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Reconstruction of the most recent common ancestor sequences of SARS-Cov S gene and detection of adaptive evolution in the spike protein

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Chinese Science Bulletin 2004年第12期49卷 1311-1313页

作者： ZHANG Yuan1, ZHENG Nan2.3, HAO Pei4,5 & ZHONG Yang6 1. Laboratory of Systematic and Evolutionary Botany, Institute of Bot- any, Chinese Academy of sciences, beijing 100093,{2. 2. college of life sciences, beijing normal university, beijing 100875,{2. 3. Institute of Viral Disease Control and Prevention, Chinese CDC, beijing 100052.{2. 4. Bioinformation Center, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, Shanghai 2.0031,{2. 5. Shanghai Center for Bioinformation Technology, Shanghai 2.02.5,{2. 6. School of life sciences, Fudan university, Shanghai 2.0433,{2. 1. Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences 100093 Beijing China 2. college of life sciences Beijing Normal University 100875 Beijing China 3. Institute of Viral Disease Control and Prevention Chinese CDC 100052 Beijing China 4. Bioinformation Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 200031 Shanghai China 5. Shanghai Center for Bioinformation Technology 200235 Shanghai China 6. School of life sciences Fudan University 200433 Shanghai China

The genome oiganization and expression strategy of severe acute respiratory syndrome coronavirus (SARS-CoV) have been described extensively. As a structural glycoprotein on the virion surface, the spike protein is responsible for binding to host cellular receptors and for the fusion between the viral envelope and the cellular membrane. It also induces neutralizing antibodies in the host and mediates cellular immunity. Previous studies suggested that amino acid replacements in the spike protein coulddramatically alter the pathogenesis and virulence of some coronaviruses. It is therefore reasonable to test the hypothesis that radical amino acid replacements in the spike protein, favored by environmental selective pressure during the process of SARS-CoV interspecific transmission, might make this pathogen adapt to a new host, In this study, we investigated a total of 108 complete sequences of the SARS-CoV S gene from Gen-Bank (until March 2., 2.04). After omission of those records containing frame-shift mutations or low quality sequences, e.g. ZJ01, and selection of one sequence for identical records, an alignment of 42.sequences was obtained using the program Clustal-X. Then, we reconstructed the most recent common ancestor (MRCA) sequences of theSARS-CoV S gene and detected the adaptive evolution in the spike protein.

关键词： SARS冠状病毒自适应进化基因表达糖蛋白分子免疫学波峰病毒

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The effects of proteasome inhibitor lactacystin on mouse oocyte meiosis and first cleavage

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Science china(life {2.) 2005年第3期48卷 287-294页

作者： TAN Xin 1,3 , PENG An 2., WANG Yongchao 2.& TANG Zuoqing 3 1. School of life sciences and Technology, {2. Institute of Technology, {2. 100081, china 2. college of life sciences, beijing normal university, beijing 100875, china 3. college of Basic Medicine, Capital university of Medical sciences, {2. 100054, china 1. School of life sciences and Technology Beijing Institute of Technology 100081 Beijing China 2. college of Basic Medicine Capital University of Medical Sciences 100054 Beijing China 3. college of life {2. Beijing Normal University 100875 Beijing China

In order to study the effects of ubiquitin-proteasome pathway (UPP) on mouse oo- cyte meiosis and cleavage, oocytes undergoing maturation and parthenogenetic activation and 1-cell embryos were treated with lactacystin, a specific inhibitor of proteasome. The results indi- cated that the rate of GVBD was not influenced by the treatment, but polar body extrusion, par- thenogenesis and first cleavage were inhibited. Immunofluorescent staining using anti β-tubulin antibody indicated that the continuous treatment of lactacystin from GV stage disorganized microtubules and spindle assembly. When metaphase stage oocytes were treated with the drug, the already formed spindle structure was not affected , but the oocytes were arrested at meta- phases. The 1-cell embryos were arrested at interphase or metaphase of first mitosis when they were incubated in the drug. Proteasome regulatory subunit PA700 was located in the spindle region, as indicated by immunofluorescence. These results suggest that UPP has effects on the process of oocyte meiosis and early cleavage in many aspects, including normal organization of spindle at prophase and segregation of chromosomes at anaphase for normal meiosis.

关键词： proteasome, oocyte, meiosis, cleavage.

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Cloning and analysis of a Y-tocopherol methyltransf erase gene from Brassica oleracea and the function of its recombinant protein

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Progress in Natural Science:Materials International 2003年第7期 33-38页

作者： OUYANG Qing, FAN Chuntao, SUN Hui, ZHANG Yuman, BAI Shuangyi, CAI Wenqi (1. Institute of Microbiology, Chinese Academy of sciences, beijing 100080,{2. 2. college of life sciences, beijing normal university, beijing 100875,{2. 3. Department of Landscape Architecture,{2. Agricultural university, beijing 100094,{2.)

The full-length cDNA (BoTMT) of γ-TMT is obtained from Brassica oleracea by 3'- and 5'-RACE methods. The 12.5 bp cDNA contains an open reading frame of 1044 bp, which encodes a protein of 347 amino acids with a predicted chloroplast transit peptide and two S-adenosylmethionine (SAM)-binding domains. Sequence analysis shows that the deduced protein shares 41. 8% -86. 5% similarity to known γ-TMTs. Semi-quantitative RT-PCR reveals that BoTMT is expressed preferentially in flowers and leaves of B. oleracea . The recombinant γ-TMT protein is obtained by cloning its encoding region into the prokaryotic expression vector pET30a. The protein expressed in E. coli accounts for 2. % of total bacterial protein. The enzyme activity assay indicates that the recombinant protein has relatively high activity to convert γ-tocopherol to α-tocopherol in vitro.

关键词： : Brassica oleracea gamma-tocopherol methyltransferase gene and expression enzyme activity assay tocopherol.

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四川省老君山自然保护区棕噪鹛和丽色噪鹛繁殖巢的记述

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四川动物 2010年第3期29卷 488-488页

作者：付义强张正旺陈本平凌征文乐山师范学院化学与生命科学学院四川乐山614000 北京师范大学生命科学学院北京100875 四川老君山自然保护区四川屏山645350

棕噪鹛Garrulax poecilorhynchus是中国特有种鸟类,主要分布在我国长江以南的一些地区的山地常绿阔叶林中,数量稀少(雷富民,卢汰春,2.06).丽色噪鹛Garrulax formosus分布于我国中部至越南北部,国内罕见于四川中部及西部、云南北部及广西... 详细信息

棕噪鹛Garrulax poecilorhynchus是中国特有种鸟类,主要分布在我国长江以南的一些地区的山地常绿阔叶林中,数量稀少(雷富民,卢汰春,2.06).丽色噪鹛Garrulax formosus分布于我国中部至越南北部,国内罕见于四川中部及西部、云南北部及广西,常结群栖息于海拔900～3000 m的山区常绿林、次生林及竹林的地面或近地面处(约翰·马敬能等,2.00).目前关于这2.噪鹛的野外繁殖资料非常匮乏.

关键词：棕噪鹛丽色噪鹛繁殖巢老君山

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The toxicity of Cu^(2.) to Microcystis aeruginosa using dielectric spectroscopy

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Science china Chemistry 2010年第3期53卷 631-636页

作者： YU YanLi1,BAI Wei1,ZHAO KongShuang1& ZHOU YunLong2.1college of Chemistry,beijing {2. university,beijing 100875,china 2.0} of life Science,beijing {2. university,beijing 100875,china 1. college of Chemistry Beijing Normal University Beijing 100875 China2. College of Life Science Beijing Normal University Beijing 100875 China

Dielectric spectroscopy(DS) was utilized to investigate the toxicity of the Cu2. solution to Microcystis *** was found that 0.04 mM Cu2. had no effect on dielectric increment,whereas the Cu2. concentrations above 0.1 mM decreased the value of Δε/Δε *** toxic effect of Cu2. on *** cells was time-dependent and *** was a threshold concentration between 0.04 mM and 0.1 mM,which affected the cell membrane,suggesting that Cu2. at the concentrations higher than 0.1 mM caused serious plasmalemma damage to *** *** addition of 1 mM Ca2. or Mg2. did not protect *** cells from 1 mM Cu2. *** study demonstrates that DS monitors cell biomass facilitating observation of the toxic effects of metal ions.

关键词： Microcystis aeruginosa dielectric spectroscopy dielectric increment cupric ion toxicity

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Cu(Ⅱ) Potentiation of Alzheimer Aβ1-40 Cytotoxicity and Transition on its Secondary Structure

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 765-772页

作者： Xue-Ling DAI~1 Ya-Xuan SUN~2.Zhao-Feng JIANG~2. 1 college of life Science,Capital {2. university,beijing 100037,china 2.college of Applied sciences and Humanities,beijing Union university,beijing 100083,china college of life Science Capital Normal UniversityBeijing 100037 China college of Applied sciences and Humanities Beijing Union UniversityBeijing 100083 China

Mounting evidence has shown that dyshomeostasis of the redox-active biometals such as Cuand Fe can lead to oxidative stress,which plays a key role in the neuropathology of Alzheimer's disease（AD）.Here we demonstrate that with the formation of Cu（Ⅱ）·Aβ1-40 complexes,copper markedly potentiatesthe neurotoxicity exhibited by β-amyloid peptide （Aβ）.A greater amount of hydrogen peroxide was releasedwhen Cu（Ⅱ）·Aβ1-40 complexes was added to the xanthine oxidase/xanthine system detected by potassiumiodide *** bound to Aβ1-40 was observed by electron paramagnetic resonance（EPR） *** dichroism （CD） studies indicated that copper chelation could cause a structuraltransition of Aβ.The addition of copper to Aβ introduced an increase on β-sheet as well as α-helix,whichmay be responsible for the aggregation of Aβ.We hypothesized that Aβ aggregation induced by copper maybe responsible for local injury in *** interaction between Cu2.and Aβ also provides a possible mechanismfor the enrichment of metal ions in amyloid plaques in the AD brain.

关键词： Alzheimer’s disease Cu(Ⅱ)·Aβ1-40 complex neuron injury Aβ secondary structure

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Development and application of ergodicity model with FRCM and FLAR for hydrological process

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Science china(Technological sciences) 2009年第2期52卷 379-386页

作者： WANG HongRui1, FENG QiLei2. LIN Xin3 & ZENG WenYi4 1 college of Water Science, Key Laboratory for Water and Sediment sciences of the Ministry of Education,{2., beijing 100875, china 2.School of Science, beijing Institute of Education, beijing 100011, china 3 School of Mathematical sciences,{2., beijing 100875, china 4 college of Information Science and Technology,{2., beijing 100875, china 1. college of Water Science Key Laboratory for Water and Sediment Sciences of the Ministry of Education Beijing Normal University Beijing 100875 China 2. School of Science Beijing Institute of Education Beijing 100011 China 3. School of Mathematical sciences Beijing Normal University Beijing 100875 China 4. college of Information Science and Technology Beijing Normal University Beijing 100875 China

In this paper, a new ergodic property analysis model of hydrological process is proposed based on fuzzy-rough c-means clustering (FRCM), autocorrelogram, and fuzzy least absolute regression (FLAR). A precipitation time series (1951―2.04) from Shanghai Hydrology Station is then analyzed with the model. The results show that the precipitation time series of April, May, June, and September has er-godic property. We conclude that in the long run, the precipitation of April, May, June, and September will not keep decreasing; it will converge to its mean value in some period.

关键词： hydrological process ergodic autocorrelogram fuzzy-rough c-means clustering fuzzy least absolute regression

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MAPK signal pathways in the regulation of cell proliferation in mammalian cells

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Cell Research 2002年第1期12卷 9-18页

作者： WEI ZHANG, Hui Tu LIU The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, college of life sciences, beijing normal university, beijing 100875, china 1The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Sciences Beijing Normal University Beijing 100875 China 1The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Sciences Beijing Normal University Beijing 100875 China

MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation.

关键词： MAPK ER.K JNK/SPAK p38 cell proliferation cross-talk.

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Effects of chronic exposure to nonylphenol on locomotor activity and social behavior in zebrafish(Danio rerio)

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Journal of Environmental sciences 2010年第9期22卷 1435-1440页

作者： Jigang Xia,Cuijuan Niu ,Xuejiao Pei Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering,college of life {2.,beijing normal university,100875 beijing,china. [a] Ministry of Education Key Laboratory of Biodiversity Science Ecological Engineering College of Life Sciences Beijing Normal University 100875 Beijing China

Nonylphenol （NP）, a xenoestrogen ubiquitously found in aquatic ecosystems, is of high environmental concern. The present work assessed the effects of exposure to NP on locomotor activity and social behavior in male and female zebrafish （Danio rerio）, to evaluate the possible hazard of NP to fish behavior and to pursue a potential biomarker of NP contamination. Fish were randomly divided into six groups. Five groups were receiving 0.1, 1, 10, 50 and 100 μg/L nominal concentrations of NP for 60 days, respectively. A sixth control group was given the same treatment as the other five groups, but no NP. Locomotor activity, aggressive behavior, group preference and leaving shoal were examined. NP exposure showed marked influence on locomotor activity of the male zebrafish, whereas that of the female was not significantly affected by NP. Aggressive behavior and group preference were significantly affected by NP exposure in both male and female groups. Locomotor activity and aggressive behavior of the male and group preference of both male and female zebrafish were clearly inhibited at 100 μg/L NP. No significant alteration in leaving shoal was observed under NP exposure. The results suggested that changes in locomotor activity and aggressive behavior of the male and group preference of both male and female may be used as an ecologically relevant integrative biomarker of NP contamination.

关键词： chronic toxicity nonylphenol biomarker zebrafish

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A New Potent Inhibitor of Thrombin from the Leech Haemendipsa Yanyuanensis

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Journal of Shanghai university(English Edition) 2001年第1期5卷 86-88页

作者： ZHAO Rong le college of life {2., beijing normal university, beijing 100875, china college of life {2. Beijing Normal University Beijing China

Two components of anticoagulant protein were isolated from the leech Haemendipsa yanyuanensis by heparin agarose affinity chromatography and ultracentrifugation. The determination of anticoagulant activity and characterization analysis of the protein using the method of chromogenic substrate indicates that the anticoagulant protein is thrombin specific but not factor Xa specific. The results lay a foundation for the research of the anticoagulant mechanism and application of anticoagulant protein from H. yanyuanensis.

关键词： Heamendipsa yanyuanensis anticoagulant protein thrombin factor Xa

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