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稳定膜透水率的新方法-恒流控制

水处理技术 2001年第4期27卷 214-216页

作者：彭跃莲刘忠洲北京工业大学环境与能源工程学院北京100022 中国科学院生态环境研究中心北京100085

本文从理论上分析了减轻膜污染、提高膜产水量的有效方法之一是降低膜的平均操作压力 ,并在此基础上提出了恒流控制的操作方式 ,即保证在一个周期内膜组件的透过液流量恒定 ,以及实现恒流控制的三种方法。本研究还通过实验在分置式膜生... 详细信息

本文从理论上分析了减轻膜污染、提高膜产水量的有效方法之一是降低膜的平均操作压力 ,并在此基础上提出了恒流控制的操作方式 ,即保证在一个周期内膜组件的透过液流量恒定 ,以及实现恒流控制的三种方法。本研究还通过实验在分置式膜生物反应器中比较了相同条件下恒流控制与传统的恒压控制时膜的产水量 ,结果表明前者比后者提高了 1 2..6 %。

关键词：膜污染恒流控制膜生物反应器膜透水率聚砜膜过滤

Proteomic analysis and dynamic changes of proteins in cultured spinal cord astrocytes after injured in vitro

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Proteomic analysis and dynamic changes of proteins in cultur...

中国蛋白质组学第三届学术大会

作者： Gang Pan~1, Qingxue Ding~1, Zhe Wu, Qiuxia Lin~1, Haiping Que~1, Hongxia Wang~2. Kaihua Wei~2. Shuqian Jing~(1,3), Shaojun Liu~1 1 Department of Neurobiology, beijing Institute of Basic Medical Science, china 2.Chinese National {2. of biomedical Analysis, china 3 Department of Clinical Immunology, Amgen Inc., One Amgen {2. Drive, Thousand Oaks, CA 9132., USA

Astrocyte,predominant glia cell in the spinal cord, is one of the earliest cells responding to CNS injury. To seek the response and the dynamic changes in proteins expression of astrocytes after injury, 2. electrophoresis and proteome protocols were employed to visualize and identify the different expression proteins. We first isolated primary astrocytes from the new-born rat spinal cord. The pure astrocytes, with over 97% purity identified with immunofluorescence were applied for injury experiments.A newly design mechanical injury model was used to injure the cultured astrocytes. At 2., 72.and 12. hours after injury, the expressed proteins were isolated and 2.E protocols were processed. Totally 1785, 1806, 1809 and 1841 protein spots were detected respectively in control, 2. h, 72.h and 12. h via ImageMaster software, and 50 different protein spots were detected by 2.E gels. They were divided into three categories of dynamic changes: 1. 15 spots, can only be found at a special stage after injury;2. 6 spots, vanish only at a special stage after injury;3. 2. spots, up-regulated or down-regulated at different periods after injury. These proteins were further identified with MALDI-TOF and analyzed. The results were identified with RT-PCR and Western blot. These differently displayed protein spots could be sorted as:(i) metabolic, e.g. dimethylarginine dimethylaminohydrolase 1 (DDAH1) and NAD-independent Isocitrate dehydrogenase (NAD-IDH), (ii) cyto-skeletal and intracellular protein transport, e.g., beta-actin and vimentin, (iii) molecular chaperoning, e. g., prohibitin and Valosin-containing protein (VCP);(iv) others. The results of this study indicate that these proteins may be involved in the process of SCI.

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Distributed Output Regulation of Uncertain and Switching Multi-Agent Systems via Internal Model Design

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Distributed Output Regulation of Uncertain and Switching Mul...

2.11 Chinese Control and Decision Conference（CCDC）

作者： Xiaoli Wang is with School of Information Science and engineering,Harbin Institute of Technology at Weihai,Weihai 2.42.9,Shandong,china Yiguang Hong is with the Key Laboratory of Systems and Control,Academy of Mathematics and Systems Science,Chinese Academy of Sciences,{2. 100190,china Zhong-Ping Jiang is with Department of Electrical and Computer engineering,polytechnic Institute of New York univer.ity,Six Metrotech center,Brooklyn,NY 112.1,USA

In this paper,the distributed robust output regulation problem of linear multi-agent systems （MAS） is *** driving force from the active leaders or the environmental disturbances is formulated as an input of an exogenous system （or exosystem） of the considered multi-agent networks.A systematic distributed design approach is proposed to handle output regulation via dynamic output feedback with the help of canonical internal model （IM）.With common solutions of regulator equations and Lyapunov functions,the distributed robust output regulation with switching interconnection topology is designed to achieve collective aims.

关键词： Output regulation Multi-agent Systems Internal Model Switching Topology

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深化对我国所有制基本结构认识的几个问题

深圳职业技术学院学报 2003年第1期2卷 38-44页

作者：鲁从明中央党校经济学教研部北京100091

公有制为主体的实质是公有制在社会经济中居于支配地位,但这种支配地位主要取决于公有制的质量和功能,而不是单纯或主要表现为经济总量的过半。非公有制经济还有进一步发展的必要性与可能性,并且还有很大的发展空间,甚至有可能在一定... 详细信息

公有制为主体的实质是公有制在社会经济中居于支配地位,但这种支配地位主要取决于公有制的质量和功能,而不是单纯或主要表现为经济总量的过半。非公有制经济还有进一步发展的必要性与可能性,并且还有很大的发展空间,甚至有可能在一定程度上超过公有制经济的总量。私营企业主作为一个社会群体和阶层,属于社会主义建设者。私营经济是社会主义市场经济中的重要而且充满活力的组成部分。从有利于调动投资者的积极性,发展社会主义市场经济的目的出发,应将我国现阶段凭借资本私有权获得的利润定为合法的非劳动收入。

关键词：所有制结构公有制为主体非公有制经济合法的非劳动收入

蛋白质组研究中肽质量指纹谱鉴定方法的建立及应用

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生物化学与生物物理学报 2000年第4期32卷 373-378页

作者：王京兰万晶宏罗凌熊少祥王光辉贺福初钱小红军事医学科学院国家生物医学分析中心!北京100850 军事医学科学院放射医学研究所!北京100850 中国科学院化学所国家质谱中心!北京100080 军事医学?

建立了用肽质量指纹谱和数据库检索方法鉴定凝胶电泳分离蛋白质的方法。用标准蛋白质对胶上蛋白质原位酶切制备肽谱的方法进行了讨论。分析了实际细胞蛋白质样品 ,获得了双向电泳分离的人肺癌细胞蛋白质谱中三个蛋白质点的肽指纹谱 ,并... 详细信息

建立了用肽质量指纹谱和数据库检索方法鉴定凝胶电泳分离蛋白质的方法。用标准蛋白质对胶上蛋白质原位酶切制备肽谱的方法进行了讨论。分析了实际细胞蛋白质样品 ,获得了双向电泳分离的人肺癌细胞蛋白质谱中三个蛋白质点的肽指纹谱 ,并通过数据库检索分别鉴定为甘油醛 3 磷酸脱氢酶 2.遍在蛋白羧基末端水解同工酶和丙糖磷酸异构酶。

关键词：蛋白质组肽质量指纹谱人肺癌细胞双向电泳基质辅助激光解吸/电离飞行时间质谱

Characterization of rtSHSp13 gene encoding a development protein involved in vesicular traffic in spermiogenesis

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Cell Research 2004年第3期 197-207页

作者： Samuel S. KOIDE center for biomedical Research Population Council 12.0 York Avenue New York NY 1002. USA

A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312.amino acids and was assigned the GenBank accession number AF2.7439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor （EGFR） and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.

关键词： rtSH3p13 spermiogenesis acrosome clathrin endocytosis.

Paclitaxel-induced stress granules increase LINE-1 mRNA stability to promote drug resistance in breast cancer cells

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The Journal of biomedical Research 2021年第6期35卷 411-424页

作者： Xiao Shi Xinxin Si Ershao Zhang Ruochen Zang Nan Yang He Cheng Zhihong Zhang beijing Pan Yujie Sun Key Laboratory of Human Functional Genomics of Jiangsu Province Nanjing Medical UniversityNanjingJiangsu 211166China Department of Cell Biology School of Basic Medical SciencesNanjing Medical UniversityNanjingJiangsu 211166China Collaborative Innovation center for Cancer Personalized Medicine Nanjing Medical UniversityNanjingJiangsu 211166China Department of Pathology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210036China.

Abnormal expression of long interspersed element-1(LINE-1)has been implicated in drug resistance,while our previous study showed that chemotherapy drug paclitaxel(PTX)increased LINE-1 level with unknown *** analysis suggested the regulation of LINE-1 mRNA by drug-induced stress granules(SGs).This study aimed to explore whether and how SGs are involved in drug-induced LINE-1 increase and thereby promotes drug resistance of triple negative breast cancer(TNBC)*** demonstrated that SGs increased LINE-1 expression by recruiting and stabilizing LINE-1 mRNA under drug stress,thereby adapting TNBC cells to chemotherapy ***,LINE-1 inhibitor efavirenz(EFV)could inhibit drug-induced SG to destabilize *** study provides the first evidence of the regulation of LINE-1 by SGs that could be an important survival mechanism for cancer cells exposed to chemotherapy *** findings provide a useful clue for developing new chemotherapeutic strategies against TNBCs.

关键词： LINE-1 stress granules RNA stability neoplasm drug resistance triple negative breast cancer

Bioceramics-based cartilage tissue engineering

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Bioceramics-based cartilage tissue engineering

The 6th International Conference on biomedical engineering and Rehabilitation engineering

作者： Wang Changyong Quo Ximin Bo Bin Lu Jianxi Zhao Qiang Duan Cuimi Tissue engineering Research center of Academy of Military Medical Sciences {2.100850 china E-mail: wcy2.00@***

＜正＞ Various biomaterials have been developed yet proved to be far from ideal for cartilagetissue engineering. In this study we introduced a new scaffold, bioceramics, to generatecartilage replacement. Beta-TCP porous ceramics seeded with sheep chondrocytes wereimplanted subcutaneously in a nude mice model and in sheep articular cartilage defects.

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A Feasibility Study of FES Restoration of Elbow Movementsfor C5/C6 Quadriplegic Patients

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Chinese Journal of biomedical engineering(English Edition) 1998年第3期7卷 119-122页

作者： N. Lan J.C. Wang P. Lin and Y. Li(Dept. of Electrical engineering, Tsinghua univer.ity{2. 100084, P. R. china)(Spinal Cord injury Unit, Charity Hospitalchina Rehabilitation Research center, {2. 100077, P. R. china)

This paper presents a feasibility study of restoration of elbow movements in C5/C6quadriplegic patientS by functional electrical stimulation (FES). A biomechanical model of thebiceps and triceps muscles and elbow joint is developed for computer simulation. A feedbackcontrol method is evaluated in the study for the static and dynamic responses. Results indicate thatit is possible to achieve extension control at the elbow joint with electrical stimulation of extensormuscle. These results may provide a plausible control method for controlling elbow extensionmovements in clinical application.

关键词： Functional Electrical Stimulation (FES) Rehabilitation engineering Motor Control