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A novel endorectal RF coil for high resolution MRI and spectroscopy of the prostate

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Progress in Natural Science:Materials International 2005年第5期15卷 472-476页

作者： ZHANG Hongjie 1,2. SONG Xiaoyu1, WANG Xiaoying3. XU Pingfang1 and BAO Shanglian1*(1. Beijing Key Laboratory of Medical Physics & Engineering, Peking university, Beijing 100871, china 2. Navy General {2., Beijing 10003., china 3. Peking university First {2., Beijing 10003., china) Beijing Key Laboratory of Medical Physics & Engineering Peking University Beijing 100871 China Navy General hospital Beijing 100037 China Peking university First hospital Beijing 100034 China

The diagnosis of prostate diseases by magnetic resonance imaging (MRI) in vivo is sometimes difficult for the lower signal to noise ratio (SNR). To increase the SNR of the prostate image, we designed a RF coil that can be inserted into rectum and was named endorectal coil. The properties of RF coil were evaluated using a network analyzer. Moreover the images and spectroscopy of a special phantom were acquired and the results were compared to those of the commercial TORSO coil (G.E. Medical Systems, USA). Our coil gave a significantly higher SNR at the region of interest (ROI). The achieved high local SNR and resulting high spatial resolution would add more anatomic and biochemical information to the diagnosis of prostate diseases.

关键词： MRI RF coil endorectal coil.

Effectsof the NovelGH Secretogogue, Hexarelin on GH Se-cretion and PhosphatidylinositolHydrolysis by Hum an Pitu-itary Som atotrophinom as in CellCulture

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Journal of Huazhong university of Science and Technology(Medical Sciences) 1999年第3期19卷 233-236页

作者： LIU Qin1 , LEI Ting 2. LIU Kui 2. BAI Xiangjun 2. Eric Adams 3.1 Department of Pediatrics, Xiehe {3., tongji Medical {2., Wuhan 43.02. 2.Department of Neurosurgery, tongji {3., tongji Medical {2., Wuhan 43.03. Department of Pediatrics Xiehe Hospital Tongji Medical University Wuhan 430022

The effects of the novel GH releasing hexapeptide, Hexarelin, on cultured human pituitary somatotrophinomas were investigated. Hexarelin (0.01 - 100 nmol / L) dose dependently stimulated GH secretion up to 4.6 fold. Maximal effects occurred with 10 nmol / L. These effects were very similar to those observed with GHRP 6. The effects of Hexarelin were reduced by phloretin, an inhibitor of protein kinase C (PKC). The rate of phosphatidylinositol (PI) hydrolysis was markedly increased by Hexarelin in a dose dependent manner. These results demonstrated that Hexarelin could directly stimulate GH secretion by human pituitary somatotrophs in a PKC dependent manner, probably via activation of the PI transduction system.

关键词： Hexarelin somatotrophinomas phosphatidylinositol growth hormone pituitary

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Inhibition of ethanol-induced toxicity by tanshinone ⅡA in PC12.cells

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Acta Pharmacologica Sinica 2006年第6期27卷 659-664页

作者： Xian-fang MENG~2.Xiao-jing ZOU~3.Bin PENG~2.Jing SHI~2.Xin-min GUAN~2.Chun ZHANG~(4,5)2.Department of Neurobiology,tongji Medical College,Huazhong university of Science and Technology,Wuhan 43.03.,{2. 3.Department of Anesthesiology,4 Department of Nephrology,Union hospital,tongji Medical College,Huazhong university of Science and Technology,Wuhan 43.02..{2. Department of Neurobiology Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To observe the effects of tanshinone IIA(Tan IIA)on the neurotoxicityinduced by ethanol in PC 12.cells and to explore its protective ***:PC 12.ell survival was measured by MTT *** formation of reactive oxygenspecies(ROS)and lactate dehydrogenase(LDH)release were detected by 2.,7′-dichlorofluorescin(DCF)fluorescence and calorimetric method,*** of cell apoptosis was monitored by flow *** expression ofp53.was detected by immuno-fluorescence and flow ***:Ethanolsignificantly impaired the survival of PC12.cells as demonstrated by MTT *** also induced significant ROS formation and increased LDH ***-incubation with Tan IIA in the culture medium significantly reversed these *** caused cell apoptosis and the upregulation of p53.*** anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by thedownregulation of pro-apoptotic p53.protein ***:Tan IIAcan protect neurons from apoptosis and might serve as a potential therapeuticdrug for neurological disorders induced by ethanol.

关键词： apoptosis ethanol PC12.cells p53 tanshinone ⅡA

Chinese expert consensus on the clinical application of drugcoated balloon(2.(nd) Edition)

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Journal of Geriatric Cardiology 2024年第2期21卷 135-152页

作者： The Expert Writing Committee of the Chinese Expert Consensus on Clinical Applications of Drug-Coated Balloon(2.(nd)Edition) Jun-Bo GE Yun-Dai CHEN 不详 Zhongshan hospital Fudan University Chinese PLA General hospital

Percutaneous coronary interventions have progressed through the era of plain balloon dilation, bare-metal stent insertion to drug-eluting stent treatment, which has significantly reduced the acute occlusion and restenosis rates of target vessels and improved patient prognosis, making drug-eluting stents the mainstream interventional treatment for coronary artery disease. In recent years, drug-coated balloons(DCBs) have become a new treatment strategy for coronary artery disease, and the drugs used in the coating and the coating technology have progressed in the past years. Without permanent implant, a DCB delivers antiproliferative drugs rapidly and uniformly into the vessel wall via the excipient during a single balloon dilation. Many evidence suggests that DCB angioplasty is an effective measure for dealing with in-stent restenosis and de novo lesions in small coronary *** more clinical studies are published, new evidence is emerging for the use of DCB angioplasty in a wide range of coronary diseases, and the indications are expanding internationally. Based on the latest research from china and elsewhere, the Expert Writing Committee of the Chinese Expert Consensus on Clinical Applications of Drug-Coated Balloon has updated the previous DCB consensus after evidence-based discussions and meetings in terms of adequate preparation of in-stent restenosis lesions, expansion of the indications for coronary de novo lesions, and precise guidance of DCB treatment by intravascular imaging and functional evaluation.

关键词： balloon drugs dealing

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Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402.involving G2.phase arrest

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Acta Pharmacologica Sinica 2004年第12期25卷 132-138页

作者： Hong-ying CHEN2. Cong-yi ZHENG2. Guo-lin ZOU2. Da-xing XIE3. Jian-ping GONG3. 2.ollege of Life Sciences, Wuhan university, Wuhan 43.072.3.ancer Research Institute, tongji {2., tongjiMedical College, Huazhong university of Science and Technology, Wuhan 43.072. china College of Life Sciences Wuhan University Wuhan 430072 China

AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402.. METHODS: Growth inhibition by PEP was analyzed using 3. 4,5-Dimethylthiazol-2.yl-2.5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 3.2.8 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 μmol/Lfor 15 h were arrested in G2.phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 2. h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402.involving G2.phase arrest.

关键词： peplomycin Bel-7402.cells apoptosis cyclins cell cycle flow cytometry

Identification of glutamate transporters and receptors in mouse testis

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Acta Pharmacologica Sinica 2004年第03期25卷 112-117页

作者： Jia-hua HU,Na YANG2.Ying-hua MA,Jie JIANG,Jin-fu ZHANG3.Jian FEI4,Li-he GUO4 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.003. 3.epartment of Urology,tongji {3.,tongji university,Shanghai 2.0065,china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Urology Tongji Hospital Tongji University

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.2.) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2., GluR6 and KA2. were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake

A template of rat brain based on fMRI T2. imaging

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Progress in Natural Science:Materials International 2005年第6期15卷 502-506页

作者： HU Zhenghui1, WU Yigen2. WANG Xiaochuan3. WANG Jianzhi3. CHEN Feiyan1 and TANG Xiaowei1*(1. Department of Physics and Bio-X Laboratory, Zhejiang university, Hangzhou 3.002.,{3. 2. Institute of High Energy Physics and Key Laboratory of Nuclear Analysis Techniques, Chinese Academy of Sciences,Beijing 10003.,{3. 3. Department of Pathophysiology, tongji Medical College, Huazhong university of Science and Technology, Wuhan 43.03.,{3.) Department of Physics and Bio-X Laboratory Zhejiang University Hangzhou 310027 China Institute of High Energy Physics and Key Laboratory of Nuclear Analysis Techniques Chinese Academy of Sciences Beijing 100039 China Department of Pathophysiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The development of functional magnetic resonance imaging (fMRI) technology has made it possible to carry out functional brain imaging experiments in small animals. Usually, group data is required to form the assessment of population, which can not only increase the sensitivity of the overall experiment, but also allow the generalization of the conclusion to the whole population. In order to average the signals of functional brain images from different subjects, it is necessary to put all the mapping images into the same standard space (template image). However, up to now, most animal brain templates remain unavailable and it must be done by ourselves. In this study, a template image based on the brains of eight male Wistar rats is obtained, and it is successfully used in our present Alzheimer disease (AD)-like rat model studies as template for spatially normalizing images to the same stereotaxical space. The fMRI results processed with statistical parametric mapping (SPM99) software are in agreement with the results from immunohistochemical experiment, which proves that this method is universally applicable to the pathologic models of other small animals and to human brain lesion studies.

关键词： fMRI animal model atlas template normalization T*2.

NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes

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Acta Pharmacologica Sinica 2004年第6期25卷 12-18页

作者： Bo HU, Sheng-gang SUN2. E-tang TONGNeurology Department, Union hospital, tongji College, Huazhong {2. of Science and Technology, Wuhan 43.02., china Neurology Department Union Hospital Tongji College Huazhong University of Science and Technology Wuhan 430022 China

AIM: To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the roleof N-methyl-D-aspartate (NMDA) and alpha-amino-3.hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) re-ceptors in the procedure. METHODS: The fluorescence of calcium was measured by Fura-2.AM (F3.5/F3.0).RESULTS: L-Glutamate induced [Ca2.]i increase in most of the cells in concentration- and time-dependent *** 50 mmol/L induced the fluorescence increase by almost three to four times, while the effect of AMPA 50mmol/L was just half of that of D-(-)-2.amino-5-phosphonopentanoic acid (D-AP-5; a selective antagonist of theNMDA receptor). 6-Cyano-7-nitroquinoxaline-2.3.dione (CNQX, a selective antagonist of the AMPA receptor)abolished the effects of NMDA and AMPA, respectively. D-AP-5 and CNQX simultaneously or respectively attenu-ated the effect of L-glutamate at different degrees, but could not abolish it entirely. CONCLUSION: Glutamatemodulated intracellular Ca2. of pure cultured rat astrocytes through different pathways. The activation of NMDAand AMPA receptors took part in the complex mechanisms.

关键词： glutamate astrocytes NMDA receptors AMPA receptors calcium

Cytocompatibility of Self-assembled Hydrogel from IKVAV-containing Peptide Amphiphile with Neural Stem Cells

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Journal of Wuhan university of Technology(Materials Science) 2009年第5期24卷 753-756页

作者：宋玉林 Department of Orthopaedics the Second Affiliated Hospital of Nanchang University

Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3.） hydrogels （Experimental Group, EG） or surface of coverslips （Control Group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 2. h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P〈0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.

关键词： IKVAV hydrogel cytocompatibility proliferation

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