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Analysis of tandem repeats in the genome of chinese shrimp Fenneropenaeus chinensis

chinese Science Bulletin 2005年第14期50卷 1462-1469页

作者： KONG Jie1 & GAO Huan2.3.1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, chinese academy of Fishery {2., Qingdao 2.6071, china 2. Institute of Oceanology, chinese academy of {2., Qingdao 2.6071, china 3. The Graduate school of the chinese academy of {2.,{12., china Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheris Research Institute Chinese Academy of Fishery Sciences Qingdao China Institute of Oceanology Chinese Academy of Sciences Qingdao China The Graduate school of the chinese {2. of {3. Beijing China

Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2.59 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 2..6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2. of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42.. In decreasing order, the 2. most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (2.4), AAT (92., A (56), AAG (2.), ATC (2.), ATAG (2.), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them.

关键词：虾遗传特性遗传基因 DNA

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SHRIMP zircon U-Pb geochronology of early Mesozoic felsic igneous rocks from the southern Lancangjiang and its tectonic implications

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Science china Earth sciences 2006年第10期49卷 1032-1042页

作者： PENG Touping1,2. WANG Yuejun1, FAN Weiming1, LIU Dunyi3. SHI Yuruo3.& MIAO Laicheng4 1. Key Laboratory of Isotope Geochronology and Geochemistry, Guangzhou Institute of Geochemistry, chinese academy of {2., Guangzhou 510640, china 2. Graduate University of chinese academy of {2., beijing 10003., china 3. SHRIMP isotope Laboratory, chinese academy of Geological {2., beijing 10003., china 4. Institute of Geology and Geophysics, chinese academy of {2., beijing 10002., china Key Laboratory of Isotope Geochronology and Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China Graduate University of chinese academy of {2. Beijing China SHRIMP isotope Laboratory Chinese Academy of Geological Sciences Beijing China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China

The SHRIMP zircon U-Pb geochronology of three typical samples, including two monzo nitic granites from the Lincang batholith and a rhyolite from the Manghuai Formation are presented in the southern Lancangjiang, western Yunnan Province. The analyses of zircons for the biotite monzonitic granites from the northern (02.X-13.) and southern (2.JH-10) Lincang batholith show the single and tight clusters on the concordia, and yield the weighted mean 2.6Pb/2.8U ages of 2.9.4 ± 3.0 Ma and 2.0.4 ± 3.6 Ma, respectively, representing the crystallized ages of these granites. The zircons for the rhyolitic sample (02.X-95) from the Manghuai Formation give a weighted mean 2.6Pb/2.8U age of 2.1.0 ± 5.0 Ma. These data suggest that the igneous rocks from the Lincang granitic batholith and Manghuai Formation have a similar crystallized age. In combination with other data, it is inferred that both were generated at a narrow age span (~2.0 Ma) and were originated from the postcollisional tectonic regime. An early Proterozoic 2.6Pb/2.8U apparent age of 1977±44 Ma is additionally obtained from one zircon from the biotite monzonitic granite (southern Lincang batholith), indicative of devel- opment of the early Proterozoic Yangtze basement in the region. These precisely geochronological data provide important constraints on better understanding the Paleozoic tectonic evolution of the Tethys, western Yunnan Province.

关键词： SHRIMP zircon U-Pb dating, Middle Triassic, Lincang granitic batholith, Manghuai Formation rhyolite, southern Lancangjiang.

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Effect of rock mass structure and block size on the slope stability——Physical modeling and discrete element simulation

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Science china(Technological sciences) 2005年第Z1期48卷 1-17页

作者： LI Shihai1, LIAN Zhenzhong1,2.& J. G. Wang3.1. Institute of Mechanics, chinese academy of {2., beijing 100080, china 2. Graduate school, chinese academy of {2., beijing 100060, china 3. Tropical Marine Science Institute, National University of Singapore, Singapore 1192.0 Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China Graduate school Chinese Academy of Sciences Beijing 100060 China Tropical Marine Science Institute National University of Singapore Singapore 119260 Singapore

This paper studies the stability of jointed rock slopes by using our improved three-dimensional discrete element methods (DEM) and physical modeling. Results show that the DEM can simulate all failure modes of rock slopes with different joint configurations. The stress in each rock block is not homogeneous and blocks rotate in failure development. Failure modes depend on the configuration of joints. Toppling failure is observed for the slope with straight joints and sliding failure is observed for the slope with staged joints. The DEM results are also compared with those of limit equilibrium method (LEM). Without considering the joints in rock masses, the LEM predicts much higher factor of safety than physical modeling and DEM. The failure mode and factor of safety predicted by the DEM are in good agreement with laboratory tests for any jointed rock slope.

关键词： rock masses, slope stability, DEM simulation, block size effect, joints configuration effect, limit equilibrium method.

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Formal Verification under Unknown Constraints

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Wuhan University Journal of Natural sciences 2005年第1期10卷 43-46页

作者： LI Guang-hui 1,2.3., LI Xiao-wei 2.3.. school of Information Engineering, Zhejiang Forestry College, Hangzhou 3.13.0, Zhejiang,{3. 2. Institute of Computing Technology, {2. academy of sciences, beijing 100080,{3. 3. Graduate school of the {2. academy of sciences, beijing 10003.,{3. school of Information Engineering Zhejiang Forestry College Hangzhou Zhejiang China Institute of Computing Technology Chinese Academy of Sciences Beijing China Graduate school of the chinese {2. of {3. Beijing China

We present a formal method of verifying designs with unknown constraints (e.g., black boxes) using Boolean satisfiability (SAT). This method is based on a new encoding scheme of unknown constraints, and solves the corresponding conjunctive normal form (CNF) ***,this method can avoid the potential memory explosion, which the binary decision diagram (BDD) based techniques maybe suffer from, thus it has the capacity of verifying large designs. Experimental results demonstrate the efficiency and feasibility of the proposed method.

关键词： formal verification unknown constraints black box Boolean satisfiability Boolean comparison

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A tentative study for the prediction of the CME related geomagnetic storm intensity and its transit time

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Science china(Technological sciences) 2005年第6期48卷 648-668页

作者： ZHAO Xinhua1,2.& FENG Xueshang1,3.1. SIGMA Weather Group, Key Laboratory of Space Weather, Center for Space Science and Applied Re-search, chinese academy of {2., beijing 100080, china 2. Graduate school of the chinese academy of {2., beijing 10003., china 3. school of Geophysics and Geoinformation Systems, china University of Geo{2., beijing 100083. china SIGMA Weather Group Key Laboratory of Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China Graduate school of the chinese {2. of {3. Beijing China school of Geophysics and Geoinformation Systems China University of Geosciences Beijing China

Using 80 CME-ICME events during 1997.1―2.02.9, based on the eruptive source locations of CMEs and solar magnetic field observation at the photosphere, a current sheet magnetic coordinate (CMC) system is established in order to study the propagation of CME and its geoeffectiveness. In context of this coordinate system, the effect of the eruptive source location and the form of heliospheric current sheet (HCS) at the eruptive time of CME on the geomagnetic storm intensity caused by CME and the CME’s transit time at the Earth is investigated in detail. Our preliminary conclusions are: 1) The geomagnetic disturbances caused by CMEs tend to have the so-called “same side-opposite side effect”, i.e. CMEs erupt from the same side of the HCS as the earth would be more likely to arrive at the earth and the geomagnetic disturbances associated with them tend to be of larger magnitude, while CMEs erupting from the opposite side would arrive at the earth with less probability and the corresponding geomagnetic disturbance magnitudes would be relatively weaker. 2. The angular separation between the earth and the HCS affect the corresponding disturbance intensity. That is, when our earth is located near the HCS, adverse space weather events occur most probably. 3. The erupting location of the CME and its nearby form of HCS will also affect its arrival time at the earth. According to these conclusions, in this context of CMC coordinate we arrive at new prediction method for estimating the geomagnetic storm intensity (Dstmin) caused by CMEs and their transit times. The application of the empirical model for 80 CME-ICME events shows that the relative error of Dst is within 3.% for 59% events with Dstmin≤?50 nT, while the averaged absolute error of transit time is lower than 10 h for all events.

关键词： coronal mass ejections, current sheet magnetic coordinate system, geomagnetic storm intensity, transit time, prediction method.

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Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2.3. Lei Gang1,2. Wuriyanghan Hada1,2. Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, {3. academy of {2., beijing 100101, china 2. Graduate school of {3. academy of {2.,{13., china 3. Xinjiang academy of Agricultural and Reclamation Science, Shihezi 83.0}00, china) National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Acad. of Sci. Beijing 100101 China Graduate school Chinese Acad. of Sci. Beijing 100039 China Xinjiang academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2.and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2.and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

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A mixed method for measuring low-frequency acoustic properties of macromolecular materials

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 729-739页

作者： LIU Hongwei1,YAO Lei1,3.ZHAO Hong1,3.ZHANG Jichuan2.3.& XUE Zhaohong2.1. National Key Laboratory of Acoustics,Institute of Acoustics,chinese {3. of {2.,beijing 100080,china 2. Institute of Chemistry,chinese {3. of {2.,beijing 100080,china 3. Graduate University of chinese {3. of {2.,beijing 100049,china National Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences Beijing China Graduate University of chinese academy of {2. Beijing China Institute of Chemistry Chinese Academy of Sciences Beijing China

A mixed method for measuring low-frequency acoustic properties of macro-molecular materials is presented. The dynamic mechanical parameters of materials are first measured by using Dynamic Mechanical Thermal Apparatus(DMTA) at low frequen-cies,usually less than 100 Hz; then based on the Principles of Time-Temperature Super-position (TTS),these parameters are extended to the frequency range that acousticians are concerned about,usually from hundreds to thousands of hertz; finally the extended dynamic mechanical parameters are transformed into acoustic parameters with the help of acoustic measurement and inverse analysis. To test the feasibility and accuracy,we measure a kind of rubber sample in DMTA and acquire the basic acoustic parameters of the sample by using this method. While applying the basic parameters to calculating characteristics of the sample in acoustic pipe,a reasonable agreement of sound absorp-tion coefficients is obtained between the calculations and measurements in the acoustic pipe.

关键词： low-frequency acoustic properties,dynamic mechanical properties,Principles of Time-Temperature Superposition,acoustic pipe,sound absorption coefficient.

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Effective solutions to a global 3. visual system in networking environments

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Science china Earth sciences 2005年第11期48卷 2032-2039页

作者： ZHANG Liqiang1,2. ZHANG Yan3. YANG Chongjun2. LIU Suhong1, REN Yingchao2. RUI Xiaoping2.& LIU Donglin2.1. State Key Laboratory of Remote Sensing sciences, school of Geography and Remote Sensing, {2. Normal University, {2. 100875, china 2. State Key Laboratory of Remote Sensing sciences, Institute of Remote Sensing Applications, chinese {13. of sciences, {2. 101001, china 3. Institute of Geographical sciences and Natural Resources Research, chinese {13. of sciences, {2. 101001, china State Key Laboratory of Remote Sensing sciences School of Geography and Remote Sensing Beijing Normal University Beijing China chinese academy of {2. State Key Laboratory of Remote Sensing SciencesInstitute of Remote Sensing Applications China Institute of Geographical sciences and Natural Resources Research Chinese Academy of Sciences China

The development of Web-based global 3. visual system has made progresses. However, there exist few mature and effective ways for managing, transmitting and visualizing massive spatial data. Based on the related work, the paper illustrates the ellipsoidal quadtree technique for rapid access multi-scale and multiple level geographical data, integrates the streaming with level-of-detail rendering method for transmitting the data on the network, and im-plements large-scale terrain surface simplification using M-band wavelet transforms and mul-tiresolution triangulations. We fulfil a web-based global terrain visual system using COM com-ponents on the basis of the above techniques. The system has a good prospect in the military simulation and city plans.

关键词： massive spatial data, streaming transmission, system architecture, M-band wavelet

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Interannual variability of Antarctic Oscillation and its influence on East Asian climate during boreal winter and spring

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Science china Earth sciences 2006年第5期49卷 554-560页

作者： FAN Ke1,2.3.& WANG Huijun2.1. Nansen-Zhu International Research Center, Institute of Atmospheric Physics, chinese {3. of {2., beijing 10002., china 2. Department of Atmospheric Science, Yunnan University, Kunming 650091, china 3. Graduate University of the chinese {3. of {2., beijing 100049, china Nansen-Zhu International Research Center Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China Department of Atmospheric Science Yunnan University Kunming China Graduate University of the chinese academy of {2. Beijing China

The interannual variability of Antarctic Oscillation (AAO) and its influence on East Asian climate during both boreal winter and spring are addressed. The results show that the positive AAO anomaly decreases the cold activity over East Asia during both boreal winter and spring. AAO-related barotropic meridional teleconnection from Antarctic to Arctic is found through analysis of mean me- ridional circulations. This meridional teleconnection is remarkable over Eurasia during boreal winter and over the Pacific Ocean during boreal spring. The results also show that zonal mean zonal wind at high latitudes in Southern Hemisphere has well positive correlation with that of Eurasia during boreal winter and has negative correlation with Pacific North American teleconnection (PNA) during boreal spring, which again display the meridional teleconnection. Thus, local meridional teleconnection is a possible linkage for interaction of circulations at mid-high latitudes between both hemispheres.

关键词： Antarctic Oscillation, Eurasian west wind, meridional teleconnection, mean meridional circulation.

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Modeling and verifying Web services driven by requirements:An ontology-based approach

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Science in china(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3.JIN ZHi1,2.& WU Budan1,4 1. academy of Mathematics and Systems Science, chinese academy of sciences, beijing 100080, china 2. Institute of Computing Technology, chinese academy of sciences, beijing 100080, china 3. Institute of Software, chinese academy of sciences, beijing 100080, china 4. Graduate University of chinese academy of sciences, beijing 100049, china academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing China Institute of Software Chinese Academy of Sciences Beijing China Institute of Computing Technology Chinese Academy of Sciences Bejing China Graduate University of chinese academy of {2. Beijing China

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

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