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On the“plasticity”of adult stem cells and its application in regenerative medicine

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Chinese Science Bulletin 2003年第13期48卷 1392-1396页

作者： WANG Yunfang & PEI Xuetao Department of Stem Cell Biology, beijing Institute of transfusion {2., beijing 1.0850, china Correspondence should be addressed to Pei Xuetao (e-mail: peixt@ ***) Department of Stem Cell Biology Beijing Institute of Transfusion Medicine China

In recent years, with the increasingly further studies on embryonic stem cells and the recognition of the biologic characteristics of adult stem cells, it has been discovered that adult stem cells have another phenomenon of "Plasticity" in addition to the characteristics of strong poten- tial for self-renewal, proliferation and multi-differentiation, which brings us the hope for regenerative medicine —— renewing new organ or tissue cells to replace those damaged by injury or diseases. Although the mechanism of "Plastic- ity" and its application in the regenerative medicine are still in doubt, thorough exploration in these subjects would open up broad prospects for the use in cell and tissue engineering in the near future.

关键词：胚胎干细胞再生医学可塑性病理学

Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

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Science china(Life Sciences) 2004年第3期47卷 241-250页

作者： ZHANG Jie1,2. SHAN Qing1, MA Ping1, JIANG Yanming1, CHEN Peng1, WEN Jingxia1, ZHOU You1, QIAN Huanwen1 & PEI Xuetao2.1. beijing Institute of Radiation medicine,{2., china 2. beijing Institute of transfusion medicine,{2., china beijing Institute of Radiation medicine Beijing 100850 China beijing Institute of transfusion {2. Beijing 100850 China beijing Institute of Radiation medicine Beijing 100850 China beijing Institute of transfusion {2. Beijing 100850 China

Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bi-polar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 2., 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Elec-troretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas.

关键词： MSCs, retina, laser, differentiation.

Regulation of β-catenin Degradation by Tumor Suppressor APC

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Regulation of β-catenin Degradation by Tumor Suppressor APC

Pharmacokinetics of sifuvirtide, a novel anti-HIV-1 peptide, in monkeys and its inhibitory concentration in vitro1

2.08年全国生物化学与分子生物学学术大会

作者： Jun Yang (beijing Institute of transfusion {2.,beijing 1.0850,china)

APC (adenomatous polyposis coli) and β-catenin are key components in the Writ signaling transduction *** than 85% of colorectal cancers are initiated by APC mutation and about half of the remainder has β-catenin ***...

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Acta Pharmacologica Sinica 2005年第10期26卷 1274-1280页

作者： Shu-jia DAI~2. Gui-fang DOU~3, Xiao-hong QIAN~(4,5), Hai-feng SONG~4, Zhong-ming TANG~2. De-sheng LIU~2. Xiu-wen LIU~2. Liu-meng YANG~6, Yong-tang ZHENG~6, Qing LIANG~7 ~2.Laboratory of Metabolism of Biotechnology Derived Drugs, beijing Institute of Radiation medicine,{2. ~3 Laboratory of Drug Metabolism and Pharmacokinetics, beijing Institute of transfusion medicine,{2. ~5 Laboratories of Genomics and Proteomics, beijing Institute of Radiation medicine,{2. ~6 Laboratory of Molecular hnmunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 6502.3 ~7 FusoGen Pharmaceuticals Inc, Tianjin 300051, china LaboratoryofMetabolismofBiotechnologyDerivedDrugs BeijingInstituteofRadiationMedicineBeijing100850 LaboratoryofDrugMetabolismandPharmacokinetics BeijingInstituteofTransfusionMedicineBeijing100850

Aim: To study the pharmacokinetics of sifuvirtide, a novel anti-human immunodeficiency virus （HIV） peptide, in monkeys and to compare the inhibitory concentrations of sifuvirtide and enfuvirtide on HIV-1-infected-cell fusion. Methods: Monkeys received 1.2.mg/kg iv or sc of sifuvirtide. An on-line solid-phase extraction procedure combined with liquid chromatography tandem mass spectrometry （SPELC/MS/MS） was established and applied to determine the concentration of sifuvirtide in monkey plasma. A four-12.I iodinated peptide was used as an internal standard. Fifty percent inhibitory concentration (IC50) of sifuvirtide on cell fusion was determined by co-cultivation assay. Results: The assay was validated with good precision and accuracy. The calibration curve for sifuvirtide in plasma was linear over a range of 4.88-5000μg/L, with correlation coefficients above 0.992.. After iv or sc administration, the observed peak concentrations of sifuvirtide were 10 62.±2.886μg/L and 52.±191μg/L, and the terminal imination half-lives (T1/2. were 6.3±0.9 h and 5.5±1.0 h, respectively. After sc, Tmaxwas 0.2.-2., and the absolute bioavailability was 49%±13%. Sifuvirtide inhibited the syncytium formation between HIV-1 chronically infected cells and uninfected cells with an IC50of 0.33μg/L. Conclusion: An on-line SPE-LC/MS/MS approach was established for peptide pharmacokinetic studies. Sifuvirtide was rapidly absorbed subcutaneously into the blood circulation. The T1/2.f sifuvirtide was remarkably longer than that of its analog, enfuvirtide, reported in healthy monkeys and it conferred a long-term plasma concentration level which was higher than its IC50in vitro.

关键词： sifuvirtide pharmacokinetics HIV HIV fusion inhibitors enfuvirtide on-line solid-phase extraction macaque

Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro

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Progress in Natural Science:Materials International 2004年第1期 27-31+98页

作者： ZHENG Min 1,2. , WANG Dongmei 1, HOU Lingling 1, LI Haimin 1, XIE Chao 1, JIAO Wencang 1, BAI Cixian 1, WANG Yaping 2.and PEI Xuetao 1*(1. Department of Stem Cell Biology, beijing Institute of transfusion {2., beijing 1.0850, china 2. Institute of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, china) InstituteofBasicMedicalSciences ChongqingUniversityofMedicalSciencesChongqing400016China DepartmentofStemCellBiology BeijingInstituteofTransfusionMedicineBeijing100850China

Ascorbic acid (AA) induced differentiation of neural stem cells (NSCs) into dopaminergic (DAergic) neurons is reported. NSCs derived from rat mesencephalon were maintained and expanded in a defined medium containing mitogens of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Compared with the control, ascorbic acid treatment led to more DAergic neuronal differentiation as indicated by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT), which are specific markers of dopamine neurons. AA induction also enhanced expression of Nurr1 and Shh. PD98059, an inhibitor of mitogen activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway, could block AA induced Nurr1, TH and DAT mRNA expression. The results might suggest a new strategy to provide enough dopaminergic cells for the therapy of Parkinson’s disease (PD), and Nurr1 and ERK signaling pathway might participate in the AA induced DAergic differentiation.

关键词： neural stem cells differentiation Parkinson’s disease mitogen activated protein kinase(MAPK)/extracellular signal-regulated kinase (ERK) pathway signal transduction

Partial sequencing of 5' non-coding region of 7 HGV strains isolated from different areas of china

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World Journal of Gastroenterology 1999年第5期5卷 432-434页

作者： WANG Xing-Tai ZHUANG Hui SONG Hai-Bo Ll He-Min ZHANG Hua-Yuan and YU Yang(National Institute for the Control of bormceutical and BiologicalPriducts, beijing 100050, china)(Department of Microbiology, beijing Medical University, beijing100083, china)(L National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China Department of Virology Department of Microbiology Beijing Medical University Beijing 100083 China Laboratory for Clinical Diagnosis Hefei 230000 Anhui Province China

INTRODUCTIONAlthough sensitive tests for detection of known hepatitis viruses are available, the etiology of 10%-15% post-transfusion and community-acquired hepatitis cases has remained undefined. It suggests the exis... 详细信息

关键词： hepatitis G virus polymerase chain reaction nucleotide sequence RNA, viral

神经元限制性沉默因子在干细胞发育及分化中的作用

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生物化学与生物物理进展 2011年第9期38卷 791-796页

作者：张静李艳华裴雪涛军事医学科学院野战输血研究所全军干细胞与再生医学重点实验室北京100850

神经元限制性沉默因子NRSF/REST(neuron-restrictive silencer factor,NRSF;又称RE-1 silencing transcription factor,REST)是一种具有锌指结构的蛋白质,通过与特异的作用元件NRSE(neuron-restrictive silencer element,NRSE;又称Repressorelement,RE1)相结合,调节靶基因的转录.NRSF/REST通过与多种辅助因子的相互作用不同程度地影响靶基因的表达,其功能受到干扰则导致多种病理状态.近年来研究表明,NRSF/REST蛋白参与调控胚胎干细胞自我更新和全能性的维持,及干细胞的定向分化等多个生理过程.综述了最近NRSF/REST的研究进展,探讨其在胚胎干细胞自我更新、胚胎早期发育,以及干细胞向神经元、胰岛细胞分化中的作用.

关键词：神经元限制性沉默因子胚胎干细胞神经元胰岛细胞分化

葛根素引起急性溶血和肾功能衰竭的致病机制探讨

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中国新药与临床杂志 2003年第11期22卷 699-702页

作者：朱玉珍汪波马明信北京大学第一医院输血科北京100034 北京大学第一医院急诊科北京100034

目的 :探索葛根素引起急性溶血和肾功能衰竭 (肾衰 )的病理机制。方法 :作血液免疫学体外药物模拟抗球蛋白试验 (AGT)。以病人d 1,d 8及8mo的血样为标本 ,作直接抗球蛋白试验 (DAT)检测病人红细胞 (Pc)上的免疫复合物 ,行间接抗球蛋白试... 详细信息

目的 :探索葛根素引起急性溶血和肾功能衰竭 (肾衰 )的病理机制。方法 :作血液免疫学体外药物模拟抗球蛋白试验 (AGT)。以病人d 1,d 8及8mo的血样为标本 ,作直接抗球蛋白试验 (DAT)检测病人红细胞 (Pc)上的免疫复合物 ,行间接抗球蛋白试验 (IAT)检测O型供者红细胞 (Dc)与病人血清(Ps)、葛根素、补体孵育后在盐水介质及抗球蛋白血清 (AGS)中有否凝集或溶血。结果 :PcDAT阳性 ;IAT———孵育后的Dc在盐水介质中凝集 ,在IAT洗涤过程中部分溶血 ;在AGS中 ,Dc与抗广谱AGS、抗 C3的凝集强于抗 IgG。单纯药物孵育的Dc不凝集 ,不加葛根素的Ps及加入葛根素的AB型供者体血清红细胞 (Ds)不凝集 ;8mo后重复Pc和Ps试验 ,凝集全部消失。结论 :Ps中含有可活化补体的IgM类抗葛根素抗体 ,此为急性血管内溶血。

关键词：溶血肾功能衰竭急性抗原抗体反应葛根素

Determination of CS-055 by liquid chromatography /electrospray mass spectrometry in human and monkey plasmas

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Determination of CS-055 by liquid chromatography /electrospr...