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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, chinese academy of sciences, Wuhan 4.0072. china 2. Graduate {2. of chinese academy of sciences, beijing 100039, china) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate school of chinese academy of sciences Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4.amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334.aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4.bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4.month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4.month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4.month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

An improved method for evaluating the seasonal variability of total suspended sediment flux field in the Yellow and East china Seas

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International Journal of Sediment Research 2011年第1期26卷 1-14页

作者： PANG Chongguang~1,YU Wei~2.YANG Yang~3,and HAN Danxiu~4.Institute of Oceanology,chinese academy of sciences,Qingdao 2.6071,china, 2.Institute of Oceanology,chinese academy of sciences,Qingdao 2.6071,china,Graduate school of chinese academy of sciences,beijing 100039,china 3 Institute of Oceanology,chinese academy of sciences,Qingdao 2.6071,china,Graduate school of chinese academy of sciences,beijing 100039,china 4.china University of Petroleum,beijing 102.4.,china Doctor Researcher Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of Chinese Academy of Sciences Beijing 100039 China china University of Petroleum Beijing 102249 China

The suspended sediment flux field in the Yellow and East china Seas(YECS) displays its seasonal variability.A new method is introduced in this paper to obtain the flux field via retrieval of ocean color remote sensing data,statistical analysis of historical suspended sediment concentration data,and numerical simulation of three-dimensional(3D) flow *** components of the sediment flux field include(i) surface suspended sediment concentration inverted from ocean color remote sensing data;(ii) vertical distribution of suspended sediment concentration obtained by statistical analysis of historical observation data;and(iii) 3D flow field modeled by a numerical *** the improved method,the 3D suspended sediment flux field in the YECS has been *** comparison with the suspended sediment flux field solely based on the numerical simulation of a suspended sediment transport model,the suspended sediment flux field obtained by the improved method is found to be more *** 3D suspended sediment flux field from ocean colour remote sensing and in situ observation are more closer to the ***,by quantitatively analyzing the newly obtained suspended sediment flux field,the quantity of sediment erosion and deposition within the different regions can be *** sediment exchange between the Yellow Sea and the East china Sea can be *** mechanism of suspended sediment transport in the YECS can be better *** particular,it is suggested that the long-term transport of suspended sediment is controlled mainly by the circulation pattern,especially the current in winter.

关键词： Suspended sediment flux field Seasonal variability Yellow and East china Seas Regression equation Numerical simulation

Structures and physical properties of rigid polyurethane foams with water as the sole blowing agent

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Science china Chemistry 2006年第4期49卷 363-370页

作者： LI Xiaobin1,2. CAO Hongbin1 & ZHANG Yi1 1. Institute of Process Engineering, chinese academy of sciences, beijing 100080, {2. 2. Graduate school of the chinese academy of sciences, beijing 100039, {2. Institute of Process Engineering Chinese Academy of Sciences Beijing China Graduate school of the chinese academy of sciences Beijing China

Rigid polyurethane foams blown by varying water level were prepared in this study. The structures and physical properties of rigid polyurethane foams blown by water were measured with FT-IR, universal testing machine (Instron3365), scanning electron microscope (SEM) and differential scanning calorimeter (DSC). The results show that polyurea and polybiuret were the typical charac- teristics, and the cream time and gel time were shorter for the fully water blown rigid foams than that for the fully cyclopentane blown foams. The density of foam samples decreased from 4..0 kg/m3 to 2..4.kg/m3 with the increase of water level from 3 pph to 7 pph. Compressive strength exhibited the similar behavior with density. The average cell size of foam samples ranged from 2.1 μm to 356 μm with the increase of water level from 3 pph to 7 pph, respectively. At the same time, poorer dimen- sional stability was encountered with the increase of average cell size due to fast diffusion rate of CO2.out of the foam. The results of thermal analysis show that the glass transition temperature (Tg) shifted to a higher temperature with the increase of isocyanate level when more water was used as chemical blowing agent.

关键词： polyether polyol, polyurethane rigid foam, compressive strength, dimensional stability, cell size, glass transition temperature.

Analysis and application of automatic deformation monitoring data for buildings and structures of mining area

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中国有色金属学会会刊：英文版 2011年第S3期21卷 516-522页

作者： XIAO Jie1, 2. 3, ZHANG Jin4.1. Institute of Geodesy and Geophysics, chinese academy of sciences, Wuhan 4.0077, china 2. Key Laboratory of Dynamic Geodesy, Institute of Geodesy and Geophysics, chinese academy of sciences, Wuhan 4.0077, china 3. Graduate school of chinese academy of sciences, beijing 10004., china 4. Department of Surveying and Mapping, Taiyuan University of Technology, Taiyuan 03002., china Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China Key Laboratory of Dynamic Geodesy Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China Graduate school of chinese academy of sciences Beijing 100049 China Department of Surveying and Mapping Taiyuan University of Technology Taiyuan 030024 China

The buildings and structures of mines were monitored automatically using modern surveying technology. Through the analysis of the monitoring data, the deformation characteristics were found out from three aspects containing points, lines and regions, which play an important role in understanding the stable state of buildings and structures. The stability and deformation of monitoring points were analysed, and time-series data of monitoring points were denoised with wavelet analysis and Kalman filtering, and exponent function and periodic function were used to get the ideal deformation trend model of monitoring points. Through calculating the monitoring data obtained, analyzing the deformation trend, and cognizing the deformation regularity, it can better service mine safety production and decision-making.

关键词： wavelet analysis Kalman filtering deformation monitoring data analysis mine

Wind vector retrieval using dual polarization imagery of ASAR

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Progress in Natural Science:Materials International 2006年第11期16卷 1183-1187页

作者： SONG Guiting~ 1,2., HOU Yijun~1** and QI Peng~1(1. Institute of Oceanology, chinese academy of sciences, Qingdao 2.6071, china 2. Graduate {2., chinese academy of sciences, beijing 100039, china) Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate school Chinese Academy of Sciences Beijing 100039 China

A new algorithm for retrieving wind vector using dual polarization imagery of advanced synthetic aperture radar (ASAR) is developed and tested. Based on the combination of co-polarization and cross-polarization, this new algorithm effectively eliminates the 180° ambiguity which occurs when using single imagery of the ASAR to retrieve wind vector. This algorithm also solves the problem that the retrieval results will break down on very small spatial scales. The results retrieved from dual polarization imagery of ASAR show that the wind speed and direction are in agreement with the data from Quikscat and buoy measurements. The root mean square errors of wind direction and speed between the retrieved results and data from Quikscat are 2.2.° and 0.53 m/s, respectively. Comparisons between the new method and the traditional method show that the new methods fit the buoy data and Quikscat data a little better than the traditional method in this image.

关键词： wind vector retrieval ASAR dual polarization.

A novel operation mode for spaceborne polarimetric SAR

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Science china(Information sciences) 2011年第4期54卷 884-897页

作者： QI WeiKong 1,2.& YU WeiDong 1 1 Institute of Electronics,chinese academy of sciences,beijing 100190,{2. 2.Graduate school of chinese academy of sciences,beijing 100039,{2. Institute of Electronics Chinese Academy of Sciences Beijing China Graduate school of chinese academy of sciences Beijing China

A novel operation mode for spaceborne polarimetric SAR,named polarization space-division adding polarization code-division (PSD-PCD) operation mode,is proposed in this paper through combining polarimetric SAR with elevation digital beam-forming (DBF) on *** a pulse repetition interval the up-chirp LFM sub-pulse is transmitted with horizontal polarization antenna,and with some delay the down-chip LFM subpulse is transmitted with vertical polarization antenna to realize code *** every sub-aperture receives returns from ground,SAR system uses range DBF to separate echoes relative to different sub-pulses in order to realize space *** system PRF is equal to that of single-polarization SAR,and elevation DBF help reduce range ambiguity ***,the main system parameters,including PRF,delay between two sub-pulses,radar equation and ambiguity are analyzed and compared with those of single-polarization *** results demonstrate the feasibility and validity of PSD-PCD operation mode.

关键词： spaceborne synthetic aperture radar (SAR) polarimetry digital beamforming (DBF) operation mode ambiguity ratio polarization space-division polarization code-division

Recent advances in the data analysis method of functional magnetic resonance imaging and its applications in neuroimaging

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Progress in Natural Science:Materials International 2006年第8期16卷 785-795页

作者： TIAN Jie, YANG Lei and HU Jin (Institute of Automation, chinese academy of sciences, beijing 100080, {2. Graduate school of chinese academy of sciences, beijing 100039, {2.) Institute of Automation Chinese Academy of Sciences Beijing 100080 China Graduate school of chinese academy of sciences Beijing 100039 China

Functional magnetic resonance imaging (fMRI) has opened a new area to explore the human brain. The fMRI can reveal the deep insights of spatial and temporal changes underlying a broad range of brain function, such as motor, vision, memory and emotion, all of which are helpful in the clinical investigation. In this paper, we introduce some recent-developed algorithms for fMRI signal detection such as model-driven method (general linear model, deconvolution model, non-linear model, etc. ) and data-driven method (principle component analysis, independent component analysis, self-organization mapping, clustered constrained non-negative matrix factorization, etc. ). We also propose several important applications of neuroimaging and point out their shortcomings and future perspectives.

关键词： fMR1 data analysis neuroimaging.

Preparation and characterization of a novel adsorbent for removing lipophilic organic from water

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Science china Chemistry 2005年第6期48卷 600-604页

作者： LIU Huijuan1, DAI Ruihua1, QU Jiuhui1 & RU Jia1,2.1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental sciences, chinese academy of sciences, {2. 100085, {4. 2. Graduate school of the chinese academy of sciences, beijing 100039, {4. State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China Graduate school of the chinese academy of sciences Beijing China

A novel composite adsorbent containing a kind of lipid-triolein is studied. The ad-sorbent is prepared by embedding triolein into cellulose acetate (CA) sphere. The preparation method, the physical-chemical properties of the adsorbent and the removal efficiency of two or-ganochlorinated pesticides are studied. The adsorbent is stable in water and no triolein leaks into water for 4.5 h soaking. The adsorbent has high adsorption capacity for organochlorinated pes-ticides such as dieldrin and aldrin. The results suggest that triolein-containing adsorbent could serve as a good adsorbent for lipophilic organic pollutants. The adsorption rate for lipophilic pol-lutants is very fast and has relation with the logKow of the compounds.

关键词： triolein, cellulose acetate, adsorbent, lipophilic organic, drinking water.

Floating Population Increase and Its Influence on the Urban Population Situation:A Case Study in beijing

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chinese Journal of Population,Resources and Environment 2005年第1期3卷 27-35页

作者： Wu Peilin 1,2.& Lu Qi 2.1. Shandong University of Technology, Zibo Shandong 2.504., china 2. Institute of Geographical sciences and Natural Resources Research, {2., beijing 100101, china Shandong University of Technology Zibo Shandong 255049 China Institute of Geographical sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China

Floating population is a special population group in china resulting from the implementation of household registration system. This paper uses a set of floating population survey data, population censuses data and statistical data to analyze the increase and influence of floating population on the urban population situations in beijing. It is found that beijing has experienced a rapid increase of floating population since the 1990s and that the increase of this group has become the key factor of the current population expansion in the city. Its distribution in the urban regions intensified and extended the suburbanization process of the capital. In addition, the population structures of sex, age, education and employment in beijing have changed to some extent due to the influx of floating population.

关键词： Floating population Household registration system (Hukou) Population expansion Suburbanization Population structure beijing