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Identification of glutamate transporters and receptors in mouse testis

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Acta Pharmacologica Sinica 2004年第03期25卷 112-117页

作者： Jia-hua HU,Na YANG2.Ying-hua MA,Jie JIANG,Jin-fu ZHANG3.Jian FEI4,Li-he GUO4 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.003. 3.2} of Urology,{3. {2.,{3. university,Shanghai 200065,china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences department of Urology Tongji Hospital Tongji University

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.2.) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2., GluR6 and KA2. were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake

Inhibition of ethanol-induced toxicity by tanshinone ⅡA in PC12.cells

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Acta Pharmacologica Sinica 2006年第6期27卷 659-664页

作者： Xian-fang MENG~2.Xiao-jing ZOU~3.Bin PENG~2.Jing SHI~2.Xin-min GUAN~2.Chun ZHANG~(4,5)2.department of Neurobiology,tongji Medical College,Huazhong {2. of Science and Technology,Wuhan 43.03.,china 3.department of Anesthesiology,4 department of Nephrology,Union hospital,tongji Medical College,Huazhong {2. of Science and Technology,Wuhan 43.02..china department of Neurobiology Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To observe the effects of tanshinone IIA(Tan IIA)on the neurotoxicityinduced by ethanol in PC 12.cells and to explore its protective ***:PC 12.ell survival was measured by MTT *** formation of reactive oxygenspecies(ROS)and lactate dehydrogenase(LDH)release were detected by 2.,7′-dichlorofluorescin(DCF)fluorescence and calorimetric method,*** of cell apoptosis was monitored by flow *** expression ofp53.was detected by immuno-fluorescence and flow ***:Ethanolsignificantly impaired the survival of PC12.cells as demonstrated by MTT *** also induced significant ROS formation and increased LDH ***-incubation with Tan IIA in the culture medium significantly reversed these *** caused cell apoptosis and the upregulation of p53.*** anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by thedownregulation of pro-apoptotic p53.protein ***:Tan IIAcan protect neurons from apoptosis and might serve as a potential therapeuticdrug for neurological disorders induced by ethanol.

关键词： apoptosis ethanol PC12.cells p53 tanshinone ⅡA

Effectsof the NovelGH Secretogogue, Hexarelin on GH Se-cretion and PhosphatidylinositolHydrolysis by Hum an Pitu-itary Som atotrophinom as in CellCulture

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Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 233-236页

作者： LIU Qin1 , LEI Ting 2. LIU Kui 2. BAI Xiangjun 2. Eric Adams 3.1 {3. of Pediatrics, Xiehe hospital, {2. Medical university, Wuhan 43.02. 2.{3. of Neurosurgery, {2. hospital, {2. Medical university, Wuhan 43.03. department of Pediatrics Xiehe Hospital Tongji Medical University Wuhan 430022

The effects of the novel GH releasing hexapeptide, Hexarelin, on cultured human pituitary somatotrophinomas were investigated. Hexarelin (0.01 - 100 nmol / L) dose dependently stimulated GH secretion up to 4.6 fold. Maximal effects occurred with 10 nmol / L. These effects were very similar to those observed with GHRP 6. The effects of Hexarelin were reduced by phloretin, an inhibitor of protein kinase C (PKC). The rate of phosphatidylinositol (PI) hydrolysis was markedly increased by Hexarelin in a dose dependent manner. These results demonstrated that Hexarelin could directly stimulate GH secretion by human pituitary somatotrophs in a PKC dependent manner, probably via activation of the PI transduction system.

关键词： Hexarelin somatotrophinomas phosphatidylinositol growth hormone pituitary

NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes

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Acta Pharmacologica Sinica 2004年第6期25卷 12-18页

作者： Bo HU, Sheng-gang SUN2. E-tang TONGNeurology department, Union hospital, {2. College, Huazhong university of Science and Technology, Wuhan 43.02., china Neurology department Union Hospital Tongji College Huazhong University of Science and Technology Wuhan 430022 China

AIM: To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the roleof N-methyl-D-aspartate (NMDA) and alpha-amino-3.hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) re-ceptors in the procedure. METHODS: The fluorescence of calcium was measured by Fura-2.AM (F3.5/F3.0).RESULTS: L-Glutamate induced [Ca2.]i increase in most of the cells in concentration- and time-dependent *** 50 mmol/L induced the fluorescence increase by almost three to four times, while the effect of AMPA 50mmol/L was just half of that of D-(-)-2.amino-5-phosphonopentanoic acid (D-AP-5; a selective antagonist of theNMDA receptor). 6-Cyano-7-nitroquinoxaline-2.3.dione (CNQX, a selective antagonist of the AMPA receptor)abolished the effects of NMDA and AMPA, respectively. D-AP-5 and CNQX simultaneously or respectively attenu-ated the effect of L-glutamate at different degrees, but could not abolish it entirely. CONCLUSION: Glutamatemodulated intracellular Ca2. of pure cultured rat astrocytes through different pathways. The activation of NMDAand AMPA receptors took part in the complex mechanisms.

关键词： glutamate astrocytes NMDA receptors AMPA receptors calcium

TLR4 signaling induced TLR2.expression in the process of mimic cerebral ischemia/reperfusion in vitro

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中国科学：生命科学英文版 2010年第2期53卷 223-228页

作者： SUN Peng1,ZHANG Qing2.HAN JiYuan1,TIAN Yuan3.& ZHANG JingHui3.1department of Emergency,Union hospital,{2. Medical College,Huazhong university of Science and Technology,Wuhan 43.02.,china 2.0} of Anesthesia,Union hospital,{2. Medical College,Huazhong university of Science and Technology,Wuhan 43.02.,china 3.1} of General Surgery,Union hospital,{2. Medical College,Huazhong university of Science and Technology,Wuhan 43.02.,china 1. department of Emergency Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China2. Department of Anesthesia Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China3. Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Both TLR4 and TLR2.participated in the mediation of the inflammatory injury in the process of partial cerebral ischemia/***,it still remains unclear whether a crosstalk exists between TLR2.and TLR4 in ischemic cerebral *** the present study,we investigated the effect of TLR4 signaling on TLR2.expression during mimic cerebral I/R in ***-2.cells were cultured and treated with ischemia/reperfusion,then transfected with the plasmid pEGFP-H1/TLR4-siRNA,the plasmid pEGFP-H1/control sequence-siRNA and the blank plasmid,***,the expression of TLR2.and TLR4 mRNA and protein,NF-κB p65 mRNA and supernatant TNF-α level were significantly higher in ischemia/reperfusion treated cells than those lack of ischemia/reperfusion treatment,and as compared with those in ischemia/reperfusion treated cells without transfection,no significant differences about the above mentioned gene and protein expression were found in the blank plasmid tranfected cells and the plasmid pEGFP-H1/control sequence-siRNA transfected cells respectively,while the expression levels in the plasmid pEGFP-H1/TLR4-siRNA transfected cells were significantly ***,in order to determine the effects of pyrrolidinediethyldithiocarbamate (PDTC),an NF-κB inhibitor,on the TLR4-induced TLR2.expression in BV-2.cells treated with ischemia/reperfusion,it was found that TLR4 and TLR2.mRNA expressions in PDTC pretreated cells were significantly lower in comparison with normal saline pretreated cells and non-pretreated *** data suggested that TLR2.activation,signaled by TLR4 and regulated by NF-κB,might be directly involved play an important role in ischemia/reperfusion induced brain damage.

关键词： Toll-like receptor 4 Toll-like receptor 2 cerebral ischemia/reperfusion microglia

Mid-ventricular hypertrophic obstructive cardiomyopathy(MVHOCM) complicated with coronary artery disease:a case report

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Journal of Geriatric Cardiology 2008年第3期5卷 190-192页

作者： Haoming Song Cuimei Zhao Jinfa Jiang Yang Liu Yihan Chen department of Cardiology,tongji {2., tongji university, Shanghai, 200065, P.R.china Dept. of Cardiology Tongji Hospital Tongji University 389 Xincun Rd. Shanghai 200065 China

Mid-ventricular hypertrophic obstructive cardiomyopathy (MVHOCM) is a rare type ofcardiomyopathy that can be accompanied by apical *** presented here a case report of MVHOCM with cornary artery *** sixty-four years old man was sent to hospital because ofventricular *** inversion T wave was showed on electrocardiography in the presence of abnormal coronary arteries and normal cardiac *** showed an hourglass appearance of the left ventricle with an aneurysm in the apex and a pressure gradient between the outflow tract of left ventricle and the middle of the left ventricle was revealed by left-heart catheterization.

关键词： hypertrophic obstructive cardiomyopathy coronary artery disease echocardiography

Histological Measurem ent of Hum an RetinalThickness

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Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 246-248页

作者： YANG Zhikuan, DU Shuhua department of Ophthalmology, tongji {2., tongji Medical university, Wuhan 43.03. department of Ophthalmology Tongji Hospital Tongji Medical University Wuhan 430030

The fresh eye balls from the accidental death adults were placed on low temperature slicer to cut into slices. The fresh sections was subjected to the direct measurement of retinal thickness by using CMIAS multi function real color pathologic imaging analysis system. The results showed that in the total of 6 eye balls measured, the mean retinal thickness was 3.0.92.3..14 μm. It was concluded that it was simple and had high confidence for the fresh eye ball low temperature frozen sections to directly measure the retinal thickness, which provided a histological method for the measurement of retinal thickness and the reference of retinal thickness of Chinese adults.

关键词： retinal thickness histology

Effect of Astragalus on the Endothelin in Rats with AcuteLung Injury

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Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 206-208,214页

作者： LIU Xiaoqing , Huang Cuiping department of Respiratory Diseases, tongji {2., tongji Medical university, Wuhan 43.03. department of Respiratory Diseases Tongji Hospital Tongji Medical University Wuhan 430030

The effect of astragalus on the endothelin in serum and lung of the rats with acute lung injury was studied. The results demonstrated that the concentration of endothelin in the lung of the rats in therapy group was lower than that of the injured rats (64.3.±5.3. ng/L vs 103.3.0}4.99 ng/L, P 3. ng/L vs 113.3. ng/L, P 2. serum SOD, lung coefficient, ratio of lung wet weight/dry weight in two groups were also significantly different ( P <0.01) respectively, and the lung pathological injury in the treatment group were less than that of injury group. So it is concluded that astragalus could inhibit the increase of serum and lung endothelin, thereby playing a protective role in the rats with acute lung injury.

关键词： endothelin astragalus acute lung injury

A template of rat brain based on fMRI T2. imaging

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Progress in Natural Science:Materials International 2005年第6期15卷 502-506页

作者： HU Zhenghui1, WU Yigen2. WANG Xiaochuan3. WANG Jianzhi3. CHEN Feiyan1 and TANG Xiaowei1*(1. department of Physics and Bio-X Laboratory, Zhejiang university, Hangzhou 3.002.,{2. 2. Institute of High Energy Physics and Key Laboratory of Nuclear Analysis Techniques, Chinese Academy of Sciences,Beijing 10003.,{2. 3. department of Pathophysiology, tongji Medical College, Huazhong university of Science and Technology, Wuhan 43.03.,{2.) department of Physics and Bio-X Laboratory Zhejiang University Hangzhou 310027 China Institute of High Energy Physics and Key Laboratory of Nuclear Analysis Techniques Chinese Academy of Sciences Beijing 100039 China department of Pathophysiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The development of functional magnetic resonance imaging (fMRI) technology has made it possible to carry out functional brain imaging experiments in small animals. Usually, group data is required to form the assessment of population, which can not only increase the sensitivity of the overall experiment, but also allow the generalization of the conclusion to the whole population. In order to average the signals of functional brain images from different subjects, it is necessary to put all the mapping images into the same standard space (template image). However, up to now, most animal brain templates remain unavailable and it must be done by ourselves. In this study, a template image based on the brains of eight male Wistar rats is obtained, and it is successfully used in our present Alzheimer disease (AD)-like rat model studies as template for spatially normalizing images to the same stereotaxical space. The fMRI results processed with statistical parametric mapping (SPM99) software are in agreement with the results from immunohistochemical experiment, which proves that this method is universally applicable to the pathologic models of other small animals and to human brain lesion studies.

关键词： fMRI animal model atlas template normalization T*2.