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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, chinese academy of Sciences, Wuhan 430072.{2. 2. graduate School of chinese academy of Sciences, beijing 100039,{2.) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

Mineralogy and crystal structure determination of Mg-fillowite

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Science china Earth Sciences 2005年第5期48卷 635-646页

作者： MA Zhesheng 1 , SHI Nicheng 1 & YE Danian 2.1. china university of Geosciences, {2. 100083, china 2. Institute of Geology, chinese academy of Sciences, {2. 10002., china china university of Geosciences Beijing China Institute of Geology Chinese Academy of Sciences Beijing China

Accurate crystal structure determination of complex phosphate Mg-fillowite ? Na2.a(Mn4Mg2.e)7[PO4]6 has been finished. Mg-fillowite occurs in muscovite-pegmatite in the Altay area, Xinjiang, china. It is of the trigonal lattice, with space group R-3, unit cell a=1.5143(3) nm, c=4.3191(2. nm, V=8.5736 nm3, Z = 18. The R-factor of the determination accuracy is R (I > 2. ( I )) = 0.0776. The cationic polyhedra consist of two kinds of structural units: compound col- umn and screw column. In three-dimensional space, they are linked with each other through corner sharing or edge sharing to build the entire structure. There are 45 atoms in an asymmetric unit and 72. atoms in a unit cell. In terms of types and connection manners of the coordination polyhedra, the degree of complicity is rarely seen in all mineral crystal structures. Study of such a structure is significant for the classification of phosphate and the building rule of coordination polyhedra in a complex crystal structure.

关键词： Mg-fillowite, complex phosphate, crystal structure, coordination polyhedron.

A 12.bit multichannel ADC for pixel detectors in particle physics and nuclear imaging

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Science china(Technological Sciences) 2010年第5期53卷 1208-1214页

作者： WEI Wei1,2.3,WANG Zheng1,3 & ZHAO JingWei1,3 1 Institute of High Energy Physics,chinese academy of Sciences,{2. 100049,china 2.graduate university of chinese academy of Sciences,{2. 100049,china 3 Key Lab of Nuclear Detection Technology and Nuclear Electronics of chinese academy of Sciences,{2. 100049,china Institute of High Energy Physics Chinese Academy of Sciences graduate university of {2. academy of Sciences Key Lab of Nuclear Detection Technology and Nuclear Electronics of chinese academy of Sciences

Modern pixel detectors in nuclear and particle physics experiments and also in nuclear imaging,starve for highly integrated application specified integrated circuit(ASIC),whereas in china the study of ASIC still stays far away from practical *** lack of ASIC strictly limits the research and development of domestic high energy physics field.A 12.bit multichannel ADC designed for high density readout is introduced as a major candidate for solution.A precise model is discussed and the simulation fully agrees with the model,which indicates a key principle of *** is performed according to the given rule,and novel layout techniques are carried *** results in all aspects are also obtained,showing an excellent real performance,which satisfies the practical requirement.

关键词： application specified integrated circuit high density readout Wilkinson ADC multi-channel ADC

Modeling and verifying Web services driven by requirements:An ontology-based approach

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Science in china(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3,JIN ZHi1,2.& WU Budan1,4 1. academy of Mathematics and Systems Science, chinese academy of Sciences, beijing 100080, china 2. Institute of Computing Technology, chinese academy of Sciences, beijing 100080, china 3. Institute of Software, chinese academy of Sciences, beijing 100080, china 4. graduate university of chinese academy of Sciences, beijing 100049, china academy of Mathematics and Systems Science Chinese Academy of Sciences Institute of Computing Technology Chinese Academy of Sciences Institute of Software Chinese Academy of Sciences graduate university of {2. academy of Sciences

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

Structures and physical properties of rigid polyurethane foams with water as the sole blowing agent

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Science china Chemistry 2006年第4期49卷 363-370页

作者： LI Xiaobin1,2. CAO Hongbin1 & ZHANG Yi1 1. Institute of Process Engineering, chinese academy of Sciences, {2. 100080, china 2. graduate School of the chinese academy of Sciences, {2. 100039, china Institute of Process Engineering Chinese Academy of Sciences Beijing China graduate School of the chinese {2. of Sciences Beijing China

Rigid polyurethane foams blown by varying water level were prepared in this study. The structures and physical properties of rigid polyurethane foams blown by water were measured with FT-IR, universal testing machine (Instron3365), scanning electron microscope (SEM) and differential scanning calorimeter (DSC). The results show that polyurea and polybiuret were the typical charac- teristics, and the cream time and gel time were shorter for the fully water blown rigid foams than that for the fully cyclopentane blown foams. The density of foam samples decreased from 45.0 kg/m3 to 2..4 kg/m3 with the increase of water level from 3 pph to 7 pph. Compressive strength exhibited the similar behavior with density. The average cell size of foam samples ranged from 2.1 μm to 356 μm with the increase of water level from 3 pph to 7 pph, respectively. At the same time, poorer dimen- sional stability was encountered with the increase of average cell size due to fast diffusion rate of CO2.out of the foam. The results of thermal analysis show that the glass transition temperature (Tg) shifted to a higher temperature with the increase of isocyanate level when more water was used as chemical blowing agent.

关键词： polyether polyol, polyurethane rigid foam, compressive strength, dimensional stability, cell size, glass transition temperature.

Explicit analytical solutions of the anisotropic Brinkman model for the natural convection in porous media(Ⅱ)

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Science china(Physics,Mechanics & Astronomy) 2005年第4期48卷 422-430页

作者： CAI Ruixian1, GOU Chenhua1, 2.& ZHANG Na1 1. Institute of Engineering Thermophysics, chinese academy of Sciences, {2. 100080, china 2. graduate School, chinese academy of Sciences, {2. 100080, china Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing China graduate School Chinese Academy of Sciences Beijing China

Some algebraically explicit analytical solutions are derived for the anisotropic Brinkman model―an improved Darcy model―describing the natural convection in porous media. Besides their important theoretical meaning (for example, in analyzing the non-Darcy and anisotropic effects on the convection), such analytical solutions can be the benchmark solutions that can promote the development of computational heat and mass transfer. Some solutions considering the anisotropic effect of permeability have been given previously by the authors, and this paper gives solutions including the anisotropic effect of thermal conductivity and the effect of heat sources.

关键词： explicit analytical solution, porous medium, natural convection, Brinkman model, anisotropic.

Analysis and application of automatic deformation monitoring data for buildings and structures of mining area

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中国有色金属学会会刊：英文版 2011年第S3期21卷 516-522页

作者： XIAO Jie1, 2. 3, ZHANG Jin4 1. Institute of Geodesy and Geophysics, chinese academy of Sciences, Wuhan 430077,{2. 2. Key Laboratory of Dynamic Geodesy, Institute of Geodesy and Geophysics, chinese academy of Sciences, Wuhan 430077,{2. 3. graduate School of chinese academy of Sciences, beijing 100049,{2. 4. Department of Surveying and Mapping, Taiyuan university of Technology, Taiyuan 03002.,{2. Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China Key Laboratory of Dynamic Geodesy Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China graduate School of chinese {2. of Sciences Beijing 100049 China Department of Surveying and Mapping Taiyuan University of Technology Taiyuan 030024 China

The buildings and structures of mines were monitored automatically using modern surveying technology. Through the analysis of the monitoring data, the deformation characteristics were found out from three aspects containing points, lines and regions, which play an important role in understanding the stable state of buildings and structures. The stability and deformation of monitoring points were analysed, and time-series data of monitoring points were denoised with wavelet analysis and Kalman filtering, and exponent function and periodic function were used to get the ideal deformation trend model of monitoring points. Through calculating the monitoring data obtained, analyzing the deformation trend, and cognizing the deformation regularity, it can better service mine safety production and decision-making.

关键词： wavelet analysis Kalman filtering deformation monitoring data analysis mine

Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

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Progress in Natural Science:Materials International 2005年第2期15卷 54-61页

作者： XIA Junhong 1,2. ZHENG Jinsong 1,2. XU Limei 1,2.and WANG Ding1*(1. Institute of Hydrobiology, chinese academy of Sciences, Wuhan 430072.{2. 2. graduate School of the chinese academy of Sciences, beijing 100039,{2.) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China. graduate School of the chinese {2. of Sciences Beijing 100039 China

Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 2. individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

关键词： Yangtze finless porpoise isolated population reproductive behavior parentage

Systematic distortion in cosmic microwave background maps

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Science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 804-808页

作者： LIU Hao 1,3 &LI TiPei 1,2.1Key Laboratory of Particle Astrophysics,Institute of High Energy Physics,chinese academy of Sciences,{2. 100049,china 2.enter for Astrophysics,Tsinghua university,{2. 100084,china 3 graduate university of chinese academy of Sciences,{2. 100049,china Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing China graduate university of {2. academy of Sciences Beijing China Center for Astrophysics Tsinghua University Beijing China

To minimize instrumentally the induced systematic errors,cosmic microwave background(CMB)anisotropy experiments measure temperature differences across the sky using pairs of horn antennas, temperature map is recovered from temperature difference obtained in sky survey through a map-making *** inspect and calibrate residual systematic errors in the recovered temperature maps is important as most previous studies of cosmology are based on these *** analyzing pixel-ring coupling and latitude dependence of CMB temperatures,we find notable systematic devia- tion from CMB Gaussianity in released Wilkinson Microwave Anisotropy Probe(WMAP)*** detected deviation cannot be explained by the best-fit LCDM cosmological model at a confidence level above 99%and cannot be ignored for a precision cosmology study.

关键词： cosmology cosmic microwave background methods data analysis