检索结果-南通市图书馆

Chinese Science Bulletin 1999年第8期44卷 715-719页

作者： WU Zhijian, WU Xiaobing and HOU YundeState Key Laboratory for Molecular Virology and Genetic Engineering , institute of Virology , Chinese Academy of Preventive medicine , beijing 100052.{2. State Key Laboratory for Molecular Virology and Genetic Engineering Institute of Virology Chinese Academy of Preventive Medicine Beijing China

The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos2., cos6, cos14 and cos56, which represents the HSV-1 genome was used for generation of this rHSV. Rep and cap genes of AAV-2.were inserted into Xba Ⅰ site of UL2.gene on cos6, generating cos6-rcΔUL2. After being digested with Pac Ⅰ , cos6-rcΔUL2.and the other 4 cosmids were cotrans-fected into BHK-2. cells. The recombinant virus HSV1-rc/ΔUL2.carrying rep and cap genes was generated due to the homologous recombination of the 5 cosmids. The results showed that the existence of rep and cap genes on this rHSV was stable from passage to passage and the rHSV could support the packaging of rAAV either in cells transiently transfected with AAV vector or in stable cell line harboring AAV vector. Further modification of this rHSV and optimization of conditions involved in rAAV preparation may lead to a large-scale production of rAAV in the near future.

关键词： recombinant AAV packaging function recombinant HSV.

Risk analysis system of geo-hazard based on GiS technique

在线全文

学校读者我要写书评

暂无评论

Journal of Geographical Sciences 2002年第3期12卷 371-376页

作者： ZHU Liangfeng, ZHANG Guirong, YiN Kunlong, ZHANG Liang (1. Faculty of Engineering, china University of Geosciences, Wuhan 430074, china 2. china Research institute of Land Resources and Economy, beijing 101149, china) Faculty of Engineering China University of Geosciences(Wuhan) Wuhan 430074 china Research institute of Land Resources and Economy 北京 101149

The socio-economic attribute of geo-hazard made us distinguish it from the traditional engineering geology study. it will get more social benefit from the analysis of the geo-hazard in the socio-economic attribute. The hazard and the vulnerability of the element controls the risk level of the regional geo-hazard. The risk analysis supported by GiS in geo-hazard study is one of the most important directions. Based on the author’s studies in recent years, a risk analysis system of regional geo-hazard (RiskAnly) has been developed on the basis of software MAPGiS. The paper introduces the train of system design, the structure and the workflow of RiskAnly. As a case study, the paper also deals with the risk zonation of the regional landslide hazard of china.

关键词： geo-hazard GiS risk analysis landslide

Hawking radiation and thermodynamics of a Vaidya-Bonner black hole

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2010年第1期10卷 33-38页

作者： Zhen-Feng Niu Wen-Biao Liu Department of Physics Hebei North University Zhangjiakou 075000 China Department of Physics Institute of Theoretical Physics Beijing Normal University Beijing 100875 China

Using Parikh＇s tunneling method, the Hawking radiation on the apparent horizon of a Vaidya-Bonner black hole is calculated. When the back-reaction of particles is neglected, the thermal spectrum can be precisely obtained. Then, the black hole thermodynamics can be calculated successfully on the apparent horizon. When a relativistic perturbation is applied to the apparent horizon, a similar calculation can also lead to a purely thermal spectrum. The first law of thermodynamics can also be derived successfully at the new supersurface near the apparent horizon. When the event horizon is thought of as a deviation from the apparent horizon, the expressions of the characteristic position and temperature are consistent with the previous viewpoint which asserts that the thermodynamics should be based on the event horizon. it is concluded that the thermodynamics should be constructed exactly on the apparent horizon while the event horizon thermodynamics is just one of the perturbations near the apparent horizon.

关键词： black hole physics -- relativity -- kinematics and dynamics

Ginsenoside Rg1 inhibits proliferation of vascular smooth muscle cells stimulated by tumor necrosis factor-α

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2006年第8期27卷 1000-1006页

作者： Zeng-chun MA Yue GAO~2.Yu-guang WANG Hong-ling TAN Cheng-rong XiAO Sheng-qi WANG institute of radiation medicine Sciences,Academy of Military Medical Sciences,{2. 100850,china institute of radiation medicine Sciences Academy of Military Medical Sciences Beijing 100850 China

Aim:To investigate the proliferation of vascular smooth muscle cells(VSMC)affected by ginsenoside Rg1 and further explore the molecular mechanism ofginsenoside Rg1 using ***:The proliferation of VSMC wasmeasured by MTS assay kit and flow *** alterations were ana-lyzed using two-dimensional electrophoresis and peptide mass *** proteins found in proteomics were confirmed by ***:The proliferation of VSMC was enhanced significantly after tumor necrosis fac-tor-α(TNF-α)treatment,and ginsenoside Rg1 treatment inhibited proliferation ina dose-dependent *** analysis showed 2. protein spots werechanged,including 17 spots that were increased and 7 spots that were *** Rg1 could restore the expression levels of these proteins,at leastpartly,to basic levels of untreated *** expression of G-protein coupledreceptor kinase,protein kinase C(PKC)-ζ,N-ras protein were decreased,whilecycle related protein p2. was increased by ginsenoside Rgl in TNF-α ***:PKC-ζ and p2. pathway might be the mechanism for inhibi-tory effects of ginsenoside Rg1 on proliferation of VSMC.

关键词： cell cycle ginsenoside Rg1 proteomics tumor necrosis factor-α vascular smooth muscle cells

太阳辐射下不同地表覆盖物的热反应及对城市热环境的影响

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

太阳能学报 2002年第6期23卷 717-720页

作者：邢永杰沈天行刘芳天津大学建筑学院天津100072 北京市太阳能研究所北京100083

该文利用红外热像仪等设备研究了建筑物表面、绿地、柏油路面、水泥砖地面等城市地表覆盖物对太阳辐射的热反应 ,指出在同一天中 ,柏油路面温度最高 ,水泥砖和建筑物表面次之 ,绿地温度最低。通过分析各种覆盖物在城市中所占比例 ,得出... 详细信息

该文利用红外热像仪等设备研究了建筑物表面、绿地、柏油路面、水泥砖地面等城市地表覆盖物对太阳辐射的热反应 ,指出在同一天中 ,柏油路面温度最高 ,水泥砖和建筑物表面次之 ,绿地温度最低。通过分析各种覆盖物在城市中所占比例 ,得出建筑物对城市热环境影响较大的结论 ,并提出了改善城市热环境的几种方法。

关键词：太阳辐射城市地表覆盖物热反应红外热像仪热环境热岛效应

Dynamic information theory and information description of dynamic systems

在线全文

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 607-627页

作者： XiNG XiuSan Department of Physics, beijing institute of Technology, beijing 100081, {2. 1. Department of Physics Beijing Institute of Technology Beijing 100081 China

in this paper, we develop dynamic statistical information theory established by the author. Starting from the ideas that the state variable evolution equations of stochastic dynamic systems, classical and quantum nonequilibrium statistical physical systems and special electromagnetic field systems can be regarded as their information symbol evolution equations and the definitions of dynamic information and dynamic entropy, we derive the evolution equations of dynamic information and dynamic entropy that describe the evolution laws of dynamic information. These four kinds of evolution equations are of the same mathematical type. They show in unison when information transmits in coordinate space outside the systems that the time rate of change of dynamic information densities originates from their drift, diffusion and dissipation in state variable space inside the systems and coordinate space in the transmission processes, and that the time rate of change of dynamic entropy densities is caused by their drift, diffusion and production in state variable space inside the systems and coordinate space in the transmission processes. When space noise can be neglected, an information wave will appear. if we only consider the information change inside the systems, dynamic information evolution equations reduce to information equations corresponding to the dynamic equations which describe evolution laws of the above dynamic systems. This reveals that the evolution laws of respective dynamic systems can be described by information equations in a unified fashion. Hence, the evolution processes of these dynamic systems can be abstracted as the evolution processes of information. Furthermore, we present the formulas for information flow, information dissipation rate, and entropy production rate. We prove that the information production probably emerges in a dynamic system with internal attractive interaction between the elements, and derive a formula for this information production

关键词： dynamic information evolution equation informationization of dynamic equation information wave information flow and force information dissipation and production degradation and evolution

Geometry of Broad Line Regions of Active Galactic Nuclei

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Astronomy and Astrophysics 2008年第1期8卷 50-62页

作者： Xiao-Rong Lü1 Key Laboratory for Particle Astrophysics, institute of High Energy Physics, Chinese Academy of Sciences, beijing 100049 2.Graduate School of Chinese Academy of Sciences, beijing 100049 Key Laboratory for Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 and Graduate School of Chinese Academy of Sciences Beijing 100049

it has long remained an open question as to the geometry of the broad line region （BLR） in active galactic nuclei （AGNs）. The reverberation mapping technique which measures the response of the broad emission lines to the ionizing continuum, when combined with multiwavelength continuum fitted by sophisticated accretion disks, provides a way of probing the BLR geometry. We analyze a sample of 35 AGNs, which have been monitored by the reverberation mapping campaign. in view of energy budget, the reverberation-based BH masses are found to be in agreement with those obtained by accretion disk models in two thirds of the present sample while the reverberation mapping methods underestimate the BH masses in about one third of objects, as also suggested by CoUin et al. in a recent work. We point out that there are obviously two kinds of BLR geometry, which are strongly dependent on the Eddington ratio, and separated by the value LBol/LEdd - 0.1. These results prefer a scenario of the disk and wind configuration of the BLR and identify the Eddington ratio as the physical driver regulating the wind in the BLR.

关键词： accretion, accretion disks - galaxies active - galaxies Seyfert - quasars general

On the fundamental equation of nonequilibrium statistical physics—Nonequilibrium entropy evolution equation and the formula for entropy production rate

在线全文

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2010年第12期53卷 2194-2215页

作者： XiNG XiuSan Department of Physics, beijing institute of Technology, beijing 100081, {2. 1. Department of Physics Beijing Institute of Technology Beijing 100081 China

in this paper the author presents an overview on his own research works. More than ten years ago, we proposed a new fundamental equation of nonequilibrium statistical physics in place of the present Liouville equation. That is the stochastic velocity type’s Langevin equation in 6N dimensional phase space or its equivalent Liouville diffusion equation. This equation is time-reversed asymmetrical. it shows that the form of motion of particles in statistical thermodynamic systems has the drift-diffusion duality, and the law of motion of statistical thermodynamics is expressed by a superposition of both the law of dynamics and the stochastic velocity and possesses both determinism and probability. Hence it is different from the law of motion of particles in dynamical systems. The stochastic diffusion motion of the particles is the microscopic origin of macroscopic irreversibility. Starting from this fundamental equation the BBGKY diffusion equation hierarchy, the Boltzmann collision diffusion equation, the hydrodynamic equations such as the mass drift-diffusion equation, the Navier-Stokes equation and the thermal conductivity equation have been derived and presented here. What is more important, we first constructed a nonlinear evolution equation of nonequilibrium entropy density in 6N, 6 and 3 dimensional phase space, predicted the existence of entropy diffusion. This entropy evolution equation plays a leading role in nonequilibrium entropy theory, it reveals that the time rate of change of nonequilibrium entropy density originates together from its drift, diffusion and production in space. From this evolution equation, we presented a formula for entropy production rate (i.e. the law of entropy increase) in 6N and 6 dimensional phase space, proved that internal attractive force in nonequilibrium system can result in entropy decrease while internal repulsive force leads to another entropy increase, and derived a common expression for this entropy decrease rate or anothe

关键词： stochastic velocity type’s Langevin equation in 6N dimensional phase space drift-diffusion duality nonequilibrium entropy evolution equation entropy diffusion formula for entropy production rate entropy change from internal interaction a

肿瘤相关基因表达检测寡核苷酸芯片的制备及其初步应用

在线全文

学校读者我要写书评

暂无评论

生物化学与生物物理进展 2003年第6期30卷 900-905页

作者：黄坚杜清友丁雨王升启军事医学科学院放射医学研究所全军生物芯片重点实验室北京100850 深圳益生堂生物企业有限公司深圳518026

为研制肿瘤相关寡核苷酸芯片 ,并实现其在抗肿瘤反义核酸“癌泰得”作用机理研究方面的初步应用 ,制备了包含近 450种肿瘤相关基因特异寡核苷酸探针的寡核苷酸芯片 ,建立了相应的质控标准 .“癌泰得”用脂质体转染HepG2.瘤细胞 ,提取... 详细信息

为研制肿瘤相关寡核苷酸芯片 ,并实现其在抗肿瘤反义核酸“癌泰得”作用机理研究方面的初步应用 ,制备了包含近 450种肿瘤相关基因特异寡核苷酸探针的寡核苷酸芯片 ,建立了相应的质控标准 .“癌泰得”用脂质体转染HepG2.瘤细胞 ,提取细胞总RNA反转录并荧光标记cDNA ,用制备的寡核苷酸芯片检测肝癌细胞HepG2.肿瘤相关基因表达水平 ,用软件分析获得其差异基因表达谱 .0 4μmol/L的反义核酸“癌泰得”作用于HepG2.胞 15h后 ,MDNCF、DHS等基因mRNA表达下调 ,MUC2.MPP11、LAT、HRiF B、JNK3A1等mRNA基因表达上调 ,初步检测到了“癌泰得”的抗肿瘤作用可能的相关基因 ,为进一步的分子作用机理的探讨奠定基础 .结果表明 ,制备的肿瘤相关芯片敏感度高、特异性高、重复性均较好 ,可用于检测肿瘤相关基因的表达谱。

关键词：肿瘤相关基因寡核苷酸芯片癌泰得表达谱

A one-dimensional heat transfer model of the Antarctic ice Sheet and modeling of snow temperatures at Dome A, the summit of Antarctic Plateau

在线全文

学校读者我要写书评

暂无评论

Science china Earth Sciences 2010年第5期53卷 763-772页

作者： CHEN BaiLian1,3, ZHANG RenHe2. SUN ShuFen1, BiAN LinGen2. XiAO CunDe4,2.& ZHANG TingJun5 1 institute of Atmospheric Physics, Chinese Academy of Sciences, beijing 10002., china 2.Chinese Academy of Meteorological Sciences, beijing 100081, china 3 Graduate University of Chinese Academy of Sciences, beijing 100049, china 4 Cold and Arid Regions Environmental and Engineering Research institute, Chinese Academy of Sciences, Lanzhou 730000, china 5 The National Snow and ice Data Center, University of Colorado, Boulder, CO 80309-0449, USA 1. institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China3. Graduate University of Chinese Academy of Sciences Beijing 100049 China2. Chinese Academy of Meteorological Sciences Beijing 100081 China4. Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 China5. The National Snow and Ice Data Center University of Colorado Boulder CO 80309-0449 USA

A vertical one-dimensional numerical model for heat transferring within the near-surface snow layer of the Antarctic ice Sheet was developed based on simplified parameterizations of associated physical processes for the atmosphere, radiation, and snow/ice systems. Using the meteorological data of an automatic weather station (AWS) at Dome A (80°2.′S, 70°2.′E), we applied the model to simulate the seasonal temperature variation within a depth of 2. m. Comparison of modeled results with observed snow temperatures at 4 measurement depths (0.1, 1, 3, 10 m) shows good agreement and consistent seasonal variations. The model results reveal the vertical temperature structure within the near-surface snow layer and its seasonal variance with more details than those by limited measurements. Analyses on the model outputs of the surface energy fluxes show that: 1) the surface energy balance at Dome A is characterized by the compensation between negative net radiation and the positive sensible fluxes, and 2. the sensible heat is on average transported from the atmosphere to the snow, and has an evident increase in spring. The results are considered well representative for the highest interior Antarctic Plateau.

关键词： Antarctic ice Sheet near-surface layer energy transfer numerical model