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水产学报 2009年第3期33卷 410-416页

作者：罗鹏胡超群中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室广东省应用海洋生物学重点实验室

溶藻弧菌的胞外产物(ECP)在其致病过程中发挥重要作用,已经观察到溶藻弧菌ECP所致的溶血现象,关于溶藻弧菌溶血素的种类和其对溶藻弧菌的致病性贡献的报道非常稀少。利用已经报道的多种弧菌溶血素基因序列设计通用引物,检测溶血素基因... 详细信息

溶藻弧菌的胞外产物(ECP)在其致病过程中发挥重要作用,已经观察到溶藻弧菌ECP所致的溶血现象,关于溶藻弧菌溶血素的种类和其对溶藻弧菌的致病性贡献的报道非常稀少。利用已经报道的多种弧菌溶血素基因序列设计通用引物,检测溶血素基因在溶藻弧菌中的分布,发现96个菌株中有74株扩增出溶血素基因(vah)片段。对致病株ZJ0451的vah片段测序。根据已测得的片段序列设计引物,通过反向PCR扩增及后续克隆测序获得全长vah基因及部分侧翼序列。经比对证实溶藻弧菌vah与多种弧菌的TLH类溶血素高度相似,且其肽链N端有信号肽。利用pET32.载体构建了两种包含不同长度融合标签的vah表达载体,并均在大肠杆菌BL2.(DE3)中获得可溶性表达。在2.℃的诱导温度下,9 h时vah表达量达到相对最大。在原核表达系统中,vah蛋白信号肽可以被切除。

关键词：溶藻弧菌溶血素反向PCR 表达

INVESTIGATION ON SIGNAL REGULATION OF EUPATORIUM ADENOPHORUM ON ITS ALLELOPATHIC EFFECTS

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INVESTIGATION ON SIGNAL REGULATION OF EUPATORIUM ADENOPHORUM...

Satellite Tracking of Post-nesting Migration of Green Turtles

International Congress on Biological Invasions

作者： Jian YAN~1,Hai-Hong BI~1,Ya-Nan JIN~2.Fang-Hao WAN~2.and Jian-Wen TAN~1 1 cas key {2. of Plant Resources Conservation and Sustainable Utilization,South china Botanic Garden,chinese academy of sciences,guangzhou 510650,china. 2.State key {2. for Biology of Plant Disease and Insect Pests,{12. of Plant Protection,chinese academy of Agricultural sciences,Beijing 100094,china

Eupatorium adenophorum Spreng.,native to Mexico,is well-known as an invasive plant in many tropical and subtropical countries of the world as well as in *** exotic plant is extremely ecologically destructive to local biodiversity at its new locations and its rapid spreading has caused great economic losses in those seriously invaded *** far,though diverse studies have indicated that allelopathy would be one of the key factors for the invasive success of this noxious plant,the answers for the two questions abour what and how allelochemicals are involved in its allelopathic strategy are still largely ***,we started a new research to investigate the potential significance of defence-related signal regulation of E. adenophorum on its allelopathic *** this presentation,progress in our recent study on investigating the potential significance of defence-related signal regulation of *** on its allelopathic effects will be *** details of the progress will include the designation and preparation of defence-related signals and their functional mimics,the detection of endogenous signals in fresh tissues by GC-NCI-MS based analytic methods,the characterization of potential allelochemicals in root exudates,the analysis of signal-induced secondary metabolites and their secretion or exudation from living ***,some related interesting ongoing works including the identification of new chemical constituents from fresh living tissue extracts of *** will also be briefly presented and discussed.

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Bulletin of the chinese academy of sciences 2002年第2期16卷 102-105页

作者： Wang Wenzhi, Wang Dongxiao , Wang Huajie & Song Xiaojun(key laboratory of Tropical Marine Environment Dynamics, South{2. sea institute of Oceanology, cas, 510301 guangzhou,{2.)4 (South{2. institute for Endangered Species) key laboratory of Tropical Marine Environment Dynamics South China Sea Institute of OceanologyCAS510301 GuangzhouChina South china institute for Endangered Species

During the period August 17-2., 2.01, in collaboration with the Provincial Bureau of Oceanography & Fisheries of Guangdong and the South china institute for Endangered Species, the South china sea institute of Oceanology, a cas affiliate in the city of guangzhou, conducted a sea turtle satellite tracking project at Haigui Bay (Fig. 1) in the vicinity of Gangkou Town, Huidong County, Guangdong Province.

关键词： Satellite Tracking of Post-nesting Migration of Green Turtles Bay

对近海四分量地震技术研究与应用的认识和思考

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天然气地球科学 2004年第1期15卷 37-41页

作者：张树林夏斌李绪宣中国科学院广州地球化学研究所与南海海洋研究所边缘海地质重点实验室广东广州510640

从我国近海的地质和纵波地震勘探现状来看,四分量地震技术能够解决一些勘探难题。该项技术引入我国还只有4年时间,在1998-2.00年之间开展了处理和解释方法研究,并在莺歌海盆地进行了试验,之后,国外石油公司分别在合作区块蓬莱19-3油田... 详细信息

从我国近海的地质和纵波地震勘探现状来看,四分量地震技术能够解决一些勘探难题。该项技术引入我国还只有4年时间,在1998-2.00年之间开展了处理和解释方法研究,并在莺歌海盆地进行了试验,之后,国外石油公司分别在合作区块蓬莱19-3油田采集了三维四分量地震资料、在崖城13-1气田采集了2.测线的二维四分量地震资料。从我国的试验情况来看,四分量地震勘探在解决气烟囱成像、小断层及低幅度构造识别、岩性及浅层气藏预测方面的效果是很好的,在各向异性研究方面也能起到较好的效果。认为我国在四分量地震技术研究方面还需要加强,还需要继续投入大量资金;虽然在四分量地震技术方面还有一些问题有待解决,但它的应用前途是光明的,因为单一的纵波勘探必将走向多分量勘探。

关键词：四分量地震新事物气烟囱气藏预测各向异性

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桂林乌桕中的香豆素和鞣花酸类化合物

天然产物研究与开发 2004年第4期16卷 297-299页

作者：漆淑华吴大刚罗晓东中国科学院南海海洋研究所广东省海洋药物重点实验室广州510301 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室昆明650204

从桂林乌桕枝条的乙醇提取物中分离鉴定了 8个已知化合物 ,经波谱分析确定其结构分别为 :6 ,7,8 三甲氧基香豆素 (1)、5 ,6 ,7,8 四甲氧基香豆素 (2.)、8 羟基 5 ,6 ,7 三甲氧基香豆素 (3)、东莨菪内酯 (4 )、3,3’ 二甲氧基鞣花酸 (5 ... 详细信息

从桂林乌桕枝条的乙醇提取物中分离鉴定了 8个已知化合物 ,经波谱分析确定其结构分别为 :6 ,7,8 三甲氧基香豆素 (1)、5 ,6 ,7,8 四甲氧基香豆素 (2.)、8 羟基 5 ,6 ,7 三甲氧基香豆素 (3)、东莨菪内酯 (4 )、3,3’ 二甲氧基鞣花酸 (5 )、4 ,4’ 二甲氧基鞣花酸 (6 )、3,3’ 二甲氧基鞣花酸 4 O β D 木吡喃苷 (7)、3,3’ 二甲氧基鞣花酸 4 O β D 葡萄吡喃苷 (8) ;其中化合物 2.,3,6 ,7。

关键词：桂林乌桕香豆素鞣花酸类化合物大戟科波谱分析

Hyperspectral retrieval model of phycocyanin in case II waters

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Science Bulletin 2006年第S2期 149-153页

作者： YANG Dingtian1 & PAN Delu2.1.LED,South china sea {2. of Oceanography,guangzhou 510301,china 2.State key laboratory of Satellite Ocean Environment Dynamics,Second {2. of Oceanography,SOA,Hangzhou 310012.china

Phycocyanin, a functional protein found in blue-green algae, with characteristic absorption peak around 62. nm, can be used to detect the quantity of blue-green algae in waters. Spectral characteristics of phycocyanin were studied by measuring hyperspectral water leaving radiance and absorption curve, and the results showed that the absorption peak of phycocyanin around 62. nm was evident in the curve of water leaving radiance. Bio-optical model of phycocyanin was set up with analytical methods and calibrated with error analysis. Linear relationship between phycocyanin concentration measured and that retrieved with calibrated bio-optical model (R = 0.755) was better than that retrieved with the band ratio of 709 nm/62. nm (R = 0.72.), which proved that the calibration was neces-sary for improving the accuracy of phycocyanin concentration.

关键词： phycocyanin case II waters hyperspectral remote sens-ing model calibrated algorithm.

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鱼类白细胞介素1β基因的研究概况

水产学报 2005年第1期29卷 115-119页

作者：邱丽华郭奕惠张汉华中国科学院海洋研究所实验海洋生物学开放实验室中国水产科学研究院南海水产研究所中国水产科学研究院南海水产研究所山东青岛　2.6071中国水产科学研究院南海水产研究所广东广州　510300

Interleukin-1β(IL-1β) is one of the most pleiotropic cytokines and a central regulator of the immune and inflammatory responses. Interleukin-1β was initially discovered within mice and humans and over the last 10 years has been characterized within a wide variety of animals. The IL-1β plays a key role in the inflammatory process, enhancing cell-mediated immunity by inducing the growth and proliferation of lymphocytes, connective tissue cells, and by stimulating immune and inflammatory response effector cells. As an immunoregulatory cytokine, IL-1β has the potential to enhance the immune response induced by a vaccine and/or to modulate the immune response leading to different effector mechanisms. It is produced by many cell types, including monocytes, macrophages, T and B lymphocytes, fibroblasts, and endothelial and epithelial cells. Expression is induced by a diverse range of stimuli, including mitogens, cytokines, and microbial products. There have been considerable evidences provided by biological cross reaction that fish produce IL-1β during immune responses, and the bioactivity of IL-1 in fish has been known for over a decade. But only since 1999, IL-1β gene has been cloned from the rainbow trout. And from then on, IL-1β gene has been cloned and expressed in many fish confirming that fish produces IL-1β gene during immune responses. In mammals it is produced as an inactive precursor that is processed by interleukin converting enzyme (ICE) to give a biologically active `mature’ peptide. There is no signal peptide in IL-1β and its mechanism is unknown. This is the special part of the gene structure of the IL-1β. And through the program analyzing, we found this special structure in fish IL-1β gene. This paper reviews the functions and structure of gene IL-1β and the studies of the gene IL-1β in fish recently.

关键词：细胞因子白细胞介素1β 鱼类

Heat shock response and mammal adaptation to high elevation(hypoxia)

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Science china(Life sciences) 2006年第5期49卷 500-512页

作者： WANG Xiaolin1,2.3,XU Cunshuan4,WANG Xiujie5,WANG Dongjie6,WANG Qingshang7 & ZHANG Baochen11.Northwest Plateau Biological Research institute,{2. academy of sciences,Xining 810001,china 2.Graduate School of the chinese academy of sciences,Beijing 100039,china 3.Affiliated Hospital of Medical College of Qinghai University,Xining 810001,china 4.key laboratory of Ministry of Science and Technology of china in Henan Normal University,Xinxiang 453000,china 5.key laboratory of china Ministry of Education in Oncology Research institute of Western Hospital of Sichuan University,Chengdu 610041,china 6.guangzhou Meizheng Pharmaceutical Industry & key laboratory of Ministry of Health of china,guangzhou 510075,china 7.Rubber Research institute,china Tropical Agricultural academy of sciences,Haikou 571737,china Northwest Plateau Biological Research institute Chinese Academy of Sciences Xining 810001 China Graduate School of the chinese academy of sciences Beijing 100039 China Affiliated Hospital of Medical College of Qinghai University Xining 810001 China key laboratory of Ministry of Science and Technology of{2. in Henan Normal University Xinxiang 453000 China key laboratory of{2. Ministry of Education in Oncology Research institute of Western Hospital of Sichuan University Chengdu 610041 China guangzhou Meizheng Pharmaceutical Industry & key {2. of Ministry of Health of china Guangzhou 510075 China Rubber Research institute China Tropical Agricultural Academy of Sciences Haikou 571737 China Northwest Plateau Biological Research institute Chinese Academy of Sciences Xining 810001 China

The mammal's high elevation(hypoxia) adaptation was studied by using the immu-nological and the molecular biological methods to understand the significance of Hsp(hypoxia) ad-aptation in the organic high elevation,through the mammal heat shock response.(1) From high ele-vation to low elevation(natural hypoxia) :Western blot and conventional RT-PCR and real-time fluo-rescence quota PCR were *** difference of heat shock protein of 70(Hsp70) and natural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals(yak) .(2. From low elevation to high elevation(hypoxia induction) :The mammals(domestic rabbits) from the low elevation were sent directly to the areas with different high elevations like 2.00,3300 and 5000 m above sea level to be raised for a period of 3 weeks be-fore being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 was deter-mined with *** result indicated that all of the mammals at different elevations possessed their heat shock response ***70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high elevation(hypoxia) .The speedy synthesis of Hsp70 in the process of heat shock response is suitable to maintain the cells' normal physiological functions under *** Hsp70 has its threshold *** altitude of 5000 m above sea level is the best condition for the heat shock response,and it starts to reduce when the altitude is over 6000 m above sea *** Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio.

关键词： heat shock reaction, heat shock protein 70 (Hsp70), Western blot, RT-PCR, fluorescence quota PCR, high elevation hypoxia, mammal, stress.

UVR-energized CO2.fixation by marine phytoplankton:Cell-size dependent

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UVR-energized CO2 fixation by marine phytoplankton:Cell-size...