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Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2. Zhou Xiuxia1,2. Huang Rong1,2.and Guo Qionglin1(1. Institute of Hydrobiololgy, Chinese academy of sciences, Wuhan 430072.{2. 2. graduate University of Chinese academy of sciences, beijing 100039,{2.) Institute of Hydrobiololgy Chinese Academy of Sciences Wuhan 430072 China graduate University of Chinese academy of {2. Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352.amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2.recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

Application of three-dimensional discrete element face-to-face contact model with fissure water pressure to stability analysis of landslide in Panluo iron mine

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Science china(Technological sciences) 2005年第z1期48卷 146-156页

作者： ZHANG Lei1,2. WEI Zuoan1, LIU Xiaoyu1 & LI Shihai1 1.Institute of Mechanics, Chinese academy of sciences, {2. 100080, china 2.graduate school, Chinese academy of sciences, beijing 100060, china Chinese Acad Sci Inst Mech Beijing 100080 Peoples R China Chinese Acad Sci Grad Sch Beijing 100060 Peoples R China

Three-dimensional discrete element face-to-face contact model with fissure water pressure is established in this paper and the model is used to simulate three-stage process of landslide under fissure water pressure in the opencast mine, according to the actual state of landslide in Panluo iron mine where landslide happened in 1990 and was fathered in 1999. The calculation results show that fissure water pressure on the sliding surface is the main reason causing landslide and the local soft interlayer weakens the stability of slope. If the discrete element method adopts the same assumption as the limit equilibrium method, the results of two methods are in good agreement; while if the assumption is not adopted in the discrete element method, the critical φ numerically calculated is less than the one calculated by use of the limit equilibrium method for the sameC. Thus, from an engineering point of view, the result from the discrete element model simulation is safer and has more widely application since the discrete element model takes into account the effect of rock mass structures.

关键词： face-to-facecontactmodel, fissure water pressure, three-dimensional discrete element method,landslide.

Variation properties of ionospheric eclipse factor and ionospheric influence factor

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Progress in Natural Science:Materials International 2005年第6期15卷 573-576页

作者： ZHAO Chunmei 1,2.*, YUAN Yunbin1, OU Jikun1 and CHEN Jinping3(1. Institute of Geodesy and Geophysics, Chinese academy of sciences, Wuhan 430077,{2. 2. graduate school of Chinese academy of sciences, beijing 100039,{2. 3. Department of Precision Instrument and Mechanology, Tsinghua University, beijing 100084,{2.) Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China graduate school of Chinese {2. of sciences Beijing 100039 China Department of Precision Instrument and Mechanology Tsinghua University Beijing 100084 China

The concepts and calculation methods of ionospheric eclipse factor (IEF) and ionospheric influence factor (IFF) are further illustrated. The temporal and spacial variation properties of IEF and IFF are studied, which shows that the properties are influenced by the geographic position and season. The possibility of improving the precision of using GPS data to determine ionospheric delay based on the above variation properties is also analysed.

关键词： GPS ionospheric eclipse factor ionospheric influence factor

Gasdynamic characteristics of toroidal shock and detonation wave converging

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Science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 739-749页

作者： TENG Honghui1,2.& JIANG Zonglin2.1. Key Laboratory of High Temperature Gas Dynamics, Institute of Mechanics, Chinese academy of Sci- ences, beijing 100080,{2. 2. graduate University of Chinese academy of sciences, beijing 100039,{2. 1. Key Laboratory of High Temperature Gas Dynamics Institute of Mechanics Chinese Academy of Sciences 100080 Beijing China 2. graduate University of Chinese academy of {2. 100039 Beijing China

The modified CCW relation is applied to analyzing the shock, detonation wave converging and the role of chemical reactions in the process. Results indicate that the shock wave is strengthened faster than the detonation wave in the converging at the same initial Mach number. Euler equations implemented with a detailed chemical reaction model are solved to simulate toroidal shock and detonation wave converging. Gasdynamic characteristics of the converging are investigated, including wave interaction patterns, observable discrepancies and physical phenomena behind them. By comparing wave diffractions, converging processes and pressure evolutions in the focusing area, the different effects of chemical reactions on diffracting and converging processes are discussed and the analytic conclusion is demonstrated through the observation of numerical simulations.

关键词： converging, CCW relation, shock wave, detonation wave.

Experimental and discrete element numerical analysis of side slope instability induced by fissure water underlying impervious bed

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Science china(Technological sciences) 2005年第z1期48卷 65-80页

作者： LIU Bingshan1, LI Shihai1, ZHANG Lei1,2.& WANG Jianguo31. Institute of Mechanics, Chinese academy of sciences, {2. 100080, china 2. graduate school, Chinese academy of sciences, beijing 100060, china 3. Tropical Marine Science Institute, National University of Singapore, 10 Kent Ridge Crescent, Singapore 1192.0 Chinese Acad Sci Inst Mech Beijing 100080 Peoples R China Chinese Acad Sci Grad Sch Beijing 100060 Peoples R China Natl Univ Singapore Trop Marine Sci Inst Singapore 119260 Singapore

When the sliding mass contains impervious bed, rainfall can infiltrate into mountain via crevices and form higher artesian aquifer at impervious bed inferior. This will decrease slip resistance and increase sliding forces of the sliding mass, thus lowering the safety factor, and inducing landslide disasters. In this paper, a landslide experimental apparatus is designed for experimental studies on the mechanism of this type of landslides. Meanwhile, the non-dimensional parameters in the model experiment are taken into account using dimensional analysis. The experimental results show that (1) the ratio of the cleft water pressure to the overlying pressure is a crucial parameter affecting the stability of the slope; (2. when the shut-in pressure reaches 80% of the normal component of the pressure on the slip surface made up of rock and soil, landslide will occur; (3) the whole slope will start to slide when the shut-in pressure is equal to the normal component of the pressure formed by the overlying rock and soil on the upper 30% area. In this article, a discrete element method simulation is used to investigate the influence of cleft water pressure and shearing strength on the landslide stability. It can be concluded that the critical value of Ccr, ?cr, which determines the slide mass stability, increases with the increase of the water pressure; if the water pressure reaches a high level, the stability of the slide mass depends mainly on C, while the influence of ? becomes smaller than C.

关键词： impervious bed, rainfall infiltration, pore water pressure, landslide experiment, displacement monitor, discrete element method.

Nonlinear interactions between gravity waves and background winds

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Progress in Natural Science:Materials International 2007年第6期17卷 639-644页

作者： Liu Xiao1,2.and Xu Jiyao1(1. State Key Laboratory for Space Weather, Center for Space Science and Applied Research, Chinese academy of sciences, {2. 100080, china 2. graduate University of Chinese academy of sciences, {2. 100039, china) [a]State Key Laboratory for Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China [b]graduate University of Chinese academy of {2. Beijing China

Using the nonlinear propagating gravity waves (GW) model in the two-dimensional compressible atmosphere and the linear GW theory, the process of GW propagation in different background winds, e.g. the direction of the background wind is opposite to (dead wind) or the same as (tail wind) the direction of the horizontal phase velocity of GW, is studied. The results show that the dead wind prolongs the vertical wavelength and accelerates GW propagation. Therefore, GW propagates up to a higher height becomes instable in a short time and eventually induces an inverse jet flow. Then, the vertical wavelength is becoming short due to the nonlinear interactions between GW and the inverse jet flow. The vertical wavelength and group velocity decrease after GW propagates into the tail wind. The initial instable time is delayed. Although most of GW is trapped in the instable region, some of GW propagates above the instable region. Compared with GW propagation in the tail wind, the nonlinear interactions between GW and the dead wind are also strong. In contrast, the linear GW theory predicts that GW can propagate freely in the dead wind. The vertical wavelength simulated by the nonlinear numerical model is different from that predicted by the linear theory greatly after GW propagates into the dead wind.

关键词： gravity waves background winds vertical group velocity vertical wavelength nonlinear interactions.

Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2.3, Lei Gang1,2. Wuriyanghan Hada1,2. Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese academy of sciences, {2. 100101, china 2. graduate school of Chinese academy of sciences, {2. 100039, china 3. Xinjiang academy of Agricultural and Reclamation Science, Shihezi 832.00, china) National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China graduate school of Chinese {2. of sciences Beijing 100039 China Xinjiang academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2.and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2.and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

Improvement of different source function expressions in SWAN model for the Bohai Sea

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Progress in Natural Science:Materials International 2005年第8期15卷 720-724页

作者： YIN Baoshu1*, YANG Dezhou1, SHA Rina 1,2.and CHENG Minghua1(1. Institute of Oceanology, Chinese academy of sciences, Qingdao 2.6071,{2. 2. graduate school of the Chinese academy of sciences, beijing 100039,{2.) Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China. graduate school of the Chinese {2. of sciences Beijing 100039 China

The simulating wave nearshore （SWAN） wave model has been widely used in coastal areas, lakes and estuaries. However, we found a poor agreement between modeling results and measurements in analyzing the chosen four typical cases when we used the default parameters of the source function formulas of the SWAN to make wave simulation for the Bohai Sea. Also, it was found that at the same wind process the simulated results of two wind generation expressions （Komen, Janssen） demonstrated a large difference. Further study showed that the proportionality coefficient α in linear growth term of wave growth source function plays an unperceived role in the process of wave development. Based on experiments and analysis, we thought that the coefficient α should change rather than be a constant. Therefore, the coefficient α changing with the variation of friction velocity U*was introduced into the linear growth term of wave growth source function. Four weather processes were adopted to validate the improvement in the linear growth term. The results from the improved coefficient α agree much better with the measurements than those from the default constant coefficient α. Furthermore, the large differences of results between Komen wind generation expression and Janssen wind generation expression were eliminated. We also experimented with the four weather processes to test the new white-capping mechanisms based on the cumulative steepness method. It was found that the parameters of the new white-capping mechanisms are not suitable for the Bohai Sea, but Alkyon's white-capping mechanisms can be applicable to the Bohai Sea after amendments, demonstrating that this improvement of parameter α can improve the simulated results of the Bohai Sea.

关键词： waves SWAN model wave growth source function new proportionality coefficient formula.

Spectral analysis of quasi-stationary sea surface topography from GRACE mission

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Science china Earth sciences 2005年第11期48卷 2040-2048页

作者： ZHANG Zizhan1,2.& LU Yang1,3 1. Institute of Geodesy and Geophysics, Chinese academy of sciences, Wuhan 430077,{2. 2. graduate school of the Chinese academy of sciences, beijing 100039,{2. 3. Unite Center for Astro-geodymics Research, Chinese academy of sciences, Shanghai 2.0030,{2. 1. Institute of Geodesy and Geophysics Chinese Academy of Sciences 430077 Wuhan China 2. graduate school of the Chinese academy of {2. 100039 Beijing China 3. Unite Center for Astro-geodymics Research Chinese Academy of Sciences 200030 Shanghai China

During the last two decades satellite altimetry has offered an abundance of meas-urements of the sea surface resulting in the improvement of global mean sea surface height (MSSH) and marine geoid determination. On the other hand, with the launching of new genera-tion gravity satellites, some high accuracy long-wavelength gravity models are available. These breakthroughs give us a great opportunity for new estimation of quasi-stationary sea surface topography (QSST). In this paper, the new gravity model GGM01C derived from GRACE mission is briefly presented, and a new global high precision and high-resolution QSST is determined based on the GGM01C model and the global MSSH. The spectral features of the QSST esti-mated by GGM01C and EGM96 gravity model to degree/order 2.0 are discussed by spectral analysis. As a result, the QSST is mainly composed of long waves, medium waves partially and short waves scarcely, its power spectral structures are different between the zonal direction and the meridional direction, there are great differences between the two models, which maybe ex-plain why the ocean currents derived from the two gravity models by Tapley show different pat-terns.

关键词： quasi-stationary sea surface topography, mean sea surface height, GGM01, fast Fourier transform, spectral analysis