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Gene flow from transgenic oilseed rape (Brassica napus L.) to cruciferous weeds under mentor pollen inducement

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Progress in Natural Science:Materials International 2007年第11期17卷 1284-1289页

作者： Zhao Xiangxiang1,2. Luo Yuming1, Lu Weiping2. Qi Cunkou3. Pu Huiming3.and Wang Youping2.1. Biotechnology Key Laboratory of Eco-agriculture in Hongze Lake Region, Jiangsu Province, Huaiyin Normal College, Huai'an 2.3.01, china 2. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 2.5009, china 3. {2. of Industrial Crops, Jiangsu academy of Agricultural sciences, Nanjing 2.0014, china) Biotechnology Key Laboratory of Eco-Agriculture in Hongze Lake Region Jiangsu Province Huaiyin Normal College Huai'an 223001 China Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China

The alien gene flow between genetically modified glyphosate-resistant rapeseed variety Q3.(Brassica napus L.) and four cruciferous weeds was studied under mentor pollen inducement. The results showed that when Thlaspi arvense L., Capsella bursa-pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser were pollinated with mentor pollen, the mixed Q3.and the weed, pollen grains aggregated largely and germinated quickly, and the numbers of pollen tubes penetrating into the style and the ovary were greatly increased as compared with corresponding self-pollination groups. Twenty four to forty eight hours after pollination, several pollen tubes were observed to penetrate into the ovule via micropyle in each mentor combination. However, when the mentor progenies were analyzed by PCR, all of them showed negative for the Q3.herbicide-resistant gene. Collectively, these results indicated that crossing between T. arvense, C. bursa-pastoris, C. hirsuta, R. palustris (as female) and Q3.(as male) was highly incompatible and the herbicide-resistant gene could not flow from Q3.to these four weeds.

关键词： herbicide-resistant rapeseed cruciferous weeds mentor pollination aniline blue fluorescence gene flow

Possible Role of Histone H1 in the Regulation of Furin-dependent Proprotein Processing

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 173-180页

作者： Jinbo HAN~1 Jianxin GU~2.Chengwu CHI~(1,3.) 1 institute of Biochemistry and Cell Biology,chinese {2. of sciences,Graduate School of the chinese academy of sciences,Shanghai 2.003.,china 2.Gene Research Center,Medical Center of Fudan University,Shanghai 2.003.0}china 3.institute of Protein Research,Tongji University,Shanghai 2.0092.china institute of Biochemistry and Cell Biology Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China.

Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitorsof furin,a mammalian proprotein ***,its role in the regulation of furin-dependent proproteinprocessing remains *** we report that histone H1 efficiently blocks furin-dependent pro-yonWillebrand factor（pro-vWF）processing in a dose-dependent *** and immunof-luorescence studies confirmed that histone H1 could interact with furin,and the interaction mainly took placeon the cell *** noted that histone H1 was released from cells undergoing necrosis and apoptosisinduced by *** findings suggested that histone H1 might be involved in extracellular and/or intracellu-lar furin regulation.

关键词： furin histone H1 pro-vWF proprotein processing

The dynamical properties of Rydberg hydrogen atom near a metal surface

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Science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 667-675页

作者： GE Meihua1, ZHANG Yanhui1, WANG Dehua2. DU Mengli3.& LIN Shenglu1 1. College of Physics and Electronics, Shandong Normal University, Jinan 2.0014, china 2. Department of Mathematics and Physics, Shandong institute of Architecture and Engineering, Jinan 2.0014, china 3. institute of Theoretical Physics, chinese academy of sciences, Beijing 100080, china College of Physics and Electronics Shandong Normal University Jinan China Department of Mathematics and Physics Shandong Institute of Architecture and Engineering Jinan China institute of Theoretical Physics Chinese Academy of Sciences Beijing China

The dynamical properties of Rydberg hydrogen atom near a metal surface are presented by using the methods of phase space analysis and closed orbit theory. Transforming the coordinates of the Hamiltonian, we find that the phase space of the system is divided into vibrational and rotational region. Both the Poincaré surface of section and the closed orbit theory verify the same conclusion clearly. In this paper we choose the atomic principal quantum number as n = 2.. The dynamical character of the exited hydrogen atom depends sensitively on the atom-surface distance d. When d is sufficiently large, the atom-surface potential can be expressed by the traditional van der Waals force and the system is integrable. When d becomes smaller, there exists a critical value dc. For d > dc, the system is near-integrable and the motion is regular. While chaotic motion appears for d < dc, and the system tends to be non-integrable. The trajectories become unstable and the electron might be captured onto the metal surface.

关键词： Rydberg hydrogen atom, van der Waals interaction, Poincaré surface of section, closed orbit theory, chaos.

Relative importance of water, energy, and heterogeneity in determining regional pteridophyte and seed plant richness in china

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Journal of Systematics and Evolution 2011年第2期49卷 95-107页

作者： 1,2.3.Sheng-Bin CHEN § 1 Gao-Ming JIANG 3.Zhi-Yun OUYANG 3.Wei-Hua XU 3.Yi XIAO 1 (State Key Laboratory of Vegetation and Environmental Change, institute of Botany, chinese academy of sciences, Beijing 100093.{2.) 2.(Graduate University of the chinese academy of sciences, Beijing 100049,{2.) 3.(State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental sciences, chinese academy of sciences, Beijing 100085,{2.) (State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate University of the chinese academy of sciences Beijing 100049 China) (State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China)

Environmental variables, such as ambient energy, water availability, and environmental heterogeneity have been frequently proposed to account for species diversity gradients. How taxon-specific functional traits define large-scale richness gradients is a fundamental issue in understanding spatial patterns of species diversity, but has not been well documented. Using a large dataset on the regional flora from china, we examine the contrast spatial patterns and environmental determinants between pteridophytes and seed plants which differ in dispersal capacity and environmental requirements. Pteridophyte richness shows more pronounced spatial variation and stronger environmental associations than seed plant richness. Water availability generally accounts for more spatial variance in species richness of pteridophytes and seed plants than energy and heterogeneity do, especially for pteridophytes which have high dependence on moist and shady environments. Thus, pteridophyte richness is disproportionally affected by water-related variables; this in turn results in a higher proportion of pteridophytes in regional vascular plant floras (pteridophyte proportion) in wet regions. Most of the variance in seed plant richness, pteridophyte richness, and pteridophyte proportion explained by energy is included in variation that water and heterogeneity account for, indicating the redundancy of energy in the study extent. However, heterogeneity is more important for determining seed plant distributions. Pteridophyte and seed plant richness is strongly correlated, even after the environmental effects have been removed, implying functional linkages between them. Our study highlights the importance of incorporating biological traits of different taxonomic groups into the studies of macroecology and global change biology.

关键词： habitat heterogeneity pteridophytes seed plants species richness water-energy hypothesis.

An analysis on the evolvement processes of lake eutrophication and their characteristics of the typical lakes in the middle and lower reaches of Yangtze River

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chinese Science Bulletin 2006年第13期51卷 1603-1613页

作者： CHENG Xiaoying1,2.3.& LI Shijie1 1. Nanjing institute of Geography & Limnology, chinese academy of sciences, Nanjing 2.0008,{2. 2. The Key Laboratory of Industrial Biotechnology, Ministry of Educa- tion, Southern Yangtze University, Wuxi 2.403.,{2. 3. School of Biotechnology, Southern Yangtze University, Wuxi 2.403.,{2. Nanjing institute of Geography & Limnology Chinese Academy of Sciences Nanjing China The Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze University Wuxi China School of Biotechnology Southern Yangtze University Wuxi China

According to the study on the aquatic environment of the typical lakes in the middle and lower reaches of Yangtze River, lake eutrophication is a process in which the lake ecosystem degenerates, resulting in the deterioration of the water quality due to the enrichment of the nutrients. Moreover, the succession of lake ecosystem and change of water quality both couple well with the evolvement of tro- phic state. Then the conceptual model is mentioned and ten lake states are classified. Based on these, the authors analyzed the eutrophication processes of the typical lakes in the middle and lower reaches of Yangtze River. The large lakes with taking in - send- ing out water typical of Poyang Lake evolve from State 1 before the 1980s into State 2.after the 1980s, the large and medium lakes typical of Taihu Lake evolve from State 1-2.in the 1960s into State 8 in the 1980s then into State 9 in the 1990s, the medium and small shallow lakes typical of Honghu Lake evolve from State 1 in the 1960s into State 2.after the 1980s and the small city (suburban) lakes typical of Donghu Lake evolve from State 2.in the 1960s into State 9 after the 1970s. Further, the 1980s is the key con- version period of the lakes of various types typical of Poyang Lake, Honghu Lake and Taihu Lake con- verting into mesotrophic state, the 1990s is that of Taihu Lake into eutrophic state and the 1970s is thatof the small city (suburban) lakes typical of Donghu Lake into eutrophic state. The series of nutrient evolvement of different typical lakes are similar, but the processes are not synchronous, which is related to the various human and natural driving mechanisms in lake basins.

关键词：长江湖泊富营养化概念模型转变时期驱动机制

Re-Os age for molybdenite from the Gangdese porphyry copper belt on Tibetan plateau: Implication for geody- namic setting and duration of the Cu mineralization

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Science china Earth sciences 2004年第3期47卷 221-231页

作者： HOU Zengqian1, QU Xiaoming1, WANG Shuxian2. DU Andao2. GAO Yongfeng3.& HUANG Wei4 1. institute of Mineral Resources, {3. {2. of Geological sciences, Beijing 10003., china 2. National Center of Geoanalysis, {3. {2. of Geological sciences, Beijing 10003., china 3. Shijiazhuang College of Economy, Shijiazhuang 056000, china 4. Xizang Bureau of Geology and Mineral Resources, Lhasa 850000, china institute of Mineral Resources Chinese Academy of Geological Sci. Beijing 100037 China National Center of Geoanalysis Chinese Academy of Geological Sci. Beijing 100037 China Shijiazhuang College of Economy Shijiazhuang 056000 China Xizang Bureau of Geol./Mineral Rsrc. Lhasa 850000 China

The Gangdese porphyry copper belt consists of one large and five middle-small deposits in addition to dozens of ore-bearing porphyry bodies. The belt trends 3.0 km long along the EW-striking Gangdese batholith, and locally occurs as a string of beads extending about 50 km within the SN-trending rifting zones (grabens) on the Tibetan plateau. Monzonitic granite- porphyry and quartz monzonitic porphyry, as dominant host rocks, are shoshonitic and potassic calc-alkaline. Available dating data indicate that the ages of the shallow-level emplacement for these porphyries vary from 10 Ma to 18 Ma, which are identical to that of associated potassic calc-alkaline volcanic rocks (10—15 Ma) and mafic dykes (13.18 Ma). The timing and duration of Cu mineralization events are constrained by Re-Os ages for molybdenites from three porphyry copper deposits in the Gangdese porphyry copper belt. Five molybdenite samples from the Nanmu deposit yielded an 187Re-187Os isochron with a highly pre-cise age of (14.76?.2.) Ma; six molybdenites from the Chongjiang deposit gave an isochron age of (14.04?.16) Ma. Re-Os model ages for two molybdenite samples from Lakang抏 deposit vary from 13.5 Ma to 13.6 Ma, which are basically identical to isochron ages mentioned above. All the thirteen samples from these three deposits yielded a linear array in the isochron diagram with a higher correlation coefficient of 0.99719 and an isochron age of (14.3.?.2.) Ma (1s error), suggesting an coeval event of the Cu mineralization and a shared source of ore materials for the Gangdese porphyry copper belt. Compared with the longer-lived felsic magmatic-hydrothermal system, the Cu mineralization is a relatively short event with duration of about 0.5 Ma, and usu-ally occurs in the later-stage of the complicate magmatic system. The emplacement age of the Gangdese porphyries indicates that they intruded after the rapid rising of the Gangdese Mountains at 2.—2. Ma, and formed in a post-collision crustal ex-tension env

关键词： porphyry copper deposit, Re-Os age, Gangdese, Tibetan plateau.

A three-dimensional variational ocean data assimilation system:Scheme and preliminary results

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Science china Earth sciences 2006年第11期49卷 1212-1222页

作者： ZHU Jiang1,2. ZHOU Guangqing1, YAN Changxiang1, FU Weiwei1 & YOU Xiaobao1,3.1. International Center for Climate and Environment sciences, institute of Atmospheric Physics,{2., Beijing 10002., china 2. Jiangsu Key Laboratory of Meteorological Disaster, Nanjing University of Information Science and Technology, Nanjing 2.0044, china 3. Beijing institute of Applied Meteorology, Beijing 10002., china International Center for Climate and Environment sciences Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China Jiangsu Key Laboratory of Meteorological Disaster Nanjing University of Information Science and Technology Nanjing China Beijing institute of Applied Meteorology Beijing China

A new 3.VAR-based Ocean Variational Analysis System (OVALS) is developed. OVALS is capable of assimilating in situ sea water temperature and salinity observations and satellite altimetry data. As a component of OVALS, a new variational scheme is proposed to assimilate the sea surface height data. This scheme considers both the vertical correlation of background errors and the nonlinear temperature-salinity relationship which is derived from the generalization of the linear balance constraints to the nonlinear in the 3.VAR. By this scheme, the model temperature and salinity fields are directly adjusted from the altimetry data. Additionally, OVALS can assimilate the temperature and salinity profiles from the ARGO floats which have been implemented in recent years and some temperature and salinity data such as from expendable bathythermograph, moored ocean buoys, etc. A 2.-year assimilation experiment is carried out by using OVALS and the Tropical Pacific circulation model. The results show that the assimilation system may effectively improve the estimations of temperature and salinity by assimilating all kinds of observations. Moreover, the root mean square errors of temperature and salinity in the upper depth less than 42. m reach 0.63. and 0.3. psu.

关键词： data assimilation, 3.VAR, sea surface height, ARGO floats.

Expression of Monomeric Insulin Precursor in Pichia pastoris and Its Conversion into Monomeric B2. Lys Destripeptide Insulin by Tryptic Hydrolysis

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3. Jian FEI~2.Da-Fu CUI~1 You-Shang ZHANG~1 ~1institute of Biochemistry and Cell Biology,and ~2.odel Organism Research Center,Shanghai institutes for Biologicol {2., chinese academy of sciences,Shanghai 2.003.,china ~3.raduate School of the chinese academy of sciences,Shanghai 2.003.,china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Graduate School of the chinese academy of sciences Shanghai 2.003. China Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Monomeric B2. Lys destripeptide insulin (B2. Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B2. Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B2. Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B2. Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 2.0 mg per liter of culture,and the overall yield ofpurified B2. Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B2. Lys DTrI asdetermined by the mouse convulsion assay was 2. U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B2. Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

The optical near-field speckles and their first order statistics on the basis of the integral equations of electromagnetic field

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Science china(Physics,Mechanics & Astronomy) 2004年第3期47卷 365-380页

作者： CHENG Chuanfu1, 2. SONG Hongsheng2. LIU Chunxiang2. REN Xiaorong2. ZHANG Ningyu3. TENG Shuyun1 & XU Zhizhan1 1. Shanghai institute of Optics and Fine Mechanics, chinese academy of sciences, P. O. Box 800-2.1, Shanghai 2.1800,{2. 2. Department of Physics, Shandong Normal University, Jinan 2.0014,{2. 3. Shandong institute of Architecture and Engineering, Jinan 2.0014,{2. Shanghai institute of Optics and Fine Mechanicschinese {2. of {3.Shanghaichina Department of PhysicsShandong Normal UniversityJinanchina Shandong institute of Architecture and EngineeringJinanchina

From Helmholtz equation of the harmonic electromagnetic waves, the integral equations of the light field at the medium boundaries are obtained by use of the Green's theorem and are discretized into linear equation set with the values of the light field and its derivative as the unknowns. On solving the linear equation set, we realize the rigorous computations of the light fields at the boundaries. Then the intensities of the light waves scattered by the random self-affine fractal surfaces in the optical near-field are calculated, and the propagation characteristics, the evolutions of the contrast and the intensity probability density function of the near-field speckles are studied in detail. The near-field speckles are much different from the conventional speckles in the diffraction regions and in the imaging systems. There are obvious local fluctuations in the intensity distributions of the near-field speckles and such fluctuations disappear after propagating a distance of one wavelength from the medium surfaces. For the random surfaces with smaller lateral correlation lengths, the speckle contrasts approach the saturation values and the speckle fields approach Gaussian distribution within the near-field, while for the random surfaces with larger lateral correlation lengths, such evolutions become comparatively slow.

关键词： speckle, near-field optics, first order statistics, Green's function.