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Journal of Environmental sciences 2000年第4期12卷 463-468页

作者： DONG Yun\|she 1,2.3., Dieter Scharffe 2. Manfred Domroes 3. QI Yu chun 1, ZHANG Shen 1 (1. Institute of Geographic sciences and Natural Resources Research,{3.,{2., china E\|mail: dongys@*** 2. M 中科院地理科学与资源所北京 1 0 0 1 0 Max- Planck-Institute of Chemistry 德国 Mainz 550 2 0 Mainz University 德国 Mainz 550 99

An enclosed chamber technique was used to measure N 2. emissions from intensively agricultural soils of the North china Plain during the periods of 1995—1996 and 1997—1998, to reflect distinct components of winter wheat and summer maize growing seasons. The results showed that the continuous application of fertilizer in agricultural soils increased N\-2. emissions by a factor of 2..1—2..1, the calculated annual chemical N fertilizer\|transformed N\-2.\|N emissions was 0.67%. Our results indicated that the application of organic manure also had a significant influence on soil N 2. emissions, which combined with the use of chemical N increased about 2.% in a year. It was calculated that there were about 0.11% N of organic manure transformed as N 2. N. Annual mean N 2. emission from our study area of fertilized soils was estimated to be 57.1 μgN 2./(m 2.h). A weak correlation was also found between N 2. emissions and soil available nitrogen content NH + 4.

关键词： N_2. emissions the North china Plain agricultural soils chemical nitrogen organic manure

High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2., Libin DENG1#, and Changqing ZENG1,2. The Institute of Genetic and Developmental Biology, the chinese academy of sciences,{3.,{2. 2.3. Genomics Institute, the chinese academy of sciences, beijing 1013.0,{2. The Institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing Genomics Institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 2.–3. bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3.were genotyped in a sample of 90 individuals, 4792.sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

Simulating the impacts of climate variation and land-cover changes on basin hydrology: A case study of the Suomo basin

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Science china Earth sciences 2005年第9期48卷 1501-1509页

作者： CHEN Junfeng1,2. LI Xiubin2.& ZHANG Ming2.1. Institute of Remote Sensing & GIS, Peking University, {3. 100871, china 2. Institute of Geographical {2. and Natural Resources Research, chinese academy of sciences, beijing 100101, china 1. Institute of Remote Sensing & GIS Peking University 100871 Beijing China 2. Institute of Geographical sciences and Natural Resources Research Chinese Academy of Sciences 100101 Beijing Chin

Impacts of land cover changes on watershed hydrology have been a long-term academic concern with acute dispute. But little attention has been paid to such effects on mesoscale river basins, where the society has a closer link to river hydrology. The present study focuses on a mesoscale river basin, the Suomo Basin that is located on the upper reaches of the Yangtze River. Land covers in the basin in the years 1970, 1986 and 1999 were mapped. A lumped hydrologic model, CHARM, and a distributed hydrologic model, SWAT, were used to model the impacts of both land-cover change and climate variation on river runoff during the past four decades. The results show that the contribution of climate variation to the change of runoff regime makes up 60%―80%, while that of land cover changes only 2.%.

关键词： climate variation, land-cover changes, Suomo Basin, CHARM model, SWAT model.

The role of aerial algae in the formation of the landscape of the Yunnan Stone Forest,Yunnan Province, china

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Science china Earth sciences 2004年第9期47卷 846-864页

作者： TIAN Youping1,3. ZHANG Jie2. SONG Linhua1 & BAO Haosheng2.1. Institute of Geographic sciences and Natural Resources Research,{3.,{2., china 2. Department of Geography, Nanjing University, Nanjing 2.0093. china 3. School of Earth sciences and Resources, china University of Geosciences, beijing 100083. china Institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Department of Geography Nanjing University Nanjing 210093 China School of Earth sciences and Resources China University of Geosciences Beijing 100083 China

Aerial algae on the surface of carbonate rocks at the Stone Forest, Shilin County, Yunnan Province, china, and their bioerosion were investigated in the field and studied in the laboratory in detail. Through the observation, identification and statistics of more than one hun- dred algal samples and rock samples with the optical microscopes (stereomicroscope, biological microscope) and the scanning electronic microscope (SEM), the relationships between erosional forms on the surface of the Stone Forest and algae and/or algal communities and the genetic mechanism for the formation of erosional forms were analyzed. It is suggested that aerial algae play an active role in bioerosive processes that may affect the formation of karst erosional forms. These effects include both direct and indirect ones. The direct effect is the initiative control (algal shape-controlling role) of algae on the formation of karst forms of various scales, mostly mi- cro-scale (3.) and minor-scale (10-3.10-1m) erosional forms. The algal shape-controlling roles can be divided into the algal individual shape-controlling role and the algal community shape-controlling role. The former mostly controls the formation of micro-scale erosional forms, while the latter mostly controls the formation of micro-scale and smaller minor-scale erosional forms. The indirect effect refers to the promoting role of algae in the formation of karst forms, which may affect the formation of karst forms of all types and scales. The bioerosion of algae accelerates the weathering process of the whole Stone Forest karst landforms.

关键词： aerial algae, bioerosion, the Stone Forest, Yunnan Province, china.

Patterns of platinum-group elements in mantle peridotite, granulite xenoliths and basalt in Hannuoba

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chinese Science Bulletin 1999年第18期44卷 1676-1681页

作者： CHU Xuelei, LI Xiaolin, XU Jiuhua and LIU Jianming1. The Research Center of Mineral Resources Exploration , chinese academy of sciences , beijing 100101,{2. 2. Institute of Geology , chinese academy of sciences , beijing 10002.,{2. 3. Institute of High Energy Physics , chinese academy of sciences , beijing 100817 ,{2. 4. beijing University of Science and Engineering , beijing 100083.{2. Corresponding author The Research Center of Mineral Resources Exploration Chinese Academy of Sciences Beijing China Institute of Geology Chinese Academy of Sciences Beijing China Institute of High Energy Physics Chinese Academy of Sciences Beijing China beijing University of Science and Engineering Beijing China

Neutron activation analysis after preconcentration of nickel sulfide fire assay was used in measurement of Ru, Ph, Pd, Os, Ir, Pt (i.e. PGE elements) and Au concentrations for basalt, mantle and granulite xenoliths in Hannuoba. The chondrite- and primitive mantle-normalized PGE patterns generally exhibit flat (for Iherzolite), negative slope (for harzburgite),

关键词： mantle xenolith granulite xenolith basalt platinum-group element Hannuoba.

An integrated decision method for prediction of tropical cyclone movement by using genetic algorithm

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Science china Earth sciences 2005年第3期48卷 429-440页

作者： YANG Yuanqin1 & WANG Jizhi2.1. National Meteorological Center, beijing 100081,china 2. {2. academy of Meteorological sciences, beijing 100081, china 1. National Meteorological Center 100081 Beijing China 2. chinese academy of Meteorological {2. 100081 Beijing China

In this study an integrated decision method for the multi-prediction results of tropical cyclone movements by using genetic algorithm (GA) has been adopted. A genetic learning artifi- cial neural network (GLANN) as a learning mechanism for track prediction was developed through training with the huge samples of typhoon tracks from 1884 to 2.02. The prediction of typhoon motion is to consider how to suit the dynamic change of environment, where typhoon passed through. The neural network structure is described by GA. A fitness function for choice of GA is given to approach the maximum of fitness (the minimum of the errors). Parallel comparing analyses for prediction abilities of unusual typhoon tracks in recent years by GLANN with multi-forecast methods were conducted. More types of cases were employed in the comparing analyses, which consist of the typhoon, Winnie, No.11, in 1997, characterized by a long life over the ocean and in the mainland with severe effect on china, the 10 unusual typhoon tracks in the 1990s, the tracks with landfall and affected action in 1998 and the typhoons born in the Northwestern Pacific and the South china Sea in 2.00, including the 2.—48 h forecast, etc. The results indicated that for prediction ability of typhoon tracks, specially for unusual typhoons, GA decision-making developed in this study is superior to the others.

关键词： genetic algorithm, integrated decision, prediction of tropical cyclone.

Formal Verification under Unknown Constraints

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Wuhan University Journal of Natural sciences 2005年第1期10卷 43-46页

作者： LI Guang-hui 1,2.3., LI Xiao-wei 2.3.. School of Information Engineering, Zhejiang Forestry College, Hangzhou 3.13.0, Zhejiang, china 2. Institute of Computing Technology, chinese academy of sciences, {2. 100080, china 3. Graduate School of the chinese academy of sciences, {2. 10003., china School of Information Engineering Zhejiang Forestry College Hangzhou 311300 Zhejiang China

We present a formal method of verifying designs with unknown constraints (e.g., black boxes) using Boolean satisfiability (SAT). This method is based on a new encoding scheme of unknown constraints, and solves the corresponding conjunctive normal form (CNF) ***,this method can avoid the potential memory explosion, which the binary decision diagram (BDD) based techniques maybe suffer from, thus it has the capacity of verifying large designs. Experimental results demonstrate the efficiency and feasibility of the proposed method.

关键词： formal verification unknown constraints black box Boolean satisfiability Boolean comparison

Analysis of tandem repeats in the genome of chinese shrimp Fenneropenaeus chinensis

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chinese Science Bulletin 2005年第14期50卷 1462-1469页

作者： KONG Jie1 & GAO Huan2.3.1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, chinese academy of Fishery {2., Qingdao 2.6071, china 2. Institute of Oceanology, chinese academy of sciences, Qingdao 2.6071, china 3. The Graduate School of the chinese academy of sciences, beijing 10003., china Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheris Research Institute Chinese Academy of Fishery Sciences Qingdao China Institute of Oceanology Chinese Academy of Sciences Qingdao China The Graduate School of the chinese academy of sciences Beijing China

Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2.59 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 2..6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2. of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42.. In decreasing order, the 2. most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (2.4), AAT (92., A (56), AAG (2.), ATC (2.), ATAG (2.), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them.

关键词：虾遗传特性遗传基因 DNA

The effects of K^+ growth conditions on the accumulation of cesium by the bacterium Thermus sp. TibetanG6

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Science china(Life sciences) 2006年第2期49卷 123-129页

作者： WANG Hailei1,2.3. KONG Fanjing1,2.3.& ZHENG Mianping1,2.3.1. R&D Center for Saline Lake and Epithermal Deposits, {3. academy of Geological {2., beijing 10003., china 2. Key Open Laboratory of Saline Lake Resources and Environment, {3. academy of Geological {2., beijing 10003., china 3. Institute of Mineral Resources, {3. academy of Geological {2., beijing 10003., china R&D Center for Saline Lake Epithermal Deposits Chinese Academy of Geological Sciences Beijing 100037 China Key Open Laboratory of Saline Lake Resources Environment Chinese Academy of Geological Sciences Beijing 100037 China Institute of Mineral Resources Chinese Academy of Geological Sciences Beijing 100037 China

The accumulation of cesium by the bacterium Thermus sp. TibetanG6 was examined under different K+ growth conditions. The effects of external pH and Na+ on the accumulation of ce-sium were also studied, and the mechanism involved was discussed. K+ regimes played an important role in the accumulation of cesium by the strain TibetanG6. The quantity of cesium accumulated (2. h) was much higher in K+-deficient regime than that in K+-sufficient regime. The pH and Na+ had different effects on the accumulation of cesium in the two K+ regimes. IR spectra analyses indicated that the biosorption is a process of homeostasis with cesium initially accumulated on the cell wall.

关键词： Thermus sp. TibetanG6, cesium, bioaccumulation, K+-growth condition, pH, sodium, IR spectroscopy.

Crustal structure of Gondwana-and Yangtze-typed blocks:An example by wide-angle seismic profile from Menglian to Malong in western Yunnan

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Science china Earth sciences 2005年第11期48卷 1828-1836页

作者： ZHANG Zhongjie1, BAI Zhiming1, WANG Chunyong2. TENG Jiwen1, Lü Qingtian3. LI Jiliang1, SUN Shanxue1 & WANG Xinzheng1 1. Institute of Geology and Geophysics, chinese academy of sciences, beijing 10002.,{2. 2. Institute of Geophysics, chinese Seismological Bureau, beijing 100081,{2. 3. Institute of Deposition Resource, chinese academy of Geological sciences, beijing 10003.,{2. 1. Institute of Geology and Geophysics Chinese Academy of Sciences 100029 Beijing China 2. Institute of Geophysics Chinese Seismological Bureau 100081 Beijing China 3. Institute of Deposition Resource Chinese Academy of Geological Sciences 100037 Beijing China

The wide-angle seismic profile between Menglian and Malong crosses the Baoshan block (Gondwana-typed), and Simao and southwestern Yangtze blocks (Yangtze-typed). By in-terpreting the wide-angle seismic data, we obtained the seismic crust/upper mantle structure of P-wave velocities together with the seismic reflections of these three blocks, Changning- Menglian and Mojiang suture zones among the mentioned three blocks. Our interpreting results demonstrate that the P-wave crustal velocity of Simao block is slower than that of Baoshan and southwestern Yangtze block and the crustal thickness gradually thickens from the Baoshan block, Simao to southwestern Yangtze block. Crustal reflection patterns of these three blocks have dis-tinct differences too. For the Gondwana-typed blocks, seismic reflections in the upper crust are well developed while in middle-lower crust they are very weak. The crustal reflections in the Yangtze block are very well developed. The crustal reflection patterns in Simao and southwest-ern Yangtze blocks are distinguishable. The average thickness of the crust in the studied area is about 40 km. And we make some discussions on the crustal thickening model of the three blocks in western Yunnan and tectonic setting of seismic developing and interaction of Gondwana and Yangtze blocks.

关键词： Gondwana blocks,Yangtze blocks,wide-angle profile,crustal structure,tectonic setting