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GM-CSF-induced Ca_(2.) -activated non-voltage-dependent inwardly rectifying K_+ channel in murine peritoneal exudate macrophages

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Chinese Science Bulletin 1997年第11期42卷 947-952页

作者： ZHANG Xiaofeng, FENG Meifu, WU Caihong and ZHOU Pei’aiState Key Laboratory of Biomembrane and Membrane Biotechnology, Chinese Academy of sciences, beijing 100080, {2. 1 . In-stitute of Zoology, Chinese Academy of sciences, beijing 100080, {2. 2.College of Life sciences, peking university, beijing 100871, {2. State Key Laboratory of Biomembrane and Membrane Biotechnology Chinese Academy of Sciences Beijing 100080 China

GRANULOCYTE-MACROPHAGE colony-stimulating factor(GM-CSF),a member in the family ofglycoprotein cytokines,can not only act to stimulate proliferation of immature progenitors,butalso enhance differentiated functions of mature effector *** has two kinds of biologicaleffects on mature effector cells,*** effect and indirect *** latter is also

关键词： K+ channel patch clamp GM-CSF macrophage.

Inhibitory effect of picroside Ⅱ on hepatocyte apoptosis

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Acta Pharmacologica Sinica 2005年第6期26卷 729-736页

作者： Hua GAO, Ya-wei ZHOU~2.College of Chemistry and Molecular Engineering, peking university, beijing 100871, {2. College of Chemistry and Molecular Engineering Peking University Beijing China

Aim: To investigate the influence of picroside on hepatocyte apoptosis and its mechanism. Methods: Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-quantitative reverse transcription-PCR (RT-PCR) was used to analyze the expression of bcl-2.and bax genes. The content of manganese-superoxide dismutase (SOD) in liver mitochondria was detected by the Marland method. The content of malonic aldehyde (MDA) and the protein level in liver tissue were determined by thiobarbituric acid colorimetry and Lowry method. Results: Picroside decreased the levels of alanine aminotransferase and aspartate aminotransferase in the serum resulting from acute-liver injured mice induced with D-GalN and LPS; it also reduced the content of MDA, and thus, enhanced the activity of SOD. Picroside 0 mg/kg was found to protect hepatocytes against apoptosis in a dose-dependent manner; it up-regulated the expression of bcl-2.genes, thus increased the bcl-2.bax ratio. Conclusion: Picroside 鈪an protect hepatocytes against injury and prevent hepatocytes from apoptosis. It might by upregulating the bcl-2.gene expression and antioxidation.

关键词： picroside Ⅱ hepatocytes apoptosis oxidative stress bcl-2.genes bax genes

Numerical simulation of Meiyu front and the diagnosis of moist vorticity vector

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Progress in Natural Science:Materials International 2007年第11期17卷 1341-1347页

作者： Ping Fan1,2.and Luo Zhexian2.1. Institute of Atmospheric Physics, Chinese Academy of sciences, beijing 10002., {2. 2. Nanjing university of Information sciences & Technology, Nanjing 2.0044, {2.) Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Nanjing university of Information sciences & Technology Nanjing 210044 China

Moist vorticity vector is introduced to study the development and evolution of mesoscale convective vortex (MCV) in the Meiyu front precipitation with the Advanced Regional Predication System (ARPS). In this study, the heavy rainfall is simulated to investigate the genesis, development and dissipation of middle scale convective vortex, which occurred from 0000 UTC 3 July to 12.0 UTC 5 July over the Jianghuai River valley. Moist vorticity vector (MVV) has zonal, radial and vertical components in its 3D spatial distribution. The simulation shows that the vertical component of moist vorticity vector well demonstrates the spatial distribution characteristics of middle scale convective vortex, especially in the process of Meiyu front precipitation. Diagnosis upon zonal, radial averaged and mass-integrated quantities of MVV shows that its vertical component and the surface precipitating ratio are in phase with a correlation coefficient of 0.68, indicating that the vertical component of MVV is closely associated with the Meiyu front precipitation. In addition, the tendency of the vertical component of MVV is mainly determined by the interaction between the vorticity and the zonal and radial gradient of condensational or depositional heating.

关键词： moist vorticity vector mesoscale convective vortex Meiyu front precipitation

A novel endorectal RF coil for high resolution MRI and spectroscopy of the prostate

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Progress in Natural Science:Materials International 2005年第5期15卷 472-476页

作者： ZHANG Hongjie 1,2. SONG Xiaoyu1, WANG Xiaoying3, XU Pingfang1 and BAO Shanglian1*(1. beijing Key Laboratory of Medical Physics & Engineering, peking university, {2., china 2. Navy General Hospital, beijing 100037, china 3. peking university First Hospital, beijing 100034, china) beijing Key Laboratory of Medical Physics & Engineering Peking University Beijing 100871 China Navy General Hospital Beijing 100037 China peking university First Hospital Beijing 100034 China

The diagnosis of prostate diseases by magnetic resonance imaging (MRI) in vivo is sometimes difficult for the lower signal to noise ratio (SNR). To increase the SNR of the prostate image, we designed a RF coil that can be inserted into rectum and was named endorectal coil. The properties of RF coil were evaluated using a network analyzer. Moreover the images and spectroscopy of a special phantom were acquired and the results were compared to those of the commercial TORSO coil (G.E. Medical Systems, USA). Our coil gave a significantly higher SNR at the region of interest (ROI). The achieved high local SNR and resulting high spatial resolution would add more anatomic and biochemical information to the diagnosis of prostate diseases.

关键词： MRI RF coil endorectal coil.

Kinetic characteristics of continuous flow polymerase chain reaction chip: A numerical investigation

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Science china(Technological sciences) 2010年第7期53卷 1967-1972页

作者： WANG ShiYing1 & WANG Wei2.3 1 School of Physics, peking university, beijing 100871, {2. 2.Institute of Microelectronics, peking university, beijing 100871, {2. 3 National Key Laboratory of Science and Technology on Micro/Nano Fabrication, beijing 100871, {2. 1. School of Physics Peking University Beijing 100871 China2. Institute of Microelectronics Peking University Beijing 100871 China3. National Key Laboratory of Science and Technology on Micro/Nano Fabrication Beijing 100871 China

Continuous flow PCR (polymerase chain reaction) chip holds impressive advantages compared to micro chamber PCR chip. In order to have better understanding of kinetic characteristics of continuous flow PCR chip, a comprehensive mathematical model is presented in this paper, including melting, annealing and extension phases of a typical PCR process which has the essence of a convection-diffusion-reaction system. Using this model, we can simulate the PCR process in series of reaction cycles. Numerical results show that the average sample velocity plays a significant role in affecting the amplification efficiency. Also, appropriate combination of the PCR mixture is important for high-quality DNA amplification. Giving a large initial DNA concentration range, the continuous flow PCR scheme holds excellent real-time detection ability theoretically. The present numerical model bridges the temperature distribution to the real DNA amplification, and thereby is able to successfully predict continuous flow PCR properties which are important for the chip design.

关键词： continuous flow PCR chip numerical simulation convection-diffusion-reaction system

Size-dependent hydroxyl radicals generation induced by SiO_2.ultra-fine particles: The role of surface iron

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Science china Chemistry 2009年第7期52卷 1033-1041页

作者： SHANG Yu1, ZHU Tong1, LI Yi1,2.& ZHAO JinCai3 1 College of Environmental sciences and Engineering, peking university, {2., china 2.Chinese Academy of Meteorological sciences, {2., china 3 Institute of Chemistry, Chinese Academy of sciences, {2., china 1. College of Environmental sciences and Engineering Peking University Beijing 100871 China 2. Chinese Academy of Meteorological sciences Beijing 100871 China 3. Institute of Chemistry Chinese Academy of Sciences Beijing 100871 China

Oxidative stress and hydroxyl radicals (·OH) play important roles in adverse health effects caused by inhalable ambient air particles (PM10). The ultra-fine fraction of PM10 has been hypothesized as one of the critical contributors to ·OH generation. Both in vivo and in vitro studies have shown that ultra-fine particles (UFPs) or nano-particles generate more ·OH than larger particles with identical mass and composition. Both the surface area and surface-adsorbed redox-active metals have been suggested as factors to determine the oxidative capacity of UFPs. In this study, the ·OH-generating capability of dif-ferent sizes of SiO2.particles was investigated in order to determine which factor influences particle-induced ·OH generation. The amount of ·OH generated in both acellular and cellular systems was quantified using a capillary electrophoresis method following exposure to SiO2.particles with diameters of 14, 100, and 500 nm. The amount of ·OH was strongly dependent on particle size, and a significant enhancement was observed only with 14 nm particles. Further studies indicated a close association between ·OH and iron ion concentration (R2.= 0.812. p2.particles is presented as a possible mechanism of UPFs-induced ·OH generation. The SiO2.acted as an inert substrate, and the surface of ultra-fine SiO2.particles acted as a carrier for iron.

关键词： ultra-fine particles nano-particles ·OH oxidative stress size-effect

Frontal genesis of moisture atmosphere during the early 2.08 persistent freezing-rain event in southern china

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Science china Earth sciences 2010年第3期53卷 454-464页

作者： QIAN WeiHong1 & FU JiaoLan1,2.1 Department of Atmospheric sciences, School of Physics, peking university, {2., china 2.National Meteorological Center of CMA, beijing, 100081, china 1. Department of Atmospheric sciences School of Physics Peking University Beijing 100871 China2. National Meteorological Center of CMA Beijing 100081 China

From January 10 to February 2. 2.08, a severe and persistent freezing-rain event occurred in southern and southwestern china. Here we use an observational analysis to compare the persistent freezing-rain event in the early 2.08 with the winter precipita- tion in the late 2.07 over south of the Yangtze River (Jiangnan). The persistent freezing-rain event was directly linked to the activity of quasi-stationary front. The gradient of equivalent temperature (ET) can well indicate the frontal genesis of moist atmosphere (moisture front) and its activity as well as its relationship with precipitation belt. The precipitation types (snow and freezing rains) are related to the vertical structure of moisture front. The inversion profile of ET vertical distribution is a typical synoptic condition that caused the freezing-rain event. The horizontal gradient of ET with a criterion of 10℃ / 100 km, which reflects the accumulation and release of atmospheric energy, can be applied to predict the precipitation 5-10 days in advance.

关键词： freezing-rain event frontal genesis extended-range weather forecast winter Jiangnan

Strengthening of the boreal winter Hadley circulation and its connection with ENSO

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Progress in Natural Science:Materials International 2007年第11期17卷 1327-1333页

作者： Ma Jie1,2.and Li Jianping2.1. College of Atmospheric sciences, Lanzhou university, Lanzhou 730000, {2. 2. LASG, Institute of Atmospheric Physics, Chinese Academy of sciences, beijing 10002., {2.) College of Atmospheric sciences Lanzhou University Lanzhou 730000 China LASG Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China

Empirical orthogonal function (EOF) analysis is carried out for the year-to-year variability of the boreal winter (DJF) mass stream function of the mean meridional circulation (MMC) during the period 1948—2.05. The results demonstrate that it is dominated by the equatorially asymmetric and symmetric modes. Further analysis shows that the former mode is linked with the boreal winter Hadley cell mainly on the decadal time-scale, and the latter on the interannual time-scale. The asymmetric mode index (AMI) with a clear upward trend contributes to the decadal strengthening of the boreal Hadley circulation, and is closely correlated with the tropical SST warming, especially in the region of Indo-west Pacific warm pool (INWP). Furthermore, the AMI also contributes to the abrupt change of the correlation coefficient between the boreal Hadley circulation and ENSO after 1976. The symmetric mode index (SMI) with robust and stable linkage with ENSO shows a significant interannual variability, suggesting that the variability of the Hadley circulation is mainly associated with ENSO on the interannual time-scale.

关键词： the Hadley circulation interannual time-scale decadal time-scale

Dynamic mechanisms of the post-seismic deformation following large events:Case study of the 1999 Chi-Chi earthquake in Taiwan of china

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Science china Earth sciences 2009年第11期52卷 1813-1824页

作者： ZHU ShouBiao1,2.& CAI YongEn2.1 Institute of Crustal Dynamics, china Earthquake Administration, beijing 100085, china 2.Department of Geophysics, peking university, beijing 100871, china 1. Institute of Crustal Dynamics China Earthquake Administration Beijing 100085 China 2. Department of Geophysics Peking University Beijing 100871 China

The mechanism of postseismic deformation related to strong earthquakes is important in geodynamics, and presumably afterslip or viscoelastic relaxation is responsible for the postsesimic deformation. The 1999 Chi-Chi, Taiwan of china, earthquake occurred in the region where GPS observation station is most densely deployed in the world. The unprecedented GPS data provides a unique opportunity to study the physical processes of postseismic deformation. Here we assume that the interactions of viscoelastic relaxation, afterslip, fault zone collapse, poroelastic rebound, flow of underground fluids, and all these combined contribute to the surface displacements following the main shock. In order to know the essence of the postseismic deformation after the strong event, fault zone collapse, poroelastic rebound, flow of underground fluids, and so on, are represented equivalently by the variations of the focal medium properties. Therefore, the viscoelastic relaxation, afterslip, and the variations of the equivalent focal medium properties are inverted by applying the GPS temporal series measurement data with viscoelastic finite element method. Both the afterslip rate distribution along the fault and the afterslip evolution with time are obtained by means of inversion. Also, the preliminary result suggests that viscosities of the lower crust and the upper mantle in Taiwan region is 2.7×1018 and 4.2.102. Pa·s, respectively. Moreover, the inversion results indicate that the afterslip contributing to postseismic deformation of 44.6% in 450 days after the Chi-Chi earthquake, with 34.7% caused by the viscous relaxation and 2..7% by other factors such as fault zone collapse, poroelastic rebound, and the flow of liquids.

关键词： mechanism of postseismic deformation viscoelastic finite element method GPS temporal series data Chi-Chi earthquake