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Molecular identification of species in Prunus sect. Persica (Rosaceae), with emphasis on evaluation of candidate barcodes for plants

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Journal of Systematics and Evolution 2011年第2期49卷 138-145页

作者： 1,2.Xu QUAN 1 Shi-Liang ZHOU 1 (State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, chinese academy of Sciences, {2. 100093, china) 2.(graduate school of the chinese academy of Sciences, {2. 100049, china) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (graduate school of the {2. academy of Sciences Beijing 100049 China)

Species of Prunus L. sect. Persica are not only important fruit trees, but also popular ornamental and medicinal plants. Correct identification of seedlings, barks, or fruit kernels is sometimes required, but no reliable morphological characters are available. Nowadays, the technique of DNA barcoding has the potential to meet such requirements. In this study, we evaluated the suitability of 11 DNA loci (atpB-rbcL, trnH-psbA, trnL-F, trnS-G, atpF-H, rbcL, matK, rpoB, rpoC1, nad1, and internal transcribed spacer [ITS]) as candidate DNA barcodes for peaches, using samples from 38 populations, covering all the species in sect. Persica. On the whole, the primers worked well in this group and sequencing difficulties were met only in the case of ITS locus. Five loci (rbcL, matK, rpoB, rpoC, and nad1) have very low variation rates, whereas atpB-rbcL, atpF-H, trnH-psbA, trnL-F and trnS-G show more variability. The most variable loci, atpB-rbcL and trnH-psbA, can distinguish three of the five species. Two two-locus combinations, atpB-rbcL+trnL-F and atpB-rbcL+atpF-H, can resolve all five species. We also find that identification powers of the loci are method-dependent. The NeighborNet method shows higher species identification power than maximum parsimony, neighbor joining, and unweighted pair group method with arithmetic mean methods.

关键词： atpB-rbcL DNA barcode Prunus sect. Persica trnL-F.

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Observation results of relativistic electrons detected by Fengyun-1 satellite and analysis of relativistic electron enhancement (REE) events

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Science china(Physics,Mechanics & Astronomy) 2008年第12期51卷 1947-1956页

作者： YANG XiaoChao1,2. & WANG ShiJin1 1 Center for Space Science and Applied Research, chinese academy of Sciences, {2. 100190, china 2.graduate University of chinese academy of Sciences, {2. 100049, china Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China graduate University of chinese {2. of Sciences Beijing China

The space particle component detector on Fengyun-1 satellite which works at the sun-synchronous orbit of about 870 km altitude has detected relativistic electrons for a long time. In comparison with the SAMPEX satellite observations during 1999–2.04, the relativistic electron data from Fengyun-1 satellite from June 1999 to 2.05 are used to analyze the relativistic electron enhancement (REE) events at the low earth orbit, and the possible correlation among REE events at the low earth orbit, high-speed solar wind and geomagnetic storms is discussed. The statistical result presents that 45 REE events are found in total during this time period, and the strong REE events with the maximum daily average flux > 400 cm?2.srt-1·s?1 occur mostly during the transition period from solar maximum to solar minimum. Among these 45 REE events, four strong REE events last a longer time period from 2.-to 51-day and correlate closely with high speed solar wind and strong geomagnetic storms. Meanwhile, several strong geomagnetic storms occur continuously before these REE events, and these continuous geomagnetic storms would be an important factor causing these long-lasting strong REE events. The correlation analysis for overall 45 events indicates that the strength of the REE events correlates with the solar wind speed and the strength of the geomagnetic storm, and the correlation for strong REE events is much stronger than that for weak REE events.

关键词： relativistic electron enhancement low earth orbit high-speed solar wind geomagnetic storm

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Cloning and Characterization of β-Carotene Ketolase Gene Promoter in Haematococcus pluvialis

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 270-275页

作者： Chun-Xiao MENG~(1,2. Chang-Ying TENG~1 Peng JIANG~1 Song QIN~1 Cheng-Kui TSENG~1 ~1Institute of Oceanology,chinese academy of Sciences.Qingdao 2.6071,{2. ~2.3} school of the chinese academy of Sciences,beijing 100039.{2. Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China graduate school of the {2. academy of Sciences beijing 100039 China

The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid,astaxanthin,under various environmental stresses.β-carotene ketolase (BKT) plays a key role in astaxanthinbiosynthesis in *** this paper,an approximate 700 bp 5'-flanking region of the bkt gene contain-ing a putative promoter was cloned through walking *** results of the sequence analysis showedthat this bkt 5'-flanking region might have cis-acting elements such as sterol regulatory element(SRE-1)-likemotifs,the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like *** for typical TATA and CCAAT *** results of the β-galactosidase assay and the transientexpression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like regionmight exist from-630 to-408 bp,and the highest promoter activity was observed to span the positionsfrom-630 to-308 *** results of the site-directed mutagenesis of a C-repeat/DRE and two APE-likemotifs in a promoter-like region(-630 to -308 bp)suggested that two APE-like motifs might be essential fortranscriptional control of the bkt gene.

关键词： Haematococcus pluvialis astaxanthin β-carotene ketolase gene (bkt) cis-acting element promoter

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De-interlacing technique based on total variation with spatial-temporal smoothness constraint

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Science in china(Series F) 2007年第4期50卷 561-575页

作者： YIN XueMin1,2.3, YUAN JianHua1,2. LU XiaoPeng1,2.& ZOU MouYan1,2.1 Institute of Electronics, chinese academy of Sciences, {2. 100080, china 2.graduate school, chinese academy of Sciences, beijing 100039, china 3 Jiuquan Satellite Launch Center, Lanzhou 732.50, china Institute of Electronics Chinese Academy of Sciences Beijing China graduate school Chinese Academy of Sciences Beijing China Jiuquan Satellite Launch Center Lanzhou China

This paper introduces a new method of converting interlaced video to a progressively scanned video and image, The new method is derived from Bayesian framework with the spatial-temporal smoothness constraint and the MAP is done by minimizing the energy functional, The half-quadratic regularization method is used to solve the corresponding partial differential equations （PDEs）, This approach gives the improved results over the conventional de-interlacing methods, Two criteria are proposed in the paper, and they can be used to evaluate the performance of the de-interlacing algorithms,

关键词： video processing de-interlacing total variation spatio-temporai smoothness constraint PDEs half-quadratic regularization

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Effects of diatoms on reproduction and growth of marine copepods

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Progress in Natural Science:Materials International 2004年第10期14卷 1-7页

作者： ZHANG Guangtao 1,2. and SUN Song 1 (1.Key Laboratory of Marine Ecology&Environment Sciences,Institute of Oceanology,chinese academy of Sciences,Qingdao2.6071,{2. 2.graduate school,chinese academy of Sciences,beijing100039,{2.) Lab. of Marine Ecology Inst. of Oceanology Chinese Acad. of Sci. Qingdao 266071 China graduate Sch. Chinese Acad. of Sci. Beijing 100039 China

Two hot spots in marine ecology,deleterious effects of diatoms and feeding selectivity of copepods,as well as new progress on these two issues achieved in the recent ten years,are *** two issues are considered correlated *** and their metabolites can induce deleterious effects on growth,reproduction and development of copepods,including increase of mortality and decrease of egg production,hatching and growth *** negative effects,resulting from either chemical toxin or nutritional defi-ciency,can be conquered in natural environments by diverse *** is therefore concluded that deleterious effects of diatoms observed in laboratory or during blooming period are only a special case that accommodation of feeding strategy of copepods is *** under-stand their feeding strategy in natural environments is a prerequisite to explaining the mechanisms of deleterious effects caused by diatoms,and makes it possible to re-evaluate the energy flow in marine ecosystems.

关键词： diatoms copepod feeding strategy reproduction deleterious effects

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Research progress in the inner surface modification of metal tubes by plasma processing

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Progress in Natural Science:Materials International 2006年第11期16卷 1119-1126页

作者： ZHANG Guling~ 1,2.**, WU Xingfang~1, GU Weichao~2. CHEN Guangliang~2. FENG Wenran~2. NIU Erwu~2. LI Li~2. L Guohua~2. CHEN Huan~2. FAN Songhua~2. LIU Chizi~2.and YANG Size~2.1. school of Material Science and Engineering, University of Science and Technology beijing, beijing 100083, china 2. beijing National Laboratory for Condensed Matter Physics, Institute of Physics, chinese academy of Sciences, beijing 100080, china) school of Material Science and Engineering University of Science and Technology Beijing Beijing 100083 China beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The recent progress in inner surface hardening of tubes by plasma processing is summerized. Several techniques of inner surface plasma source ion implantation and deposition are introduced, and their advantages and disadvantages are discussed. The basic principles, technical features and new progress of inner surface plasma source ion implantation methods for metal tubes, which were developed in our laboratory, are described in detail. And perspectives of the future technical development for inner surface ion implantation of tubes are presented.

关键词： PSII metal tubes inner surface surface modification.

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Preparation of Pt/K_2.a_2.i_3O_(10) and its photo-catalytic activity for hydrogen evolution from methanol water solution

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Science china Chemistry 2006年第2期49卷 162-168页

作者： CUI Wenquan1,3, LIU Li2. FENG Liangrong1, XU Chenghua1, LI Zijian1, Lü Shaojie1 & QIU Fali1 1. Chengdu Institute of Organic Chemistry, chinese academy of Sciences, Chengdu 610041,{2. 2. Chemistry Department, Sichuan University of Science & Engineering, Zigong 643033,{2. 3. graduate school of the chinese academy of Sciences, beijing 100039,{2. Correspondence should be addressed to Qiu Fali Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu China graduate school of the {2. academy of Sciences Beijing China Chemistry Department Sichuan University of Science & Engineering Zigong China

A layered perovskite-type oxide K2.a2.i3O10 was prepared with high-temperature solid-state reaction, and characterized with X-ray diffraction (XRD) and ultraviolet-visible diffuse re-flectance spectroscopy (UV-Vis DRS). Platinum was loaded onto K2.a2.i3O10 as a co-catalyst with different methods. The dispersion of platinum on K2.a2.i3O10 was determined with HOT method. The photocatalytic activity of Pt/K2.a2.i3O10 was studied. This catalyst showed much higher activity in hydrogen evolution reaction than Ni/K2.a2.i3O10. The effects of different amounts of loaded platinum and several preparation methods were evaluated. The best achieved hydrogen evolution rate was 2.3.88 μmol·h?1 by Pt(2.)/K2.a2.i3O10. A possible band structure and mechanism were discussed based on the results.

关键词： photocatalysis hydrogen platinum K_2.a_2.i_3O_(10) support

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Comparative study of pollination biology of two closely related alpine Primula species,namely Primula beesiana and *** (Primulaceae)

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Journal of Systematics and Evolution 2010年第2期48卷 109-117页

作者： 1,2.hi-Kun WU 1Chang-Qin ZHANG 1(Kunming Institute of Botany,chinese academy of Sciences,Kunming 6502.4,{2.) 2.graduate school of chinese academy of Sciences,beijing 100049,{2.) (Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (graduate school of {2. academy of Sciences Beijing 100049 China)

The comparative pollination biology of a population of Primula beesiana,a population of ***,and an overlapping population of these two species in Yulong Shan,Yunnan Province,china,was studied in 2.04 and *** results indicate that both *** and *** are typical heterostylous and obligate outcrossing species;the main pollinators of the two species were bees and *** the sites of all three populations,the main pollinating visitors of the two species showed preference for one of the two species,resulting in the ethological isolation of the two species by the *** ethological isolation contributes to the reproductive isolation of the two species,which supports the hypothesis that *** and *** are two distinct *** reproductive isolation of the two species (ethological isolation) is probably an important mechanism in maintaining species boundaries in the genus and has contributed to the species diversification of Primula in the *** addition,gene exchanges between *** and *** has occurred to some extent in the overlapping population,but whether natural hybridization has contributed to species diversification in Primula remains to be determined.

关键词： pollination biology Primula beesiana Primula bulleyana reproductive isolation

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An improved imaging algorithm for fixed-receiver bistatic SAR

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Science china(Information Sciences) 2010年第7期53卷 1461-1469页

作者： GENG XuPu1,2.3, HU YingHui1,2. YAN HongHui2.& WANG YanFei2.1graduate school of the {2. academy of Sciences, beijing 100039, china 2.nstitute of Electronics, {2. academy of Sciences, beijing 100190, china 3beijing Institute of Environment Features, beijing 100854, china graduate school of the {2. academy of Sciences Beijing China Institute of Electronics Chinese Academy of Sciences Beijing China beijing Institute of Environment Features Beijing China

Based on the signal model of fixed-receiver bistatic SAR, this paper analyzes the characteristics of azimuth-variant, which includes variation of range migration curve and variation of Doppler rate caused by different azimuth position of targets. Then, an extended nonlinear chirp scaling (NLCS) algorithm is proposed, with its flowchart and phase compensation functions being provided. Simulation results show that the algorithm is available for the characteristics of azimuth-variant of fixed-receiver bistatic SAR, and good focused radar images could be acquired.

关键词： synthetic aperture radar (SAR) bistatic SAR fixed-receiver characteristics of azimuth-variant range cell migration correction (RCMC)

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Sporopollen analysis of Core B10 in the southern Yellow Sea and the reflected characteristics of climate changes

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chinese Science Bulletin 2003年第B6期48卷 42-48页

作者： FU Mingzuo~(1,2.3), LI Zhen~1, XU Xiaowei~1 & SHl Xuefa~1 1. Key Laboratory of Marine Sedimentology and Environmental Geology, First institute of Oceanography. State Oceanic Administration, Qingdao 2.6061. china 2. Institute of Oceanology, chinese {2. of Sciences, Qingdao 2.6071. china 3. graduate school, chinese {2. of Sciences, beijing 100039. china Key Laboratory of Marine Sedimentology and Environmental Geology First institute of Oceanography State Oceanic Administration Qingdao China Institute of Oceanology Chinese Academy of Sciences Qingdao China graduate school Chinese Academy of Sciences Beijing China

Eight sporopollen zones have been divided based on the results of high-resolution sporopollen analysis of Core B10 in the southern Yellow Sea. Based on the results along with ^14C datings and the subbottom profiling data,climatic and environmental changes since the last stage of late Pleistocene are discussed. The main conclusions are drawn as follows: (1) the vegetation evolved in the process of coniferous forest-grassland containing broad-leaved trees→coniferous and broad-leaved mixed forest→coniferons and broad-leaved mixed forest-grassland prevailed by coniferous trees→coniferous and broad-leaved mixed forest-grassland containing evergreen broad-leaved trees→coniferons and broad-leaved mixed forest-grassland prevailed by broadleaved trees→deciduous broad-leaved forest-meadow containing evergreen broad-leaved trees→coniferous and broadleaved mixed forest-grassland prevailed by broad-leaved trees→coniferous and broad-leaved mixed forest containing evergreen broad-leaved trees; (2. eight stages of climate changes are identified as the cold and dry stage, the temperate and wet stage, the cold and dry stage, the warm and dry stage, the temperate and wet stage, the hot and dry stage, the temperate and dry stage, then the warm and dry stage in turn; (3) the sedimentary environment developed from land,to littoral zone, to land again, then to shore-neritic zone; and (4) the Yellow Sea Warm Current formed during early-Holocene rather than Atlantic stage.

关键词：中国黄海气候变化碳14年代测定底基压型数据更新世沉积环境大西洋

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