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联苯胺对鲫鱼肝脏线粒体影响的微量热研究

武汉大学学报（理学版） 2003年第4期49卷 437-440页

作者：戴捷周培疆朱军成刘鹏刘义武汉大学资源与环境科学学院武汉大学化学与分子科学学院湖北武汉430072

用TAMair等温微量热检测系统 ,测定鲫鱼 (Carassiusauratus)肝脏线粒体体外代谢热及不同浓度联苯胺 (1.0 ,5 .0 ,10 .0 ,2.0 .0 ,4 0 .0mg/L)对其代谢活性的影响 .结果表明 ,线粒体体外代谢活性恢复期速率常数k1,活性衰竭期速率常数k2.... 详细信息

用TAMair等温微量热检测系统 ,测定鲫鱼 (Carassiusauratus)肝脏线粒体体外代谢热及不同浓度联苯胺 (1.0 ,5 .0 ,10 .0 ,2.0 .0 ,4 0 .0mg/L)对其代谢活性的影响 .结果表明 ,线粒体体外代谢活性恢复期速率常数k1,活性衰竭期速率常数k2.以及最大放热功率Pmax均受到联苯胺的影响 ,且随着联苯胺浓度的升高。

关键词：联苯胺鲫鱼肝脏线粒体微量热代谢热代谢活性环境化学污染物毒性作用

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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Science china Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2. ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2.& WANG Shan4 1State Key Laboratory of environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085,{2. 2.3} of Chemistry, Tsinghua University, Beijing 100084,{2. 3Beijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing 100094,{2. 4School of Chemistry and Chemical engineering, Xianyang Normal college, Xianyang 712.00,{2. 1. State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2.2.-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-2.0 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

HYDRAULIC RESISTANCE OF SUBMERGED VEGETATION RELATED TO EFFECTIVE HEIGHT

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Journal of Hydrodynamics 2010年第2期22卷 265-273页

作者： WANG Pei-fang WANG Chao ZHU David Z. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Ministry of EducationCollege of Environmental Hohai University Nanjing 210098 China department of Civil and environmental {2. University of Alberta Edmonton Canada T6G 2W2

Submerged vegetation has a significant impact on water flow *** investigations include the impact through adding drag resistance and increasing bottom roughness coefficient,which cannot elucidate the characters of real submerged *** evaluate the effects of submerged vegetation on water currents at different velocities,a laboratory experiment was conducted using three kinds of *** effective heights of these vegetations on varying flow velocities were *** equation describing the relationship between the normalized resistance of the submerged plants and the Reynolds number based on the plant effective height was then established and used to calculate the hydraulic resistance parameters of submerged plants in different stages of growth.

关键词： hydraulic resistance submerged vegetation drag force effective height

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton(Gossypium hirsutum)

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Journal of Integrative Agriculture 2014年第10期13卷 2102-2112页

作者： NI Zhi-yong LI Bo Neumann M Peter Lü Meng FAN Ling Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences college of Agronomy Xinjiang Agricultural University Plant Physiology Laboratory Department of EnvironmentalWater and Agricultural EngineeringFaculty of Civil and Environmental EngineeringTechnion-Israel Institute of Technology

Two genes （GhC4H1 and GhC4H2. that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2.each contain open reading frames of 1 518 base pairs （bp） in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2.genomic DNA are 2.2.7 and 2.161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4HI and GhC4H2.promoters were isolated and found to contain many cis-elements （boxes P, L and AC-1 element） previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2.promoters in ovules and fibers tissues. GhC4H1 and GhC4H2.were also widely expressed in other cotton tissues. GhC4H2.expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2.in cotton fiber development.

关键词： cinnamate 4-hydroxylase Gossypium hirsutum promoter analysis phenylpropanoid pathway

REMOVAL OF PHOSPHATES FROM WATER BY PILLARED RECTORITE

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Chinese Journal of Reactive Polymers 2005年第Z1期 67-75页

作者： DU Dongyun 1,2.LI Dunshun3 YU Zhongshan2.1 Key Laboratory of Catalysis and Material Science of hubei Province, college of Chemistry and Material Science, South-Central University for Nationalities, Wuhan 430074,{2. 2.department of Chemistry and environmental engineering hubei Normal University, Huangshi 435002.{2. 3 Daye Non-ferrous Metals Company Limitted, Huangshi 435002.{2.

The presence of trace phosphates in treated wastewater from municipalities and industries is often responsible for eutrophication problems in lakes, rivers, and other water bodies. In this paper, we report the removal of PO43- from water by using a pillared rectorite that we synthesized recently. The results show that cross-linking can significantly increase the adsorbing capacity of Na-rectorite for phosphates. The pH, the concentrations of F-, NH4+ and COD are main factors, which affect the results for pillared rectorite to adsorb phosphates from water. The OH-, and F- ions decrease the capacity to adsorb phosphates, while the COD and NH4+ ions increase it.

关键词： Pillared Rectorite Removal of Phosphates Lake-Remediation.

A Formulation on Large Eddy Simulation

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Communications in Nonlinear Science and Numerical Simulation 1999年第4期4卷 3-5页

作者： K. R. RAJAGOP-AL* L. TAO* Guoqian CHEN**(*department of Mechanical engineering, Texas A&M University, {2. Station, Texas77843-312., USA)(**Center for environmental Sciences, Peking University, Beijing 100871, china) department of Mechanical engineering Texas A and M University College Station TX 77843-3123 United States Center for environmental Sciences Peking University Beijing 100871 China

IntroductionWithintheframeworkthattheNavier-StokesequationissupposedlyapplicabletoturbulentmotionsofaNewtonianfluid,largeeddysimulation(LES)isconsideredasacompromisebetweendirectnumericalsimulationofturbulence(DNS),wh... 详细信息

关键词： large eddy simulation turbulence modeling

Using lightning locating system based on time-of-arrival technique to study three-dimensional lightning discharge processes

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Science china Earth Sciences 2010年第4期53卷 591-602页

作者： ZHANG GuangShu1,WANG YanHui1,QIE XiuShu2.ZHANG Tong1,ZHAO YuXiang1,3,LI YaJun1 & CAO DongJie11 Laboratory for Climate Environment and Disasters of Western china,Cold and Arid Regions environmental and {2. Research Institute,Chinese Academy of Sciences,Lanzhou 730000,china 2.Laboratory for Middle Atmosphere and Global Environment Observation(LAGEO),Institute of Atmospheric Physics,Chinese Academy of Sciences,Beijing 10002.,china 3 department of Physics,Tianshui Normal college,Tianshui 741001,china 1. Laboratory for Climate Environment and Disasters of Western china Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 China2. Laboratory for Middle Atmosphere and Global Environment Observation (LAGEO) Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China3. Department of Physics Tianshui Normal College Tianshui 741001 China

A time-of-arrival(TOA) system based on GPS technology for locating VHF radiation sources from lightning has been developed and used in observation sites in the northern Shandong Province,*** 3D images of the lightning progression have been obtained successfully for the first time in *** 3D-channel evolutions of typical negative CG,positive CG and IC lightning flashes have been discussed together with the data of fast electric field *** was found that significant differences existed between the negative and positive CG lightning flashes in terms of the initiation and propagation of the radiation *** preliminary breakdown of a negative CG lightning flash propagated at a speed about 5.2.104 m/*** stepped leader of negative CG lightning flashes was trigged by negative initial ***,it propagated downward at a speed of 1.3×105 m/*** initial process of the positive CG lightning flashes was also a propagation process of negative *** streamers propagated dominantly horizontally in the positive charge region and accumulated positive charges at the origin of the lightning,and as a consequence,initiated downward positive streamers.A new type of lightning discharge that was triggered by a narrow bipolar pulse(NBP) is discussed in this *** NBP was originated at altitude of about 10.5 km in the upper positive charge *** a distinct difference from normal IC flash,its channels extended horizontally all around and produced a lot of radiation *** source power of the NBP could approach 16.7 kW,which is much greater than that of normal lightning discharge ranging between 100 mW and 500 *** 3D propagation of this new type of lightning discharge was observed and obtained for the first time in *** possible initiation mechanism of this new type of light-ning is discussed here.

关键词： time-of-arrival(TOA) technique three dimensional lightning locating system discharge process narrow bipolar pulse new-type of IC lightning

Particle monitoring techniques for water treatment applications

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Journal of environmental Sciences 1999年第3期11卷 328-333页

作者： John Gregory department of Civil and environmental {2., University college London, Gower St, London WC1E 6BT, UK University college London 英国

A simple optical technique based on fluctuations in light transmitted through flowing suspensions has proved useful in two distinct water treatment applications. The first of these is the monitoring and control of coagulation/flocculation processes, where information on optimum coagulant dosages as well as on the dynamics of floc formation can be derived. The method is suitable for a very wide range of particle concentrations, up to levels found in highly turbid river waters. The second application is as a very sensitive monitor of particles in filtered water. Such monitoring can help to ensure the effective removal of pathogens such as Cryptosporidium from drinking water. Brief accounts of these techniques are given, together with some examples of their use.

关键词： Cryptosporidium coagulation flocculation fluctuations monitoring particles water treatment.

掺杂Eu^(3+)离子的钨酸锂的固相合成及其发光特性

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稀土 2004年第1期25卷 17-19页

作者：杨水金孙聚堂湖北师范学院化学与环境工程系湖北黄石435002 武汉大学化学与分子科学学院湖北武汉430072

采用固相反应法合成了掺杂Eu3+的Li2.O4材料,并通过X射线粉末衍射对其结构进行了表征,掺杂Eu3+的Li2.O4的晶体结构属四方晶系,晶胞参数a=14.2.80 ,c=9.5863 ,属R3(No.148)空间群,测定了其激发光谱和发射光谱,探讨了掺杂Eu3+的Li2.O4的... 详细信息

采用固相反应法合成了掺杂Eu3+的Li2.O4材料,并通过X射线粉末衍射对其结构进行了表征,掺杂Eu3+的Li2.O4的晶体结构属四方晶系,晶胞参数a=14.2.80 ,c=9.5863 ,属R3(No.148)空间群,测定了其激发光谱和发射光谱,探讨了掺杂Eu3+的Li2.O4的发光特性。

关键词： Eu^3+ Li2.O4 合成发光