiology Tongji Hospital Tongji University 389 Xincun Rd. Shanghai 200065 China

Mid-ventricular hypertrophic obstructive cardiomyopathy (MVHOCM) is a rare type ofcardiomyopathy that can be accompanied by apical *** presented here a case report of MVHOCM with cornary artery *** sixty-four years old man was sent to hospital because ofventricular *** inversion T wave was showed on electrocardiography in the presence of abnormal coronary arteries and normal cardiac *** showed an hourglass appearance of the left ventricle with an aneurysm in the apex and a pressure gradient between the outflow tract of left ventricle and the middle of the left ventricle was revealed by left-heart catheterization.

关键词： hypertrophic obstructive cardiomyopathy coronary artery disease echocardiography

Stimulus-responsive polymeric nanoparticles for biomedical applications

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2010年第3期53卷 447-457页

作者： LI YongYong1,2.DONG HaiQing1,2.WANG Kang1,SHI DongLu 2.ZHANG XianZheng1 & ZHUO RenXi1 1Key Laboratory of Biomedical Polymers,Ministry of Education department of Chemistry,Wuhan university,Wuhan 430072.China 2.nstitute for Advanced Materials and Nano Biomedicine(iNANO),{2. university,shanghai 2.0092.China Key Laboratory of Biomedical Polymers Ministry of Education department of Chemistry Wuhan University Wuhan China Institute for Advanced Materials and Nano Biomedicine (iNANO) Tongji University Shanghai China

Polymeric nanoparticles with unique properties are regarded as the most promising materials for biomedical applications including drug delivery and in vitro/in vivo *** them,stimulus-responsive polymeric nanoparticles,usually termed as "intelligent" nanoparticles,could undergo structure,shape,and property changes after being exposed to external signals including pH,temperature,magnetic field,and light,which could be used to modulate the macroscopical behavior of the *** paper reviews the recent progress in stimulus-responsive nanoparticles used for drug delivery and in vitro/in vivo imaging,with an emphasis on double/multiple stimulus-responsive systems and their biomedical applications.

关键词： polymer stimulus-responsive nanoparticles drug carrier cellular imaging