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Identification of glutamate transporters and receptors in mouse testis

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Acta Pharmacologica Sinica 2004年第3期25卷 112-117页

作者： Jia-hua HU,Na YANG2.Ying-hua MA,Jie JIANG,Jin-fu ZHANG3,Jian FEI4,Li-he GUO4 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0031 3Department of Urology,{2. hospital,{2. university,shanghai 200065,china 中科院上海生命科学院 Laboratory of Molecular Cell Biology CAS 上海 200031 Department of Urology Tongji Hospital Tongji University 上海 200065

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.2.) pmol/min per mg protein and Km value of glutamate uptake was (1..0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1.selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1.and EAAC1. and ionotropic glutamate receptors (NR1. NR2., GluR6 and KA2. were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake

Establishment of a Screening System for Selection of siRNA Target Sites Directed against Hepatitis B Virus Surface Gene

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 310-316页

作者： Xiu-Min ZHOU~(1.3) Ju-Sheng LIN~1 Yi SHI~3 De-An TIAN~2.Huan-Jun HUANG~2.He-Jun ZHOU~1 You-Xin JIN~3 1 Institute of Liver Diseases. tongji hospital, tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, china: 2.Department of Gastroenterology, tongji hospital, tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, china 3 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, shanghai 2.0031, china Institute of Liver Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Department of Gastroenterology Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China

To study the inhibitory effects of plasmid-derived small interfering RNA(siRNA)andsynthetic siRNA on the expression of the hepatitis B virus surface(HBs)gene,three plasmid-derived siRNAsand one synthetic siRNA that complement the coding region of the HBs gene were *** HBsexpression plasmid pHBs-EGFP was also *** cells were co-transfected with pHBs-EGFPand the above *** HBs mRNA quantities were measured by reverse-transcription PCR,and thelevel of HBs-EGFP fusion protein was quantified by fluorescent *** concentrations of thehepatitis B virus surface antigen(HBsAg)derived from the culture supernatant of transfected HepG2.2.1.cells were measured by an enzyme-linked immunosorbent assay(ELISA)*** results showed that thethree plasmid-derived siRNAs and the synthetic siRNA can effectively reduce the quantities of HBs mRNAand *** plasmid-derived siRNA psiRNA1.was found to be the most effective inhibitor of *** can inhibit HBs-EGFP expression by 63.3% and suppress HBs mRNA by 75.6%.To furthersubstantiate the above observations,psiRNA1.was transfected into HepG2.2.1. cells(an HBV secreting cellline).The transfections resulted in almost complete blockage of HBsAg production,whereas control vector-transfected cells secreted high levels of HBsAg 7 days *** conclusion,our data suggeststhat RNA interference(RNAi)is an efficient approach for reducing the level of HBs transcripts and proteinsand for suppressing HBsAg production.

关键词： RNA interference(RNAi) small interference RNA(siRNA) hepatitis B virus surface gene(HBs gene)

Influence of L-methionine-deprived total parenteral nutrition with 5-fluorouracil on gastric cancer and host metabolism

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World Journal of Gastroenterology 2001年第5期7卷 698-701页

作者： Hong-Bing Xiao~1.Wei-Xin Cao~2.Hao-Ran Yin~2.Yan-Zhen Lin~2.Shi-Hui Ye~1.1.Department of Surgery,Affiliated Railway hospital,tongji {2.,shanghai 2.0072.china2.Department of Surgery,Affiliated Ruijin hospital,shanghai Second Medical {2.,shanghai 2.002.,china Department of Surgery Affiliated Railway Hospital Tongji University Shanghai 200072 China. xhawbin@***

AIM: To investigate the influence of L-methionine-deprived total parenteral nutrition with 5-FU on gastric cancer and host metabolism. METHODS: N-methyl-N'-nitro-nitrosoguanidine (MNNG) induced gastric cancer rats were randomly divided into four groups: Met-containing TPN group (n=1.), Met-deprived TPN group (n =1.0}, Met-containing TPN+5-FU group (n=1.) and Met-deprived TPN+5-FU group (n=1.0}. Five rats in each group were sacrificed after 7 days of treatment and the samples were taken for examination. The remaining rats in each group were then fed separately with normal diet after the treatment until death, the life span was noted. RESULTS: The tumors were enlarged in Met-containing group and shrank in Met-deprived group markedly after the treatment. The DNA index (DI) of tumor cells and the body weight (BW) of rats had no significant change in the two groups, however, the ratio of tumor cells'S phase was increased. The ratio of G2. phase went up in Met-containing group, but down in Met-deprived group. In the other two groups that 5-FU was added, the BW of rats, and the diameter of tumors, the DI of tumor cells, the S and G2. phase ratio of tumor cells were all decreased, particularly in Met-deprived plus 5-FU group. Pathological examination revealed that the necrotic foci of the tumor tissue increased after Met-deprived TPN treatment, and the nucleoli of tumor cells enlarged. In MetTPN+5-FU group, severe nuclear damage was also found by karyopyknosis and karyorrhexis, meanwhile there was slight degeneration in some liver and kidney cells. The serum free Met and Cysteine decreased markedly (P1., while other amino acids, such as serum free serine and glutamine increased significantly (P1..6 days in Met-containing TPN group, 31.days in Met-deprived TPN group, 2..5 days in Met-containing TPN+5-FU group, and 43 days in Met-deprived TPN+5-FU group (P&

关键词： Parenteral Nutrition Animals Antimetabolites, Antineoplastic Body Weight DNA, Neoplasm Fluorouracil Male Methionine Rats Rats, Wistar Research Support, Non-U.S. Gov't S Phase Stomach Neoplasms

The susceptibility of ventricular arrhythmia to aconitine in conscious Lyon hypertensive rats

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Acta Pharmacologica Sinica 2007年第2期28卷 211-215页

作者： Min LI~(2.3) Jin WANG~2.He-hui XIE~2.Fu-ming SHEN~2.Ding-feng SU~(2.4)2.Department of Pharmacology,Second Military.Medical university,shanghai 2.0433,{2. 3 Department of Pediatrics,shanghai Tenth People's hospital,tongji university,shanghai 2.0072.{2. Department of Pharmacology Second Military Medical University Shanghai China Department of Pediatrics Shanghai Tenth People's Hospital Tongji University Shanghai China

Aim:The present work was designed to investigate the relationship betweenhemodynamic parameters and the susceptibility of ventricular arrhythmia toaconitine in conscious Lyon hypertensive rats(LH).Methods:Male LH and Lyonlow blood pressure rats(LL)were *** the determination of baroreflexsensitivity(BRS),ventricular arrhythmia was induced by aconitine infusion inconscious *** pressure(BP)was recorded during the period of ***:Compared with the LL rats,the LH rats possessed significantly higherBP,blood pressure variability and lower *** threshold of aconitine requiredfor ventricular fibrillation and cardiac arrest in the LH rats were significantly lowerthan those in the LL *** was found that all the hemodynamic parametersstudied were not correlated with the threshold of aconitine required for arrhythmia,with the exception of BRS,which was positively related to the threshold ofaconitine required for ventricular premature ***:The LH rats pos-sessed greater susceptibility to aconitine-induced ventricular arrhythmias whencompared to the LL *** greater susceptibility could not be attributed to anyone of the hemodynamic parameters alone studied in the LH *** is proposedthat various hypertension-associated abnormalities,including the abnormalhemodynamics,may co-contribute to this vulnerability to ventricular arrhythmias.

关键词： arrhythmia aconitine baroreflex sensitivity hypertension Lyon hypertensive rats

Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter Ⅰ (GAT1.

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Cell Research 2004年第1期14卷 54-59页

作者： Jia Hua HU~(1,*), Jin Fu ZHANG~(2.*), Ying Hua MA~1, Jie JIANG~1, Na YANG~1, Xin Bo LI~3, Zhi Guang YU CHI~4, Jian FEI~(1,5,**), Li He GUO~(1,**) ~1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, shanghai 2.0031,{2.. ~2.epartment of Urology, tongji hospital, tongji university, 389 Xincun Road, shanghai 200065,{2.. ~3New Drug R & D Center, North{2. Pharmaceutical Group Corporation, 388 Heping East Road, Shijia Zhuang 050015, Hebei,{2.. ~4School of life sciences, Fudan university, 2.0 Handan Road, shanghai 2.0433,{2.. ~5shanghai Research Center for Biomodel Organism, shanghai 2.12.3,{2.. Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China shanghai Research Center for Biomodel Organism Shanghai 201203 China Department of Urology Tongji Hospital Tongji University 389 Xincun Road Shanghai 200065 China New Drug R & D Center North China Pharmaceutical Group Corporation 388 Heping East Road Shijia Zhuang 050015 Hebei China School of life sciences Fudan University 220 Handan Road Shanghai 200433 China

It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABA_A and GABA_B receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter Ⅰ (GAT1. also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1.overexpressing mice to explore GAT1.function in male reproductive system. We found that the expression level of GAT1.continuously increased in wild-type mouse testis from 1.month to 2.months after birth. GAT1.overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1.overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wi

关键词： GABA GAT1 testes sperm reproduction transgenic.

Resting state brain activity and functional brain mapping

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Progress in Natural Science:Materials International 2007年第5期17卷 511-519页

作者： Zhao Xiaohu , Wang Peijun and Tang Xiaowei (College of life Sciences in Zhejiang university, Hangzhou 31.058,{1. {2. Department of Tong Ji hospital of Tong Ji university, shanghai 200065,{1. Bio-X Lab, Department of Physics, Zhejiang university, Hangzhou 31.02.,{1.) College of Life Sciences Zhejiang University Hangzhou 310058 China imaging Department Tongji Hospital Tongji University Shanghai 200065 China Bio-X Lab. Department of Physics Zhejiang University Hangzhou 310027 China

Functional brain imaging studies commonly use either resting or passive task states as their control conditions, and typically identify the activation brain region associated with a specific task by subtracting the resting from the active task conditions. Numerous studies now suggest, however, that the resting state may not reflect true mental "rest" conditions. The mental activity that occurs during "rest" might therefore greatly influence the functional neuroimaging observations that are collected through the usual subtracting analysis strategies. Exploring the ongoing mental processes that occur during resting conditions is thus of particular importance for deciphering functional brain mapping results and obtaining a more comprehensive understanding of human brain functions. In this review article, we will mainly focus on the discussion of the current research background of functional brain mapping at resting state and the physiological significance of the available neuroimaging data.

关键词： resting state brain activity default mode brain networks BOLD deactivation OEF.

Stimulus-responsive polymeric nanoparticles for biomedical applications

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Science china Chemistry 2010年第3期53卷 447-457页

作者： LI YongYong1.2.DONG HaiQing1.2.WANG Kang1.SHI DongLu 2.ZHANG XianZheng1.& ZHUO RenXi1.1.ey Laboratory of Biomedical Polymers,Ministry of Education Department of Chemistry,Wuhan university,Wuhan 430072.china 2.nstitute for Advanced Materials and Nano Biomedicine(iNANO),{2. university,shanghai 2.0092.china Key Laboratory of Biomedical Polymers Ministry of Education Department of Chemistry Wuhan University Wuhan China Institute for Advanced Materials and Nano Biomedicine (iNANO) Tongji University Shanghai China

Polymeric nanoparticles with unique properties are regarded as the most promising materials for biomedical applications including drug delivery and in vitro/in vivo *** them,stimulus-responsive polymeric nanoparticles,usually termed as "intelligent" nanoparticles,could undergo structure,shape,and property changes after being exposed to external signals including pH,temperature,magnetic field,and light,which could be used to modulate the macroscopical behavior of the *** paper reviews the recent progress in stimulus-responsive nanoparticles used for drug delivery and in vitro/in vivo imaging,with an emphasis on double/multiple stimulus-responsive systems and their biomedical applications.

关键词： polymer stimulus-responsive nanoparticles drug carrier cellular imaging

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APPLICATIONS OF CFD TECHNIQUE IN THE DESIGN AND FLOW ANALYSIS OF IMPLANTABLE AXIAL FLOW BLOOD PUMP

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Journal of Hydrodynamics 2010年第4期22卷 518-525页

作者： FAN Hui-min HONG Fang-wen ZHANG Guo-ping YE Liang LIU Zhong-min shanghai East {1. Affiliated to {2. university Shanghai 200120 China china Ship Scientific Research Center Wuxi 214082 China

Based on established numerical methods and hydrodynamic performance testing facilities,CFD technique are applied to improve the design of the implantable axial flow blood pump and the flow analysis.（1. Applying brushless machine magnet steel,reducing its thickness while increasing the length,the flow channel＇s cross-section is increased,with no space connection between the large and the small rotators,and with a cone transition segment from the bearing to the principal axis,the flow is made *** rotating speed is lowered by 1.00 rpm-1.0}0 rpm under the same flow rate and pressure head,and thus the hemolysis can be avoided.（2. Different outlet stator guiding vanes are selected for the same blood pump for analyses of hydrodynamic performances and flow *** excellent design not only can regulate the rotating flow field into an axial one,reduce the circulation loss and improve the pump efficiency,but also can avoid backflow,vortex and secondary flow at the pump outlet,and thus the thrombus can be prevented.（3） The calf live tests show that some residual clots exist at the inner wall of the outlet connection bends,which are analyzed and explained by CFD techniques and the corresponding improvements are *** results are verified by hydrodynamic performance tests and PIV flow field tests,and consistent conclusions are obtained.

关键词： axial flow blood pump CFD PIV

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Novel SLA class I alleles of Chinese pig strains and their significance in xenotransplantation

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Cell Research 2003年第4期13卷 285-294页

作者： Fu XIANG CHEN, JUN TANG, NING LI LI, Bo HUA SHEN, YUN ZHOU, JIN XIE, KUANG YEN CHOUshanghai Institute of Immunology, shanghai Second Medical {2., shanghai 2.002., china Present address: The Oriental hospital, tongji {2., shanghai 2.01.2} shanghai Institute of Immunology Shanghai Second Medical University Shanghai China The Oriental hospital Tongji University Shanghai

To lay background for studying rejection mechanisms in xenotransplantation and developing the strategies for intervention, class I genes of swine leukocyte antigens (SLA) of three Chinese pig strains Bm, Gz and Yn were cloned and sequenced. The cDNA of the class I loci P1.and P1. were amplified by RT-PCR and subjected to insert into sequencing vectors. All six allelic sequences we examined, each two for one Chinese strain, are not identical to those reported, which allows these novel sequences receiving their accession numbers AY1.2.67- AY1.2.72.from GenBank. This study further reveals that the homologies of MHC class I genes in their primary structures and the deduced amino acids between Chinese pigs (SLA) and human (HLA-A*02.1. are better than those between pigs and mice (H-2.b/H-2.b). The comparison also indicates that the amino acid residues critical for recognition by human KIRs are altered in the swine class I molecules. The amino acids responsible for binding human CD8 coreceptor are largely cons

关键词： swine leukocyte antigens (SLA) Chinese pig train xenotransplantation killer cell inhibitory receptor (KIR) CD8 coreceptor.