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Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers

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chinese Science Bulletin 1999年第4期44卷 348-351页

作者： JIANG Shuye, CHEN Qifeng and FANG Xuanjun Biotechnology Research Center, chinese academy of Agricultural Science, {2. 100081,{3. Institute of Genetics and Crop Breeding, Fujian Agricultural University, Fuzhou 3.0002.{3. Corresponding author Biotechnology Research Center Chinese Academy of Agricultural Science Beijing China Institute of Genetics and Crop Breeding Fujian Agricultural University Fuzhou China

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility *** populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83.primers amplified the same banding patterns, and 3. primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 3. primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 2.%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.

关键词： photo-period sensitive genie male sterile genes cDNA-RAPD differential display analysis.

From deep basin gas to diagenetic trap—An example from the Yulin Gas Field in the Ordos Basin

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From deep basin gas to diagenetic trap—An example from the ...

中国科学院地质与地球物理研究所2.07学术年会

作者： ZHANG LiuPing~(1+),LUO XiaoRong~1,MA XinHua~2.CHEN MengJin~2.& WU MingHui~1 1 Key Laboratory of Petroleum Resource,chinese {3. of {2.,beijing 10002.,china 2.Research Institute of Petroleum Exploration and Development,Petrochina,beijing 100083.china

In the updip portion of the Yulin Gas Field in the Ordos Basin,there are no any structural,stratigraphic and sedimentary lithologic *** thin-section petrography,ultraviolet fluorescence microscopy, micro-thermometry and Raman microspectrometry,this paper finds out the diagenetic trap and studies the diagenetic history of this *** was revealed that three phases of diagenesis and hydrocarbon charging happened in late Triassic,late Jurassic and the end of early Cretaceous *** the first two phases,acid geofluid entered the reservoir and caused dissolution and *** the porosities had been increased,further compaction accompanying re-subsidence resulted in tight sandstone and *** the end of the early Cretaceous,bulk of gas migrated into the tight ***,however,kept on in the updip portion of this field due to low gas saturation and formed the diagenetic *** mechanism for gas accumulation was changed from deep basin gas to diagenetic trap,which offers a sealing condition that can retain gas for much longer time.

关键词： natural gas deep basin gas tight reservoir diagenetic trap Ordos Basin

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西藏扎布耶盐湖太阳池的建造与运行试验

太阳能学报 2010年第2期31卷 163-167页

作者：黄维农王学魁孙之南乜贞沙作良天津科技大学海洋科学与工程学院天津300457 西藏扎布耶锂业高科技有限公司拉萨850000 中国地质科学院矿产资源研究所北京100037 国土资源部盐湖与环境开放研究实验室北京100037

介绍了西藏扎布耶盐湖面积为3.88m^2.阳池的建造与运行试验,获得了扎布耶盐湖太阳池运行过程中的温度和盐度分布情况,找到了不同时期简单实用的梯度层构建方法。基于当地条件,扎布耶盐湖太阳池的构建费用低,使用淡水量小,梯度层薄(3.～... 详细信息

介绍了西藏扎布耶盐湖面积为3.88m^2.阳池的建造与运行试验,获得了扎布耶盐湖太阳池运行过程中的温度和盐度分布情况,找到了不同时期简单实用的梯度层构建方法。基于当地条件,扎布耶盐湖太阳池的构建费用低,使用淡水量小,梯度层薄(3.～50cm);下对流层(LCZ)较厚,直接使用盐田日晒卤水,一般达2.0～2.0cm;运行周期短(约1个半月～2.月);升温幅度低(约3.～40℃);不需要复杂的辅助和维护设施。其成功运行为扎布耶盐湖锂资源的开发提供了热源。

关键词：扎布耶盐湖太阳池运行试验

A Novel Recommendation System With Collective Intelligence

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A Novel Recommendation System With Collective Intelligence

2.10 IEEE 2.d Symposium on Web Society

作者： ~(#1)Jia Zhou,~(#2.Tiejian Luo,~(*3.Haixiang Lin # School of Information Science and Engineering Graduate University of chinese academy of {2. beijing,china * Delft University of Technology,the Netherlands

＜正＞Academic resources on web include courses, educational videos,scientific literatures,experts,peers and all of the useful stuff for *** is crucial for researchers especially freshman to access and control the academic resources when they start and conduct a research *** paper proposes a recommendation system suggests high-quality materials to users according to their research *** system makes use of an ontology which is created by domain experts to define the categories of the entire research subjects. The materials of each category are recommended by domain experts and users which are called collective *** the recommended academic resources list（RARL） is updated adaptively with the operation of the *** on the results of user intention detection the system assign each user to the corresponding categories and the user gets his recommendation based on the content in *** the proposed system there are 15,000 education videos,12.GB related materials of 917 courses and 3.,000 web *** experimental tests show that the system performance is well:the performance is not getting worse when there are more web pages.

关键词： Academics Ontology Recommended Academic Resources List(RARL) Recommendation System Collective Intelligence Adaptive User Intention Detection

Allelopathic interference of the essential oil from wild oat (Avena fatua) and crabgrass (Digitaria sanguinalis) with wheat (Triticum aestivum)

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Allelopathic interference of the essential oil from wild oat...

中国第五届植物化感作用学术研讨会

作者： ZHOU Bin 1,WANG Peng 1,KONG Chui-Hua 2.1 Institute of Applied Ecology,chinese academy of {2.,Shenyang 110016,china 2.College of Resources and Environmental {2.,china Agricultural University,beijing 100193.china

Crop-weed allelopahtic interactions may be mediated by the volatile allelochemicals released from crop,weed or *** this study,we analyzed chemical constituents of the essential oils from two weeds wild oat (Avena fatua) and crabgrass (Digitaria sanguinalis) and to evaluate their effects on the growth and allelochemical production of wheat (Triticum aestivum).Subsequently,we identified 51 constituents in the essential oil of crabgrass and 2. chemical constituents in that of wild oat by means of GC and GC-MS combination *** crabgrass the main constituent was hexadecyl-oxirane ( 9.04 %).And Henicosane (15.05 %) was the lagest one presented in wild *** the oils affected the growth of wheat with different growth media including water,soil and *** highest shoot dried weight inhibition rate is 2..58 % with the the aqueous solution of volatile oil on filter,and in soil root dried weight inhibition rate arrives at 3..43.%.In particular,the biomass allocation of wheat was changed greatly in aqueous growth ***,the oil of crabgrass changed biomass allocation more significantly than wild oat ***,the essential oils of two weeds induced the production of allelochemical DIMBOA (2.4-Dihydroxy-7-methoxy-1,4Benzoxazin-3.one) in *** DIMBOA content in wheat changed with the induction *** increase was observed at early induction time then declined *** the increase of the induction time,the DIMBOA content in wheat *** the time of 2.h,DIMBOA content wheat exposed to the volatiles of crabgrass or wild oat arrived at the *** points of them were 0.47 mg/g and 0.48 mg/g *** to the plain air the highest DIMBOA content of wheat was 0.3. mg/*** results suggested that allelopathic interference of the essential oils from wild oat and crabgrass with wheat occurred not only on biomass allocation but also on allelochemical production.

关键词：

DEFORMATION OF THE EASTERN HIMALAYAN SYNTAXIS: EVIDENCES FROM STRUCTURES AND KINEMATICS OF ITS WESTERN BOUNDARY

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地学前缘 2000年第S1期7卷 18-20页

作者： Zhang J.,Zhong D.,Ding L.,Ji J.,He S(Institute of Geology and Geophysics, chinese academy of {2., {3. 10002.,china)

The western boundary of the Eastern Himalayan Syntaxis (EHS) is a deformation belt up to 3.km wide (Fig.1). Trending ca. N3.°E, it separates the Gangdise magmatic belt in the west from the gneiss of EHS in the east. Its rock association, mica\|schist, quartzite, marble, and amphibolite, can be traced to the south to Gangdise belt and they were probably metamorphosed from the sediments along Yarlung Zangbo. This belt consists of several intensive deformation zones, the largest one of which is along the belt’s western margin from Dongjug to Mainling and we called this ca. 10km wide shear zone as the Dongjug\|Mainling shear zone (DMSZ).DMSZ experienced earlier ductile shear and later ductile\|brittle normal faulting. The earlier deformation produced mylonitic rocks. Their foliation trends N3.°～40°E and dips northwest with the angle ranging from 55°to 80°, steepening northeastward. The penetrative kinematic lineation in the rocks has a varying attitude along the trend of DMSZ. It dips southwest with an angle of ca.3.° in the southwest near Mainling, whereas dips northeast in the northeast. Moreover, the northeast dipping lineation steepens northeastwards, e.g., its angle ranges form 3.° to 45° in the segment from Serkyim La to Dongjug but becomes 60～70° in the northeast most in another zone near Parlung. Kinematic indicators show that the motion of DMSZ had a left\|lateral slipping component, but the vertical motion components were different in the southwest from the northeast. From Serkyim La to the northeast, DMSZ had a kinematics of NW plate (Gangdise belt) thrusting over the SE plate (EHS) and its thrusting component increased toward northeast. However, the DMSZ has a vertical motion with the SE plate (EHS) as the uplifting plate.

关键词： Eastern Himalayan Syntaxis indentation uplift

A New Caffeate from the Aerial Parts of Anabasis aphylla

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chinese Herbal Medicines 2010年第4期2卷 248-249页

作者： LI Wei-lin1, 3. WANG Zhen-zhong2. WANG Hong-qing1, GONG Ting1, CHEN Ruo-yun1 1. Key Laboratory of Bioactive Substances and Resources Utilization of chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, chinese {2. of Medical sciences and Peking Union Medical College, beijing 100050, china 2. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 2.2.01, china 3. Department of Pharmacology, Pharmacy College of Xinxiang Medical College, Xinxiang 453.03. china Key Laboratory of Bioactive Substances and Resources Utilization of chinese Herbal Medicine Ministry of EducationInstitute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China Department of Pharmacology Pharmacy College of Xinxiang Medical College Xinxiang 453003 China Jiangsu Kanion Pharmaceutical Co. Ltd. Lianyungang 222001 China Key Laboratory of Bioactive Substances and Resources Utilization of chinese Herbal Medicine Ministry of EducationInstitute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

Objective To study the chemical constituents from the aerial parts of Anabasis aphylla. Methods The chemical constituents were isolated and purified by silica gel column and Sephadex LH-2. column chromatography. Spectroscopic methods such as MS and NMR spectra were used for the structural identification. Results A new caffeate ester, named eicosyl-(Z)-caffeate (1), along with fourteen known compounds was isolated from the EtOAc part. Conclusion Compound 1 is a new compound and compounds 2.13.are isolated from the plants of Anabasis L. for the first time.

关键词： Anabasis L. Anabasis aphylla L eicosyl-(Z)-caffeate

微卫星标记对黑龙江流域大麻哈鱼遗传多样性的研究

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水生生物学报 2004年第6期28卷 607-612页

作者：陈金平董崇智孙大江王喆张树义中国科学院动物研究所北京100080 中国水产科学研究院黑龙江水产研究所哈尔滨150080 中国水产科学研究院鲟鱼繁育技术工程中心北京100039

采用 12.微卫星标记 ,对中国 3.大麻哈鱼洄游群体 (乌苏里江、黑龙江和绥芬河 )的遗传多样性进行了检测。计算出各个种群的基因杂合度、遗传多样性和各个座位的多态信息含量。结果表明 ,3.大麻哈鱼洄游种群的平均基因杂合度分别为 :0... 详细信息

采用 12.微卫星标记 ,对中国 3.大麻哈鱼洄游群体 (乌苏里江、黑龙江和绥芬河 )的遗传多样性进行了检测。计算出各个种群的基因杂合度、遗传多样性和各个座位的多态信息含量。结果表明 ,3.大麻哈鱼洄游种群的平均基因杂合度分别为 :0 .6 73.0}0 5 995、0 .6 917,种群遗传多样性分别为 0 .70 82.0 .6 5 11、0 .76 16。这些结果表明大麻哈鱼遗传多样性还比较丰富 ,其资源的恢复具有良好的前景 ,说明当前中国大麻哈鱼资源数量下降并非由遗传因素引起 ,主要原因可能是由于过度捕捞和水域环境污染等人为因素造成。人工增殖放流为恢复中国大麻哈鱼资源起到了重要作用。

关键词：微卫星标记大麻哈鱼遗传多样性种群黑龙江流域

Characterization of the oligomerization of influenza A virus Ml protein

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Characterization of the oligomerization of influenza A virus...

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Ke Zhang~(1,4).Zhao Wang~2.Xiaoling Liu~1,Changcheng Yin~2.Bin Xia~3.Wenjun Liu~1 1.Center for Molecular Virology,CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,chinese {3. of {2.,beijing 100101,china 2.Department of Biophysics,Health Science Center,Peking University,beijing 100191,china 3.beijing Nuclear Magnetic Resonance Center,Peking University,beijing 100871,china 4.Graduate University of chinese {3. of {2.,beijing 100101,china

Influenza A virus matrix 1 protein(Ml) is a multifunctional protein and it plays an important role in virus life ***,structural and functional studies suggest that self-association of M1 is essential for assembly and ... 详细信息

关键词： Ml oligomerization