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Surface investigation on Upilex-S polymide film iradiated by vacuum ultraviolet radiation

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Rare Metals 2007年第S1期26卷 168-174页

作者： LIU Yuyan1),GONG Xianyun2.,WAN Zhimin3),and DU Xingwen4)1) Department of Applied Chemistry,Harbin Institute of Technology,Harbin 150001,China 2. Department of Life Science and Chemistry,Harbin university,Harbin 150001,China 3) Analysis and Measurement center,Harbin Institute of Technology,Harbin 150001,China 4) center for Composite Materials,Harbin Institute of Technology,Harbin 150001,China Harbin Inst Technol Dept Appl Chem Harbin 150001 Peoples R China Harbin Univ Dept Life Sci & Chem Harbin 150001 Peoples R China Harbin Inst Technol Ctr Anal & Measurement Harbin 150001 Peoples R China Harbin Inst Technol Ctr Composite Mat Harbin 150001 Peoples R China

Vacuum ultraviolet radiation of Upilex-S polyimide film 2. μm thick was performed using a gas jet type of vacuum ultraviolet simulator which gives unfixed wavelength from 5 nm to 2.0 *** redeposited on the films were observed and the nanoparticles formed on the surface consist mainly of carbon *** in the composition and the chemical characteristics of film surface after radiation were identified by X-ray photoelectron spectroscopy(XPS).Relative O content in the radiated area was found to be higher,while N content was lower than in the pristine *** indicates that Upilex-S polyimide film releases N atoms as volatile *** the C1s,O1s envelopes were fitted using Multipak Spectrum *** surface morphologies on iradiated area were examined by atomic Force Microscope(AFM).Pieces of radiant products protrude from the eradiated surface,leading to considerable roughness for the iradiated *** the surface transmittance of Upilex-S film after radiation reduces.

关键词： vacuum ultraviolet radiation Upilex-S polyimide film

Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2.Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of molecular Microbiology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.0032.China 2.Department of Medicinal Chemistry,School of Pharmacy,Fudan university,Shanghai 2.0032.China 3 State Key Laboratory of Genetic Engineering & Department of Microbiology,School of Life Science,Fudan university,Shanghai 2.0433,China 4 Laboratory of Disease and Health Genomics,Chinese National Human Genome center at Shanghai,Shanghai 2.12.3,China Laboratory of molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32.were *** experiments showed that the promoter of U32.glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2.located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32.pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32.pglnA in *** required GlnR,and its binding to the U32.pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32.glnR allowed usto detect the in vitro interaction between the U32.GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

molecular dynamics simulations of interaction between protein-tyrosine phosphatase 1B and a bidentate inhibitor

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Acta Pharmacologica Sinica 2006年第1期27卷 100-110页

作者： Gui-xia LIU~(2.3,4) Jin-zhi TAN~2.Chun-ying NIU~2.Jian-hua SHEN~(2.3) Xiao-min LUO~2.Xu SHEN~(2.3) Kai-xian CHEN~2.Hua-liang JIANG~(2.3,4)2.center for Drug Discovery and Design,State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.12.3,China 3 School of Pharmacy,East China university of Science and Technology Shanghai 2.02.7,China centerforDrugDiscoveryandDesign StateKeyLaboratoryofDrugResearchShanghaiInstituteofMateriaMedicaShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai201203China

Aim:To investigate the dynamic properties of protein-tyrosine phosphatase (PTP)1B and reveal the structural factors responsible for the high inhibitory potencyand selectivity of the inhibitor SNA for ***:We performed moleculardynamics (MD) simulations using a long time-scale for both PTP1B and PTP1Bcomplexed with the inhibitor SNA,the most potent and selective PTP1B inhibitorreported to *** trajectories were analyzed by using principal ***:Trajectory analyses showed that upon binding the ligand,theflexibility of the entire PTP1B molecule *** most notable change is themovement of the *** simulation results also indicated that electro-static interactions contribute more to PTP 1B-SNA complex conformation than thevan der Waals interactions,and that Lys41,Arg47,and Asp48 play important rolesin determining the conformation of the inhibitor SNA and in the potency andselectivity of the *** these,Arg47 contributed *** results werein agreement with previous experimental ***:The informationpresented here suggests that potent and selective PTP1B inhibitors can be de-signed by targeting the surface residues,for example the region containing Lys41,Arg47,and Asp48,instead of the second phosphate binding site (besides theactive phosphate binding site).

关键词： molecular dynamics simulation principal component analysis protein-tyrosine phosphatase 1B type 2.diabetes

On four closely related hypotrichous ciliates(Protozoa,Ciliophora,Spirotrichea):molecular characters,interspecific relationships and phylogeny defined with multigene sequence information

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Acta Oceanologica Sinica 2010年第5期29卷 90-96页

作者： GAO Feng YI Zhenzhen CHEN Zigui AL-RASHEID Khaled A S SONG Weibo Laboratory of Protozoology Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education College of Life Science South China Normal University Guangzhou 510631 China Laboratory of Protozoology KLM Ocean University of China Qingdao 266003 China Zoology Department King Saud University Riyadh 11451 Saudi Arabia

In order to clarify the phylogeny and relationships of the most confused hypotrichous ciliates,Holosticha-complex,four closely related holostichids（five populations）,Holosticha bradburyae,***,Anteholosticha sp.,and ***,were compared and analyzed using ITS2.secondary structures,ITS1-5.8S-ITS2.region and SSrRNA gene *** ITS1-5.8S-ITS2.region sequences of these four species were first sequenced,and they shared sequence identities ranging from 68.0% to 90.1%,while two populations of Anteholosticha *** in three nucleotides（sequence identity 99.8%）.There were several minor differences among ITS2.secondary structures of these species,while two populations of Anteholosticha *** the identical secondary *** trees inferred from the ITS1-5.8S-ITS2.region sequences of stichotrichs using multiple algorithms（Neighbor-Joining,Maximum Parsimony and Bayesian） revealed similar *** results show that：（1） Holosticha bradburyae and *** firmly clustered together with strong bootstrap supports,forming a sister clade with Anteholosticha sp.,（2. Anteholosticha appeared to be a paraphyletic assemblage,in which the morphotype *** was more closely related to Diaxonella trimarginata than to its congener Anteholosticha *** analyses based on the SSrRNA gene and the combined sequences of SSrRNA gene and ITS1-5.8S-ITS2.region revealed the similar relationships between Holosticha and Anteholosticha,nevertheless their positions within the subclass Stichotrichia differed from each other inferred from different genes.

关键词： phylogeny gene sequencing marine ciliates SSrRNA ITS1-5.8S-ITS2

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第五届亚洲儿科研究大会

作者： Jia Hou~1 Yonghao Gui~1 Yuexiang Wang~2.Houyan Song~2.1.Cardiovascular center Children’s Hospital of Fudan university 2.National Key Laboratory of {2. Medicine Fudan university 2.1102 Cardiovascular center Children''s Hospital of Fudan University National Key Laboratory of Molecular MedicineFudan University 201102

＜正＞Objectives To set up a model of retinal dehydrogenase type 2.raldh2. gone knock-down zebrafish embryos, and to study the effect of insufficient retinoid acid（RA） signaling on early embryonic cardiac developm...

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CUO基态分子热力学稳定性研究

物理学报 2003年第12期52卷 2965-2968页

作者：薛卫东朱正和四川师范大学化学系成都610066 四川大学原子分子物理研究所成都610065

根据热力学原理、Debye固体理论和量子力学从头计算的分子结构 ,运用统计热力学方法计算得到物质的热力学函数值 .由算得的CUO(g)分子在不同温度下的标准生成自由能变 ,表明了在高温下可能有CUO(g)分子的稳定存在 .

关键词： CUO分子热力学函数稳定性 Debye固体理论量子力学计算统计物理学电子运动

Immunization of mice with cells from juvenile worms of Schistosoma japonicum provides immunoprotection against schistosomiasis

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Science China(Life Sciences) 2007年第6期50卷 822-830页

作者： ZENG TieBing1,2. CAI LiTing1, ZENG QingRen1, YANG ShengHui1, YU Rong1, LI YanQin1, FANG HuiLong1, LI BenWen3 & ZHANG ShunKe1 1 Cell & molecular Biological Experiment center and Department of Parasitology, Xiangya School of Medicine, Central South Uni- versity, Changsha 410013, China 2.Institute of Pathogenic Biology, Medical College, Nanhua {2., Hengyang 42.001, China 3 Infectious Diseases Division, Washington {2. School of Medicine, Washington 63110, USA Cell & molecular Biological Experiment center Department of Parasitology Xiangya School of Medicine Central South University Changsha 410013 China Institute of Pathogenic Biology Medical College Nanhua University Hengyang 420001 China Infectious Diseases Division Washington University School of Medicine Washington 63110 USA

To validate the protective efficacy against schistosomiasis by immunization with cells from juvenile Schistosoma japonicum in a murine model; to analyze possible factors related to protection, in this study, two independent repeated vaccination trials were performed. After three subcutaneous vaccinations, in trial one, in the absence of adjuvant, primary juvenile worm cells (pJCs) from S. japonicum induced remarkable average reductions in worm burden (54.3%), liver eggs per gram (LEPG) load (59.8%) as well as egg granulomas size (66.5%) compared to PBS control group (P2.type of immune response while cACs elicited a Th2.type response. Our data indicated that immunization with both primary; cultured cells from S. japonicum juvenile worms provided high immunoprotection, for which the physical character of immunogens, stage-specific parasite; the type of immune response induced might be responsible, suggesting that vaccination with whole cells from S. japonicum larvae is a promising approach to produce protective immunity against schistosomiasis.

关键词： Schistosoma japonicum, juvenile worm cell, cultured cell, immunoprotection, immune response

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Editorial

Science China(Life Sciences) 2010年第2期53卷 157-158页

作者： GenHong Cheng (1) YongJun Liu (2. Hong Tang (3) 1. Department of Microbiology Immunology & Molecular Genetics University of California Los Angeles 609 Charles E. Young Drive East Los Angeles California 90095 USA2. Department of Immunology and Center for Cancer Immunology Research University of Texas M.D. Anderson Cancer Center Houston Texas 77030 USA3. Key Laboratory of Infection and Immunity Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

SM (spatial multiplexing) can effectively increase the information rate in multiple input multiple output system. BLAST is the typical representation of SM, especially VBLAST, which has some simple de- tection algorithms such as ML, ZF-DFE and ML-DFE, etc. However, the existing algorithms cannot approach ML performance. This paper discusses the effect for detection performance by the correlation of channel ma- trix, proposes a new algorithm—HPML detection algorithm, which can approach ML performance with low complexity. In the new algorithm, we travel the first d layers, and use the DFE procedure for the remaining layers, then perform ML detection for all obtained signals. Simulation results show that HPML can approach ML performance when the traveling numbers are not less than half of the number of transmitting antennas, and the algorithm complexity is smaller than ML.

关键词： BLAST correlation of channel matrix ML detecting algorithm HPML detecting algorithm

Structures and properties of novel 5,15-di[4-(5-acetylsulfanylpentyloxy)phenyl] porphyrin derivatives:Density functional theory calculations

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Science China Chemistry 2010年第10期53卷 2183-2192页

作者： ZHU YiFei1,2. QI DongDong1, ZHANG LiJuan1, WAN Liang1, ZHANG YueXing1 & JIANG JianZhuang1 1Department of Chemistry, university of Science and Technology Beijing, Beijing 100083, China 2.epartment of Chemistry, Hebei North university, Zhangjiakou 075000, China 1. Department of Chemistry University of Science and Technology Beijing Beijing 100083 China2. Department of Chemistry Hebei North University Zhangjiakou 075000 China

Density functional theory (DFT) calculations have been carried out in order to compare the molecular structures, atomic charges, molecular orbitals, electronic absorption spectra, and infrared (IR) spectra of the metal-free 5,15 -di[4-(5-acetylsul-fanylpentyloxy)phenyl]porphyrin H2.DPP(OC5H10SCOCH3)2. (1) (DPP = 5,15-diphenylporphyrin) and its zinc complex Zn[DPP(OC5H10SCOCH3)2. (2., which exhibit novel structures with two 5-acetylsulfanylpentyloxy side chains at the para-positions of opposite meso-attached phenyl groups. The calculated molecular structure and electronic absorption and IR spectra of 1 and 2.are consistent with the experimental results. The influences of meso-substitution, polar solvents, and central metal substitution on the structure and properties of porphyrin derivatives have been investigated by comparing the corresponding data for 1 and 2.with the help of data for the unsubstituted porphyrin derivatives, namely the metal-free porphyrin H2.or (3) and the porphyrinato-zinc compound (4). The identities of the main transitions in the electronic absorption spectra of 1 and 2.are assigned and the vibrational modes in their IR spectra are identified with the assistance of animated pictures produced based on normal coordinates. The theoretical work presented here will be helpful in increasing our understanding of the structure and spectroscopic properties, as well as substituent and solvent effects, for these novel porphyrin compounds.

关键词： porphyrin substituent effect electronic absorption spectra IR spectra density functional theory (DFT)