检索结果-南通市图书馆

CRISPR／Cas9在丝状真菌基因组编辑中的应用

2.07中国植物生理学会全国学术会议

作者： Jian-Hua Li~1 Yin-Qian Liu~1 Pin Lu~2.Hai-Fei Lin~1 Yang Bai~1 Xue-Chen Wang~3.Yu-Ling Chen~(1*) (1 college of Life Science,Hebei Normal university,Shijiazhuang 050016,{2.) (2.School of Basic Medical sciences,Hebei Medical university,Shijiazhuang 050017,{2.) (3.National Laboratory of Plant Physiology and Biochemistry,college of Biological sciences,{2. Agricultural university,beijing 100094,{2.)

Extracellular calmodulin(ExtCaM)regulates stomatal movement by eliciting a cascade of intracellular signaling events including heterotrimeric G protein,HO and ***,the ExtCaM-mediated guard cell signaling pathway remains poorly *** this report,we show that Arabidopsis(Arabidopsis thaliana)nitric oxide synthase 1(AtNOS1)-mediated nitric oxide(NO)generation plays a crucial role in

关键词： Extracellular calmodulin stomatal movements G protein H`2.`2 nitric oxide

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

遗传 2017年第5期39卷 355-367页

作者：李红花刘钢中国科学院微生物研究所真菌学国家重点实验室北京100101 中国科学院大学北京100049

丝状真菌(filamentous fungi)通常指那些菌丝体较发达且不产生大型肉质子实体结构的真核微生物。丝状真菌不仅在自然界物质循环中发挥着重要作用,还与人类健康和工农业生产有着紧密的联系。然而,对丝状真菌进行遗传操作相对困难,极大地... 详细信息

丝状真菌(filamentous fungi)通常指那些菌丝体较发达且不产生大型肉质子实体结构的真核微生物。丝状真菌不仅在自然界物质循环中发挥着重要作用,还与人类健康和工农业生产有着紧密的联系。然而,对丝状真菌进行遗传操作相对困难,极大地妨碍了丝状真菌的遗传学研究。成簇的规律间隔的短回文重复序列及其相关系统(clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9,CRISPR/Cas9)是近年来发现的一种存在于细菌和古菌中保守的获得性免疫防御机制。最近,CRISPR/Cas9被开发成为了一种方便灵活的基因组编辑技术。目前,该技术已经广泛应用在不同物种的基因组编辑中。本文概述了CRISPR/Cas9在丝状真菌基因组编辑中的应用进展,旨在为开展该领域的研究工作提供参考。

关键词：丝状真菌 CRISPR/Cas9 基因组编辑应用

SYMPATHETIC PARTIAL AND FULL FILAMENT ERUPTIONS OBSERVED IN ONE SOLAR BREAKOUT EVENT

在线全文

学校读者我要写书评

暂无评论

SYMPATHETIC PARTIAL AND FULL FILAMENT ERUPTIONS OBSERVED IN ...

Unscented Kalman filtering in the additive noise case

强化科技基础推进气象现代化——第2.届中国气象学会年会

作者： Yuandeng Shen1,2.3.Yu Liu1,3.and Jiangtao Su3.1 Yunnan Astronomical Observatory,Chinese Academy of sciences,Kunming 650011,china 2.Graduate {2. of Chinese Academy of sciences,beijing 100049,china 3.Key Laboratory of Solar Activity,National Astronomical Observatories,Chinese Academy of Science,beijing 100012.china

We report two sympathetic solar eruptions including a partial and a full flux rope eruption in a quadrupolar magnetic region where a large and a small filament resided above the middle and the east neutral lines,*** large filament first rose slowly at a speed of 8 km s-1 for 2. minutes;it then accelerated to 102.km ***,this filament erupted successfully and caused a coronal mass *** the slow rising phase,various evidence for breakout-like external reconnection has been identified at high and low temperature *** eruption of the small filament started around the end of the large filament’s slow *** filament erupted partially,and no associated coronal mass ejection could be *** on a potential field extrapolation,we find that the topology of the three-dimensional coronal field above the source region is composed of three low-lying lobes and a large overlying flux system,and a null point located between the middle lobe and the overlying antiparallel flux *** propose a possible mechanism within the framework of the magnetic breakout model to interpret the sympathetic filament eruptions,in which the magnetic implosion mechanism is thought to be a possible link between the sympathetic eruptions,and the external reconnection at the null point transfers field lines from the middle lobe to the lateral lobes and thereby leads to the full(partial) eruption of the observed large(small) *** possible mechanisms are also discussed *** conclude that the structural properties of coronal fields are important for producing sympathetic eruptions.

关键词： Sun:activity-Sun:coronal mass ejections(CMEs)-Sun:filaments prominences-Sun:flares-Sun:magnetic topology

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science china(Technological sciences) 2010年第11期53卷 929-941页

作者： LIU Ye1,YU AnXi1,ZHU JuBo2.& LIANG DianNong1 1 college of Electronic Science and Engineering,National university of Defense Technology,Changsha 410073.{2. 2.Science college,National university of Defense Technology,Changsha 410073.{2. college of Electronic Science and Engineering National University of Defense Technology Science college National University of Defense Technology

The unscented Kalman filter(UKF) has four implementations in the additive noise case,according to whether the state is augmented with noise vectors and whether a new set of sigma points is redrawn from the predicted state(which is so-called resampling) for the observation *** paper concerns the differences of performances for those implementations,such as accuracy,adaptability,computational complexity,*** conditionally equivalent relationships between the augmented and non-augmented unscented transforms(UTs) are proved for several sampling strategies that are commonly ***,we find that the augmented and non-augmented UKFs have the same filter results with the additive measurement noise,but only have the same state predictions with the additive process *** is not believed to be necessary in some ***,we find out that resampling can be helpful for an adaptive Kalman *** will improve the convergence and accuracy of the filter when the large scale state modeling bias or unknown maneuvers ***,some universal designing principles for a practical UKF are given as follows:1) for the additive observation noise case,it's better to use the non-augmented UKF;2. for the additive process noise case,when the small state modeling bias or maneuvers are involved,the non-resampling algorithms with state whether augmented or not are candidates for filters;3. the resampling and non-augmented algorithm is the only choice while the large state modeling bias or maneuvers are latent.

关键词： unscented Kalman filter additive noise unscented transformation augmented redraw sigma points adaptive

同方期刊数据库

Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism

在线全文

学校读者我要写书评

暂无评论

Science china(Life sciences) 2009年第11期52卷 1073-1080页

作者： ZHOU CanPing1,2. WANG JiaBo1, ZHANG XueRu1,2. ZHAO YanLing1, XIA XinHua2. ZHAO HaiPing1, REN YongShen1 & XIAO XiaoHe1 1 {3. Military Institute of Chinese Meteria Medica, 3.2.Military Hospital of {3., {2. 10003., {3. 2.college of Pharmaceutical sciences, Hunan university of Chinese Medicine, Changsha 4102.8, {3. china Military Institute of Chinese Meteria Medica 302 Military Hospital of China Beijing China

The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (P<0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P<0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism.

关键词： "Cold" and "Hot" natures of Chinese medicine evaluation temperature tropism ethology drug process Coptis chinensis Franch

Analysis and experimentation of key technologies in service-oriented optical internet

在线全文

学校读者我要写书评

暂无评论

Science china(Information sciences) 2011年第2期54卷 215-226页

作者： JI YueFeng, REN DanPing, LI Hui, LIU XueJun & WANG ZhengZhong Key Laboratory of Information Photonics and Optical Communications, Ministry of Education, beijing university of Posts and Telecommunications, beijing 100876, {3. Key Laboratory of Information Photonics and Optical Communications Ministry of Education Beijing University of Posts and Telecommunications

To satisfy diverse bandwidth-intensive applications envisioned in the internet of the future, research on a distributed optical internet, enabling efficient utilization of geographically distributed resources, has become attractive. The deployment of such a network, however, has its own technical challenges, including network architecture, resource management, and high-speed transmission. To address these issues, a service-oriented-architecture-based distributed optical internet architecture is proposed. Thereafter, key technologies of an optical internet, such as optical multicasting, distributed resource control, and high-speed transmission, are analyzed and investigated. The proposed schemes are verified through experimentation and simulation.

关键词： optical internet service-oriented distributed optical networks optical multicasting optical transmission

LAWS OF THE ITERAFED LOGARITHM FOR TRIMMED SUM

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Systems Science and Mathematical sciences 1998年第1期11卷 39-46页

作者： PENG Liang(Deperttnent of Probebility and Statistics, Pebng university, beijing 10087l, {2.)

In this paPer we shall give a theorem to explain the conjecture of P. S. ***[1].

关键词： Law of the iterated logarithm trixnmed sum.

Establishment and Utilization of a Tetracycline-controlled Inducible RNA Interfering System to Repress Gene Expression in Chronic Myelogenous Leukemia Cells

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第12期37卷 851-856页

作者： Fan YANG Yun ZHANG Ying-Li CAO Shu-Hui WANG Li LIU Department of Microbiology and Etiology,Institute of Basic Medical .sic Medical sciences. Chinese Academy q/’Medical sciences and Peking Union Medical {2..{3. 100005.china DepartmentofMicrobiologyandEtiology InstituteofBasicMedicalSciencesChineseAcademyofMedicalSciencesandPekin

RNA interference (RNAi),a posttranscriptional gene silencing process mediated by small double-stranded RNA specifically complementary to the targeted transcript,has been used extensively in the developmentof novel therapeutic approaches against various human diseases including chronic myelogenous leukemia (CML).*** report the successful construction of a tetracycline-controlled siRNA in CML cell line K562.A K562.ell line stably expressing the reverse tetracycline-controlled transactivator (rtTA) was constructed.A tetracy-cline responsive element(TRE) was integrated into the RNA polymerase Ⅲ promoter region of pBS/U6 thatwas used to drive specific siRNA to target the novel cytokine receptor-like factor 3.(CRLF3. gene,The resultsshow that rtTA was able to recognize the TRE to prevent siRNA-mediated exogenous and endogenous CRLF3.ene ***,CRLF3.siRNA mediated gene repression could be induced in a dose-dependentmanner in the presence of ***,the inducible siRNAi system in K562.cells might be useful for thestudy of RNAi-mediated therapeutic approaches against CML.

关键词： inducible siRNA tetracycline reverse tetracycline-controlled transactivator U6 promoter CRLF3

ALTERATION IN CHROMATIN CONFORMATION OF PROLIFERATING AND DIFFERENTIATING ENZYME GENES IN NORMAL MOUSE LIVER AND ASCITES HEPATOMA CELLS AND ITS RELATION TO THE GENE EXPRESSION OF ENZYMES

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science in china,Ser.B 1988年第11期31卷 1319-1324页

作者：李士谔马恩陵吴石君 Faculty of Basic Medicine Beijing Union Medical University Institute of Basic Medical sciences Chinese Academy of Medical Sciences BeijingFaculty of Basic Medicine Beijing Union Medical University

Nuclei from the normal mouse liver were partially digested with micrococcal nuclease, followed by DNA extraction, agarose gel clectrophoresis and dot blot hybridization with 3.0}-labeled cDNA probes of CPS1 and ACT complex, It was clearly shown that the CPS1 genes were distributed on the monomer, dimer. and trimer of nucleosomes, while the genes coding for ACT complex were distributed on the condensed oligonucleosomes. An opposite manner of distribution of CPS1 and ACT complex genes was, however, noted in the case of ascites hepatoma cells, in which the specific activity of ACT was 13.times higher than that in the normal liver, while that of CPS1 was remarkably reduced. Similar patterns of change in mRNA level of CPS1 and ACT complex were observed in the normal mouse liver and ascites hepatoma cells, indicating a close relationship between chromatin structure and gene expression of these enzymes.

关键词： gene expression chromatin conformation CPS1 ACT hepatoma.

The noble gas isotope geochemical composition of chert at the bottom of Cambrian in Tarim Basin,china

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china Earth sciences 2009年第S1期52卷 115-119页

作者： SUN ShengLi1,CHEN JianFa2.3.ZHENG JianJing4 & LIU WenHui4 1 Monitoring Center for Marine Resource and Environment,Guangdong Ocean university,Zhanjiang 52.088,{3. 2.Department of {2.chemistry,Changjiang university,Jingzhou 43.02.,{3. 3.college of Resource and Information,Petroleum university,beijing 102.49,{3. 4 Lanzhou Institute of {2.logy,Chinese Academy of sciences,Lanzhou 73.000,{3.

In the Tarim Basin,black shale series at the bottom of Cambrian is one of the important marine facies hydrocarbon source rocks. This research focuses on the analysis of the isotope of noble gas of 11 cherts. The R/Ra ratio of chert in the Keping area is 0.03.0}0.3.9,and 40Ar/3.Ar is 3.8―43.. In Quruqtagh the R/Ra ratio is 0.44―10.2.,and 40Ar/3.Ar is 3.0―765. The R/Ra ratio of chert increases with 40Ar/3.Ar from the west to the east accordingly. They have evolved from the crust source area to the mantle source area in a direct proportion. Surplus argon 40ArE in chert is in direct proportion to the R/Ra ratio,indicating that it has the same origin of excess argon as in fluid and mantle source helium. Comparison of the R/Ra ratios between the west and the east shows that the chert in the eastern part formed from the activity system of the bottom hydrothermal venting driven by the mantle source,where the material and energy of crust and mantle had a strong interaction in exchange;whereas in the western part,chert deposited from the floating of hydrothermal plume undersea bottom,which is far away from the centre of activities of the hydrothermal fluid of ocean bottom. In addition,from noble gas isotope composition of chert,it is suggested that the ocean anoxia incident happened at the black shale of the Cambrian bottom probably because of the large-scaled ocean volcanoes and the followinghydrothermal activities.

关键词： Tarim Basin Cambrian chert rare gas isotope