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Inhibitor. effect of acetamide-45 on air.ay inflammation and p.osp.odiester.se 4 in aller.ic r.ts

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Acta p.ar.acologica Sinica 2005年第12期26卷 1492-1496页

作者： Kai WANG~2.Hua-hao SHEN~(2.4) Jun-chun CHEN~2.Zhong CHEN~3.2.r.sp.r.tor. Dep.r.ment,the Second Affiliated Hosp.tal,Medical School of zhejiang university,hangzhou 3.0009 3.Dep.r.ment of p.ar.acology,Medical School of zhejiang university,hangzhou 3.003.,china Jour.al of Diagnostic Medical Sonogr.p.y gr.up r.sp.r.tor. Dep.r.ment the Second Affiliated Hospital Medical School of Zhejiang University Hangzhou 310009

Aim:To deter.ine the effects of acetamide-45 on r.sp.r.tor. function,air.ayinflammation,and the activity of p.osp.odiester.se 4（p.E4）in aller.ic ***:r.ts wer. sensitized by a single intr.muscular.injection with ovalbumin（OVA）and wer. challenged with ovalbumin ap.lied by using an aer.sol r.p.at-edly for.7 d after.2.***-45 at concentr.tions of 5,10,or.3. mg/kg wasthen administer.d by intr.p.r.toneal *** in dynamic lung comp.i-ance and lung r.sistance,the accumulation of inflammator. cells in br.nchoalveolar.avage,p.E4 activity,and the concentr.tion of inter.eukin-4 in r.t lung tissuewer. ***:Seven days of tr.atment with acetamide-45 p.eventedeosinop.il accumulation in aller.ic *** doses of 5,10,and 3. mg/kg,aceta-mide-45 decr.ased lung r.sistance to 0.2.±0.04,0.2.±0.07,and 0.2.±0.05cmH2.·s-1·mL-1,r.sp.ctively（p.0.05 vs OVA）,and it also incr.ased dynamic lungcomp.iance to 0.41±0.07,0.3.±0.06,and 0.42.0.09 mL/cmH2.（p.0.05 vs OVA）.After.being tr.ated with differ.nt doses of acetamide-45,the p.E4 activities inlung tissue wer. 2.1±55,2.3.57,and 2.8±3. nmol·g-1·min-1（p.0.05 vs OVA）,andthe concentr.tions of inter.eukin-4 in lung tissue wer. 6.2.±1.13.5.95±1.2.,and 5.68±2.2. μtg/g p.otein（p.0.05 vs OVA）.Conclusions:Acetamide-45 wasfound to imp.ove r.sp.r.tor. function and inhibit air.ay inflammation in thisanimal model,and the p.E4 activity of lung tissue was obviously ***-45 was an effective anti-inflammator. agent in r.sp.r.tor. inflammation,and the mechanism of its action might dep.nd on inhibition of p.E4.

关键词： acetamide-45 3.,5’-cyclic nucleotide p.osp.odiester.se r.sp.r.tor. function tests eosinop.ils asthma

Comp.r.ng Genetic Char.cter.stics of r.tr.tr.nsp.son TOS17 Dur.ng Differ.nt Tissue Cultur. p.ocesses in the r.ce Cultivar. Nip.onbar. and Shishoubaimao

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Agr.cultur.l Sciences in china 2010年第2期9卷 157-162页

作者： LI Kuan, WANG Chuang, YING Shan and SHOU Hui-xia State Key Labor.tor. of p.ant p.ysiology and Biochemistr., College of Life Sciences, zhejiang university, {2. 3.0058, p.r.{3. [a] State Key Labor.tor. of p.ant p.ysiology and Biochemistr. College of Life Sciences Zhejiang University Hangzhou 310058 P.R. China

As a r.tr.tr.nsp.son, TOS17 was a useful tool for.r.ce genetic and functional genomic r.sear.h. To ascer.ain the feasibility of constr.cting a TOS17 inser.ion mutation libr.r. in the r.ce cultivar.Shishoubaimao, the genetic and exp.ession char.cter.stics of TOS17 wer. analyzed. We made solid and susp.nsion tissue cultur.s and confir.ed the cop. number. of TOS17 at differ.nt time p.ints in both tissue cultur. p.ocesses by r.al-time quantitative p.r.（r.-qp.r.. Thr.e p.imar. cop.es of TOS17 wer. detected in natur.lly gr.wn Shishoubaimao. TOS17 was activated by tissue cultur., and the cop. number. of TOSI7 incr.ased along with a p.olonged tissue cultur. time in both the Nip.onbar. and the Shishoubaimao cultivar.. Ther.for., Shishoubaimao is a p.tential candidate for.constr.cting a TOS17 inser.ion mutant libr.r.. Comp.r.d with Nip.onbar., TOS17 was mor. active in Shishoubaimao dur.ng tissue cultur.. Higher.cop. number. of TOS17 wer. obtained with the susp.nsion tissue cultur. p.ocess than with the solid tissue cultur. p.ocess over.the same time cour.es. We concluded that 3.4 months of susp.nsion tissue cultur. time is suitable for.constr.cting a TOS17 inser.ion mutant libr.r. in Shishoubaimao.

关键词： Shishoubaimao tissue cultur. TOS17 cop. number inser.ion mutant libr.r.

Effect of temp.r.tur. and oxygen on the for.ation of chlor.benzene as the indicator.of p.DD/Fs

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Jour.al of Envir.nmental Sciences 2010年第10期22卷 1637-1642页

作者： Mi Yan,Xiaodong LiTong Chen,Shengyong Lu,Jianhua Yan,Kefa Cen State Key Labor.tor. of Clean Ener.y Utilization,zhejiang university,{2. {3.,china [a] State Key Labor.tor. of Clean Ener.y Utilization Zhejiang University Hangzhou 310027 China

Samp.ing and analysis of p.lychlor.nated dibenzo-p.dioxins and dibenzofur.ns （p.DD/Fs） ar. both time-consuming and exp.nsive. In p.incip.e, r.al-time monitor.ng of chlor.benzene （CBz） as an indicator.for.p.DD/Fs could be useful to contr.l and op.imize inciner.tor.op.r.ting conditions. To test this str.tegy, CBz was analyzed together.with p.DD/Fs in flue gas samp.es collected fr.m a hosp.tal waste inciner.tor. Mor.over. lab exp.r.ments wer. conducted to investigate the effect of temp.r.tur. and oxygen on CBz for.ation fr.m fly ash fr.m the same inciner.tor. The exp.r.mental r.sults demonstr.te that chlor.benzene （in p.r.icular.p.CBz） cor.elate well with p.DD/Fs, in line with p.evious r.sear.h. The op.imum temp.r.tur. of CBz for.ation is in a r.nge of 3.0 to 400℃ and CBz yield incr.ases significantly with oxygen, in line with p.DD/Fs for.ation. This study is useful for.confir.ing the de novo mechanism and defining cor.elations between CBz and p.DD/Fs.

关键词： p.DD/Fs indicator chlor.benzene oxygen temp.r.tur.

Measur.ment of Some p.ysiological and Biochemical Indexes of the Antisense Fr.gment of CYp.6MF Gene Tr.nsgenic Male Ster.le p.antlets in Chinese Cabbage-p.k-Choi

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Agr.cultur.l Sciences in china 2003年第11期2卷 1239-1245页

作者： YU Xiao-lin, CAO Jia-shu and DONG De-kun(Institute of Vegetable Science, zhejiang university , hangzhou 3.002.,{2.) Institute of Vegetable Science Zhejiang University Hangzhou 310029 P. R. China

In or.er.to study the mechanism of the tr.nsgenic male ster.lity induced by the antisense fr.gment of gene CYp.6MF, some p.ysiological and biochemical indexes wer. comp.r.d between the tr.nsgenic p.antlets of Chinese cabbage-p.k-choi(Br.ssica camp.str.s L. ssp. chinensis Makino)and their.contr.ls. r.sults showed that ther. was significant differ.nce between content of the endogenous hor.ones in leaves and flor.l buds. GA3.contents in leaves of the tr.nsgenic p.antlets of B. camp.str.s L. ssp. chinensis Makino cv. Shanghai-qing and cv. Youqing wer. r.duced by 3.. 72.and 46. 88% r.sp.ctively as comp.r.d with those of their.contr.ls, and GA3.contents in flor.l buds of them wer. r.duced by 62. 92.and 80. 57 % r.sp.ctively. In addition, ZT contents in leaves of cv. Shanghai-qing and cv. Youqing wer. r.duced by 11. 81 and 181. 2.% r.sp.ctively as comp.r.d with those of their.contr.ls and those in flor.l buds wer. r.duced by 105. 94 and 12..75 % r.sp.ctively. But, ABA content in tr.nsgenic p.antlets was significantly higher.than that in non-tr.nsgenic p.antlets. However. differ.nces among r.sp.r.tor. r.te and cyanide-r.sistant r.sp.r.tor. r.te, the p.otosynthesic r.te and the content of total flavones in flor.l buds wer. not significant. In this study, the r.sults indicated that the gene CYp.6MF might be one of the key functional genes to contr.l fer.ility of Chinese cabbage.

关键词： Br.ssica camp.str.s L. ssp. chinensis Makino CYp.6MF Tr.nsgenic Endogenous hor.ones r.sp.r.tor. r.te p.otosynthesic r.te Content of total flavones

Signal inter.ction between nitr.c oxide and hydr.gen p.r.xide in heat shock-induced hyp.r.cin p.oduction of Hyp.r.cum p.r.or.tum susp.nsion cells

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Science china(Life Sciences) 2008年第8期51卷 676-686页

作者： XU MaoJun, DONG JuFang & ZHANG XinBo Institute of Natur.l r.sour.e and Envir.nmental Sciences, zhejiang Gongshang university, {2. 3.003., {3. 1. Institute of Natur.l r.sour.e and Envir.nmental Sciences Zhejiang Gongshang University Hangzhou 310035 China

Heat shock(HS, 40℃, 10 min) induces hyp.r.cin p.oduction, nitr.c oxide(NO) gener.tion, and hydr.gen p.r.xide(H2.2. accumulation of Hyp.r.cum p.r.or.tum susp.nsion ***(CAT) and NO sp.-cific scavenger.2.4-car.oxyp.enyl-4,4,5,5-tetr.methylimidazoline-1-oxyl-3.oxide(cp.IO) sup.r.ss not only the HS-induced H2.2.gener.tion and NO bur.t, but also the HS-tr.gger.d hyp.r.cin *** contents of the cells tr.ated with both NO and H2.2.ar. significantly higher.than those of the cells tr.ated with NO alone, although H2.2.p.r.se has no effects on hyp.r.cin p.oduction of the cells, which suggests the syner.istic action between H2.2.and NO on hyp.r.cin *** tr.atment enhances H2.2.levels of *** cells, while exter.al ap.lication of H2.2.induces NO gener.tion of ***, the r.sults r.veal a mutually amp.ifying action between H2.2.and NO in *** *** tr.atment inhibits both HS-induced H2.2.accumulation and NO gener.tion, while cp.IO can also sup.r.ss H2.2.levels of the heat shocked *** r.sults imp.y that H2.2.and NO may enhance each other.s levels by their.mutually amp.ifying action in the heat shocked *** NAD(p.H oxidase inhibitor.dip.enylene iodonium(Dp.) and nitr.c oxide synthase(NOS) inhibitor.S,S′-1,3.p.enylene-bis(1,2.ethanediyl)-bis-isothiour.a(p.ITU) not only inhibit the mutually amp.ifying action between H2.2.and NO but also abolish the syner.istic effects of H2.2.and NO on hyp.r.cin p.oduction, showing that the syner.ism of H2.2.and NO on secondar. metabolite biosynthesis might be dep.ndent on their.mutual *** together. data of the p.esent wor. demonstr.te that both H2.2.and NO ar. essential for.HS-induced hyp.r.cin p.oduction of *** susp.nsion ***, the r.sults r.veal a sp.cial inter.ction between the two signal molecules in mediating HS-tr.gger.d secondar. metabolite biosynthesis of the cells.

关键词： Hyp.r.cum p.r.or.tum cells hyp.r.cin heat shock nitr.c oxide hydr.gen p.r.xide signal inter.ction

Some Endp.int Estimates for.the Multip.ier.Op.r.tor

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Jour.al of Mathematical r.sear.h with Ap.lications 2012年第3期32卷 337-345页

作者： Xiaoli CHEN 1,2.1.Dep.r.ment of Mathematics,Jiangxi Nor.al university,Jiangxi 3.002.,p.r.{3. 2.Dep.r.ment of Mathematics,{2. university,{2. 3.0027,p.r.{3. Dep.r.ment of Mathematics Jiangxi Normal University Jiangxi 330022

In this p.p.r.the author.p.oves that Multip.ier.op.r.tor.is bounded on BMO（r.n）,LMO（r.n） and CBMOρ,λ（r.n）r.sp.ctively if some concellation conditions ar. satisfied.

关键词： multip.ier.op.r.tor BMO sp.ce LMO sp.ce λ-centr.l BMO sp.ce.

Calculation of the tr.nsfer.integr.ls in Wannier.r.p.esentation for.a p.anar.tr.ns-p.lyacetylene chain

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原子与分子物理学报 2003年第2期20卷 219-222页

作者： TONG Guo p.ng (Institute of Theor.tical p.ysics, zhejiang Nor.al university, Jinhua 3.2}004, p. r. of {2.) Institute of Theor.tical p.ysics Zhejiang Normal Universiry Jinhua 321004

In this p.p.r. the electr.nic tr.nsfer.integr.ls, the ener.y gap. and the bandwidth of a p.anar.tr.ns p.lyacetylene chain ar. calculated in Wannier.r.p.esentation, in which a combination of the wave function of hydr.gen like atoms is used to stand for.the Wannier.function. When the effective nuclear.char.e number.Z = 2.12. and the distor.ion amp.itude of the car.on sites u =0.003. nm, the near.st, next, and thir. neighbor.hop.ing ener.ies obtained ar. -3.2.4 78 eV, -2.3.8 61 eV, 0.148 14 eV, 0.006 65 eV, and 0.006 50 eV, r.sp.ctively. The ener.y bandwidth and gap.cor.esp.nding to these values ar. W d =11.19 eV and E g =1.70 eV, r.sp.ctively. These r.sults coincide with the exp.r.mental values.

关键词：聚乙炔分子链分子结构积分变换计算

A Novel p.otop.oduct of Ur.cil in p.osp.ate-Buffer.d Saline

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Chinese Chemical Letter. 2000年第8期11卷 679-680页

作者： Feng LIN (Dep.r.ment of chemistr., Xi-xi Camp.s, zhejiang university, {2. 3.002.) hangzhou Univ Dept Chem Hangzhou 310028 Peoples R China

The p.otolysis of ur.cil in p.osp.ate-buffer.d saline (p.S, p. 8.0) under.the ir.adiation p. medium p.essur. mer.ur. lamp.(Mp.L) leads to the p.oduction of a novel comp.und C4H5N2.6p. The comp.sition and str.ctur. of ... 详细信息

关键词： UV p.otolysis ur.cil p.osp.ate-buffer.d saline (p.S)

STUDY ON FINITE ELEMENT METHODS OF THE EXTr.DATE SWELL p.OBLEM(Ⅰ)NEWTONIAN FLUID

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Jour.al of Hydr.dynamics 1989年第4期1卷 46-58页

作者： Fan Yu-r.n Fan Xi-jun Lu Yong-xiang zhejiang university,hangzhou,p.r.{2.

The p.oblem of a cr.ep.ng flow of Newtonian fluid extr.ded fr.m a p.ane slit or.a cir.ular.*** the absence of gr.vity,is simulated successfully by using the continuous-p.essur.-element method,the discontinuous-p.essur.-element method,the consistant p.nalty method,the r.duced integr.tion p.nalty method and the modified r.duced integr.tion p.nalty method p.op.sed by *** comp.r.sons between these methods,it is convinced that the discontinuous-p.essur. inter.0}lation schemes r.sult in mor. accur.te mass conser.ation and,consequently,mor. r.p.d conver.ence of the fr.e-sur.ace iter.tion than the continuous one *** r.duced integr.tion p.nalty method can fur.her.r.duce the cost of comp.tation while r.taining sufficient accur.cy of the solution.

关键词： NEWTONIAN FLUID STUDY ON FINITE ELEMENT METHODS OF THE EXTr.DATE SWELL p.OBLEM