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Sequence analysis of mRNA polyadenylation signals of rice genes

Chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2. GAO Chenxi1,2.3.& HAN Bin1,3.41. National Center for Gene Research, Shanghai Institutes for Biological sciences, Chinese {3. of sciences, Shanghai 2.02.3. China 2. graduate School of the Chinese {3. of sciences, beijing 10003., China 3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological sciences, Chinese {3. of sciences, Shanghai 2.003.0} China National Center for Gene Research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China graduate School of the Chinese academy of {2. Beijing 100039 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3.-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12.69 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953.genes. The al- ternative poly(A) sites were revealed in approxi- mately 2.% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3. fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3. fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

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Study on the two and three-parameter working stability regions of 3.-TZP suspension

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Science China(Technological sciences) 2006年第5期49卷 547-568页

作者： CHEN Youqiang1, 2. ZHANG Xinni3. CHEN Sha’ou3.& QI Ping3.1. Department of Chemistry, Qingdao university, Qingdao 2.6071, China 2. Department of Chemistry, Tsinghua university, {2. 100084, China 3. Department of Physics, Qingdao university, Qingdao 2.6071, China 1. Department of Chemistry Qingdao University Qingdao 266071 China 2. Department of Chemistry Tsinghua University Beijing 100084 China 3. Department of Physics Qingdao University Qingdao 266071 China

We determined the variation tendency of viscosity (η) at various solids load- ing (α) for 3.-TZP suspension as a function of dispersant concentration (Cw) using vis- cosity measurement, measured the diameter of particles as a function of Cw in very dilute suspension using light scattering method, and obtained the surface features of the sedi- ment of suspension with different Cw using SEM. We also discussed the influence of the microstructure of adsorbed polymer layers on particles and their interactions in the dis- persing medium on the stability of suspension. Then two different stable states and two different unstable states for 3.-TZP suspension were given. Accordingly, the (Cw, η), (Cw, α), and (α, η) two-parameter, and (Cw, α, η) three-parameter stability maps were con- structed. Based on the DLVO theory calculations, the dispersant concentration, particle distance (r), and interparticle potential energy (VT) maps with various solids loading for 3.-TZP aqueous suspension were also constructed. In (Cw, r) two-parameter coordinate system, the stable motion region map for particles was established. The Cw, α, and VT,max (the highest potential energy) surface of the potential barrier map was obtained through calculation and simulation on the basis of the surfaces of Cw, r, VT maps introduced above. The (Cw, α) two-parameter stability map was then obtained from the Cw, α, VT,max map. The results showed that this theoretical map can qualitatively prove the experimentally obtained results—the existence of different dispersed states of particles in suspension system and the variation tendency of suspension stability with changing each parame- ter—were reasonable.

关键词： 3.-TZP, suspension, stability, DLVO theory, interparticle potential energy.

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Modeling of elastic wave propagation on a curved free surface using an improved finite-difference algorithm

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Science China(Physics,Mechanics & Astronomy) 2004年第5期47卷 633-648页

作者： WANG Xiuming1,3.& ZHANG Hailan2.1. CSIRO Petroleum, ARRC, PO BOX 113., Technology Park, Bentley, WA 6102. Australia 2. Institute of Acoustics, Chinese {3. of sciences, {2. 100080, China 3. Present address: Institute of Acoustics, Chinese {3. of sciences, {2. 100080, China 1. CSIRO Petroleum ARRC Technology Park PO BOX 1130 6102 Bentley WA Australia 2. Institute of Acoustics Chinese Academy of Sciences 100080 Beijing China

Based on existing direct and imaging methods of a staggered finite-difference scheme, an improved algorithm for staggered finite-difference is proposed to implement rugged topographic free boundary conditions. This method assumes that the free surface can be implemented with horizontal and vertical free surface segments and their corners; the free surface passes through the grid points of shear stress components, instead of the normal stress components. Imaging is carried out for stress components in both horizontal and vertical directions, thus increasing the accuracy. To update particle- velocities, imaging and updating are first performed in the horizontal direction, and then in the vertical direction. The numerical results for elastic flat horizontal free surface with the imaging method and those for flat free surfaces of various slope angles with the proposed method are compared, and are shown to be in good agreement. The advantage of the proposed method is that only the stresses are dealt with in implementing the free surface into the staggered algorithm, which improves computation efficiency.

关键词： elastic wave simulation, curved free surface, staggered finite-difference, algorithm stability.

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A new principle of synthetic cascade utilization of chemical energy and physical energy

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Science China(Technological sciences) 2005年第2期48卷 163-179页

作者： JIN Hongguang1, HONG Hui1,2. WANG Baoqun1,2. HAN Wei1,2.& LIN Rumou1,2.1. Institute of Engineering Thermophysics, Chinese academy of sciences, {2. 100080, China 2. graduate School of the Chinese academy of sciences, beijing 10003., China 1. Institute of Engineering Thermophysics Chinese Academy of Sciences 100080 Beijing China 2. graduate School of the Chinese academy of {2. 100039 Beijing China

We propose a new principle of the cascade utilization of both chemical energy and physical energy in energy systems with the integration of chemical processes and thermal cycles. Particularly, a general equation of energy levels of substance, Gibbs free energy of chemical reaction and physical energy is explicitly founded. On the basis of this equation, a chemical-looping combustion and an indirect combustion are investigated. Furthermore, a mechanism of energy release, with the combination of decreasing the energy level of Gibbs free energy and upgrading the energy level of low or middle- temperature thermal energy, is clarified. The promising results obtained here establish a theoretical basis for the further investigation of multi-function systems in which energy and the environment are compatible, and create a new approach to improve the performance of traditional thermal cycles.

关键词： chemical energy, cascade utilization, exergy, energy conversion.

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Characteristics of vertical ozone distribution in the lower troposphere in the Yangtze River Delta at Lin'an in the spring of 2.01

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Science China Earth sciences 2005年第9期48卷 1519-1528页

作者： ZHENG Xiangdong1, CHAN Chuenyu2. CUI Hong2. QIN Yu3. CHAN Loyan2. ZHENG Yongguang3.& LEE Yusiang2.1. Chinese academy of Meteorological sciences, {2. 100081, China 2. Department of Civil and Structural Engineering, Hong Kong Polytechnic university, Hung Hom, Hong Kong, China 3. Department of Atmospheric sciences, Peking university, {2. 100871, China 1. Chinese academy of Meteorological sciences 100081 Beijing China 2. Department of Civil and Structural Engineering Hong Kong Polytechnic University Hung Hom Hong Kong China 3. Department of Atmospheric sciences Peking University 100871 Beijing China

We analyzed vertical distributions of ozone (O3. in the lower troposphere (< 5 km above ground) at Lin’an (119.75°E, 3..3.°N), Zhejiang Province using electrochemical concen- tration cell (ECC) ozonesonde data obta... 详细信息

We analyzed vertical distributions of ozone (O3. in the lower troposphere (3..3.°N), Zhejiang Province using electrochemical concen- tration cell (ECC) ozonesonde data obtained from February 2. to April 13. 2.01. The results showed that the vertical O3.distributions are controlled by metrological conditions and the char- acteristics of O3.profiles are related to those of wet bulb potential temperature and wind field. O3.below 2.km showed that the strongest variability and enhanced O3.mixing ratios were associated with easterly winds that blow pollutants from the upwind source region of the Yangtze River Delta (YRD) region. Vertical O3.profiles below 5 km can be grouped into 5 categories: (1) peak mixing ratio type, (2. well-mixed type, (3. layered-structure type, (4) episodic pollution type and (5) alti- tudinal increasing type. Vertical distributions of O3.affected by regional transport of polluted air masses were investigated. Transport of polluted air from high latitudes of northern China, ac- companying subsiding motion of air and stagnant atmospheric conditions are important factors that lead to high mixing ratios of O3.at Lin’an. The stagnant atmospheric conditions associated with a continental high pressure system and pollution plume transported from the YRD and cen- tral-eastern China also lead to regional accumulation of O3.and high O3.mixing ratio at Lin’an. Long-range transport of O3.and pollutants from the Pearl River Delta in South China and in-situ O3.formation also resulted in elevated O3.mixing ratios at around 1 km altitudes and layered O3.distribution in the lower troposphere.

关键词： vertical ozone distribution, lower troposphere, Yangtze River Delta, Lin'an.

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Strengthening of the boreal winter Hadley circulation and its connection with ENSO

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Progress in Natural Science:Materials International 2007年第11期17卷 1327-1333页

作者： Ma Jie1,2.and Li Jianping2.1. College of Atmospheric sciences, Lanzhou {3., Lanzhou 73.000, China 2. LASG, Institute of Atmospheric Physics, Chinese {2. of sciences, beijing 10002., China) College of Atmospheric sciences Lanzhou University Lanzhou 730000 China LASG Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China

Empirical orthogonal function (EOF) analysis is carried out for the year-to-year variability of the boreal winter (DJF) mass stream function of the mean meridional circulation (MMC) during the period 1948—2.05. The results demonstrate that it is dominated by the equatorially asymmetric and symmetric modes. Further analysis shows that the former mode is linked with the boreal winter Hadley cell mainly on the decadal time-scale, and the latter on the interannual time-scale. The asymmetric mode index (AMI) with a clear upward trend contributes to the decadal strengthening of the boreal Hadley circulation, and is closely correlated with the tropical SST warming, especially in the region of Indo-west Pacific warm pool (INWP). Furthermore, the AMI also contributes to the abrupt change of the correlation coefficient between the boreal Hadley circulation and ENSO after 1976. The symmetric mode index (SMI) with robust and stable linkage with ENSO shows a significant interannual variability, suggesting that the variability of the Hadley circulation is mainly associated with ENSO on the interannual time-scale.

关键词： the Hadley circulation interannual time-scale decadal time-scale

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Pattern phenomena in an rf discharge dusty plasma system

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Science China(Physics,Mechanics & Astronomy) 2006年第5期49卷 588-596页

作者： HUANG Feng1,2. YE Maofu2.& WANG Long2.1. College of Science, China Agricultural {3., beijing 100083. China 2. Institute of Physics, Chinese {2. of sciences, beijing 100080, China 1. College of Science China Agricultural University Beijing 100083 China 2. Institute of Physics Chinese Academy of Sciences Beijing 100080 China

Various dust patterns are observed in an rf discharge dusty plasma system. According to the dust growth process from small to large in size, the formation of different dust patterns can be divided into two stages: the small-particle stage (or dust cloud stage), and the large-particle stage (or dust crystal stage). The evolution relations between different dust patterns with gas pressure changing are investigated. Dust voids, dust acoustic waves and strong turbulence modes are presented at the small-particle stage. The self-organized dust lattices and dust clusters are investigated at the large-particle stage. The static structure of a dust lattice is characterized by means of the pair correlation function. Dust clusters formed by particles with different numbers and the regular evolution of the clusters with gas pressure are also investigated. The packing sequences of dust clusters are verified through two-dimensional confined molecular dynamics simulations.

关键词： dusty plasma dust patterns

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Global analysis theory of climate system and its applications

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Chinese Science Bulletin 2003年第10期48卷 1034-1039页

作者： LI Jianping1,2.& CHOU Jifan2.3.1. LASG, Institute of Atmospheric Physics, Chinese academy of {3., {2. 10002., China 2. Department of Atmospheric {3., Lanzhou university, Lanzhou 73.000, China 3. Training Center for China Meteorological Administration, {2. 100081, China Department of Atmospheric sciences Lanzhou University Lanzhou 730000 China LASG Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China

The idea and main theoretical results of the global analysis theory of climate system are briefly summarized in this paper. A theorem on the global behavior of climate system is given, i.e. there exists a global attractor in the dynamical equations of climate, any state of climate system will be evolved into the global attractor as time increases, indicating the nonlinear adjustment process of climate system to external forcing. The different effects of external forcing, dissipation and nonlinearity on the long-term behavior of solutions are pointed out, and some main applications of the global analysis theory are also introduced. Especially, three applications, the adjustment and evolution processes of climate, the principle of numerical model design and the optimally numerical integration, are discussed.

关键词：整体分析理论气侯体系应用不定性计算法则气侯科学数字模型

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Mineralogy and crystal structure determination of Mg-fillowite

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Science China Earth sciences 2005年第5期48卷 635-646页

作者： MA Zhesheng 1 , SHI Nicheng 1 & YE Danian 2.1. China university of Geosciences, {2. 100083. China 2. Institute of Geology, Chinese academy of sciences, {2. 10002., China 1. China university of Geosciences 100083 Beijing China 2. Institute of Geology Chinese Academy of Sciences 100029 Beijing China

Accurate crystal structure determination of complex phosphate Mg-fillowite ? Na2.a(Mn4Mg2.e)7[PO4]6 has been finished. Mg-fillowite occurs in muscovite-pegmatite in the Altay area, Xinjiang, China. It is of the trigonal lattice, with space group R-3. unit cell a=1.5143.3. nm, c=4.3.91(2. nm, V=8.573. nm3. Z = 18. The R-factor of the determination accuracy is R (I > 2. ( I )) = 0.0776. The cationic polyhedra consist of two kinds of structural units: compound col- umn and screw column. In three-dimensional space, they are linked with each other through corner sharing or edge sharing to build the entire structure. There are 45 atoms in an asymmetric unit and 72. atoms in a unit cell. In terms of types and connection manners of the coordination polyhedra, the degree of complicity is rarely seen in all mineral crystal structures. Study of such a structure is significant for the classification of phosphate and the building rule of coordination polyhedra in a complex crystal structure.

关键词： Mg-fillowite, complex phosphate, crystal structure, coordination polyhedron.

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Isotopic feature and uranium dating of the volcanic rocks in the Okinawa Trough

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中国科学：地球科学英文版 2006年第4期49卷 375-383页

作者： HUANG Peng1,2. LI Anchun1, HU Ningjing2.3. FU Yongtao1& MA Zhibang4 1. Institute of Oceanology, Chinese academy of {3., Qingdao 2.6071, China 2. {2. School of the Chinese academy of {3., beijing 10003., China 3. Institute of Soil Science, Chinese academy of {3., Nanjing 2.0008, China 4. Institute of Geology and Geophysics, Chinese academy of {3., beijing 10002., China 1. Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 2. graduate School of the Chinese academy of {2. Beijing 100039 China 3. Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 China 4. Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

Volcanic rocks from the northern and middle Okinawa Trough were dated by ura- nium-series dating method. Differential fractions using magnetic procedure were designed to separate samples. New report on the ages and isotopic data of rocks in the northern trough (especially black pumice) was discussed. Based on the uranium dates and Sr-Nd isotopic ratio, magmatic evolution process of the Okinawa Trough was noted. Firstly, there have been wide silicic volcanic activities in the Okinawa Trough from late Pleistocene to present, and the volcanic rocks can be divided into three subgroups. Secondly, magma generally came from PREMA source area under the Okinawa Trough. Magmatic evolution in the northern trough was similar to the middle, but different to the south. Finally, volcanic activities indicated that opening of the southern Okinawa Trough did not happen due to the collision between Luson Arc and Eurasian Plate until the early Pleistocene.

关键词： Okinawa Trough, black pumice, uranium-series dating, Sr-Nd isotopic ratio, magmatic evolution.

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